| Budget Amount *help |
¥46,540,000 (Direct Cost: ¥35,800,000、Indirect Cost: ¥10,740,000)
Fiscal Year 2015: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2014: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2013: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
|
|---|
| Outline of Final Research Achievements |
Cationized gelatin was prepared by a chemical introduction of spermine or ethylene diamine into gelatin. Cationized gelatin nanospheres incorporating plasmid DNA (pDNA) or small interfering RNA (siRNA) were formulated through a coacervation in the presence of pDNA or siRNA. When cultured with mesenchymal stem cells derived from the bone marrow, the cationized gelatin nanospheres were internalized into the cells, followed by the intracellular release of pDNA or siRNA. The biological activity of pDNA or siRNA was enhanced and the time period was prolonged to a significantly great extent compared with that of free pDNA or siRNA. Stem cells were cultured in a 3-demensional gelatin sponge of scaffold combined with the nanospheres incorporating pDNA or siRNA. The cells proliferation was promoted while the biological activity of pDNA and siRNA of cells was also enhanced. It is concluded that the intracellular release technology is promising to promote the biological function of stem cells.
|
