| Project/Area Number |
24K17780
|
|---|
| Research Category |
Grant-in-Aid for Early-Career Scientists
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Basic Section 37010:Bio-related chemistry
|
|---|
| Research Institution | Kanazawa University |
|---|
Principal Investigator |
VU QUANG・CONG 金沢大学, ナノ生命科学研究所, 特任助教 (50961629)
|
|---|
| Project Period (FY) |
2024-04-01 – 2026-03-31
|
| Project Status |
Granted (Fiscal Year 2024)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2025: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2024: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
|
|---|
| Keywords | FLIM-GTP / GTP / bioimaging |
|---|
| Outline of Research at the Start |
Quantification of cellular GTP as an important metabolite in cellular metabolism will provide us the information to comprehend the energy status of cells. Here, we aim to develop a single fluorescent protein (FP)-based fluorescence lifetime biosensor for quantitative imaging of intracellular GTP level with fluorescence lifetime imaging microscopy (FLIM), denoted as FLIM-GTP. Using FLIM-GTP, we aim to quantify the GTP level in different cell types between normal and cancer cells, and to investigate the regulation function of GTP and heat production in brown adipocytes.
|
