Development of a Diagnostic Method for Distant Micrometastasis in Locally Advanced Colorectal Cancer Using ctDNA/RNA in Blood and Urinary Extracellular Vesicles

• Project/Area Number: 24K11877
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55020:Digestive surgery-related
• Research Institution: Nippon Medical School
• Principal Investigator: 上原 圭 日本医科大学, 医学部, 講師 (50467320)
• Co-Investigator(Kenkyū-buntansha): 山田 岳史 日本医科大学, 医学部, 准教授 (50307948)
• Project Period (FY): 2024-04-01 – 2027-03-31
• Project Status: Granted (Fiscal Year 2024)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2026: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2025: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2024: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 血中細胞外小胞 / 局所高度進行直腸癌 / 局所再発直腸癌 / ctDNA

Outline of Research at the Start

局所高度進行・局所再発直腸癌の血中細胞外小胞(extracellular vesicle: EV)に含まれるctDNAとlong non-cording RNA (lncRNA)を用いて遠隔微小転移を診断する方法を開発することを目的とする。数100-1000 nmの大きさを持つLarge extracellular vesicle (L-EV)を用いることで、検査の高感度化が期待できる。