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クライオ電子顕微鏡による生体分子モーターの立体構造と機能の解明

Research Project

Project/Area Number 25000013
Research Category

Grant-in-Aid for Specially Promoted Research

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionOsaka University

Principal Investigator

難波 啓一  大阪大学, 生命機能研究科, 特任教授 (30346142)

Co-Investigator(Kenkyū-buntansha) 川岸 郁朗  法政大学, 生命科学部, 教授 (80234037)
Project Period (FY) 2013 – 2018
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥575,510,000 (Direct Cost: ¥442,700,000、Indirect Cost: ¥132,810,000)
Fiscal Year 2017: ¥80,600,000 (Direct Cost: ¥62,000,000、Indirect Cost: ¥18,600,000)
Fiscal Year 2016: ¥82,680,000 (Direct Cost: ¥63,600,000、Indirect Cost: ¥19,080,000)
Fiscal Year 2015: ¥103,350,000 (Direct Cost: ¥79,500,000、Indirect Cost: ¥23,850,000)
Fiscal Year 2014: ¥119,080,000 (Direct Cost: ¥91,600,000、Indirect Cost: ¥27,480,000)
Fiscal Year 2013: ¥189,800,000 (Direct Cost: ¥146,000,000、Indirect Cost: ¥43,800,000)
Keywordsナノマシン / べん毛モーター / クライオ電子顕微鏡 / 単粒子像解析 / Cリング構造 / 回転スイッチ機構 / MSリング構造 / 輸送ゲート構造 / 分子モーター / 立体構造 / 光学顕微ナノ計測 / ブラウン・ラチェット / 生物物理 / ナノバイオ
Outline of Annual Research Achievements

べん毛モーターの回転機構の解明をめざして進めてきたべん毛基部体のクライオ電子顕微鏡による構造解析では、昨年から一層の進捗があった。信号タンパク質CheY-Pの非存在下では反時計回りにのみ回転する野生型モーターと、時計回りにのみ回転する変異型モーターの、2つの構造モデルを構築して精密化し、回転スイッチに関わる構造変化を明らかにしたとともに、モーター回転子のコア部分で膜貫通型タンパク質FliF複合体のMSリング、その中心部に結合する3種類の膜タンパク質FliP, FliQ, FliRからなる3型べん毛タンパク質輸送ゲートのらせん状複合体、そしてさらにその上に5種類のタンパク質FliE, FlgB, FlgC, FlgF, FlgGが順番にらせん状に重合してドライブシャフトとして働くロッドの構造についても、この巨大な複合体の全体構造を5Å分解能で解析し、各構成タンパク質の結晶構造やクライオ電顕による高分解能MSリング構造を組合わせて、分子モデルを構築することに成功した。両成果ともに投稿論文作成中である。
心筋のトロポミオシン・トロポニン・アクチン繊維からなる細いフィラメントのクライオ電顕による構造解析では、トロポミオシンの全長の分子モデルを初めて構築してトロポミオシン間のジャンクション構造を明らかにするとともに、トロポニンを構成するの3種類のサブユニットがアクチン繊維の極めて広い領域にわたって結合する様子を解明した。また、Caイオン存在下と非存在下における2状態の構造を明らかにし、筋収縮の制御のしくみを支える構造変化を明らかにした。
当該研究課題と並行して2010年から日本電子(株)と共同開発し、2016年夏にプロトタイプが完成した高度に自動化した新型クライオ電子顕微鏡CRYO ARM 200(加速電圧200kV)では、2017年夏に3日間の自動撮影で収集した約2500枚のβガラクトシダーゼの電顕像(35万粒子像)から2.4Å分解能を達成したが、その商用製品1号機として2018年6月に理研SPring-8センターに導入設置されたCRYO ARM 300(300kV)ではアポフェリチン球殻状構造のクライオ電顕像を撮影し、1日で自動撮影したわずか840枚の電顕像(12万粒子像)から、世界最高となる1.53Å分解能を達成した。この商用機はプロトタイプにはない冷陰極電界放射型電子銃(Cold FEG)が装備されているため電子線の干渉性がより高く、しかも300kVという高い加速電圧も寄与して電子光学系の解像度は1.0Å分解能に近いことがわかっている。今回得たアポフェリチンの3次元像マップでは、トリプトファン側鎖の五員環に孔が空き、メチオニン側鎖の炭素原子と硫黄原子のサイズに明確な違いが見えるなど、その解像度は原子分解能に近いもので、生体分子水溶液試料の構造解析における原子分解能の達成にむけて、Cold FEGを装備したクライオ電子顕微鏡の極めて大きなポテンシャルを示した。

Assessment Rating
Verification Result (Rating)

A

Assessment Rating
Result (Rating)

A: Progress in the research is steadily towards the initial goal. Expected research results are expected.

Report

(12 results)
  • 2019 Research Progress Assessment (Verification Result) ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report   Abstract(Research Progress Assessment) ( PDF )   Research Progress Assessment (Result) ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report   Research Progress Assessment (Comments on the Site Visits) ( PDF )
  • 2013 Abstract ( PDF )   Comments on the Screening Results ( PDF )   Annual Research Report
  • Research Products

    (427 results)

All 2019 2018 2017 2016 2015 2014 2013 Other

All Int'l Joint Research (24 results) Journal Article (66 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 66 results,  Open Access: 39 results,  Acknowledgement Compliant: 17 results) Presentation (309 results) (of which Int'l Joint Research: 30 results,  Invited: 79 results) Book (18 results) Remarks (7 results) Funded Workshop (3 results)

  • [Int'l Joint Research] オックスフォード大学/ニューカッスル大学/分子生物学研究所(イギリス)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ユタ大学(アメリカ合衆国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] フランス国立科学研究センター(フランス)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] メキシコ国立自治大学(メキシコ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ニューサウスウェールズ大学(オーストラリア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research]

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] オックスフォード大学/ブリストル大学/分子生物学研究所(英国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] ユタ大学(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] フランス国立科学研究センター(フランス)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] メキシコ国立自治大学(メキシコ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] ニューサウスウェールズ大学(オーストラリア)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research]

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] オックスフォード大学/ブリストル大学/分子生物学研究所(英国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] ユタ大学(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] フランス国立科学研究センター(フランス)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] メキシコ国立自治大学(メキシコ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] 欧州バイオインフォマティクス研究所オーストラリア支部(オーストラリア)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research]

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] オックスフォード大学/ブリストル大学/分子生物学研究所(英国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] ユタ大学(米国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] フランス国立科学研究センター(フランス)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] メキシコ国立自治大学(メキシコ)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] ビクター・テャン心臓病研究所(オーストラリア)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research]

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Novel Insights into Conformational Rearrangements of the Bacterial Flagellar Switch Complex2019

    • Author(s)
      Sakai Tomofumi、Miyata Tomoko、Terahara Naoya、Mori Koichiro、Inoue Yumi、Morimoto Yusuke V.、Kato Takayuki、Namba Keiichi、Minamino Tohru
    • Journal Title

      mBio

      Volume: 10 Issue: 2 Pages: 1-14

    • DOI

      10.1128/mbio.00079-19

    • NAID

      120006822482

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural insights into the substrate specificity switching mechanism of the type Ⅲ protein export apparatus.2019

    • Author(s)
      Inoue, Y., Ogawa, Y., Kinoshita, M., Shimada, M., Kodera, N., Ando, T., Namba, K, Kitao, A., Imada, K. & Minamino, T.
    • Journal Title

      Structure

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Insight into structural remodeling of the FlhA ring responsible for bacterial flagellar type III protein export2018

    • Author(s)
      N. Terahara, Y. Inoue, N. Kodera, Y. V. Morimoto, T. Uchihashi, K. Imada, T. Ando, K. Namba, and T. Minamino
    • Journal Title

      Sci. Adv.

      Volume: 4 Issue: 4

    • DOI

      10.1126/sciadv.aao7054

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] In Vitro Reconstitution of Functional Type III Protein Export and Insights into Flagellar Assembly2018

    • Author(s)
      Hiroyuki Terashima, Akihiro Kawamoto, Chinatsu Tatsumi, Keiichi Namba, Tohru Minamino, Katsumi Imada
    • Journal Title

      mBio

      Volume: 9(3) Issue: 3

    • DOI

      10.1128/mbio.00988-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Autonomous control mechanism of stator assembly in the bacterial flagellar motor in response to changes in the environment2018

    • Author(s)
      Minamino Tohru、Terahara Naoya、Kojima Seiji、Namba Keiichi
    • Journal Title

      Molecular Microbiology

      Volume: 109 Issue: 6 Pages: 723-734

    • DOI

      10.1111/mmi.14092

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A triangular loop of domain D1 of FlgE is essential for hook assembly but not for the mechanical function.2018

    • Author(s)
      Sakai, T., Inoue, Y., Terahara, N., Namba, K. & Minamino, T.
    • Journal Title

      BBRC

      Volume: 495 Issue: 2 Pages: 1789-1794

    • DOI

      10.1016/j.bbrc.2017.12.037

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Insight into adaptive remodeling of the rotor ring complex of the bacterial flagellar motor.2018

    • Author(s)
      Kinoshita, M., Furukawa, Y., Uchiyama, S., Imada, K., Namba, K. & Minamino, T.
    • Journal Title

      BBRC

      Volume: 496 Issue: 1 Pages: 12-17

    • DOI

      10.1016/j.bbrc.2017.12.118

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel insights into the mechanism of well-ordered assembly of bacterial flagellar proteins in Salmonella2018

    • Author(s)
      Inoue Yumi、Morimoto Yusuke V.、Namba Keiichi、Minamino Tohru
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 1787-1787

    • DOI

      10.1038/s41598-018-20209-3

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evidence for the hook supercoiling mechanism of the bacterial flagellum2018

    • Author(s)
      Takashi Fujii,Hideyuki Matsunami, Yumi Inoue and Keiichi Namba
    • Journal Title

      Biophysics and Physicobiology

      Volume: 15 Issue: 0 Pages: 28-32

    • DOI

      10.2142/biophysico.15.0_28

    • NAID

      130006337975

    • ISSN
      2189-4779
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tunnel Formation Inferred from the I-Form Structures of the Proton-Driven Protein Secretion Motor SecDF2017

    • Author(s)
      Arata Furukawa, Kunihito Yoshikaie, Takaharu Mori, Hiroyuki Mori, Yusuke V. Morimoto, Yasunori Sugano, Shigehiro Iwaki, Tohru Minamino, Yuji Sugita, Yoshiki Tanaka, Tomoya Tsukazaki
    • Journal Title

      Cell Reports

      Volume: 19 Issue: 5 Pages: 1-7

    • DOI

      10.1016/j.celrep.2017.04.030

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The role of intrinsically disordered C-terminal region of Flik in substrate specificity switching of the bacterial flagellar type III export apparatus.2017

    • Author(s)
      Kinoshita, M., Aizawa, S., Inoue, Y., Namba, K. & Minamino, T.
    • Journal Title

      Mol. Microbiol.

      Volume: 105 Issue: 4 Pages: 572-588

    • DOI

      10.1111/mmi.13718

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assembly and stoichiometry of the core structure of the bacterial flagellar type III export gate complex2017

    • Author(s)
      Fukumura Takuma、Makino Fumiaki、Dietsche Tobias、Kinoshita Miki、Kato Takayuki、Wagner Samuel、Namba Keiichi、Imada Katsumi、Minamino Tohru
    • Journal Title

      PLOS Biology

      Volume: 15 Issue: 8 Pages: e2002281-e2002281

    • DOI

      10.1371/journal.pbio.2002281

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Implications of coordinated cell-body rotations for Leptospira motility2017

    • Author(s)
      Takabe Kyosuke、Kawamoto Akihiro、Tahara Hajime、Kudo Seishi、Nakamura Shuichi
    • Journal Title

      Biochemical and biophysical research communications

      Volume: 491 Issue: 4 Pages: 1040-1046

    • DOI

      10.1016/j.bbrc.2017.08.007

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The role of a cytoplasmic loop of MotA in load-dependent assembly and disassembly dynamics of the MotA/B stator complex in the bacterial flagellar motor.2017

    • Author(s)
      Pourjaberi, S.N.S., Terahara, N., Namba, K. & Minamino, T.
    • Journal Title

      Mol. Microbiol.

      Volume: 106 Issue: 4 Pages: 646-658

    • DOI

      10.1111/mmi.13843

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Na+-induced structural transition of MotPS for stator assembly of the Bacillus flagellar motor2017

    • Author(s)
      Terahara Naoya、Kodera Noriyuki、Uchihashi Takayuki、Ando Toshio、Namba Keiichi、Minamino Tohru
    • Journal Title

      Science Advances

      Volume: 3 Issue: 11 Pages: 4119-4119

    • DOI

      10.1126/sciadv.aao4119

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural differences in the bacterial flagellar motor among bacterial species2017

    • Author(s)
      Terashima Hiroyuki、Kawamoto Akihiro、Morimoto Yusuke V.、Imada Katsumi、Minamino Tohru
    • Journal Title

      Biophysics and Physicobiology

      Volume: 14 Issue: 0 Pages: 191-198

    • DOI

      10.2142/biophysico.14.0_191

    • NAID

      130006252950

    • ISSN
      2189-4779
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of a clockwise-Iocked deletion in FliG on the FliG ring structure of the bacterial flagellar motor.2017

    • Author(s)
      Kinoshita, M., Namba, K. & Minamino, T.
    • Journal Title

      Genes to Cells

      Volume: 23 Issue: 3 Pages: 241-247

    • DOI

      10.1111/gtc.12565

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Determination of local pH differences within living Salmonella cells by high-resolution pH imaging based on pH-sensitive GFP derivative, pHluorin(M153R)2017

    • Author(s)
      Yusuke V. Morimoto, Nobunori Kami-ike, Keiichi Namba, Tohru Minamino
    • Journal Title

      Bio-protocol

      Volume: 7 Issue: 1

    • DOI

      10.21769/bioprotoc.2529

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure of actomyosin rigour complex at 5.2Å resolution and insights into the ATPase cycle mechanism.2017

    • Author(s)
      Fujii, T. & Namba, K.
    • Journal Title

      Nat. Commun.

      Volume: 8 Issue: 1 Pages: 13969-13969

    • DOI

      10.1038/ncomms13969

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identical folds used for distinct mechanical functions of the bacterial flagellar rod and hook.2017

    • Author(s)
      Fujii, T., Kato, T., Hiraoka, K.D., Miyata, T., Minamino, T., Chevance, F., Hughes, K. & Namba, K.
    • Journal Title

      Nat. Commun.

      Volume: 8 Issue: 1 Pages: 14276-14276

    • DOI

      10.1038/ncomms14276

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Bacterial flagella grow through an injection-diffusion mechanism.2017

    • Author(s)
      Renault, T.T., Abraham, A.O., Bergmiller, T., Parados, G., Rainville, S., Charpentier, E., Guet, C.C., Tu, Y., Namba, K., Keener, J.P., Minamino, T., Erhardt, M.
    • Journal Title

      eLife

      Volume: 6

    • DOI

      10.7554/elife.23136

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Load- and polysaccharide-dependent activation of the Na+-type MotPS stator in the Bacillus subtilis flagellar motor.2017

    • Author(s)
      Terahara, N., Noguchi, Y., Nakamura, S., Kamiike, N., Ito, M., Namba, K., Minamino, T.
    • Journal Title

      Sci. Rep.

      Volume: 7 Issue: 1 Pages: 46081-46081

    • DOI

      10.1038/srep46081

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Straight and rigid flagellar hook made by insertion of the FlgG specific sequence into FlgE2017

    • Author(s)
      Koichi D. Hiraoka, Yusuke V. Morimoto, Yumi Inoue, Takashi Fujii, Tomoko Miyata, Fumiaki Makino, Tohru Minamino, Keiichi Namba
    • Journal Title

      Scientific reports

      Volume: 7 Issue: 1 Pages: 46723-46723

    • DOI

      10.1038/srep46723

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bacterial intracellular sodium ion measurement using CoroNa Green2017

    • Author(s)
      Yusuke V. Morimoto, Keiichi Namba, Tohru Minamino
    • Journal Title

      Bio-protocol

      Volume: 7 Issue: 1

    • DOI

      10.21769/bioprotoc.2092

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Measurements of free-swimming speed of motile Salmonella cells in liquid media2017

    • Author(s)
      Yusuke V. Morimoto, Keiichi Namba, Tohru Minamino
    • Journal Title

      Bio-protocol

      Volume: 7 Issue: 1

    • DOI

      10.21769/bioprotoc.2093

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Periodicity in attachment organelle revealed by electron cryotomography suggests conformational changes in gliding mechanism of Mycoplasma pneumoniae.2016

    • Author(s)
      Kawamoto, A., Matsuo, L., Yamamoto, H., Namba, K., Miyata, M.
    • Journal Title

      mBio

      Volume: 7(2) Issue: 2

    • DOI

      10.1128/mbio.00243-16

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rearrangements of α-helical structures of FlgN chaperone control the binding affinity for its cognate substrates during flagellar type III export.2016

    • Author(s)
      Kinoshita, M., Nakanishi, Y., Furukawa, Y., Namba, K., Imada, K., Minamino, T.
    • Journal Title

      Mol. Microbiol.

      Volume: 101 Issue: 4 Pages: 656-670

    • DOI

      10.1111/mmi.13415

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Structural flexibility of the periplasmic protein, FlgA, regulates flagellar P-ring assembly in Salmonella enterica.2016

    • Author(s)
      Matsunami, H., Yoon, Y.-H., Meshcheryakov, V. A., Namba, K. & Samatey, F. A.
    • Journal Title

      Sci. Rep.

      Volume: 6 Pages: 27399-27399

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural study of MPN387, an essential protein for gliding motility of a human pathogenic bacterium, Mycoplasma pneumoniae2016

    • Author(s)
      Kawakita Y, Kinoshita M, Furukawa Y, Tulum I, Tahara YO, Katayama E, Namba K, Miyata M
    • Journal Title

      Journal of Bacteriology

      Volume: 198 Issue: 17 Pages: 2352-9

    • DOI

      10.1128/jb.00160-16

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The tetrameric MotA complex as the core of the flagellar motor stator from hyperthermophilic bacterium2016

    • Author(s)
      Takekawa N, Terahara N, Kato T, Gohara M, Mayanagi K, Hijikata A, Onoue Y, Kojima S, Shirai T, Namba K, Homma M
    • Journal Title

      Sci Rep

      Volume: 6 Issue: 1 Pages: 31526-31526

    • DOI

      10.1038/srep31526

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural stability of flagellin subunit affects the rate of flagellin export in the absence of FliS chaperone.2016

    • Author(s)
      Furukawa, Y., Inoue, Y., Sakaguchi, A., Mori, Y., Fukumura, T., Miyata, T., Namba, K., Minamino, T.
    • Journal Title

      Mol. Microbiol.

      Volume: 102 Issue: 3 Pages: 405-416

    • DOI

      10.1111/mmi.13469

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The architecture of the cytoplasmic region of type Ⅲ secretion systems.2016

    • Author(s)
      Makino, F., Shen, D., Kajimura, N., Kawamoto, A., Pissaridou, P., Oswin, H., Pain M., Murillo, I., Namba, K. & Blocker, A. J.
    • Journal Title

      Sci. Rep.

      Volume: 6 Issue: 1 Pages: 33341-33341

    • DOI

      10.1038/srep33341

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] High-resolution pH imaging of living bacterial cell to detect local pH differences2016

    • Author(s)
      Yusuke V. Morimoto, Nobunori Kami-ike, Tomoko Miyata, Akihiro Kawamoto, Takayuki Kato, Keiichi Namba, Tohru Minamino
    • Journal Title

      mBio

      Volume: 7 Issue: 6

    • DOI

      10.1128/mbio.01911-16

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Genetic analysis of revertants isolated from the rod-fragile <i>fliF</i> mutant of <i>Salmonella</i>2016

    • Author(s)
      Hitomi Komatsu, Fumio Hayashi, Masahiro Sasa, Koji Shikata, Shigeru Yamaguchi, Keiichi Namba and Kenji Oosawa
    • Journal Title

      Biophysics and Physicobiology

      Volume: 13 Issue: 0 Pages: 13-25

    • DOI

      10.2142/biophysico.13.0_13

    • NAID

      130005123830

    • ISSN
      2189-4779
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The bacterial flagellar type III export gate complex is a dual fuel engine that can use both H+ and Na+ for flagellar protein export2016

    • Author(s)
      Minamino T, Morimoto YV, Hara N, Aldridge PD, Namba K
    • Journal Title

      PLoS Pathog

      Volume: 12 Issue: 3 Pages: e1005495-e1005495

    • DOI

      10.1371/journal.ppat.1005495

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Domain-swap polymerization drives the self-assembly of the bacterial flagellar motor2016

    • Author(s)
      Matthew A.B. Baker, Robert M.G. Hynson, Lorraine A. Ganuelas, Nasim Shah Mohammadi, Chu Wai Liew, Anthony A. Rey, Anthony P. Duff, Andrew E. Whitten, Cy M. Jeffries, Nicolas J. Delalez, Yusuke V. Morimoto, Daniela Stock, Judith P. Armitage, Andrew J. Turberfield, Keiichi Namba, Richard M. Berry and Lawrence K. Lee
    • Journal Title

      Nature Structural & Molecular Biology

      Volume: 23 Issue: 3 Pages: 197-203

    • DOI

      10.1038/nsmb.3172

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Insight into the flagella type III export revealed by the complex structure of the type III ATPase and its regulator.2016

    • Author(s)
      Imada, K., Minamino, T., Uchida, Y., Kinoshita, M., Namba, K.
    • Journal Title

      Proc. Natl Acad. Sci. USA

      Volume: 113 Issue: 13 Pages: 3633-3638

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Substrate-dependent dynamics of the multidrug efflux transporter AcrB of Escherichia coli2016

    • Author(s)
      Yamamoto, K., Tamai, R., Yamazaki, M., Inaba, T., Sowa, Y. & Kawagishi, I
    • Journal Title

      Sci Rep

      Volume: 6 Issue: 1 Pages: 21909-21909

    • DOI

      10.1038/srep21909

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of a Vibrio cholerae chemoreceptor that senses taurine and amino acids as attractants.2016

    • Author(s)
      Nishiyama S, Takahashi Y, Yamamoto K, Suzuki D, Itoh Y, Sumita K, Uchida Y, Homma M, Imada K, Kawagishi I.
    • Journal Title

      Sci Rep.

      Volume: 6 Issue: 1 Pages: 20866-20866

    • DOI

      10.1038/srep20866

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] FliH and FliI ensure efficient energy coupling of flagellar type III protein export in Salmonella.2016

    • Author(s)
      Minamino T, Kinoshita M, Inoue Y, Morimoto YV, Ihara K, Koya S, Hara N, Nishioka N, Kojima S, Homma M, Namba K
    • Journal Title

      Microbiologyopen

      Volume: - Issue: 3 Pages: 340-340

    • DOI

      10.1002/mbo3.340

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structure of the complex of F-actin and DNGR-1, a C-type lectin receptor involved in dendritic cell cross-presentation of dead cell-associated antigens.2015

    • Author(s)
      Hanč, P., Fujii, T., Iborra, S., Yamada, Y., Huotari, J., Schulz, O., Ahrens, S., Kjær, S., Way, M., Sancho, D., Namba, K., Reis e Sousa, C.
    • Journal Title

      Immunity

      Volume: 42 Issue: 4 Pages: 839-849

    • DOI

      10.1016/j.immuni.2012.03.008

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A repeat unit of Vibrio diarrheal T3S effector subverts cytoskeletal actin homeostasis via binding to interstrand region of actin filaments.2015

    • Author(s)
      Nishimura M, Fujii T, Hiyoshi H, Makino F, Inoue H, Motooka D, Kodama T, Ohkubo T, Kobayashi Y, Nakamura S, Namba K, Iida T.
    • Journal Title

      Sci Rep.

      Volume: 5 Issue: 1 Pages: 10870-10870

    • DOI

      10.1038/srep10870

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cryogenic coherent x-ray diffraction imaging for biological non-crystalline particles using the KOTOBUKI-1 diffraction apparatus at SACLA2015

    • Author(s)
      Tomotaka Oroguchi, Yuki Sekiguchi, Amane Kobayashi,Yu Masaki, Asahi Fukuda, Saki Hashimoto, Masayoshi Nakasako, Yuichi Ichikawa, Hitoshi Kurumizaka, Mitsuhiro Shimizu et al.,
    • Journal Title

      Journal of Physics B: Atomic, Molecular and Optical Physics

      Volume: 48 Issue: 18 Pages: 184003-184003

    • DOI

      10.1088/0953-4075/48/18/184003

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Weak interactions between Salmonella enterica FlhB and other flagellar export apparatus proteins govern type III secretion dynamics2015

    • Author(s)
      McMurry, J.L., Minamino, T., Furukawa, Y., Francis, J.W., Hill, S.A., Helms, K.A., Namba, K.
    • Journal Title

      PLOS One

      Volume: 10 Issue: 8 Pages: e0134884-e0134884

    • DOI

      10.1371/journal.pone.0134884

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Three-dimensional reconstruction from electron microscopy images combined with cysteine-mediated crosslinking provide a model of T3SS needle tip complex.2015

    • Author(s)
      Cheung, M.
    • Journal Title

      Mol. Microbiol.

      Volume: 95 Issue: 1 Pages: 31-50

    • DOI

      10.1111/mmi.12843

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The unexpected role of polyubiquitin chains in the formation of fibrillar aggregates.2015

    • Author(s)
      Morimoto D,Walinda E, Fukada H, Sou YS, Kageyama S, Hoshino M, Fujii T, Tsuchiya H, Saeki Y, Arita K, Ariyoshi M, Tochio H, Iwai K, Namba K, Komatsu M, Tanaka K, Shirakawa M.
    • Journal Title

      Nat Commun.

      Volume: 6 Issue: 1 Pages: 6116-6116

    • DOI

      10.1038/ncomms7116

    • NAID

      120005528125

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Importance of the monomer-dimer-tetramer interconversion of the clock protein KaiB in the generation of circadian oscillations in cyanobacteria2015

    • Author(s)
      Iida T, Mutoh, R, Onai, K, Morishita, M, Furukawa, Y, Namba, K, Ishiura, M
    • Journal Title

      Genes Cells

      Volume: 20 Issue: 3 Pages: 173-90

    • DOI

      10.1111/gtc.12211

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hypoxia-induced localization of chemotaxis-related signaling proteins in Vibrio cholerae.2015

    • Author(s)
      Hiremath G, Hyakutake A, Yamamoto K, Ebisawa T, Nakamura T, Nishiyama S, Homma M, Kawagishi I.
    • Journal Title

      Mol Microbiol

      Volume: 5 Issue: 5 Pages: 780-790

    • DOI

      10.1111/mmi.12887

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Liquid-based iterative recombineering method tolerant to counter-selection escapes.2015

    • Author(s)
      Tominaga M, Kawai-Noma S, Kawagishi I, Sowa Y, Saito K, Umeno D.
    • Journal Title

      PLoS One.

      Volume: 5 Issue: 3 Pages: e0119818-e0119818

    • DOI

      10.1371/journal.pone.0119818

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A putative porin gene of Burkholderia sp. NK8 involved in chemotaxis toward2015

    • Author(s)
      Yamamoto-Tamura K, Kawagishi I, Ogawa N, Fujii T.
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 79 Issue: 6 Pages: 926-936

    • DOI

      10.1080/09168451.2015.1006571

    • NAID

      120005606588

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of the MotB(D33N)mutation on stator assembly and rotation of the proton-driven bacterial flagellar motor2014

    • Author(s)
      Nakamura, S., Minamino, T., Kami-ike, N., Kudo, S. & Namba, K.
    • Journal Title

      BIOPHYSICS

      Volume: 10 Pages: 35-41

    • NAID

      130004940801

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of the periplasmic domain of FliP, an integral membrane component of the bacterial flagellar type III protein-export apparatus2014

    • Author(s)
      Fukumura T, Furukawa Y, Kawaguchi T, Saijo-Hamano Y, Namba K, Imada K, Minamino T
    • Journal Title

      Acta Crystallogr F Struct Biol Commun.

      Volume: 70(Pt 9) Issue: 9 Pages: 1215-1218

    • DOI

      10.1107/s2053230x14014678

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Assembly dynamics and the roles of FliI ATPase of the bacterial flagellar export apparatus.2014

    • Author(s)
      Bai, F.
    • Journal Title

      Sci. Rep.

      Volume: 4 Issue: 1 Pages: 6528-6528

    • DOI

      10.1038/srep06528

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The bacterial flagellar protein export apparatus processively transports flagellar proteins even with extremely infrequent ATP hydrolysis.2014

    • Author(s)
      Minamino, T.
    • Journal Title

      Sci. Rep.

      Volume: 4 Issue: 1 Pages: 7579-7579

    • DOI

      10.1038/srep07579

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Swimming behaviour and magnetotaxis function of the marine bacterium strain MO-1.2014

    • Author(s)
      Zhang, S.-D., Petersen, N., Zhang, W.-J., Cargou, S., Ruan, J., Murat, D., Santini, C.-L., Song, T., Kato, T., Notareschi, P., Li, Y., Namba, K., Gue, An-M. & Wu,L.-F.
    • Journal Title

      Environmental Microbiology Reports

      Volume: 6 Issue: 1 Pages: 14-20

    • DOI

      10.1111/1758-2229.12102

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Load-sensitive coupling of proton translocation and torque generation in the bacterial flagellar motor.2014

    • Author(s)
      Che Y-S, Nakamura S, Morimoto YV, Kami-ike N, Namba K, Minamino T.
    • Journal Title

      Mol. Microbiol.

      Volume: 91 Issue: 1 Pages: 175-184

    • DOI

      10.1111/mmi.12453

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assembly and stoichiometry of FliF and FlhA in Salmonella flagellar basal body.2014

    • Author(s)
      Morimoto YV, Ito M, Hiraoka KD, Che Y-S, Bai F, Kami-ike N, Namba K, Minamino T.
    • Journal Title

      Mol. Microbiol.

      Volume: 91 Issue: 6 Pages: 1214-1226

    • DOI

      10.1111/mmi.12529

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression, purification, crystallization and preliminary X-ray diffraction analysis of a core fragment of FlgG, a bacterial flagellar rod protein.2013

    • Author(s)
      Saijo-Hamano, Y., et al
    • Journal Title

      Acta Cryst.

      Volume: F69 Issue: 5 Pages: 547-550

    • DOI

      10.1107/s1744309113007392

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Common evolutionary origin for the rotor domain of rotary ATPases and Flagellar protein export apparatus.2013

    • Author(s)
      Kishikawa, J., et al
    • Journal Title

      PLoS One

      Volume: 8 Issue: 5 Pages: 64692013-64692013

    • DOI

      10.1371/journal.pone.0064695

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of the Dc domain of the bacterial hook protein FlgE in hook assembly and function2013

    • Author(s)
      Moriya, N., Minamino, T., Ferris, U. H., Morimoto., V. Y., Ashihara, M., Kato, T. Namba, K.
    • Journal Title

      BIOPHYSICS

      Volume: 9 Issue: 0 Pages: 63-72

    • DOI

      10.2142/biophysics.9.63

    • NAID

      130003366031

    • ISSN
      1349-2942
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure of the ParM filament at 8.5Å resolution.2013

    • Author(s)
      Gayathri, P., Fujii, T., Namba, K. Lowe, J.
    • Journal Title

      J. Struct. Biol.

      Volume: 184 Pages: 33-42

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Populational Heterogeneity vs. Temporal Fluctuation in Escherichia coli Flagellar Motor Switching.2013

    • Author(s)
      Bai, F., Che, Y-S., Kami-ike, N., Ma, Q., Minamino, T., Sowa, Y. & Namba, K.
    • Journal Title

      Biophys. J.

      Volume: 105 Issue: 9 Pages: 2123-2129

    • DOI

      10.1016/j.bpj.2013.09.043

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Correlation between supercoiling and conformational motions of the bacterial flagellar filament.2013

    • Author(s)
      Stadler, A. M., Unruh, T., Namba, K., Samatey, F., Zaccai, G.
    • Journal Title

      Biophysical Journal

      Volume: 105 Issue: 9 Pages: 2157-2165

    • DOI

      10.1016/j.bpj.2013.09.039

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interactions of bacterial flagellar chaperone-substrate complexes with FlhA contribute to coordinating assembly of the flagellar filament.2013

    • Author(s)
      Kinoshita M, Hara N, Imada K, Namba K, Minamino T.
    • Journal Title

      Mol. Microbiol.

      Volume: 90 Issue: 6 Pages: 1249-1261

    • DOI

      10.1111/mmi.12430

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Common and distinct structural features of Salmonella injectisome and flagellar basal body2013

    • Author(s)
      Kawamoto, A., Morimoto, Y.V., Miyata, T., Minamino, T., Hughes, K.T., Kato, T. & Namba, K.
    • Journal Title

      Scientific Reports

      Volume: 3 Issue: 1 Pages: 3369-3369

    • DOI

      10.1038/srep03369

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The C-terminal periplasmic domain of MotB is responsible for load-dependent control of the number of stators of the bacterial flagellar motor2013

    • Author(s)
      D. J. Castillo1, S. Nakamura, Y. V. Morimoto, Y.-S. Che, N. Kami-ike1, S. Kudo, T. Minamino, K. Namba
    • Journal Title

      BIOPHYSICS

      Volume: 9 Issue: 0 Pages: 173-181

    • DOI

      10.2142/biophysics.9.173

    • NAID

      130003383787

    • ISSN
      1349-2942
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] クライオ電子顕微鏡による生体分子立体構造解析法の原子分解能をめざした技術開発.2019

    • Author(s)
      難波啓一
    • Organizer
      大阪大学先導的学際研究機構創薬サイエンス部門・生命医科学融合フロンティア研究部門合同シンポジウム「未来医療を推進する革新的技術のフロンティア」
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Salmonella typhimuriumべん毛たんぱく質FljBの構造とその特徴2019

    • Author(s)
      山口智子, 寺原直矢, 加藤貴之, 當間頌子, 宮田知子, 難波啓一.
    • Organizer
      2019年生体運動研究合同班会議
    • Related Report
      2018 Annual Research Report
  • [Presentation] べん毛モーター固定子のイオン透過性を向上させる回転子FliGの変異2019

    • Author(s)
      寺原直矢, 難波啓一, 南野徹.
    • Organizer
      2019年生体運動研究合同班会議
    • Related Report
      2018 Annual Research Report
  • [Presentation] サルモネラフックの“自然”な構造の解析.2019

    • Author(s)
      加藤貴之, 宮田知子, Peter Horvath, 難波啓一
    • Organizer
      2018年度べん毛研究交流会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細菌べん毛モーターの回転方向スイッチ機構の解明.2019

    • Author(s)
      宮田知子, 加藤貴之, 川本晃大, 牧野文信, 難波啓一.
    • Organizer
      2018年度べん毛研究交流会
    • Related Report
      2018 Annual Research Report
  • [Presentation] SalmonellaのFljBべん毛繊維とFliCべん毛繊維の比較.2019

    • Author(s)
      山口智子, 加藤貴之, 當間頌子, 宮田知子, 難波啓一
    • Organizer
      2018年度べん毛研究交流会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 固定子複合体のペリプラズミックドメインにおけるイオン選択性.2019

    • Author(s)
      寺原直矢, 小寺哲幸, 安藤敏夫, 難波啓一, 南野徹
    • Organizer
      2018年度べん毛研究交流会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Recent progress and future of electron cryomicroscopy for the structural analysis of protein assemblies at the atomic level.2018

    • Author(s)
      Namba, K.
    • Organizer
      Lectureship in Biological Sciences, Texas A&M University
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Structure and dynamics of macromolecular motors revealed by electron cryomicroscopy and optical nanophotometry.2018

    • Author(s)
      Namba, K.
    • Organizer
      Lectureship in Biological Sciences, Texas A&M University
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Assembly and activation mechanism of the transmembrane stator complex in the bacterial flagellar motor.2018

    • Author(s)
      Minamino, T.
    • Organizer
      The 23rd Biophysics Conference, National Chung Hsing University
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 新型クライオ電子顕微鏡“CryoARM”の開発.2018

    • Author(s)
      加藤貴之, 難波啓一
    • Organizer
      日本顕微鏡学会第74回学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] クライオ電子顕微鏡で解き明かす細菌べん毛モーター回転子の立体構造と回転対象性.2018

    • Author(s)
      川本晃大, 宮田知子, 木下実紀, 南野徹, 加藤貴之, 難波啓一.
    • Organizer
      日本顕微鏡学会第74回学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] クライオ電子顕微鏡による構造解析法の原子分解能をめざした技術開発.2018

    • Author(s)
      難波啓一.
    • Organizer
      原子・分子・光科学(AMO)討論会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 生体分子の原子レベルの立体構造解析法として進歩したクライオ電子顕微鏡法の最近の動向.2018

    • Author(s)
      難波啓一.
    • Organizer
      第18回日本蛋白質科学会年会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] クライオ電子顕微鏡を用いたグルタミン酸脱水素酵素ドメイン運動の解明.2018

    • Author(s)
      大出真央, 加藤貴之, 笹口友隆, 難波啓一, 中迫雅由
    • Organizer
      第18回日本蛋白質科学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] べん毛先端のキャップ構造.2018

    • Author(s)
      牧野文信, 木田葵, 木下実紀, 宮田知子, 加藤貴之, 南野徹, 難波啓一, 今田勝巳
    • Organizer
      第18回日本蛋白質科学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] クライオ電子顕微鏡で解き明かす細菌べん毛モーター回転子の立体構造と回転対象性.2018

    • Author(s)
      川本晃大, 宮田知子, 木下実紀, 南野徹, 加藤貴之, 難波啓一.
    • Organizer
      第18回日本蛋白質科学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Recent progress and future of electron cryomicroscopy for the structural analysis of protein assemblies at the atomic level.2018

    • Author(s)
      Namba, K.
    • Organizer
      2018 Kuo Symposium on 3D-EM of macromolecules and cells
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 生体超分子モーターの高効率なエネルギー変換のメカニズム2018

    • Author(s)
      難波啓一
    • Organizer
      生物物理若手の会第58回夏の学校
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Recent progress and future of electron cryomicroscopy f or the structural analysis of protein assemblies at the atomic level.2018

    • Author(s)
      Namba, K.
    • Organizer
      Academia Sinica Cryo-EM Grand Opening Symposium & Workshop
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 生体分子の原子レベルの立体構造解析法として進歩したクライオ電子顕微鏡法の最近の動向2018

    • Author(s)
      難波啓一
    • Organizer
      JASIS2018ライフサイエンスイノベーション
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] On the fly analysis for screening thin ice samples.2018

    • Author(s)
      Kato, T.
    • Organizer
      Academia Sinica Cryo-EM Grand Opening Symposium & Workshop
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Structures and dynamics of skeletal muscle actomyosin and bacterial flagellar motor.2018

    • Author(s)
      Namba, K.
    • Organizer
      Scottish Centre for Macromolecular Imaging Opening Symposium Cryogenic-Electron Microscopy-Seeing the Machinery of Life
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Common features and mechanisms of skeletal muscle actomyosin and bacterial flagellar motor revealed by electron cryomicroscopy and optical nanophotometry.2018

    • Author(s)
      Namba, K.
    • Organizer
      Seminar, MRC-LMB
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Recent development and its future potential of a JEOL electron cryomicroscope for the structural analysis of protein assemblies at atomic level.2018

    • Author(s)
      Namba, K.
    • Organizer
      Seminar, MRC-LMB
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 最新クライオ電子顕微鏡による構造解析2018

    • Author(s)
      加藤貴之
    • Organizer
      第79回応用物理学会秋季学術講演会分科企画シンポジウム“クライオ電子顕微鏡最前線”
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] バクテリアべん毛モーターの固定子再編成における自律的制御.2018

    • Author(s)
      南野徹
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細菌べん毛モーターの回転速度と構成ユニット数の関係.2018

    • Author(s)
      石田翼, 吉多美祐, 南野徹, 曽和義幸.
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Gwatch : クライオ電顕におけるハイスループット評価システムの構築と評価.2018

    • Author(s)
      牧野文信, 加藤貴之, 深川竜郎, 難波啓一
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細菌べん毛繊維の成長端の構造.2018

    • Author(s)
      木田 葵, 牧野文信, 木下実紀, 宮田知子, 加藤貴之, 南野徹, 難波啓一, 今田勝巳.
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] クライオ電子顕微鏡で解き明かす細菌べん毛モーターのトルク伝達に重要な回転対称構造.2018

    • Author(s)
      川本晃大, 宮田知子, 木下実紀, 南野徹, 今田勝巳, 加藤貴之, 難波啓一.
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] クライオ電子顕微鏡における高分解能構造解析のためのスクリーニング法の検討.2018

    • Author(s)
      加藤貴之, 寺原直矢, 宮田知子, 難波啓一.
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Effect of pH on rotation of the proton-driven bacterial flagellar motor under near zero load.2018

    • Author(s)
      花泉裕大, 中村修一, 森本雄祐, 南野徹, 難波啓一.
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] サルモネラ菌べん毛タンパク質FliCとFljBで構成された繊維構造の比較と機能の違い.2018

    • Author(s)
      山口智子, 加藤貴之, 寺原直矢, 當間頌子, 難波啓一.
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] クライオ電子顕微鏡によるグルタミン酸脱水素酵素ドメイン運動の可視化.2018

    • Author(s)
      大出真央, 加藤貴之, 苙口友隆, 難波啓一, 中迫雅由.
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細菌べん毛モーターの回転方向変換制御機構の解明.2018

    • Author(s)
      宮田知子, 加藤貴之, 川本晃大, 牧野文信, 難波啓一.
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Assignment of subunit components in motor evolved from F-ATPase for Mycoplasma mobile gliding.2018

    • Author(s)
      豊永拓真, 加藤貴之, 川本晃大, 古寺哲幸, 安藤敏夫, 難波啓一, 宮田真人.
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Feedback regulation of the ion channel activity of the flagellar motor stator complex.2018

    • Author(s)
      寺原直矢, 難波啓一, 南野徹.
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] バクテリアべん毛輸送ゲート複合体の構造機能解析.2018

    • Author(s)
      木下実紀, 宮田知子, 川本晃大, 加藤貴之, 難波啓一, 南野徹.
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Structure and Dynamics of Macromolecular Motors Revealed by Electron Cryomicroscopy and Optical Nanophotometry.2018

    • Author(s)
      Namba, K.
    • Organizer
      6th International Life-Science Symposium for young scientist
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 生体超分子モーターの高効率なエネルギー変換メカニズム2018

    • Author(s)
      難波啓一
    • Organizer
      2018年度東京大学生物生産工学研究センターシンポジウム「タンパク質の構造・機能研究の最先端」
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 超分子モーターの構造と高効率なエネルギー変換機構2018

    • Author(s)
      難波啓一
    • Organizer
      片岡幹雄先生ご退職記念研究会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 生体分子ナノマシンの立体構造解析に原子レベルで迫る!2018

    • Author(s)
      難波啓一
    • Organizer
      第18回「大学リレーセミナー」
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] バクテリアべん毛輸送ゲート複合体の膜電位依存的活性化機構2018

    • Author(s)
      南野徹
    • Organizer
      日本生体エネルギー研究会第44回討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] べん毛モーターの回転速度が「速くなった」変異株の単離と回転計測によるモーター特性の解析2018

    • Author(s)
      寺原直矢, 難波啓一, 南野徹.
    • Organizer
      日本生体エネルギー研究会第44回討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Insights into the mechamism of bacterial flagellar motor rotation from the structure and dynamics.2018

    • Author(s)
      Namba, K.
    • Organizer
      GRC on Sensory Transduction in Microorganisms Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Structures and dynamics of macromolecular motors revealed by electron cryomicroscopy and optical nanophotometry.2018

    • Author(s)
      Namba, K.
    • Organizer
      NanoLSI第1回国際シンポジウム
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 細菌べん毛基部体の極低温電子顕微鏡による構造解析.2018

    • Author(s)
      宮田知子, 加藤貴之, 牧野文信, 川本晃大, 西條(濱野)由見子, 難波啓一
    • Organizer
      第23回べん毛研究交流会
    • Related Report
      2017 Annual Research Report
  • [Presentation] べん毛先端のキャップ構造.2018

    • Author(s)
      木田葵, 牧野文信, 木下実紀, 宮田知子, 加藤貴之, 南野徹, 難波啓一, 今田勝巳.
    • Organizer
      第23回べん毛研究交流会
    • Related Report
      2017 Annual Research Report
  • [Presentation] FliF-G融合変異型べん毛モーターの機能解析.2018

    • Author(s)
      酒井智史, 森広一郎, 井上由美, 宮田知子, 寺原直矢, 森本雄祐, 加藤貴之, 難波啓一, 南野徹.
    • Organizer
      第23回べん毛研究交流会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 低負荷条件下で回転するべん毛モーターの計測.2018

    • Author(s)
      石田翼, 吉多美祐, 南野徹, 曽和義幸.
    • Organizer
      第23回べん毛研究交流会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 超低負荷条件下でのべん毛モーター回転計測.2018

    • Author(s)
      花泉裕大, 中村修一, 森本雄祐, 南野徹, 難波啓一.
    • Organizer
      第23回べん毛研究交流会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 枯草菌べん毛モーターの回転ステップの解析.2018

    • Author(s)
      寺原直矢, 楊未来, 難波啓一, 南野徹.
    • Organizer
      第23回べん毛研究交流会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 細菌べん毛FlgGの全長構造とディスタルロッドの構造.2018

    • Author(s)
      西條由見子, 宮田知子, 松波秀行, 難波啓一, 今田勝巳.
    • Organizer
      第23回べん毛研究交流会
    • Related Report
      2017 Annual Research Report
  • [Presentation] High resolution structural analysis of the flagellar hook of Salmonella enterica serovar Typhimurium.2018

    • Author(s)
      Horvath, P., Kato, T., Miyata, T. & Namba, K.
    • Organizer
      第23回べん毛研究交流会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 磁性細菌のべん毛運動を可視化する.2018

    • Author(s)
      下権谷祐児, 難波啓一, 石川拓司, 西山雅祥.
    • Organizer
      第23回べん毛研究交流会
    • Related Report
      2017 Annual Research Report
  • [Presentation] べん毛フィラメントキャップの構造.2018

    • Author(s)
      木田葵、牧野文信、木下実紀、宮田知子、加藤貴之、難波啓一、南野徹、今田勝巳.
    • Organizer
      第5回生命分子科学研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 反転膜小胞を用いた細菌べん毛軸構造の in vitro での構築.2018

    • Author(s)
      寺島浩行, 巽千夏, 川本晃大, 南野徹, 今田勝巳.
    • Organizer
      第91回日本細菌学会総会
    • Description
      (口頭)
    • Related Report
      2017 Annual Research Report
  • [Presentation] Motor evolved from F-ATPase for Mycoplasma mobile Gliding.2018

    • Author(s)
      豊永拓真, 加藤貴之, 川本晃大, 古寺哲幸, 安藤敏夫, 難波啓一, 宮田真人.
    • Organizer
      第91回日本細菌学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] べん毛Ⅲ型輸送装置構成蛋白質FliJとⅢ型輸送シャペロンとの相互作用(Role of interactions of FliJ with FlgN and FliT chaperones in flagellar type Ⅲ protein export)2017

    • Author(s)
      南野徹, 木下実紀, 森本雄祐, 難波啓一
    • Organizer
      第90回日本細菌学会総会
    • Place of Presentation
      仙台国際センター展示棟(宮城県仙台市)
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] 生体超分子モーターの高効率なエネルギー変換のメカニズム2017

    • Author(s)
      難波啓一
    • Organizer
      日本物理学会第72回年次大会
    • Place of Presentation
      大阪大学(大阪府豊中市)
    • Year and Date
      2017-03-18
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Insights into the mechanism of bacterial flagella motor rotation from the structure and dynamics.2017

    • Author(s)
      Namba, K.
    • Organizer
      OIST Workshop “Bacterial flagella, injectisomes & type Ⅲ secretion systems” joint with Annual Flagella Meeting
    • Place of Presentation
      OIST(沖縄県国頭郡)
    • Year and Date
      2017-03-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Visualization of 11-and 34-fold rotational symmetries in the MS ring of the bacterial flagellum by electron cryomicroscopy.2017

    • Author(s)
      Kawamoto, A., Kaido, A., Kinoshita, M., Miyata, T., Minamino, T., Kato, T., Namba, K.
    • Organizer
      OIST Workshop “Bacterial flagella, injectisomes & type Ⅲ secretion systems” joint with Annual Flagella Meeting
    • Place of Presentation
      OIST(沖縄県国頭郡)
    • Year and Date
      2017-03-02
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Elucidation of the directional switching mechanism of the bacterial flagellar motor by electron cryomicroscopy.2017

    • Author(s)
      Miyata, T., Kato, T., Kawamoto, A., Makino, F., Namba, K.
    • Organizer
      OIST Workshop “Bacterial flagella, injectisomes & type Ⅲ secretion systems” joint with Annual Flagella Meeting
    • Place of Presentation
      OIST(沖縄県国頭郡)
    • Year and Date
      2017-03-02
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The two distinct conformational states of the flagellar polyrod depending on ionic-strength revealed by CryoEM.2017

    • Author(s)
      Fujii, T., Yamada, Y., Namba, K.
    • Organizer
      OIST Workshop “Bacterial flagella, injectisomes & type Ⅲ secretion systems” joint with Annual Flagella Meeting
    • Place of Presentation
      OIST(沖縄県国頭郡)
    • Year and Date
      2017-03-02
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Conformational rearrangement of F1hA is critical for flagellar type Ⅲ protein export.2017

    • Author(s)
      Inoue, Y., Kinoshita, M., Kitano, A., Imada, K., Namba, K., Minamino, T.
    • Organizer
      OIST Workshop “Bacterial flagella, injectisomes & type Ⅲ secretion systems” joint with Annual Flagella Meeting
    • Place of Presentation
      OIST(沖縄県国頭郡)
    • Year and Date
      2017-03-01
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Role of intrinsically disordered region of the C-terminal domain of FliK in substrate specificity switching of the bacterial flagellar type Ⅲ export aparatus.2017

    • Author(s)
      Kinoshita, M., Aizawa, S., Namba, K., Minamino, T.
    • Organizer
      OIST Workshop “Bacterial flagella, injectisomes & type Ⅲ secretion systems” joint with Annual Flagella Meeting
    • Place of Presentation
      OIST(沖縄県国頭郡)
    • Year and Date
      2017-03-01
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In Vitro reconstitution of the protein export of the flagellar type Ⅲ secretion system by using the inverted membrane vesicles.2017

    • Author(s)
      Terashima, H., Kawamoto, A., Tatsumi, C., Namba, K., Minamino, T., Imada, K.
    • Organizer
      OIST Workshop “Bacterial flagella, injectisomes & type Ⅲ secretion systems” joint with Annual Flagella Meeting
    • Place of Presentation
      OIST(沖縄県国頭郡)
    • Year and Date
      2017-03-01
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] クライオ電顕で探る生体超分子モーターの高効率なエネルギー変換メカニズム2017

    • Author(s)
      難波啓一
    • Organizer
      第23回「プロテイン・モール関西」情報交流セミナー
    • Place of Presentation
      千里ライフサイエンスセンター(大阪府豊中市)
    • Year and Date
      2017-02-24
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 生体超分子モーターの高効率なエネルギー変換のメカニズム2017

    • Author(s)
      難波啓一
    • Organizer
      N-Hybrid conference 2017
    • Place of Presentation
      朱鷺メッセ 新潟コンベンションセンター(新潟県新潟市)
    • Year and Date
      2017-01-21
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] CryoEM structural analysis of muscle thin filament composed of actin filament, tropomyosin and troponin.2017

    • Author(s)
      Fujii, T., Yamada, Y., Namba, K.
    • Organizer
      JSTさきがけーライフサイエンスの革新を目指した構造生命科学と先端的基盤技術-研究領域研究成果報告会第2期生(平成25年度採択)
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2017-01-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] Structures and dynamics of macromolecular motors revealed by electron cryomicroscopy and optical nanophotometry.2017

    • Author(s)
      Namba, K.
    • Organizer
      Department Seminar
    • Place of Presentation
      Northeastern University, Boston, USA
    • Year and Date
      2017-01-10
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Structures and dynamics of TMV, actomyosin and bacterial flagellar filaments.2017

    • Author(s)
      Namba, K.
    • Organizer
      Caspar Structural Biology Symposium
    • Place of Presentation
      Florida State University, Florida, USA
    • Year and Date
      2017-01-08
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] F1-ATPaseから進化したマイコプラズマ滑走運動のモーター2017

    • Author(s)
      豊永拓真, 加藤貴之, 川本晃大, 浜口祐, 田原悠平, 難波啓一, 宮田真人
    • Organizer
      2017年生体運動研究合同班会議
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2017-01-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] クライオ電子顕微鏡で明らかにしたべん毛基部体の回転対称構造2017

    • Author(s)
      川本晃大, 海道綾菜, 木下実紀, 宮田知子, 南野徹, 加藤貴之, 難波啓一
    • Organizer
      2017年生体運動研究合同班会議
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2017-01-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] 高圧力下にある磁性細菌MO-1の運動観察2017

    • Author(s)
      西山雅祥, Juanfang Ruan, 加藤貴之, 南野徹, Long-Fei Wu, 原田慶恵
    • Organizer
      2017年生体運動研究合同班会議
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2017-01-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] High resolution structural studies and direct visual observation of Hsp 104 from Chaetomium thermophilum suggest the role of the spiral architecture.2017

    • Author(s)
      Inoue, Y., Hanazono, Y., Noi, K., Kawamoto, A., Takeda, K., Noguchi, K., Namba, K., Ogura, K., Shinohara, K. & Yohda, M.
    • Organizer
      EMBO Conference on Protein quality control : Success and failure in health and disease
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mycoplasma pneumoniaeの“あし”タンパク質・P1 adhesinの機能と構造の解明.2017

    • Author(s)
      松本優, 川本晃大, 加藤貴之, 川北祥人, 見理剛, 森茂太郎, 難波啓一, 宮田真人
    • Organizer
      日本マイコプラズマ学会第44回学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Recent progress of electron cryomicroscopy for structural analysis of protein assemblies at atomic level.2017

    • Author(s)
      Namba, K.
    • Organizer
      The 31st IFRec Colloquium
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] クライオ電子顕微鏡によるたんぱく質構造解析の現状2017

    • Author(s)
      難波啓一
    • Organizer
      第12回ソリューションサークル
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Metabolic profiling of thermos thermophiles HB8 reveals distinct metabolic states dependent on the temperature.2017

    • Author(s)
      Tokuyama, K., Sakai, T., Toya, Y., Matsuda, F., Kuramitsu, S., Shimizu, H.
    • Organizer
      IUMS 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生体超分子モーターの高効率なエネルギー変換メカニズム2017

    • Author(s)
      難波啓一
    • Organizer
      岡山大学大学院歯薬学研究科セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Structures of motor protein complexes revealed by cryoEM image analyses.2017

    • Author(s)
      Namba, K.
    • Organizer
      Recent advance in the frontiers of the cryo-EM sciences
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Mechanical communications among stator units coordinate the number of active stators in the bacterial flagellar motor.2017

    • Author(s)
      Minamino, T.
    • Organizer
      International Symposium on “Harmonized supramolecular motility machinery and its diversity”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 蛋白質膜透過駆動モーターSecDF. Protein translocation motor SecDF.2017

    • Author(s)
      Tsukazaki, T., Furukawa, A., Yoshikaie, K., Mori, T., Morimoto, Y. V., Sugano, Y., Iwaki, S., Minamino, T., Sugita, Y. & Tanaka, Y.
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Structural basis of muxcle force generation and regulatory mechanism by CryoEM.2017

    • Author(s)
      Fujii, T.
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Motor evolved from F-ATPase for Mycoplasma mobile gliding.2017

    • Author(s)
      Toyonaga, T., Kato, T., Kawamoto, Namba, K. & Miyata, M.
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] P1 adhesin, the leg for gliding of Mycoplasma pneumonia.2017

    • Author(s)
      Matsumoto, U., Kawamoto, A., Kato, T., Kawakita, Y., Kenri, T., Mori, S., Namba, K. & Miyata, M.
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Pressure-induced activation of the swimming motility of magnetotactic bacterium.2017

    • Author(s)
      Nishiyama, M., Ruan, J., Shimogonya, Y., Kato, T., Minamino, T., Namba, K., Ishikawa, T., Seiyama, A., Wu, L. -W. & Harada, Y.
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Structure and rotational symmetry of the rotor of the bacterial flagellar motor revealed by electron cryomicroscopy.2017

    • Author(s)
      Kawamoto, A., Miyata, T., Kinoshita, M., Minamino, T., Kato, T. & Namba, K.
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Towards atomic resolution structural analysis by electron cryomicroscopy.2017

    • Author(s)
      Namba, K.
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Interactions between flagellar type III export gate proteins.2017

    • Author(s)
      Kinoshita, M., Kawamoto, A., Namba, K. & Minamino, T.
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The role of a flexible linker of FlhA in substrate specificity switching of the bacterial flagellar type III export-apparatus.2017

    • Author(s)
      Inoue, Y., Kida, M., Kinoshita, M., Imada, K., Namba, K. & Minamino, T.
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Cryo-electron microscopy study toward detecting domain motion of glutamate dehydrogenase.2017

    • Author(s)
      Oide, M., Kato, T., Oroguchi, T., Namba, K. & Nakasako, M.
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Rotation assay of the proton-driven bacterial flagellar motor under near zero load.2017

    • Author(s)
      Hanaizumi, Y., Nakamura, S., Morimoto, Y. V., Minamino, T. & Namba, K.
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Measurements of motility and flagellar rotation in Psedomonas species.2017

    • Author(s)
      Hariu, T., Tensaka, T., Terahara, N., Kudo, S. & Nakamura, S.
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Real-time imaging of Na+-induced structural transitions of MotPS stator complex of flagellar motor by HS-AFM.2017

    • Author(s)
      Terahara, N., Kodera, N., Uchihashi, T., Ando, T., Namba, K. & Minamino, T.
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Structural analysis of human cariac muscle thin filaments by electron cryomicroscopy.2017

    • Author(s)
      Yamada, Y., Namba, K. & Fujii, T.
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Structural basis of the mechanical properties of the flagellar distal rod and the hook.2017

    • Author(s)
      Saijo-Hamano, Y., Matsunami, H., Namba, K. & Imada, K.
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Structural analysis of the NATIVE state flagella hook by electron cryomicroscopy.2017

    • Author(s)
      Kato, T., Miyata, T., Horvath, P. & Namba, K.
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Elucidation of the directional switching mechanism of the bacterial flagellar motor by electron cryomicroscopy.2017

    • Author(s)
      Miyata, T., Kato, T., Kawamoto, A. & Namba, K.
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] High resolution structural analysis of the flagellar hook of Salmonella Typhimurium.2017

    • Author(s)
      Horvath, P., Kato, T., Miyata, T. & Namba, K.
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 生体超分子モーターの高効率なエネルギー変換メカニズム.2017

    • Author(s)
      難波啓一
    • Organizer
      生物に学ぶ“Bioinspired systems lecture series”
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Recent progress of electron cryomicroscopy for structural analysis of protein assemblies at atomic level.2017

    • Author(s)
      Namba, K.
    • Organizer
      Seminar, National Tsing Hua University
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 細菌べん毛ⅠⅠⅠ型分泌装置によるタンパク質輸送の in vitro 再構成.2017

    • Author(s)
      寺島浩行、川本晃大、巽千夏、難波啓一、南野徹、今田勝巳.
    • Organizer
      第54回日本細菌学会中部支部総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Structures and dynamics of macromolecular motors revealed by electron cryomicroscopy and optical nanophotometry.2017

    • Author(s)
      Namba, K.
    • Organizer
      CSSB Opening Syposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Performance of the new cryo EM “cryoARM” and its structural analysis.2017

    • Author(s)
      加藤貴之
    • Organizer
      生理研研究会「クライオ電子顕微鏡によるタンパク質の高分解能単流子構造解析」
    • Related Report
      2017 Annual Research Report
  • [Presentation] 細菌べん毛モーター回転スイッチ制御機構の解明.2017

    • Author(s)
      宮田知子, 加藤貴之, 川本晃大, 難波啓一.
    • Organizer
      生理研研究会「クライオ電子顕微鏡によるタンパク質の高分解能単流子構造解析」
    • Related Report
      2017 Annual Research Report
  • [Presentation] 反転膜小胞を用いた細菌べん毛ⅠⅠⅠ型分泌装置によるタンパク質輸送のin vitro再構成.2017

    • Author(s)
      寺島浩行、川本晃大、巽千夏、難波啓一、南野徹、今田勝巳.
    • Organizer
      2017年度生命科学系学会合同年次大会(第90回日本生化学会大会)
    • Related Report
      2017 Annual Research Report
  • [Presentation] 反転膜小胞を用いた細菌べん毛軸構造のin vitroでの構築.2017

    • Author(s)
      寺島浩行、巽千夏、川本晃大、南野徹、今田勝巳.
    • Organizer
      日本生体エネルギー研究会第43回討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 蛍光ラベルした細菌べん毛蛋白質のin vitro輸送測定系を用いた検出2016

    • Author(s)
      戸野侃, 寺島浩行, 巽千夏, 川本晃大, 田端和仁, 上野博史, 松田知己, 永井健治, 野地博行, 難波啓一, 南野徹, 今田勝巳
    • Organizer
      日本生体エネルギー研究会第42回討論会
    • Place of Presentation
      名古屋工業大学(愛知県名古屋市)
    • Year and Date
      2016-12-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] 高速AFMイメージングにより明らかになったべん毛モーター固定子複合体MotPSのダイナミクス2016

    • Author(s)
      寺原直矢, 古寺哲幸, 内橋貴之, 安藤敏夫, 難波啓一, 南野徹
    • Organizer
      日本生体エネルギー研究会第42回討論会
    • Place of Presentation
      名古屋工業大学(愛知県名古屋市)
    • Year and Date
      2016-12-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] Structures and dynamics of actin and bacterial flagellar filaments.2016

    • Author(s)
      Namba, K.
    • Organizer
      IGER International Symposium on “Now in actin study : Motor protein research reaching a new stage”
    • Place of Presentation
      名古屋大学(愛知県名古屋市)
    • Year and Date
      2016-12-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] クライオ電顕単粒子解析法による赤痢菌毒素分泌装置ニードルの高分解能構造解析2016

    • Author(s)
      藤井高志
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] クライオ電子顕微鏡によるCENP-H複合体の構造解析2016

    • Author(s)
      牧野文信, マッキンリー カラ, 原昌稔, 堀哲也, 加藤貴之, チーズマン イアン, 難波啓一, 深川竜郎
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 最新低温電子顕微鏡“CryoARM”の性能. Performance of State-of-the-art Cryo EM, named “CryoARM”2016

    • Author(s)
      Kato, T., Hosogi, N., Kaneko, T., Ishikawa, I. & Namba, K.
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] Visualization of 11-and 34-fold rotational symmetries in the MS ring of the bacterial flagellum by electron cryomicroscopy.2016

    • Author(s)
      Kawamoto, A., Kaido, A., Kinoshita, M., Miyata, T., Minamino, T., Kato, T. & Namba, K.
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] 筋収縮制御メカニズムの解明を目指した細いフィラメントの立体構造解析. CryoEM structural analysis of muscle thin filament composed of actin filament, tropomyosin and troponin.2016

    • Author(s)
      Yamada, Y., Namba, K & Fujii, T.
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] サルモネラ属菌FliFG融合変異型べん毛モーターの動態機能計測. Functional analysis of a FliFG deletion-fusion mutant flagellar motor.2016

    • Author(s)
      Sakai, T., Mori, K., Inoue, Y., Miyata, T., Terahara, N., Morimoto, Y., Kato, T., Namba, K. & Minamino, T.
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] Functional analysis of slow-motile mutations in flagellar stator MotA/B of Salmonella.2016

    • Author(s)
      Shajaripourjaberi, S., Terahara, N. & Minamino, T.
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] F1hAの構造変換がべん毛蛋白質輸送順序の決定に重要である. Conformational rearrangements of F1hA is critical for ordered protein export during flagellar assembly.2016

    • Author(s)
      Inoue, Y., Ogawa, Y., Kinoshita, M., Imada, K., Namba, K. & Minamino, T.
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] 細菌べん毛Ⅲ型タンパク質輸送のin vitro再構築. In vitro reconstitution of the bacterial flagellar type Ⅲ protein export.2016

    • Author(s)
      Terashima, H., Kawamoto, A., Tatsumi, C., Namba, K., Minamino, T. & Imada, K.
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] バクテリアべん毛輸送ゲート複合体の発現系の構築と精製. Expression and purification of the bacterial flagellar type Ⅲ export gate complex.2016

    • Author(s)
      Fukumura, T., Kinoshita, M., Namba, K. & Minamino, T.
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] ロッド様に直線状で固く、野生型より長い変異型フックの金ナノ粒子標識によるべん毛モーターの高分解能回転計測. High resolution measurements of flagellar motor rotation by nanogold attached to a straight, solid hook mutant longer than the wild-type.2016

    • Author(s)
      Nakamura, S., Morimoto, Y. V., Minamino, T. & Namba, K.
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] ユビキチン化に伴う蛋白質の凝集体形成. Ubiquitylation-induced protein aggregation.2016

    • Author(s)
      Morimoto, D., Walinda, E., Fukada, H., Sugase, K., Hoshino, M., Fujii, T., Namba, K., Komatsu, M., Tanaka, K. & Shirakawa, M.
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] 赤痢菌ニードル複合体の極低温電子顕微鏡による構造解析. Structural analysis of needle complex from shigella flexneri by cryo clectron microscopy.2016

    • Author(s)
      Kajimura, N., Kato, T., Blocker, A. J. & Namba, K.
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] 反転膜小胞を用いたべん毛軸構造タンパク質よ輸送順序の解析. Secretion order of the Class-Ⅱ flagellar axial proteins analyzed by inverted membrane vesicles(IMV).2016

    • Author(s)
      Matsumoto, Y., Tatsumi, C., Terashima, H., Minamino, T. & Imada, K.
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] 細菌べん毛モーターの回転方向変換制御機構の解明. Elucidation of the directional switching mechanism of the bacterial flagellar motor by electron cryomicroscopy.2016

    • Author(s)
      Miyata, T., Kato, T., Kawamoto, A. & Namba, K.
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] マウス繊毛細胞におけるDpcdの機能解析. Role of Dpcd in motile cilia of meice.2016

    • Author(s)
      Tamegai, M., Kikumoto, M., Kinoshita, M., Kawamoto, A., Namba, K., Hamada, H., Imada, K., Narita, A. & Shinohara, K.
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] クライオ電子顕微鏡で探る生体分子モーターの構造と機能2016

    • Author(s)
      難波啓一
    • Organizer
      理研シンポジウム「クライオ電子顕微鏡が拓く未来-構造生物学研究のパラダイムシフト-」
    • Place of Presentation
      理化学研究所横浜キャンパス(神奈川県横浜市)
    • Year and Date
      2016-11-08
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Direct observation of ring formation of the C-terminal cytoplasmic domain of a flagellar type Ⅲ export gate protein FlhA by high-speed atomic force microscopy.2016

    • Author(s)
      Minamino, T., Terahara, N., Inoue, Y., Kodera, N., Ando, T. & Namba, K.
    • Organizer
      4th Kanazawa Bio-AFM Workshop 2016
    • Place of Presentation
      KKR ホテル金沢(石川県金沢市)
    • Year and Date
      2016-10-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生体超分子モーターの高効率なエネルギー変換のメカニズム2016

    • Author(s)
      難波啓一
    • Organizer
      東大教養部学部特別講義付録公開セミナー
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2016-09-21
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] クライオ電子顕微鏡で生体超分子の動作メカニズムを探る. Studying the mechanisms of macromolecular assemblies by electron cryomicroscopy.2016

    • Author(s)
      難波啓一
    • Organizer
      Advanced Seminar Series on Microbiology and Immunology
    • Place of Presentation
      大阪大学(大阪府吹田市)
    • Year and Date
      2016-07-15
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 生体超分子モーターの高効率なエネルギー変換メカニズム2016

    • Author(s)
      難波啓一
    • Organizer
      応用物理学セミナー
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2016-07-07
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Structures and functions of macromolecular motor assemblies.2016

    • Author(s)
      Namba, K.
    • Organizer
      2016 Kuo Symposium on 3D Cryo-EM Molecular Imaging
    • Place of Presentation
      Beiijing Gubei Water Town Hotel, Beijing, China
    • Year and Date
      2016-06-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Methodologies that Drive the Field Forward.2016

    • Author(s)
      Namba, K.
    • Organizer
      GRC on 3DEM
    • Place of Presentation
      The Chinese University of Hong Kong, Hon Kong, China
    • Year and Date
      2016-06-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] High resolutions structural analysis of the 100S ribosome.2016

    • Author(s)
      Kato, T., Miyata, T., Ueta, M., Wada, A., Wada, C. & Namba, K.
    • Organizer
      GRC on 3DEM
    • Place of Presentation
      The Chinese University of Hong Kong, Hon Kong, China
    • Year and Date
      2016-06-19
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生命科学におけるクライオ電子顕微鏡の役割とポテンシャル2016

    • Author(s)
      難波啓一
    • Organizer
      大阪大学蛋白質研究所「マルチスケール構造生物学(日本電子)」寄附研究部門開設記念セミナー
    • Place of Presentation
      大阪大学(大阪府吹田市)
    • Year and Date
      2016-06-17
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 高速AFMによるべん毛モーター固定子複合体MotPSの動的構造解析2016

    • Author(s)
      寺原直矢, 古寺哲幸, 内橋貴之, 安藤敏夫, 難波啓一, 南野徹
    • Organizer
      第四回宮田新学術領域班会議
    • Place of Presentation
      長崎大学(長崎県長崎市)
    • Year and Date
      2016-06-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] 蛋白質輸送体の中に存在する、回転分子モーターによく似た複合体. Protein translocator resembling the FI-rotary motor.2016

    • Author(s)
      寺島浩行, 川元晃大, 巽千夏, 難波啓一, 南野徹, 今田勝巳
    • Organizer
      第16回日本蛋白質科学会年会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2016-06-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] 筋収縮制御メカニズムの解明を目指した骨格筋の細いフィラメントの構造解析. GryoEM structural analysis of muscle thin filament composed of actin filament, tropomyosin and troponin.2016

    • Author(s)
      山田有里佳, 難波啓一, 藤井高志
    • Organizer
      第16回日本蛋白質科学会年会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2016-06-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] べん毛基部体の回転対称性の謎に迫る2016

    • Author(s)
      川本晃大, 宮田知子, 加藤貴之, 南野徹, 難波啓一
    • Organizer
      第四回宮田新学術領域班会議
    • Place of Presentation
      長崎大学(長崎県長崎市)
    • Year and Date
      2016-06-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] ユビキチン化に伴う基礎蛋白質の構造不安定化とアミロイド様繊維形成. ubiquitylation-driven folding destabilization of substrate proteins and amyloid-like fibril formation of polyubiquitin chains.2016

    • Author(s)
      森本大智, Erik Walinda, 深田はるみ, 菅瀬謙治, 星野大, 藤井高志, 難波啓一, 小松雅昭, 田中啓二, 白川昌宏
    • Organizer
      第16回日本蛋白質科学会年会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2016-06-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] クライオ電顕単粒子解析法による赤痢菌タイプⅢ型輸送装置ニードル繊維の2.7Å分解能での構造解析.2.7Å structure of the type 3 secretion needle by CryoEM.2016

    • Author(s)
      藤井高志, 山田有里佳, 難波啓一
    • Organizer
      第16回日本蛋白質科学会年会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2016-06-07
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] サルモネラFliFG融合変異型べん毛モーターの動態機能計測2016

    • Author(s)
      酒井智史, 森広一郎, 宮田知子, 井上由美, 寺原直矢, 森本雄祐, 加藤貴之, 難波啓一, 南野徹
    • Organizer
      第13回21世紀大腸菌研究会
    • Place of Presentation
      グリーンピア南阿蘇(熊本県阿蘇郡)
    • Year and Date
      2016-06-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] バクテリアべん毛フックの長さ制御におけるFlhAとFliH/FliI複合体の役割.2016

    • Author(s)
      井上由美, 木下実紀, 難波啓一, 南野徹.
    • Organizer
      第13回21世紀大腸菌研究会
    • Place of Presentation
      グリーンピア南阿蘇(熊本県阿蘇郡)
    • Year and Date
      2016-06-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] バクテリアべん毛フック長制御蛋白質FliKの部位特異的in vivo光架橋実験2016

    • Author(s)
      木下実紀, 難波啓一, 南野徹
    • Organizer
      第13回21世紀大腸菌研究会
    • Place of Presentation
      グリーンピア南阿蘇(熊本県阿蘇郡)
    • Year and Date
      2016-06-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] 生体超分子モーターの高効率なエネルギー変換メカニズム2016

    • Author(s)
      難波啓一
    • Organizer
      第53回レプトスピラ・シンポジウム
    • Place of Presentation
      大阪大学(大阪府吹田市)
    • Year and Date
      2016-03-22
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Structural and functional analysis of FliP as a component of the flagellar protein export gate2016

    • Author(s)
      福村拓真
    • Organizer
      2015年度べん毛研究交流会
    • Place of Presentation
      滝の湯(山形県天童市)
    • Year and Date
      2016-03-07
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] Effects of flagellin subunit linkage during protein export on flagellar filament assembly2016

    • Author(s)
      Abraham, A.
    • Organizer
      2015年度べん毛研究交流会
    • Place of Presentation
      滝の湯(山形県天童市)
    • Year and Date
      2016-03-07
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] Functional analysis of a FliFG deletion-fusion mutant flagellar motor2016

    • Author(s)
      酒井智史
    • Organizer
      2015年度べん毛研究交流会
    • Place of Presentation
      滝の湯(山形県天童市)
    • Year and Date
      2016-03-06
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] Molecular basis for dynamic structural properties of the C ring in the bacterial flagellar motor2016

    • Author(s)
      Kihoshita, M., Furukawa, Y., Imada, K., Namba, K., Minamino, T.
    • Organizer
      GRC on Sensory Transduction in Microorganisms
    • Place of Presentation
      Ventura Beach Marriott, Ventura, California, USA
    • Year and Date
      2016-01-17
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] FliS chaperone contributes to efficient unfolding of flagellin subunit during protein translocation2016

    • Author(s)
      Minamino, T., Furukawa, Y., Inoue, Y., Namba, K.
    • Organizer
      GRC on Sensory Transduction in Microorganisms
    • Place of Presentation
      Ventura Beach Marriott, Ventura, California, USA
    • Year and Date
      2016-01-17
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] クライオ電子顕微鏡による生命科学研究の最先端2016

    • Author(s)
      難波啓一
    • Organizer
      第29回日本放射光学会年会・放射光科学合同シンポジウム企画講演「細胞から生体高分子までの時空間改装イメージングと放射光」
    • Place of Presentation
      柏の葉カンファレンスセンター(千葉県柏市)
    • Year and Date
      2016-01-10
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 高速AFMによるべん毛モーター固定子複合体MotPSの動的構造解析2016

    • Author(s)
      寺原直矢, 古寺哲幸, 内橋貴之, 安藤敏夫, 難波啓一, 南野徹
    • Organizer
      2016年生体運動合同班会議
    • Place of Presentation
      キャンパスプラザ京都(京都府京都市)
    • Year and Date
      2016-01-10
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] サルモネラ属菌FliFG融合変異型べん毛モーターの動態機能計測2016

    • Author(s)
      酒井智史, 森広一郎, 宮田知子, 西山雅祥, 井上由美, 寺原直矢, 森本雄祐, 加藤貴之, 難波啓一, 南野徹
    • Organizer
      2016年生体運動合同班会議
    • Place of Presentation
      キャンパスプラザ京都(京都府京都市)
    • Year and Date
      2016-01-10
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] クライオ電子顕微鏡による構造生命科学や創薬の革新的発展と未来展望2015

    • Author(s)
      難波啓一
    • Organizer
      日本製薬工業会
    • Place of Presentation
      日本橋ライフサイエンスビルディング(東京都中央区)
    • Year and Date
      2015-12-21
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Structures and functions of macromolecular motor assemblies2015

    • Author(s)
      Namba, K.
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaji Convention Center, Honolulu, Hawaii, USA
    • Year and Date
      2015-12-16
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 生体超分子モーターの高効率なエネルギー変換のメカニズム2015

    • Author(s)
      難波啓一
    • Organizer
      新薬理学セミナー2015「分子の構造・機能をX線と電子線で見る」
    • Place of Presentation
      山口県国際総合センター海峡メッセ下関(山口県下関市)
    • Year and Date
      2015-11-21
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] クライオ電子顕微鏡による構造生命科学の革新的発展と未来展望2015

    • Author(s)
      難波啓一
    • Organizer
      日本学術振興会回折構造生物第169委員会第48回研究会
    • Place of Presentation
      関西学院大学大阪梅田キャンパス(大阪府北区)
    • Year and Date
      2015-11-18
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] クライオ電子顕微鏡と光学ナノ計測による分子モーターの構造・動態解析2015

    • Author(s)
      難波啓一
    • Organizer
      平成27年度第1回生物構造学研究会
    • Place of Presentation
      エッサム神田ホール(東京都千代田区)
    • Year and Date
      2015-11-17
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 生命を支える超分子ナノマシン2015

    • Author(s)
      難波啓一
    • Organizer
      第31回湯川記念講演会
    • Place of Presentation
      大阪大学(大阪府大阪市)
    • Year and Date
      2015-10-11
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] CryoEM structural analysis of the needle complex from Shigella flexneri.2015

    • Author(s)
      Kajimura, N., Makino, F., Kato, T., Blocker, A., Namba, K.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-15
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] Dynamics of bacterial flagellar filament self-assembly2015

    • Author(s)
      Abraham, A., Fukumura, T., Minamino, T., Namba, K.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-15
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] Evaluation of structural stability of membrane protein complex MotA/B by FSEC2015

    • Author(s)
      Takazaki, H., Namba, K., Fujii, T.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-15
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] The structure of the flagellar filament of magnetotactic bacterium MO-1 by electron cryomicroscopy2015

    • Author(s)
      Ruan, J., Kato, T., Namba, K.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-15
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] Nolecular basis for a structural switch of FlgN that regulates its chaperone activity.2015

    • Author(s)
      Kinoshita, M., Nakanishi, Y., Furukawa, Y., Imada, K., Namba, K., Minamino, T.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-15
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] The power of electron direct detector for electron cryotomography.2015

    • Author(s)
      川本晃大, 森本雄祐, 加藤貴之, 難波啓一
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-14
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] Brownian ratchet mechanisms of macromolecular motors.2015

    • Author(s)
      Namba, K.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-14
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] CryoEM 3D image reconstruction of the flagellar LP ring complex.2015

    • Author(s)
      Tatli, M., Miyata, T., Kato, T., Namba, K.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-14
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] GFP protein labeling for single particle image analysis.2015

    • Author(s)
      Kato, T., Terahara, N., Miyata, T., Namba, K.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-14
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] Structure and function of P1 adhesin of Mycoplasma pneumoniae2015

    • Author(s)
      Matsumoto, Y., Kawakita, Y., Kenri, T., Mori, S., Hamaguchi, T., Kinosita, M., Kawamoto, A., Kato, T., Namba, K., Miyata, M.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-14
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] Functional reconstitution of stator protein MotA from Aquifex aeolicus into Nanodisc2015

    • Author(s)
      Gohara, M., Takekawa, N., Terahara, N., Kato, T., Namba, K., Onoue, Y., Homma, M.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-14
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] Structural characterization of FliP, a component of flagellar type III protein export apparatus2015

    • Author(s)
      Fukumura, T., Makino, F., Kato, T., Imada, K., Namba, K., Minamino, T.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-14
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] Thermal ratchet mechanism of the bacterial flagellar motor emerged by high-resolution nanophotometry.2015

    • Author(s)
      中村修一, 森本雄祐, 上池伸徳, 曽和義幸, 南野徹, 難波啓一
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-13
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] Novel physicochemical properties of ubiquitylation2015

    • Author(s)
      森本大智, Erik Walinda, 菅瀬謙治, 深田はるみ, 曽友深, 蔭山俊, 星野大, 藤井高志, 土屋光, 佐伯泰, 有田恭平, 有吉眞理子, 栃尾豪人, 岩井一宏, 難波啓一, 小松雅明, 田中啓二, 白川昌宏
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-13
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] High-resolution cryoEM structural analysis of MotPS stator of the bacterial flagellar motor2015

    • Author(s)
      Terahara, N., Kato, T., Minamino, T., Namba, K.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-13
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] CryoEM structure of muscle thin filament with the tropomyosin and troponin complex2015

    • Author(s)
      Yamada, Y., Namba, K., Fujii, T.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-13
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] High resolution structural analysis of the flagellar hook of Salmonella.2015

    • Author(s)
      Horvath, P., Miyata, T., Takazaki, H., Kato, T., Namba, K.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-13
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] Structure of the flagellar basal boday switch complex with chemotaxis signal protein CheY-P2015

    • Author(s)
      Miyata, T., Kato, T., Morimoto, Y. V., Matsunami, H., Namba, K.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-13
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] An energy transduction mechanism of the bacterial flagellar type III secretion aparatus.2015

    • Author(s)
      Terashima, H., Kawamoto, A., Tatsumi, C., Minamino, T., Namba, K., Imada, K.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-13
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] Rail-like structure in gliding machinery of the fliding bacterium Flavobacterium johnsoniae.2015

    • Author(s)
      Shibata, S., Kawamoto, A., Kato, T., Namba, K., Nakayama, K.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-13
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] 生体超分子モーターの効率的なエネルギー変換のメカニズム2015

    • Author(s)
      難波啓一
    • Organizer
      第90回サイテックサロン
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2015-07-04
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Structures and functions of macromolecular motor assemblies.2015

    • Author(s)
      Namba, K.
    • Organizer
      KMB2015
    • Place of Presentation
      HICO, Gyeongju, Korea
    • Year and Date
      2015-06-25
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] クライオ電子顕微鏡法によるべん毛モーター固定子MotPS複合体の高分解能構造解析2015

    • Author(s)
      寺原直矢, 加藤貴之, 難波啓一
    • Organizer
      新学術領域「運動超分子マシナリーが織りなす調和と多様性」第3会領域全体会議
    • Place of Presentation
      金沢商工会議所会館(石川県金沢市)
    • Year and Date
      2015-06-10
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] FliG-FliM相互作用におけるFliG-CWバイアス変異効果2015

    • Author(s)
      木下実紀, 古川進朗, 今田勝巳, 難波啓一, 南野徹
    • Organizer
      新学術領域「運動超分子マシナリーが織りなす調和と多様性」第3会領域全体会議
    • Place of Presentation
      金沢商工会議所会館(石川県金沢市)
    • Year and Date
      2015-06-10
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] 電子直接検出カメラを用いたタンパク質の構造解析2015

    • Author(s)
      川本晃大, 加藤貴之, 難波啓一
    • Organizer
      新学術領域「運動超分子マシナリーが織りなす調和と多様性」第3会領域全体会議
    • Place of Presentation
      金沢商工会議所会館(石川県金沢市)
    • Year and Date
      2015-06-10
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細菌べん毛モーター・スイッチ複合体の構造解析2015

    • Author(s)
      宮田知子, 加藤貴之, 森本雄祐, 難波啓一
    • Organizer
      第12回21世紀大腸菌研究会
    • Place of Presentation
      琵琶湖グランドホテル・京近江(滋賀県大津市)
    • Year and Date
      2015-06-05
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] 低温電子顕微鏡を用いた海洋性ビブリオ菌べん毛モーターの構造解析2015

    • Author(s)
      川本晃大, 宮田知子, 堀田秀鷹, 稲葉敏, 森本雄祐, 西岡典子, 小嶋誠司, 加藤貴之, 本間道夫, 難波啓一
    • Organizer
      第12回21世紀大腸菌研究会
    • Place of Presentation
      琵琶湖グランドホテル・京近江(滋賀県大津市)
    • Year and Date
      2015-06-04
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] クライオ電子顕微鏡法によるべん毛モーター固定子MotPS複合体の高分解能構造解析2015

    • Author(s)
      寺原直矢, 加藤貴之, 南野徹, 難波啓一
    • Organizer
      第12回21世紀大腸菌研究会
    • Place of Presentation
      琵琶湖グランドホテル・京近江(滋賀県大津市)
    • Year and Date
      2015-06-04
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] バクテリアFlgNシャペロンの機能構造スイッチの分子基盤2015

    • Author(s)
      木下実紀, 中西雄紀, 難波啓一, 今田勝巳, 南野徹
    • Organizer
      第12回21世紀大腸菌研究会
    • Place of Presentation
      琵琶湖グランドホテル・京近江(滋賀県大津市)
    • Year and Date
      2015-06-04
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] High resolutional analysis by motion correction.2015

    • Author(s)
      加藤貴之, 難波啓一
    • Organizer
      日本顕微鏡学会第71回学術講演会
    • Place of Presentation
      国立京都国際会館(京都府京都市)
    • Year and Date
      2015-05-15
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 細菌べん毛モーターの回転機構におけるプロトン透過の役割2015

    • Author(s)
      難波啓一
    • Organizer
      分子研研究会「膜タンパク質内部のプロトン透過を考える」
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県岡崎市)
    • Year and Date
      2015-04-20
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] クライオ電子顕微鏡法によるNa+駆動型べん毛モーター固定MotPS複合体の高分解能構造解析2015

    • Author(s)
      寺原直矢, 加藤貴之, 南野徹, 難波啓一
    • Organizer
      分子研研究会「膜タンパク質内部のプロトン透過を考える」
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県岡崎市)
    • Year and Date
      2015-04-20
    • Description
      【発表確定】
    • Related Report
      2015 Annual Research Report
  • [Presentation] マイコプラズマ・モービレの滑走装置の内部構造とその変化2015

    • Author(s)
      西川実志, 中根大介, 川元晃大, 加藤貴之, 難波啓一, 宮田真人
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      長良川国際会議場(岐阜県)
    • Year and Date
      2015-03-26 – 2015-03-27
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] 細菌べん毛のIII型分泌装置の再構築2015

    • Author(s)
      寺島浩行, 川本晃大, 巽千夏, 南野徹, 難波啓一, 今田勝巳
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      長良川国際会議場(岐阜県)
    • Year and Date
      2015-03-26
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] 固定子複合体MotPSのクライオ電子顕微鏡による構造解析2015

    • Author(s)
      寺原直矢
    • Organizer
      2014年度べん毛研究交流会
    • Place of Presentation
      合歓の郷リゾートホテル(三重県)
    • Year and Date
      2015-03-02
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] サルモネラ菌におけるFljBとFliCのべん毛繊維の構造比較2015

    • Author(s)
      當間頌子
    • Organizer
      2014年度べん毛研究交流会
    • Place of Presentation
      合歓の郷リゾートホテル(三重県)
    • Year and Date
      2015-03-02
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] べん毛蛋白質輸送システムのロバストネスを支える分子機構2015

    • Author(s)
      南野徹
    • Organizer
      2014年度べん毛研究交流会
    • Place of Presentation
      合歓の郷リゾートホテル(三重県)
    • Year and Date
      2015-03-02
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] FlgNシャベロンの機能制御スイッチの同定2015

    • Author(s)
      木下実紀
    • Organizer
      2014年度べん毛研究交流会
    • Place of Presentation
      合歓の郷リゾートホテル(三重県)
    • Year and Date
      2015-03-02
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] 細菌べん毛モーターの回転方向制御に関わる構造2015

    • Author(s)
      宮田知子
    • Organizer
      2014年度べん毛研究交流会
    • Place of Presentation
      合歓の郷リゾートホテル(三重県)
    • Year and Date
      2015-03-02
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] 大腸菌べん毛モーター回転に対する高圧力の影響2015

    • Author(s)
      高木智彦, 青木里菜, 沢田孝, 梅村徹, 曽和義幸, 西山雅祥, 川岸郁朗
    • Organizer
      2014年度べん毛研究交流会
    • Place of Presentation
      合歓の郷リゾートホテル(三重県)
    • Year and Date
      2015-03-01
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] Molecular basis for dynamic structural properties of the C ring in the bacterial flagellar motor2015

    • Author(s)
      Minamino, T.
    • Organizer
      HFSP Japan Meeting
    • Place of Presentation
      ルスツリゾート(北海道)
    • Year and Date
      2015-02-24
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification of a structural switch of FlgN to control its chaperone activity2015

    • Author(s)
      Kinoshita, M.
    • Organizer
      HFSP Japan Meeting
    • Place of Presentation
      ルスツリゾート(北海道)
    • Year and Date
      2015-02-24
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] GFP labeling for single particle analysis with cryoEM2015

    • Author(s)
      Kato, T.
    • Organizer
      HFSP Japan Meeting
    • Place of Presentation
      ルスツリゾート(北海道)
    • Year and Date
      2015-02-24
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] Purification and structural analysis of FliF2015

    • Author(s)
      Kawamoto, A.
    • Organizer
      HFSP Japan Meeting
    • Place of Presentation
      ルスツリゾート(北海道)
    • Year and Date
      2015-02-23
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] Structure analysis of the flagellar hook-basal body with the C ring by electron cryo-microscopy2015

    • Author(s)
      Miyata, T.
    • Organizer
      HFSP Japan Meeting
    • Place of Presentation
      ルスツリゾート(北海道)
    • Year and Date
      2015-02-23
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] Structural characterization of FliP, a component of the type III flagellar protein export apparatus2015

    • Author(s)
      Fukumura, T., Makino, F., Kato, T., Imada, K., Namba, K. & Minamino, T.
    • Organizer
      BLAST XIII
    • Place of Presentation
      Hilton Tucson East, USA
    • Year and Date
      2015-01-18 – 2015-01-23
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] バクテリアベん毛蛋白質輸送におけるFliI ATPaseの作用機構2015

    • Author(s)
      南野徹, 森本雄祐, 木下実紀, 難波啓一
    • Organizer
      2015年生体運動合同班会議
    • Place of Presentation
      学習院大学(東京都)
    • Year and Date
      2015-01-09
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] 細菌べん毛モーターはサーマルラチェットか?2015

    • Author(s)
      中村修一, 森本雄祐, 上池伸徳, 曽和義幸, 南野徹, 難波啓一
    • Organizer
      2015年生体運動合同班会議
    • Place of Presentation
      学習院大学(東京都)
    • Year and Date
      2015-01-08
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] CryoEM image analysis of macromolecular motor assemblies2014

    • Author(s)
      Namba, K.
    • Organizer
      Symposium-cumWorkshop “Frontiers in structural biology : New advances in X-ray diffraction and cryo-electron microscopy”
    • Place of Presentation
      Indian National Science Academy, New Delhi, India
    • Year and Date
      2014-12-16
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] CryoEM image analysis of macromolecular motor assemblies2014

    • Author(s)
      Namba, K.
    • Organizer
      FON' 14
    • Place of Presentation
      Miraikan Hall(東京都)
    • Year and Date
      2014-11-27
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 生体超分子モーターの構造と動作メカニズム2014

    • Author(s)
      難波啓一
    • Organizer
      第67回東京大学物理学教室コロキウム
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2014-11-07
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 大腸菌ヒストン様蛋白質H-NSによるべん毛モーター回転制御能の解析2014

    • Author(s)
      沢田孝, 梅村徹, 西山宗一郎, 山本兼由, 曽和義幸, 川岸郁朗
    • Organizer
      第97回日本細菌学会関東支部総会
    • Place of Presentation
      東京ドームホテル(東京都)
    • Year and Date
      2014-10-31
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] 生命を支える超分子ナノマシン2014

    • Author(s)
      難波啓一
    • Organizer
      第52回日本生物物理学会市民講演会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-28
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 低温電子顕微鏡による単粒子像解析法におけるGFPラベル. GFP labeling for single Particle analysis with cryoEM2014

    • Author(s)
      Kato, T., Terahara, N., Miyata, T., Namba, K.
    • Organizer
      第52回日本生物物理学会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-26
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] 赤痢菌ニードル複合体の極低温電子顕微鏡による構造解析. Structural analysis of needle complex from shigella flexneri by cryo electron microscopy2014

    • Author(s)
      Kajimura, N., Makino, F., Cheung, M.P., Kato, T., Blocker, A.J., Namba, K.
    • Organizer
      第52回日本生物物理学会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-26
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] 海洋性細菌Vibrio alginolyticusのべん毛基部体T-ringの低温電子顕微鏡を用いた構造解析. Structural analysis of T-ring in the flagellar basal body of Vibrio alginolyticus by electron cryomicroscopy2014

    • Author(s)
      Hotta, H., Kawamoto, A., Inaba, S., Morimoto, Y.V., Nishioka, N., Kojima, S., Namba, K., Homma, M.
    • Organizer
      第52回日本生物物理学会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-26
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] Structure of the bacterial flagellar motor inv olved in the directional switching mechanism細菌べん毛モーターの回転方向変換制御に関わる構造2014

    • Author(s)
      宮田知子, 加藤貴之, 森本雄祐, 中村修一, 松波秀行, 難波啓一
    • Organizer
      第52回日本生物物理学会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-26
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] 低温電子顕微鏡による骨格筋の細いフィラメントの構造解析. Structural analysis of Ca2+ regulated thin filament from skeletal muscle by electron cryomicroscopy2014

    • Author(s)
      Yamada, Y., Fujii, T., Namba, K.
    • Organizer
      第52回日本生物物理学会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-25
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] 細菌III型分泌装置のin vitro輸送再構成系の構築. Construction of an in vitro transport assay system for the bacterial type III protein secretion2014

    • Author(s)
      Terashima, H. Kawamoto, A., Minamino, T., Namba, K., Imada, K.
    • Organizer
      第52回日本生物物理学会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-25
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] クライオ電子顕微鏡による生体超分子の機能構造の解明2014

    • Author(s)
      難波啓一
    • Organizer
      第23回日本バイオイメージング学会学術集会
    • Place of Presentation
      大阪大学(大阪府)
    • Year and Date
      2014-09-05
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] CryoEM image analysis of macromolecular motor assemblies2014

    • Author(s)
      Namba, K
    • Organizer
      2014 International Biophysics Congress
    • Place of Presentation
      Brisbane Convention & Exhibition Centre, Brisbane, Australia
    • Year and Date
      2014-08-04
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] In situ structures of Salmonella injectisome and flagellar type III secretion systems by electron cryotomography2014

    • Author(s)
      Kawamoto, A., Morimoto, Y.V., Miyata, T., Minamino, T. Hughes, K.T., Kato, T., Namba, K.
    • Organizer
      2014 International Biophysics Congress
    • Place of Presentation
      Brisbane Convention & Exhibition Centre, Brisbane, Australia
    • Year and Date
      2014-08-04
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] さまざまな運動超分子の構造と機能2014

    • Author(s)
      難波啓一
    • Organizer
      新学術領域研究「運動超分子マシナリーが織りなす調和と多様性」第2回領域全体会議
    • Place of Presentation
      大雪クリスタルホール(北海道)
    • Year and Date
      2014-06-16
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] CryoEM image analysis of macromolecular motor assemblies2014

    • Author(s)
      Namba, K
    • Organizer
      The 5th Cold Spring Harbor Asia Symposium : Structural Biology from Atoms to Cells
    • Place of Presentation
      Suzhou Dushu Lake Conference Center, Suzhou, China
    • Year and Date
      2014-06-12
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 枯草菌べん毛モーターの性能は異なる2種類の固定子によって調節される2014

    • Author(s)
      寺原直矢, 野口有希奈, 中村修一, 上池伸徳, 南野徹, 伊藤政博, 難波啓一
    • Organizer
      第11回21世紀大腸菌研究会
    • Place of Presentation
      ホテル大観(岩手県)
    • Year and Date
      2014-06-06
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] バクテリアベん毛モーターの回転に共役したプロトンの流れは固定子と回転子の相互作用により制御される2014

    • Author(s)
      森本雄祐, 平岡孝一, 上池伸徳, 難波啓一, 南野徹
    • Organizer
      第11回21世紀大腸菌研究会
    • Place of Presentation
      ホテル大観(岩手県)
    • Year and Date
      2014-06-06
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] バクテリアベん毛蛋白質輸送ゲート構成因子Flipペリプラズムドメインのx線結晶構造解析2014

    • Author(s)
      福村拓真, 今田勝巳, 難波啓一, 南野徹
    • Organizer
      第11回21世紀大腸菌研究会
    • Place of Presentation
      ホテル大観(岩手県)
    • Year and Date
      2014-06-05 – 2014-06-06
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
  • [Presentation] 生命を支える超分子ナノマシン2014

    • Author(s)
      難波啓一
    • Organizer
      日本顕微鏡学会第70回市民公開講座
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2014-05-10
    • Description
      【発表確定】
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 電子線トモグラフィーによる生体試料の立体構造解析2014

    • Author(s)
      加藤貴之, Ruan Juanfang, 川本晃大, 宮田知子, 難波啓一
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス(神奈川県)
    • Year and Date
      2014-03-30
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 生体ナノマシンの動作原理の解明2014

    • Author(s)
      難波啓一
    • Organizer
      第119回日本解剖学会総会・全国学術集会解剖学会・顕微鏡学会合同シンポジウム「最近の顕微鏡法が拓く機能分子解剖学の新展開」
    • Place of Presentation
      自治医科大学(栃木県)
    • Year and Date
      2014-03-29
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] MycoplasEa mobileから単離した滑走装置の構造解析2014

    • Author(s)
      西川実志, 中根大介, 川本晃大, 加藤貴之, 難波啓一, 宮田真人
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      タワーホール船堀(東京都)
    • Year and Date
      2014-03-27
    • Related Report
      2013 Annual Research Report
  • [Presentation] サルモネラ菌ニードル複合体およびべん毛III型分泌装置の細胞内機能構造2014

    • Author(s)
      川本晃大, 森本雄祐, 宮田知子, 南野徹, Kelly T. Hughes, 加藤貴之, 難波啓一
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      タワーホール船堀(東京都)
    • Year and Date
      2014-03-26
    • Related Report
      2013 Annual Research Report
  • [Presentation] 赤痢菌ニードル複合体のクライオ電子顕微鏡による構造解析. Structure of the needle complex of Shigella flexlnelli by electron cryomicroscopy.2014

    • Author(s)
      梶村直子
    • Organizer
      2013年度・第19回べん毛研究交流会
    • Place of Presentation
      県立広島大学サテライトキャンパス(広島県)
    • Year and Date
      2014-03-03
    • Related Report
      2013 Annual Research Report
  • [Presentation] クライオ電顕トモグラフィーによるサルモネラ菌III型分泌装置の構造. Architecture of the Salmonella type III secretion system by electron cryotomography.2014

    • Author(s)
      川本晃大
    • Organizer
      2013年度・第19回べん毛研究交流会
    • Place of Presentation
      県立広島大学サテライトキャンパス(広島県)
    • Year and Date
      2014-03-03
    • Related Report
      2013 Annual Research Report
  • [Presentation] 枯草菌べん毛モーターの2種類の固定子による外環境適応機構. Environmental adaptation of Bacillus flagellar motor by MotAB and MotPS stators.2014

    • Author(s)
      寺原直矢
    • Organizer
      2013年度・第19回べん毛研究交流会
    • Place of Presentation
      県立広島大学サテライトキャンパス(広島県)
    • Year and Date
      2014-03-03
    • Related Report
      2013 Annual Research Report
  • [Presentation] サラモネラ菌べん毛の回転子蛋白質FliFG融合型モーターの機能解析. Functional analysis of the Salmonella flagellar FliFG fusion motor.2014

    • Author(s)
      森広一郎
    • Organizer
      2013年度・第19回べん毛研究交流会
    • Place of Presentation
      県立広島大学サテライトキャンパス(広島県)
    • Year and Date
      2014-03-02
    • Related Report
      2013 Annual Research Report
  • [Presentation] 高速回転ナノモーターを駆使して泳ぐ細菌たち2014

    • Author(s)
      難波啓一
    • Organizer
      日本微生物学連盟フォーラム『驚きの微生物たち』-中学生から社会人の方々のための-
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2014-02-01
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Common and distinct structural features of Salmonella injectisome and flagellar basal body.2014

    • Author(s)
      Kawamoto, A., Morimoto, Y. V., Miyata, T., Minamino, T., Hughes, K. T., Kato, T., Namba, K.
    • Organizer
      GRC on Sensory Transduction in Microorganisms
    • Place of Presentation
      Ventura Beach Marriott (USA)
    • Year and Date
      2014-01-15
    • Related Report
      2013 Annual Research Report
  • [Presentation] CheY-P binding to the bacterial flagellar motor affects not only the direction but also the speed of rotation.2014

    • Author(s)
      Hiraoka, K., Morimoto, Y. V., Fujii, T., Miyata, T., Makino. F., Minamino, T., Namba, K.
    • Organizer
      GRC on Sensory Transduction in Microorganisms
    • Place of Presentation
      Ventura Beach Marriott (USA)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Evolution of a robust protein export system for bacterial flagellar assembly.2014

    • Author(s)
      Minamino, T., Morimoto, Y. V., Namba, K.
    • Organizer
      GRC OD Sensory Transduction in Microorganisms
    • Place of Presentation
      Ventura Beach Marriott (USA)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Functional analysis of the bacterial flagellar motor in fliFG fusion mutants.2014

    • Author(s)
      Mori, K., Hiraoka, K., Morimoto, Y. V., Miyata, T., Kami-ike, N., Minamino, T., Namba, K.
    • Organizer
      GRC on Sensory Transduction in Microorganisms
    • Place of Presentation
      Ventura Beach Marriott (USA)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Evidence of the stator-rotor interaction driving proton flow coupled with torque generation in the bacterial flagellar motor.2014

    • Author(s)
      Morimoto, Y. V., Minamino, T., Namba, K.
    • Organizer
      GRC on Sensory Transduction in Microorganisms
    • Place of Presentation
      Ventura Beach Marriott (USA)
    • Related Report
      2013 Annual Research Report
  • [Presentation] In-frame deletions in the Periplasmic region of MotB cause unusual torque-speed relationship in the Salmonella flagellar motor.2014

    • Author(s)
      Nakamura, S., Morimoto, Y. V., Castillo, D. J., Che, Y. -S., Kamiike, N., Kudo, S., Minamino, T., Namba, K.
    • Organizer
      GRC on Sensory Transduction in Microorganisms
    • Place of Presentation
      Ventura Beach Marriott (USA)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Identification of a switch protein FliG in the flagellar basal body. 細菌べん毛基部体におけるスイッチ蛋白質FliGの位置と向きの同定2014

    • Author(s)
      宮田知子
    • Organizer
      2013年度・第19回べん毛研究交流会
    • Place of Presentation
      県立広島大学サテライトキャンパス(広島県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] べん毛特異的分子シャペロンと輸送ゲート構成蛋白質FlhAとの相互作用. Interaction of bacterial flagellar chaperones with an export gate protein FlhA.2014

    • Author(s)
      木下実紀
    • Organizer
      2013年度・第19回べん毛研究交流会
    • Place of Presentation
      県立広島大学サテライトキャンパス(広島県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細菌べん毛固定子回転子間相互作用によるプロトンチャネルの活性化. Evidence of stator-rotor interaction activating proton channel for torque generation by the flagellar motor.2014

    • Author(s)
      森本雄祐
    • Organizer
      2013年度・第19回べん毛研究交流会
    • Place of Presentation
      県立広島大学サテライトキャンパス(広島県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ワタリガニ骨格筋の細いフィラメントの精製および低温電子顕微鏡法による構造解析. Isolation of native thin filament from skeletal muscle for structural analysis by cryoEM.2014

    • Author(s)
      山田有里佳
    • Organizer
      2013年度・第19回べん毛研究交流会
    • Place of Presentation
      県立広島大学サテライトキャンパス(広島県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] fliFG融合変異株のべん毛モーターの機能解析2013

    • Author(s)
      森広一郎, 平岡孝一, 森本雄祐, 宮田知子, 上池伸徳, 南野徹, 難波啓一
    • Organizer
      日本生体エネルギー研究会第39回討論会
    • Place of Presentation
      静岡県コンベンションアーツセンター(静岡県)
    • Year and Date
      2013-12-19
    • Related Report
      2013 Annual Research Report
  • [Presentation] グラム陽性-陰性細菌間におけるべん毛モーター固定子の異種発現とトルク特性の解析2013

    • Author(s)
      寺原直矢, 上池伸徳, 難波啓一, 南野徹
    • Organizer
      日本生体エネルギー研究会第39回討論会
    • Place of Presentation
      静岡県コンベンションアーツセンター(静岡県)
    • Year and Date
      2013-12-19
    • Related Report
      2013 Annual Research Report
  • [Presentation] 生命を支える超分子ナノマシンの機能構造の解明に向けて2013

    • Author(s)
      難波啓一
    • Organizer
      日本顕微鏡学会・関西支部「若手の会」設立記念講演会
    • Place of Presentation
      KKRホテルびわこ(滋賀県)
    • Year and Date
      2013-12-07
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 細菌べん毛ディスタルロッドの構造と力学的特性2013

    • Author(s)
      西條由見子, 今田勝巳, 松波秀行, 藤井高志, 難波啓一
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2013-12-05
    • Related Report
      2013 Annual Research Report
  • [Presentation] クライオ電子顕微鏡による生体超分子ナノマシンの機能構造の解明に向けて. High-resolution high-throughput cryoEM image analysis of macromolecular assemblies.2013

    • Author(s)
      難波啓一
    • Organizer
      第36回日本分子生物学会年会フォーラム
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2013-12-03
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ワタリガニ骨格筋における細いフィラメントの精製および低温電子顕微鏡法による構造解析. Isolation of native thin filament from skeletal muscle for structural analysis by cryoEM.2013

    • Author(s)
      Yamada, Y., Fujii, T., Namba, K.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2013-10-30
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細菌べん毛輸送装置構成蛋白質FlhAの変異に対するロバストネス. Mutational robustness of FlhA, a subunit of the bacterial flagellar export apparatus.2013

    • Author(s)
      Minamino, T., Kinoshita, M., Hara, N., Koya, S., Nishioka, N., Kojima, S., Ihara, K., Homma, M., Namba, K.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2013-10-30
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細菌べん毛モーターへのCheY-Pの結合は回転方向だけでなく速度にも影響する. CheY-P binding to the bacterial flagellar motor affects not only the direction but also the speed of rotation.2013

    • Author(s)
      Hiraoka, K. D., Nakamura, S., Kami-ike, N., Morimoto, Y. V.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2013-10-30
    • Related Report
      2013 Annual Research Report
  • [Presentation] べん毛輸送装置構成蛋白質FliPペリプラズミックループの結晶化と遺伝学的解析. Crystallization and genetic analyses of a Periplasmic loop of Flip, a component of the flageliar protein exort apparatus.2013

    • Author(s)
      Fukumura, T., Saijo-Hamano, Y., Furukawa, Y., Kwaguchi, T., Imada, K., Namba, K., Minamino, T.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2013-10-29
    • Related Report
      2013 Annual Research Report
  • [Presentation] Structural study of the sheath in the magneto tactic bacterium MO-1 by electron cryomicroscopy.2013

    • Author(s)
      Ruan, J., Kato, T., Santini, C. -L., Wu, L. -F., Namba, K.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2013-10-29
    • Related Report
      2013 Annual Research Report
  • [Presentation] Torque-speed relationship of the flagellar motor consisting of two distinct stators.2013

    • Author(s)
      Terahara, N., Noguchi, Y., Nakamura, S., Kami-ike, N., Minamino, T., Ito, M., Namba, K.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2013-10-29
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細菌べん毛ディスタルロッドの構造解析. High-resolution structure of the bacterial flagellar distal rod.2013

    • Author(s)
      西條由見子, 今田勝巳, 松波秀行, 藤井高志, 難波啓一
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2013-10-28
    • Related Report
      2013 Annual Research Report
  • [Presentation] 自然免疫非感受性のサルモネラ菌FljBが形成するべん毛繊維の立体構造とFliCべん毛繊維との違い. CryoEM structure of the flagellar filament of Salmonella FljB and implication of its difference from the FliC filament.2013

    • Author(s)
      Toma, S., Kato, T., Namba, K.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2013-10-28
    • Related Report
      2013 Annual Research Report
  • [Presentation] 赤痢菌ニードル複合体の極低温電子顕微鏡による構造解析. Structural analysis of needle complex from shigella flexineri by cryo microscopy.2013

    • Author(s)
      Kajimura, N., Cheung, M. P., Kato, T., Blocker, A. J., Namba, K.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2013-10-28
    • Related Report
      2013 Annual Research Report
  • [Presentation] 高分解能構造解析に向けた電子顕微鏡用カメラの評価. Evaluation of cameras for high resolution structural analysis by cryoEM.2013

    • Author(s)
      Kato, T., Miyata, T., Namba, K.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2013-10-28
    • Related Report
      2013 Annual Research Report
  • [Presentation] アクチンフィラメントの伸張メカニズムを解明するための分子シミュレーション. A molecular simulation study to investigate actin firament elongation mechanism.2013

    • Author(s)
      Wakai, N., Takemura, K., Fujii, T., Namba, K., Kitao, A.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2013-10-28
    • Related Report
      2013 Annual Research Report
  • [Presentation] ダイニン-微小管インターフェイスの構造解析 : 徴小管からAAA+ATPaseドメインにどのように情報が伝えられるか? structural analysis of dynein-microtubule interface : How is a signal transmitted from microtubule to AAA+ATPase domain?2013

    • Author(s)
      Uchimura, S., Fujii, T., Takazaki, H., Ayukawa, R., Minoura, I., Nishikawa, Y., Hachikubo Y., Kon, T., Kurisu, G., Sutoh, K., Namba, K., Muto, E.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2013-10-28
    • Related Report
      2013 Annual Research Report
  • [Presentation] MotBペリプラスム領域のin-flame欠損がサルモネラ菌べん毛モーターの出力特性に及ぼす影響. Effect to in-frame deletion in the Periplasinic region of MotB on the torgue-speed relationship of Salmonella flagellar motor.2013

    • Author(s)
      Nakamura, S., Morimoto, Y. V., Castillo, D. J., Che, Y. -S., Kami-ike, N. Kudo, S., Minamino, T., Namba, K.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2013-10-28
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細菌べん毛モーター蛋白質FliG-FliM相互作用の解析. Interaction between FliG and FliM ID the bacterial flagellar motor.2013

    • Author(s)
      Kinoshita, M., Furukawa, Y., Imada, K., Namba, K., Minamino, T.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2013-10-28
    • Related Report
      2013 Annual Research Report
  • [Presentation] クライオ電子顕微鏡によるべん毛蛋白質輸送装置の構造と分子機構. Molecular mechanism of the type III protein export by electron cryotomography of the flagellar basal body.2013

    • Author(s)
      Kawamo, A., Miyata, T., Morimoto, Y. V., Minamino, T., Kato, T., Namba, K.
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2013-10-28
    • Related Report
      2013 Annual Research Report
  • [Presentation] CryoEM image analysis of macromolecular motor assemblies.2013

    • Author(s)
      Namba, K.
    • Organizer
      ComBio 2013
    • Place of Presentation
      Perth Convention and Exhibition Centre, Perth, Australia
    • Year and Date
      2013-09-30
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] クライオ電子顕微鏡による超分子・細胞の3Dイメージング2013

    • Author(s)
      難波啓一
    • Organizer
      日本学術会議公開シンポジウム「医学・生命科学の革新的発展に資する統合バイオイメージングの展望」
    • Place of Presentation
      日本学術会議(東京都)
    • Year and Date
      2013-09-17
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] CryoEM image analysis of macromolecular motor assemblies.2013

    • Author(s)
      Namba, K.
    • Organizer
      BSCB Autumn Meeting
    • Place of Presentation
      Low Wood Bay Hotel, Windermere, UK
    • Year and Date
      2013-09-03
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] クライオ電子顕微鏡による生体超分子ナノマシンの機能構造の解明に向けて2013

    • Author(s)
      難波啓一
    • Organizer
      第7回細菌学若手コロッセウム
    • Place of Presentation
      広島エアポートホテル(広島県)
    • Year and Date
      2013-08-08
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] クライオ電子線トモグラフィー : 細胞まるごと立体化2013

    • Author(s)
      加藤貴之
    • Organizer
      新学術領域研究「運動超分子マシナリーが織りなす調和と多様性」第一回全体会議
    • Place of Presentation
      名古屋大学(愛知県)
    • Year and Date
      2013-06-29
    • Related Report
      2013 Annual Research Report
  • [Presentation] 低温電子顕微鏡による生体試料の構造解析~分子構造から細胞内構造~2013

    • Author(s)
      加藤貴之
    • Organizer
      新学術領域研究「運動超分子マシナリーが織りなす調和と多様性」第一回全体会議
    • Place of Presentation
      名古屋大学(愛知県)
    • Year and Date
      2013-06-29
    • Related Report
      2013 Annual Research Report
  • [Presentation] 異なる2種類の固定子を持つ枯草菌べん毛モーターの1分子回転計測2013

    • Author(s)
      寺原直矢
    • Organizer
      新学術領域研究「運動超分子マシナリーが織りなす調和と多様性」第一回全体会議
    • Place of Presentation
      名古屋大学(愛知県)
    • Year and Date
      2013-06-28
    • Related Report
      2013 Annual Research Report
  • [Presentation] CryoEM helical image analysis of various macromolecular assemblies.2013

    • Author(s)
      Namba, K.
    • Organizer
      GRC on Three Dimensional Electron Microscopy
    • Place of Presentation
      Colby-Sawyer College, New London, USA
    • Year and Date
      2013-06-26
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 細菌べん毛タンパク質輸送装置の構造解析2013

    • Author(s)
      川本晃大, 森本雄祐, 宮田知子, 南野徹, Kelly T. Hughes, 加藤貴之, 難波啓一
    • Organizer
      第10回21世紀大腸菌研究会
    • Place of Presentation
      ラフォーレ修善寺(静岡県)
    • Year and Date
      2013-06-20
    • Related Report
      2013 Annual Research Report
  • [Presentation] 球状-繊維状繊維シミュレーションによるアクチンフィラメントの伸張メカニズム. Elongation mechanism of actin filament investigated by globular-filamentous transition simulation.2013

    • Author(s)
      Wakai, N., Takemura, K., Fujii, T., Namba, K., Kitao, A.
    • Organizer
      第13回日本蛋白質科学会年会
    • Place of Presentation
      とりぎん文化会館(鳥取県)
    • Year and Date
      2013-06-12
    • Related Report
      2013 Annual Research Report
  • [Presentation] ナノスペースでの生命のしくみ2013

    • Author(s)
      難波啓一
    • Organizer
      医学振興銀杏会総会
    • Place of Presentation
      大阪大学銀杏会館(大阪府)
    • Year and Date
      2013-05-25
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 細菌べん毛モーターの回転方向変換制御機構の解明. Elucidation of the rotational switch mechanism of the bacterial flagellar motor.2013

    • Author(s)
      宮田知子, 加藤貴之, 藤井高志, 森本雄祐, 中村修一, 南野徹, 松波秀行, 難波啓一
    • Organizer
      日本顕微鏡学会第69回学術講演会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府)
    • Year and Date
      2013-05-21
    • Related Report
      2013 Annual Research Report
  • [Presentation] 電子線トモグラフィーによって明らかとなった細菌べん毛蛋白質輸送装置の構造. Structure of export apparatus revealed by electron cryotomography of minicells.2013

    • Author(s)
      川本晃大, 森本雄祐, 加藤貴之, Hughes T. Kelly, 南野徹, 難波啓一
    • Organizer
      日本顕微鏡学会第69回学術講演会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府)
    • Year and Date
      2013-05-21
    • Related Report
      2013 Annual Research Report
  • [Presentation] サルモネラ菌におけるFljBとFliCのべん毛繊維の構造比較. Differences between the Salmonella FljB and FliC flagellar filament structures.2013

    • Author(s)
      當間頌子, 加藤貴之, 難波啓一
    • Organizer
      日本顕微鏡学会第69回学術講演会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府)
    • Year and Date
      2013-05-21
    • Related Report
      2013 Annual Research Report
  • [Presentation] クライオ電子顕微鏡による生体超分子ナノマシンの機能構造の解明. High-resolution high-throughput cryoEM helical image analysis of macromolecular assemblies.2013

    • Author(s)
      難波啓一
    • Organizer
      第六回風戸賞受賞講演会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府)
    • Year and Date
      2013-05-20
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 生体超分子ナノマシンの構造と機能の解明に向けて2013

    • Author(s)
      難波啓一
    • Organizer
      第60回日本生化学会近畿支部例会
    • Place of Presentation
      大阪大学銀杏会館(大阪府)
    • Year and Date
      2013-05-18
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Structures and functions of macromolecular assemblies by electron cryomicroscopy - the potential for biological sciences-2013

    • Author(s)
      Namba, K.
    • Organizer
      Medicine & Life Science seminar
    • Place of Presentation
      Kumamoto University(熊本県)
    • Year and Date
      2013-04-24
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] liFG融合変異株のべん毛モーターの機能解析. 第10回21世紀大腸菌研究会2013

    • Author(s)
      森広一郎, 平岡孝一, 森本雄祐, 宮田知子, 上地伸徳, 南野徹, 難波啓一
    • Organizer
      第10回21世紀大腸菌研究会
    • Place of Presentation
      ラフォーレ修善寺(静岡県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] A molecular simulation study to investigate actin filament elongation mechanism.2013

    • Author(s)
      Wakai, N., Nishihara, Y., Takemura, K., Fujii, T., Namba, K., Kitao, A.
    • Organizer
      3rd International Conference on Molecular Simulation
    • Place of Presentation
      Kobe International Conference Center, Hyogo
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of the new CryoEM, named “CryoARM”.

    • Related Report
      2018 Annual Research Report
  • [Presentation] Structure and rotational symmetry of the rotor of the bacterial flagellar motor revealed by electron cryomicroscopy.

    • Related Report
      2018 Annual Research Report
  • [Presentation] Towards atomic resolution structural analysis of biomolecules by electron cryomicroscopy.

    • Related Report
      2018 Annual Research Report
  • [Presentation] Recent progress of electron cryomicroscopy for structural analysis of biomolecular assemblies at atomic level.

    • Related Report
      2018 Annual Research Report
  • [Presentation] Cryo-electron microscopy study toward detecting domain motion of glutamate dehydrogenase.

    • Related Report
      2018 Annual Research Report
  • [Presentation] The cap structure of the bacterial flagellar filament.

    • Related Report
      2018 Annual Research Report
  • [Presentation] Structure and rotational symmetry of the rotor of the bacterial flagellar motor revealed by electron cryomicroscopy.

    • Related Report
      2018 Annual Research Report
  • [Presentation] Autonomous stator remodeling mechanism of the bacterial flagellar motor.

    • Related Report
      2018 Annual Research Report
  • [Presentation] The dependence of the speed of the bacterial flagellar motor on the number of stator units.

    • Related Report
      2018 Annual Research Report
  • [Presentation] Gwatch : the pipeline program for quick evaluation of sample quality in CryoEM.

    • Related Report
      2018 Annual Research Report
  • [Presentation] Structure of the growing end of the bacterial flagellar filament.

    • Related Report
      2018 Annual Research Report
  • [Presentation] Rotational symmetry structure of the bacterial flagellar motor for torque transmission revealed by electron cryomicroscopy.

    • Related Report
      2018 Annual Research Report
  • [Presentation] Screening method for samples for high resolution structural analysis by cryoEM.

    • Related Report
      2018 Annual Research Report
  • [Presentation] Structural comparison between Salmonella flagellar filaments consisting of FliC and FljB and the implication for their functions.

    • Related Report
      2018 Annual Research Report
  • [Presentation] Visualizing the domain motion of Glutamate Dehydrogenase by using cryo-electron microscopy.

    • Related Report
      2018 Annual Research Report
  • [Presentation] Elucidation of the directional switching mechanism of the bacterial flagellar motor by electron cryomicroscopy.

    • Related Report
      2018 Annual Research Report
  • [Presentation] Structural and functional analyses of the bacterial flagellar type Ⅲ export gate complex.

    • Related Report
      2018 Annual Research Report
  • [Presentation] Structural analysis of Salmonella's “native” hook.

    • Related Report
      2018 Annual Research Report
  • [Presentation] Elucidation of the directional switching mechanism of the bacterial flagellar motor by electron cryomicroscopy.

    • Related Report
      2018 Annual Research Report
  • [Presentation] Comparison of Salmonella flagellar filaments composed of FljB and FliC.

    • Related Report
      2018 Annual Research Report
  • [Presentation] Ion selectivity in the periplasmic domain of the stator complex.

    • Related Report
      2018 Annual Research Report
  • [Book] Salmonella flagellum.2018

    • Author(s)
      Minamino, T., Morimoto, Y. V., Kawamoto, A., Terashima, H. & Imada, K.
    • Pages
      3-18
    • Total Pages
      120
    • Publisher
      INTECHOPEN LIMITED
    • Related Report
      2018 Annual Research Report
  • [Book] 生化学第90巻第2号「細菌べん毛特異的輸送シャペロンFlgNの構造と機能」2018

    • Author(s)
      南野徹, 木下実紀
    • Pages
      203-206
    • Total Pages
      131
    • Publisher
      日本生化学会
    • Related Report
      2018 Annual Research Report
  • [Book] 顕微鏡Vol. 53 No. 1「生命を解き明かすクライオ電子顕微鏡法の新時代 最新クライオ電子顕微鏡CRYO ARMの開発」2018

    • Author(s)
      加藤貴之, 難波啓一.
    • Pages
      13-17
    • Total Pages
      54
    • Publisher
      日本顕微鏡学会
    • Related Report
      2018 Annual Research Report
  • [Book] 日本電子newsVol. 50 No. 1「クライオ電子顕微鏡法の技術開発と生命科学への貢献」2018

    • Author(s)
      難波啓一, 加藤貴之.
    • Pages
      2-7
    • Total Pages
      50
    • Publisher
      日本電子株式会社
    • Related Report
      2018 Annual Research Report
  • [Book] JEOL NEWS, Vol. 53, No. 1 "Technical Development of Electron Cryomicroscopy and Contributions to Life Sciences."2018

    • Author(s)
      Namba, K., Kato, T.
    • Pages
      18-24
    • Total Pages
      63
    • Publisher
      日本電子株式会社
    • Related Report
      2018 Annual Research Report
  • [Book] Encyclopedia of Biophysics "Electron Microscopy of Motor Structure and Possible Mechanisms"2018

    • Author(s)
      Minamino, T., Kato, T., Makino, F., Horvath, P., Miyata, T, & Namba, K.
    • Pages
      18-18
    • Total Pages
      73
    • Publisher
      Springer-Verlag Berlin Heidelberg
    • Related Report
      2018 Annual Research Report
  • [Book] FEMS Microbiology letters Vol. 365, No. 12 "Hierarchical protein export mechanism of the bacterial flagellar type Ⅲ protein export apparatus"2018

    • Author(s)
      Minamino, T.
    • Publisher
      OXFORD ACADEMIC
    • Related Report
      2018 Annual Research Report
  • [Book] Integrative Structural Biology with Hybrid Methods, Advances in Experimental Medicine and Biology 1105, Chapter 4 "Complementary use of electron cryomicroscopy and X-ray crystallography : structural studies of actin and actomyosin filaments."2018

    • Author(s)
      Fujii, T., Namba, K.
    • Pages
      25-42
    • Total Pages
      272
    • Publisher
      Springer Nature Singapore Pte Ltd.
    • Related Report
      2018 Annual Research Report
  • [Book] In Tech “The bacterial flagellum”2018

    • Author(s)
      Minamino, T., Morimoto, Y. V., Kawamoto, A., Terashima, H. & Imada, K.
    • Publisher
      INTECHOPEN LIMITED
    • Related Report
      2017 Annual Research Report
  • [Book] Encyclopedia of Biophysics “Microscopy of Motor Structure and Possible Mechanisms.”2018

    • Author(s)
      Minamino, T., Kato, T., Makino, F., Horvath, P., Miyata, T. & Namba, K.
    • Publisher
      Springer-Verlag Berlin Heidelberg
    • Related Report
      2017 Annual Research Report
  • [Book] Fuel of the bacterial flagellar type ⅠⅠⅠ protein export apparatus. Methods in Molecular Biology (The Bacterial Flagellum - Methods and Protocols- ; Minamino, T. & Namba, K. eds.)2017

    • Author(s)
      Minamino, T., Kinoshita, M., Namba, K.
    • Pages
      3-16
    • Total Pages
      1593
    • Publisher
      Humana Press. Springer Nature
    • Related Report
      2017 Annual Research Report
  • [Book] Structural study of the bacterial flagellar basal body by electron cryomicroscopy and image analysis. Methods in Molecular Biology (The Bacterial Flagellum - Methods and Protocols- ; Minamino, T. & Namba, K. eds.)2017

    • Author(s)
      Kawamoto, A., Namba, K.
    • Pages
      119-131
    • Total Pages
      1593
    • Publisher
      Humana Press. Springer Nature
    • Related Report
      2017 Annual Research Report
  • [Book] Stoichiometry and turnover of the stator and rotor. Methods in Molecular Biology (The Bacterial Flagellum - Methods and Protocols- ; Minamino, T. & Namba, K. eds.)2017

    • Author(s)
      Morimoto, Y. V., Minamino, T.
    • Pages
      203-213
    • Total Pages
      1593
    • Publisher
      Humana Press. Springer Nature
    • Related Report
      2017 Annual Research Report
  • [Book] 現代化学2017年12月号「2017年ノーベル化学賞 クライオ電子顕微鏡法の開発」2017

    • Author(s)
      加藤貴之, 難波啓一.
    • Pages
      40-44
    • Total Pages
      80
    • Publisher
      株式会社東京化学同人
    • Related Report
      2017 Annual Research Report
  • [Book] 生物物理57巻「バクテリア1細胞内における局所pH計測」2017

    • Author(s)
      森本雄祐, 上池伸徳, 難波啓一, 南野徹.
    • Pages
      296-298
    • Total Pages
      336
    • Publisher
      生物物理
    • Related Report
      2017 Annual Research Report
  • [Book] 実験医学2014

    • Author(s)
      難波啓一, 加藤貴之, 藤井高志
    • Publisher
      羊土社
    • Description
      【発行確定】
    • Related Report
      2014 Annual Research Report
  • [Book] 化学と工業 Vol66-52013

    • Author(s)
      加藤貴之, 阮娟芳, 難波啓一
    • Total Pages
      68
    • Publisher
      日本化学会
    • Related Report
      2013 Annual Research Report
  • [Book] Encyclopedia of biophysics (Gordon C. K. Roberts, ed.)2013

    • Author(s)
      Minamino, T., Kato, T., Miyata, T., Namba, K.
    • Total Pages
      1690
    • Publisher
      Springer
    • Related Report
      2013 Annual Research Report
  • [Remarks] 日本電子YOKOGUSHI協働研究所<難波特任教授>

    • URL

      http://www.fbs.osaka-u.ac.jp/jpn/general/lab/02/result/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 報道 1. 寺原直矢、南野徹、難波啓一. 外環境を感知してエネルギー源を切り替えるエコロジカルなナノモーター, academist Journal, 2018年4月2日. 2. 寺原直矢、南野徹、難波啓一. 細菌のべん毛形成解明. 北國新聞,2018年4月26日. 3. 寺原直矢、井上由美、南野徹、難波啓一、今田勝巳. 阪大・金沢大・名大・九州工大、べん毛タンパク質輸送の交通整理の仕組みを解明-細菌感染症薬のスクリーニングへ応用. 日本経済新聞電子版, 2018年4月26日. 4. 寺原直矢, 井上由美, 難波啓一, 南野徹. 大阪大学、細菌べん毛タンパク質輸送の交通整理のしくみを解明. 日経バイオテクONLINE, 2018年5月1日. 5. 川本晃大. 東北大、「レプトスピラ」細菌の運動のしくみを解明. 日本経済新聞電子版, 2018年5月31日. 6. 加藤貴之, 牧野文信, 寺原直矢, 難波啓一. クライオ電顕で生体分子の3D構造解明が加速. 日経バイオテク, 2019年2月25日.

    • Related Report
      2018 Annual Research Report
  • [Remarks] プロトニックナノマシン研究室(難波特任教授)

    • URL

      http://www.fbs.osaka-u.ac.jp/jpn/general/lab/02/result/

    • Related Report
      2017 Annual Research Report
  • [Remarks] プロトニックナノマシン研究室<難波教授>

    • URL

      http://www.fbs.osaka-u.ac.jp/jpn/general/lab/02/result/

    • Related Report
      2016 Annual Research Report
  • [Remarks] フロトニックナノマシン研究室(難波教授)

    • URL

      http://www.fbs.osaka-u.ac.jp/jpn/general/lab/02/result/

    • Related Report
      2015 Annual Research Report
  • [Remarks] プロトニックナノマシン研究室〈難波教授〉

    • URL

      http://www.fbs.osaka-u.ac.jp/jpn/general/lab/02/result/

    • Related Report
      2014 Annual Research Report
  • [Remarks] プロトノニックナノマシン研究室〈難波教授〉

    • URL

      http://www.fbs.osaka-u.ac.jp/ipn/general/lab/02/result/

    • Related Report
      2013 Annual Research Report
  • [Funded Workshop] 大阪大学大学院生命機能研究科 FBS Seminar “Rethinking the genetic code as a triplet-of-triplets” by Prof. Kelly T. Hughes2018

    • Related Report
      2017 Annual Research Report
  • [Funded Workshop] 大阪大学大学院生命機能研究科 FBS Seminar “Dissecting a three-protein bacterial brain : E. coli's chemosensory array” by Prof. John Sandy Parkinson2018

    • Related Report
      2017 Annual Research Report
  • [Funded Workshop] 大阪大学大学院生命機能研究科 FBS Seminar “The increasing power of cryoEM for macromolecular structure determination” by Prof. Richard Henderson2018

    • Related Report
      2017 Annual Research Report

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Published: 2013-06-20   Modified: 2022-06-16  

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