Analysis of mechanisms to tolerate for DNA replication blockage at DNA lesions
| Project/Area Number |
25241011
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
Masuda Yuji 名古屋大学, 環境医学研究所, 准教授 (30273866)
Kanao Rie 名古屋大学, 環境医学研究所, 助教 (30542287)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥42,900,000 (Direct Cost: ¥33,000,000、Indirect Cost: ¥9,900,000)
Fiscal Year 2015: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2014: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2013: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
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| Keywords | DNA損傷 / 損傷乗り越え複製 / Polh / PCNA / 翻訳後修飾 / ゲノム / 放射線 / 癌 / DNAポリメラーゼ・イータ / ユビキチン化 |
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| Outline of Final Research Achievements |
In human cells, multiple units of a PCNA homo-trimer are simultaneously mono-ubiquitinated at K164, which could activate unidentified mechanisms other than Polh-mediated translesion synthesis to replicate damaged DNA. Human Polh contains three PCNA-interacting motifs and a ubiquitin-interacting domain, all of which are included in the regulation of Polh-mediated translesion synthesis in a cooperative and redundant manner. A deubiquitinating enzyme, USP1, plays a crucial role in the regulation of DNA replication-coupled translesion DNA synthesis past UV-induced DNA lesions. USP7 is also involved in the PCNA deubiquitination and suppresses oxidative-stress-induced mutagenesis.
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Report
(4 results)
Research Products
(59 results)
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[Journal Article] Guanine-5-carboxylcytosine base pairs mimic mismatches during DNA replication.2014
Author(s)
Shibutani T, Ito S, Toda M, Kanao R, Collins LB, Shibata M, Urabe M, Koseki H, Masuda Y, Swenberg JA, Masutani C, Hanaoka F, Iwai S, Kuraoka I
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Journal Title
Scientific Reports
Volume: 4
Issue: 1
Pages: 5220-5220
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Shingosine, a modulator of human translesion DNA polymerase activity.2014
Author(s)
Kamath-Loeb AS, Balakrishna S, Whittington D, Shen JC, Emond MJ, Okabe T, Masutani C, Hanaoka F, Nishimura S, Loeb LA
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Journal Title
J Biol Chem
Volume: 289
Issue: 31
Pages: 21663-21672
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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