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Elucidation of molecular mechanisms of how piRNAs maintain the germline genome integrity from invasive mobile elements

Research Project

Project/Area Number 25221101
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Molecular biology
Research InstitutionThe University of Tokyo

Principal Investigator

Siomi Mikiko  東京大学, 大学院理学系研究科(理学部), 教授 (20322745)

Co-Investigator(Renkei-kenkyūsha) ISHIZU Hirotsugu  慶應義塾大学, 医学部, 助教 (40574588)
Project Period (FY) 2013-05-31 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥208,390,000 (Direct Cost: ¥160,300,000、Indirect Cost: ¥48,090,000)
Fiscal Year 2017: ¥37,440,000 (Direct Cost: ¥28,800,000、Indirect Cost: ¥8,640,000)
Fiscal Year 2016: ¥37,440,000 (Direct Cost: ¥28,800,000、Indirect Cost: ¥8,640,000)
Fiscal Year 2015: ¥41,210,000 (Direct Cost: ¥31,700,000、Indirect Cost: ¥9,510,000)
Fiscal Year 2014: ¥40,820,000 (Direct Cost: ¥31,400,000、Indirect Cost: ¥9,420,000)
Fiscal Year 2013: ¥51,480,000 (Direct Cost: ¥39,600,000、Indirect Cost: ¥11,880,000)
KeywordsPIWI / piRNA / トランスポゾン / RNAサイレンシング / ショウジョウバエ
Outline of Final Research Achievements

piRNAs arm race with mobile transposons in the germline to maintain the genome integrity from injurious invasion of the transposable elements. However, the molecular mechanism underlying the piRNA pathway remains elusive. In this study supported by KIBAN-S, we focused on understanding [I] the mechanisms of piRNA biogenesis and [II] the mechanism of Piwi-piRISC-mediated transcriptional transposon silencing. Moreover, [III] we aimed to establish a gene silencing system based on artificial piRNAs ectopically expressed in ovarian somatic cells. We made a lot of progress in this study; for example, we found that Yb body formation occurs in a hierarchical manner and that Vasa is necessary for releasing RNAs from Siwi-piRISC upon cleavage. Knock out of l(3)mbt induced the ping-pong machinery that was otherwise undetected in OSCs. We also solved the crystal structure of Siwi-piRISC. Moreover, we identified the cis-element necessary for producing piRNAs and its partner trans-acting protein, Yb.

Assessment Rating
Verification Result (Rating)

A

Assessment Rating
Result (Rating)

A: Progress in the research is steadily towards the initial goal. Expected research results are expected.

Report

(9 results)
  • 2018 Research Progress Assessment (Verification Result) ( PDF )
  • 2017 Final Research Report ( PDF )
  • 2016 Annual Research Report   Abstract(Research Progress Assessment) ( PDF )   Research Progress Assessment (Result) ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Abstract ( PDF )   Annual Research Report
  • Research Products

    (61 results)

All 2016 2015 2014 2013 Other

All Journal Article (23 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 23 results,  Open Access: 20 results,  Acknowledgement Compliant: 8 results) Presentation (34 results) (of which Int'l Joint Research: 17 results,  Invited: 12 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Crystal Structure of Silkworm PIWI-Clade Argonaute Siwi Bound to piRNA..2016

    • Author(s)
      Matsumoto N, Nishimasu H, Sakakibara K, Nishida KM, Hirano T, Ishitani R, Siomi H, Siomi MC, Nureki O.
    • Journal Title

      Cell

      Volume: 167 Pages: 484-497

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Production of Small Non-coding RNAs from the flamenco Locus Is Regulated by the gypsy Retrotransposon of Drosophila melanogaster.2016

    • Author(s)
      Guida V, Cernilogar FM, Filograna A, De Gregorio R, Ishizu H, Siomi MC, Schotta G, Bellenchi GC, Andrenacci D.
    • Journal Title

      Genetics

      Volume: 204(2) Pages: 631-644

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells.2016

    • Author(s)
      Sumiyoshi T, Sato K, Yamamoto H, Iwasaki YW, Siomi H, Siomi MC.
    • Journal Title

      Genes Dev

      Volume: 30 Pages: 1617-1622

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Piwi Modulates Chromatin Accessibility by Regulating Multiple Factors Including Histone H1 to Repress Transposons2016

    • Author(s)
      Iwasaki YW, Murano K, Ishizu H, Shibuya A, Iyoda Y, Siomi MC, Siomi H, Saito K.
    • Journal Title

      Molecular Cell

      Volume: 63 Pages: 1-12

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inheritance of a Nuclear PIWI from Pluripotent Stem Cells by Somatic Descendants Ensures Differentiation by Silencing Transposons in Planarian2016

    • Author(s)
      Shibata N, Kashima M, Ishiko T, Nishimura O, Rouhana L, Misaki K, Yonemura S, Saito K, Siomi H, Siomi MC, Agata K
    • Journal Title

      Dev. Cell

      Volume: 9 Pages: 226-237

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tudor-domain containing proteins act to make the piRNA pathways more robust in Drosophila2015

    • Author(s)
      Sato K, Iwasaki YW, Siomi H, Siomi MC
    • Journal Title

      Fly

      Volume: 9 Issue: 2 Pages: 86-90

    • DOI

      10.1080/19336934.2015.1128599

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Immuno-Electron Microscopy and Electron Microscopic In Situ Hybridization for Visualizing piRNA Biogenesis Bodies in Drosophila Ovaries.2015

    • Author(s)
      Shibata S*, Murota Y, Yoshimura M, Nagai T, Okano H, Siomi MC*. (*co-corresponding author)
    • Journal Title

      Methods in Molecular Biology

      Volume: 1328 Pages: 163-178

    • DOI

      10.1007/978-1-4939-2851-4_12

    • ISBN
      9781493928507, 9781493928514
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Krimper Enforces an Antisense Bias on piRNA Pools by Binding AGO3 in the Drosophila Germline2015

    • Author(s)
      Sato K, Iwasaki YW (equal contribution), Shibuya A, Carninci P, Tsuchizawa Y, Ishizu H, Siomi MC, Siomi H
    • Journal Title

      Molecular Cell

      Volume: 59 Issue: 4 Pages: 553-563

    • DOI

      10.1016/j.molcel.2015.06.024

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] TAF11 assembles the RISC loading complex to enhance RNAi efficiency.2015

    • Author(s)
      Liang C, Wang Y, Murota Y, Liu X, Smith D, Siomi MC and Liu Q.
    • Journal Title

      Molecular Cell

      Volume: 59 Issue: 5 Pages: 807-818

    • DOI

      10.1016/j.molcel.2015.07.006

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Somatic Primary piRNA Biogenesis Driven by cis-Acting RNA Elements and trans-Acting Yb2015

    • Author(s)
      Ishizu H, Iwasaki YW, Hirakata S, Ozaki H, Iwasaki W, Siomi H, Siomi MC
    • Journal Title

      Cell Reports

      Volume: 12 Issue: 3 Pages: 429-440

    • DOI

      10.1016/j.celrep.2015.06.035

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Phased piRNAs tackle transposons2015

    • Author(s)
      Siomi, H., and Siomi, MC
    • Journal Title

      Science

      Volume: 348 Issue: 6236 Pages: 756-757

    • DOI

      10.1126/science.aab3004

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Crystal Structure and Activity of the Endoribonuclease Domain of the piRNA Pathway Factor Maelstrom2015

    • Author(s)
      Naoki Matsumoto, Kaoru Sato, Hiroshi Nishimasu, Yurika Namba, Kana Miyakubi, Naoshi Dohmae, Ryuichiro Ishitani, Haruhiko Siomi, Mikiko C. Siomi, Osamu Nureki
    • Journal Title

      Cell Reports

      Volume: 11 Issue: 3 Pages: 1-10

    • DOI

      10.1016/j.celrep.2015.03.030

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Functional and structural insights into the piRNA factor Maelstrom.2015

    • Author(s)
      Sato K and Siomi MC.
    • Journal Title

      FEBS Letters

      Volume: 589 Issue: 14 Pages: 1688-1693

    • DOI

      10.1016/j.febslet.2015.03.023

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] PIWI-interacting RNA: Its Biogenesis and Functions2015

    • Author(s)
      Iwasaki Y.W., Siomi M.C., Siomi H.
    • Journal Title

      Annual Review of Biochemistry

      Volume: 84 Issue: 1 Pages: 405-433

    • DOI

      10.1146/annurev-biochem-060614-034258

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate characteristics during testicular development.2015

    • Author(s)
      Yu-Ching Lin Z, Hirano T, Shibata S, Seki NM, Kitajima R, Sedohara A, Siomi MC, Sasaki E, Siomi H, Imamura M, Okano H.
    • Journal Title

      Dev Biol.

      Volume: - Issue: 1 Pages: 43-58

    • DOI

      10.1016/j.ydbio.2015.01.014

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Respective functions of two distinct Siwi complexes assembled during piRNA biogenesis in insect germ cells.2015

    • Author(s)
      Nishida, KM., Iwasaki, YW., Murota, Y., Nagao, A., Mannen, T., Kato, Y., Siomi, H., and Siomi, MC.
    • Journal Title

      Cell Reports

      Volume: 10 Issue: 2 Pages: 193-203

    • DOI

      10.1016/j.celrep.2014.12.013

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] PIWI-Interacting RNA: Its Biogenesis and Functions.2015

    • Author(s)
      Iwasaki YW, Siomi MC, Siomi H.
    • Journal Title

      Annu Rev Biochem.

      Volume: -

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] piRNA clusters and open chromatin structure.2014

    • Author(s)
      Yamanaka, S., Siomi, MC. and Siomi, H.
    • Journal Title

      Mobile DNA

      Volume: 5 Issue: 1 Pages: 22-22

    • DOI

      10.1186/1759-8753-5-22

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Yb Integrates piRNA Intermediates and Processing Factors into Perinuclear Bodies to Enhance piRISC Assembly.2014

    • Author(s)
      Murota Y, Ishizu H, Nakagawa S, Iwasaki YW, Shibata S, Kamatani MK, Saito K, Okano H, Siomi H, Siomi MC.
    • Journal Title

      Cell Rep.

      Volume: 8 Issue: 1 Pages: 103-113

    • DOI

      10.1016/j.celrep.2014.05.043

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Small RNA profiling and characterization of piRNA clusters in the adult testes of the common marmoset, a model primate.2014

    • Author(s)
      Hirano T, Iwasaki YW, Lin ZY, Imamura M, Seki NM, Sasaki E, Saito K, Okano H, Siomi MC, *Siomi H.
    • Journal Title

      RNA

      Volume: 20 Issue: 8 Pages: 1223-1237

    • DOI

      10.1261/rna.045310.114

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] DmGTSF1 is necessary for Piwi–piRISC-mediated transcriptional transposon silencing in the Drosophila ovary2013

    • Author(s)
      Ohtani H et al.
    • Journal Title

      Genes Dev.

      Volume: 27 Issue: 15 Pages: 1693-1705

    • DOI

      10.1101/gad.221150.113

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of R2D2, a cytoplasmic D2 body component, in the endogenous siRNA pathway in Drosophila.2013

    • Author(s)
      Nishida, KM.
    • Journal Title

      Molecular Cell

      Volume: 49 Issue: 4 Pages: 680-691

    • DOI

      10.1016/j.molcel.2012.12.024

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification of dFMR1-Containing Complexes Using Tandem Affinity Purification2013

    • Author(s)
      Miyoshi K, Ogino A, Siomi MC, Siomi H.
    • Journal Title

      Trinucleotide Repeat Protocols (Methods Mol Biol)

      Volume: 1010 Pages: 111-121

    • DOI

      10.1007/978-1-62703-411-1_8

    • ISBN
      9781627034104, 9781627034111
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] piRNA生合成過程における中間体RNAの解析2016

    • Author(s)
      荒川 耕平,石津 大嗣,塩見 美喜子
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県 横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] Molecular Mechanism Underlying piRNA Biogenesis2016

    • Author(s)
      Mikiko Siomi
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県 横浜市)
    • Year and Date
      2016-11-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] Eggless in Piwi-piRNA-mediated transcriptional silencing in Drosophila ovarian somatic cells2016

    • Author(s)
      Saori Suzuki, Kaoru Sato, Ryo Onishi, Mikiko C Siomi
    • Organizer
      CSHA conference on RNA Biology
    • Place of Presentation
      蘇州市(中国)
    • Year and Date
      2016-11-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genic piRNA biogenesis in Drosophila Ovarian Somatic Cells; its molecular mechanism and meaning2016

    • Author(s)
      Kohei Arakawa, Hirotsugu Ishizu, Mikiko C Siomi
    • Organizer
      CSHA conference on RNA Biology
    • Place of Presentation
      蘇州市(中国)
    • Year and Date
      2016-11-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] PIWI-interacting RNAs in animals2016

    • Author(s)
      Mikiko Siomi
    • Organizer
      CNAF 2016
    • Place of Presentation
      広州市(中国)
    • Year and Date
      2016-11-08
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Biogenesis of PIWI-interacting RNA2016

    • Author(s)
      Mikiko Siomi
    • Organizer
      Regulatory & non-Coding RNAs
    • Place of Presentation
      Long Island(USA)
    • Year and Date
      2016-08-24
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells2016

    • Author(s)
      Tetsutaro Sumiyoshi, Kaoru Sato, Hitomi Yamamoto, Yuka W. Iwasaki, Haruhiko Siomi, Mikiko C. Siomi
    • Organizer
      Regulatory & non-Coding RNAs
    • Place of Presentation
      Long Island(USA)
    • Year and Date
      2016-08-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] X-ray structure of Bombyx mori PIWI protein Siwi with piRNA2016

    • Author(s)
      Kazuhiro Sakakibara, Naoki Matsumoto, Hiroshi Nishimasu, Kazumichi Nishida, Haruhiko Siomi, Osamu Nureki, and Mikiko C Siomi
    • Organizer
      Regulatory & non-Coding RNAs
    • Place of Presentation
      Long Island(USA)
    • Year and Date
      2016-08-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] RNAサイレンシングと女性研究者のキャリアパス2016

    • Author(s)
      塩見 美喜子
    • Organizer
      慶應義塾大学医学部 平成28年度学生自主企画医学・薬学合同サマースクール
    • Place of Presentation
      慶應義塾大学(東京都 新宿区)
    • Year and Date
      2016-07-12
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Immunoisolation and component analysis of Piwi-piRISC functioning in transcriptional silencing in Drosophila ovarian somatic cells2016

    • Author(s)
      Ryo Onishi , Kaoru Sato, Mikiko C. Siomi
    • Organizer
      RNA2016
    • Place of Presentation
      国立京都国際会館(京都府 京都市)
    • Year and Date
      2016-05-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulatory mechanism underlying the nuclear localization of the Piwi-piRNA complex in ovarian somatic cells2016

    • Author(s)
      Ryu Yashiro, Yukiko Murota, Mikiko C. Siomi
    • Organizer
      RNA2016
    • Place of Presentation
      国立京都国際会館(京都府 京都市)
    • Year and Date
      2016-05-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of mitochondrial piRNA factors in Drosophila ovarian cells2016

    • Author(s)
      Haruna Yamashiro, Horoki Kawamura, Hirotsugu Ishizu, and Mikiko C. Siomi
    • Organizer
      RNA2016
    • Place of Presentation
      国立京都国際会館(京都府 京都市)
    • Year and Date
      2016-05-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional analysis of a piRNA factor BmQin in BmN4 cells2016

    • Author(s)
      Yumi Miyata, Kazumichi M. Nishida, Yuka W. Iwasaki, Haruhiko Siomi and Mikiko C. Siomi
    • Organizer
      RNA2016
    • Place of Presentation
      国立京都国際会館(京都府 京都市)
    • Year and Date
      2016-05-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Activation of the ping-pong cycle in Drosophila ovarian somatic cells2016

    • Author(s)
      Mikiko Siomi
    • Organizer
      EMBO Workshop
    • Place of Presentation
      Montpellier(France)
    • Year and Date
      2016-04-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] piRNA Biogenesis in Drosophila.2016

    • Author(s)
      Siomi MC.
    • Organizer
      Keystone Symposia 2016
    • Place of Presentation
      Keystone(USA)
    • Year and Date
      2016-01-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] piRNA生合成因子Zucchiniのミトコンドリア局在の意義2015

    • Author(s)
      山城はるな
    • Organizer
      RNAフロンティアミーティング2015
    • Place of Presentation
      タカミヤビレッジホテル樹林(山形県・山形市)
    • Year and Date
      2015-12-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] カイコpiRNA増幅機構因子BmAgo3から反応後に標的RNAを解離する因子の探索2015

    • Author(s)
      榊原和洋
    • Organizer
      RNAフロンティアミーティング2015
    • Place of Presentation
      タカミヤビレッジホテル樹林(山形県・山形市)
    • Year and Date
      2015-12-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] piRNA生合成因子Zucchiniのミトコンドリア局在の意義2015

    • Author(s)
      山城はるな
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートピアホテル(兵庫県・神戸市)
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] piRNA増幅機構ping-pong cycleに関わるVasa様タンパク質の同定と機能解析2015

    • Author(s)
      榊原和洋
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートピアホテル(兵庫県・神戸市)
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] Biogenesis of PIWI-interacting RNAs in Drosophila germline.2015

    • Author(s)
      塩見美喜子
    • Organizer
      第2回六甲医学研究会
    • Place of Presentation
      六甲山ホテル(兵庫県・神戸市)
    • Year and Date
      2015-10-30
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Elucidation of the primary piRNA pathway in Drosophila ovarian somatic cells2015

    • Author(s)
      Hirotsugu Ishizu.
    • Organizer
      EMBO/EMBL Symposia
    • Place of Presentation
      Heidelberg(Germany)
    • Year and Date
      2015-10-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] PIWI-intracting RNA in Drosophila; its biogenesis and function.2015

    • Author(s)
      塩見美喜子
    • Organizer
      Naito Conference
    • Place of Presentation
      ガトーキングダム札幌(北海道・札幌市)
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 生殖細胞におけるpiRNA生合成因子Maelstromの分子機能解析2015

    • Author(s)
      難波祐里香
    • Organizer
      第17回日本RNA学会年会
    • Place of Presentation
      ホテルライフォート札幌(北海道・札幌市)
    • Year and Date
      2015-07-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] カイコpiRNA生合成経路におけるBmZucの機能解析2015

    • Author(s)
      西田知訓
    • Organizer
      第17回日本RNA学会年会
    • Place of Presentation
      ホテルライフォート札幌(北海道・札幌市)
    • Year and Date
      2015-07-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] 人工piRNA発現システムを用いたpiRNA生合成機構の解析2015

    • Author(s)
      石津大嗣
    • Organizer
      第17回日本RNA学会年会
    • Place of Presentation
      ホテルライフォート札幌(北海道・札幌市)
    • Year and Date
      2015-07-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] PIWI-intracting RNA in Drosophila; its biogenesis and function.2015

    • Author(s)
      Siomi MC.
    • Organizer
      40th FEBS Congress
    • Place of Presentation
      Berlin(Germany)
    • Year and Date
      2015-07-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Primary piRNA biogenesis in Drosophila ovarian somatic cells.2015

    • Author(s)
      Siomi MC.
    • Organizer
      The World of Regulatory RNAs
    • Place of Presentation
      Tel Aviv(Israel)
    • Year and Date
      2015-05-07
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Maelstrom, an endonuclease necessary for both initiation and effector phases in the piRNA pathway.2015

    • Author(s)
      Siomi MC.
    • Organizer
      10th Microsymposium on small RNAs
    • Place of Presentation
      Vienna(Austria)
    • Year and Date
      2015-05-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Yb integrates piRNA intermediates and processing factors into perinuclear bodies to enhance piRISC assembly2014

    • Author(s)
      Yukiko Murota, Hirotsugu Ishizu, Shinichi Nakagawa, Yuka W. Iwasaki, Shinsuke Shibata, Miharu K. Kamatani, Kuniaki Saito, Hideyuki Okano, Haruhiko Siomi and Mikiko C. Siomi
    • Organizer
      JAJRNA Meeting 2014
    • Place of Presentation
      University of Technology Sydney(オーストラリア)
    • Year and Date
      2014-11-01 – 2014-11-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Biogenesis of PIWI-interacting RNAs in drosophila germline2014

    • Author(s)
      塩見美喜子
    • Organizer
      ComBio 2014
    • Place of Presentation
      National Convention Centre(オーストラリア)
    • Year and Date
      2014-09-27 – 2014-10-02
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Functions of Yb in primary piRNA biogenesis in Drosophila2014

    • Author(s)
      塩見美喜子
    • Organizer
      the Regulatory & Non-Coding RNAs
    • Place of Presentation
      Cold Spring Harbor Laboratory(アメリカ)
    • Year and Date
      2014-08-26 – 2014-08-31
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] piRNA生合成関連因子Maelstromの機能解析2014

    • Author(s)
      難波祐里香、佐藤薫、松本直樹、西増弘志、濡木理、塩見春彦、塩見美喜子
    • Organizer
      第16回日本RNA学会年会
    • Place of Presentation
      ウインクあいち(名古屋市)
    • Year and Date
      2014-07-23 – 2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] primary piRNA生合成に働くcis-elementの探索2014

    • Author(s)
      平形樹生、石津大嗣、塩見美喜子
    • Organizer
      第16回日本RNA学会年会
    • Place of Presentation
      ウインクあいち(名古屋市)
    • Year and Date
      2014-07-23 – 2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] YbによるpiRNA生合成因子/piRNA前駆体の制御機構2014

    • Author(s)
      室田友紀子、石津大嗣、中川真一、岩崎由香、芝田晋介、鎌谷太陽、齋藤都暁、岡野栄之、塩見春彦、塩見美喜子
    • Organizer
      第16回日本RNA学会年会
    • Place of Presentation
      ウインクあいち(名古屋市)
    • Year and Date
      2014-07-23 – 2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Book] PIWI-interacting RNAs: Methods and Protocols2014

    • Author(s)
      Mikiko C.Siomi(Editor)
    • Total Pages
      249
    • Publisher
      Springer
    • Related Report
      2013 Annual Research Report
  • [Remarks] 東京大学大学院理学系研究科 生物科学専攻 塩見研究室HP

    • URL

      http://www-siomilab.biochem.s.u-tokyo.ac.jp/index.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 研究室ホームページ

    • URL

      http://www-siomilab.biochem.s.u-tokyo.ac.jp/index.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 塩見研究室ホームページ

    • URL

      http://www-siomilab.biochem.s.u-tokyo.ac.jp/index.html

    • Related Report
      2014 Annual Research Report

URL: 

Published: 2013-06-20   Modified: 2019-07-29  

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