| Budget Amount *help |
¥35,880,000 (Direct Cost: ¥27,600,000、Indirect Cost: ¥8,280,000)
Fiscal Year 2015: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2014: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2013: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
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| Outline of Final Research Achievements |
We developed new techniques for micropatterning individual cells and cell colonies, which eventually allowing for artificial uniformization of mechanical stresses as well as functional responses of the cells. Specifically, we demonstrated that localizations of cell adhesions and cytoskeletons, as a marker of cellular functional response, can be controlled in specific portions within cells, thus realizing that variations in the cellular response are dramatically suppressed. We further designed a real-time imaging system to observe the response of such artificially controlled cells subjected to extracellular mechanical stimuli. The techniques developed here will be useful in sophisticated modulation of cellular responses to apply them to bioengineering fields including tissue engineering.
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