| Budget Amount *help |
¥42,900,000 (Direct Cost: ¥33,000,000、Indirect Cost: ¥9,900,000)
Fiscal Year 2015: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2014: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2013: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
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| Outline of Final Research Achievements |
In human cells, multiple units of a PCNA homo-trimer are simultaneously mono-ubiquitinated at K164, which could activate unidentified mechanisms other than Polh-mediated translesion synthesis to replicate damaged DNA. Human Polh contains three PCNA-interacting motifs and a ubiquitin-interacting domain, all of which are included in the regulation of Polh-mediated translesion synthesis in a cooperative and redundant manner. A deubiquitinating enzyme, USP1, plays a crucial role in the regulation of DNA replication-coupled translesion DNA synthesis past UV-induced DNA lesions. USP7 is also involved in the PCNA deubiquitination and suppresses oxidative-stress-induced mutagenesis.
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