| Project/Area Number |
24K18359
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 48010:Anatomy-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Li Xue (馬場雪) 国立研究開発法人理化学研究所, 生命機能科学研究センター, 研究員 (50936507)
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| Project Period (FY) |
2024-04-01 – 2026-03-31
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| Project Status |
Granted (Fiscal Year 2024)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2025: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2024: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | isotropic microscopy / single-protein-molecule / super resolution / imaging |
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| Outline of Research at the Start |
Recently, various super resolution techniques have been developed. Different from others, without expensive upgrade of microscopies and compromise on imaging speeds, expansion microscopy (ExM) can realize super resolution through physically expansion of samples that are embedded in a swellable hydrogel. This project aims to use ExM and further develop isotropic 10x ExM protocol that ensures preservation of structural features while improving the resolution for ten times. Furthermore, by combining with STED, this project plans to reach a resolution below 10 nm in 3D, both in cells and tissues.
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