DEL-1 Promotes Biogenesis of Mineralizing Extracellular Vesicles by Mediating Intracellular Calcium Signaling

• Project/Area Number: 24K19876
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Niigata University
• Principal Investigator: Rosenkranz Andrea 新潟大学, 医歯学系, 助教 (20985858)
• Project Period (FY): 2024-04-01 – 2026-03-31
• Project Status: Granted (Fiscal Year 2024)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2025: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2024: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: Calcium signaling / Extracellular vesicles / DEL-1

Outline of Research at the Start

Few studies have investigated how DEL-1 promotes bone growth and inhibits pro-resorptive activities. Established molecular biology and cell imaging, techniques will be used to show 1) how DEL-1 modulates intracellular Ca2+ signaling, 2) determine if DEL-1 induces EV formation by modulating intracellular Ca2+ signaling in osteoclasts and osteoblasts, and 3) if/how specific EV subpopulations from DEL-1 stimulated cells to promote calcification and bone growth.