姫野 龍太郎 国立研究開発法人理化学研究所, 情報基盤センター, センター長 (60342838)
茶野 徳宏 滋賀医科大学, 医学部, 准教授 (40346028)
舘田 英典 九州大学, 理学(系)研究科(研究院), 教授 (70216985)
大野 みずき 九州大学, 医学(系)研究科(研究院), 助教 (70380524)
|Budget Amount *help
¥42,510,000 (Direct Cost: ¥32,700,000、Indirect Cost: ¥9,810,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2014: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
Fiscal Year 2013: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
|Outline of Final Research Achievements
We originally developed a “complete outbreeding method,” with which we succeeded in accumulating more than 1000 spontaneous mutations to a standard mouse inbred strain just in a year. We comprehensively identified de novo germline mutations as well as predisposed genetic variations with extremely high precision, which should give a powerful tool for the genetic risk assessment like very low dose radiations. We also succeeded in tracing the origin of each identified mutations in each pedigree and found that many de novo mutations had arisen in a very early developmental stage.
In parallel, we analyzed the biological functions of newly induced mutations and identified and established many mutant strains encompassing human disease models. We also constructed an open platform to make all the dataset and results available to the community.