| Co-Investigator(Kenkyū-buntansha) |
宝田 徹 国立研究開発法人理化学研究所, 前田バイオ工学研究室, 専任研究員 (30336010)
秋山 好嗣 東京理科大学, 基礎工学部教養(長万部), 講師 (40640842)
藤田 雅弘 国立研究開発法人理化学研究所, 前田バイオ工学研究室, 専任研究員 (50342845)
金山 直樹 信州大学, 総合工学系研究科(長野), 准教授(特定雇用) (80377811)
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| Budget Amount *help |
¥215,670,000 (Direct Cost: ¥165,900,000、Indirect Cost: ¥49,770,000)
Fiscal Year 2017: ¥35,490,000 (Direct Cost: ¥27,300,000、Indirect Cost: ¥8,190,000)
Fiscal Year 2016: ¥38,610,000 (Direct Cost: ¥29,700,000、Indirect Cost: ¥8,910,000)
Fiscal Year 2015: ¥44,590,000 (Direct Cost: ¥34,300,000、Indirect Cost: ¥10,290,000)
Fiscal Year 2014: ¥72,930,000 (Direct Cost: ¥56,100,000、Indirect Cost: ¥16,830,000)
Fiscal Year 2013: ¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
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| Outline of Final Research Achievements |
Applications of unique colloidal behaviors of nanoparticles densely modified with DNA were successfully broadened. First, linear assemblies of fully matched double-stranded (ds) DNA-modified nanoparticles were spontaneously folded into an island-like structure to readily afford two-dimensional nanoparticle arrays on substrate surfaces. Second, nanogels covered with DNA were constructed to be used as a drug delivery carrier that released model payloads in a thermoresponsive manner. Third, force-distance curve analyses between dsDNA layers using colloid probe-AFM revealed that the outermost complementary dsDNA layers attract each other at a high NaCl concentration. Finally, gene mutation assays and discovery methods of DNA-binding drug candidates were developed using the unique DNA-surface property. All results indicate that DNA molecules are unique surface-modifiers that are capable of controlling interactions between materials surfaces.
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