Development of poorly water-soluble drug encapsulated protein nanocapsule possessing cancer directivity and application to DDS
| Project/Area Number |
25242046
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
Inui Takashi 大阪府立大学, 生命環境科学研究科(系), 教授 (80352912)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIBASHI Osamu 大阪府立大学, 生命環境科学研究科, 准教授 (70293214)
KATAOKA Yosuke 理化学研究所, ライフサイエンス技術基盤研究センター, チームリーダー (40291033)
DOI Hisashi 理化学研究所, ライフサイエンス技術基盤研究センター, チームリーダー (00421818)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAJIMA Hidemitsu 大阪府立大学, 生命環境科学研究科, 准教授 (30405360)
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| Research Collaborator |
WADA Koichi
YAGI Naoto
KIDA Tatsuya
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥47,450,000 (Direct Cost: ¥36,500,000、Indirect Cost: ¥10,950,000)
Fiscal Year 2015: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2014: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2013: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
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| Keywords | DDS / 難水溶性薬剤 / タンパク質ナノカプセル / 癌指向性 / 生体内輸送蛋白質 / 薬物送達システム / 生体内輸送タンパク質 / ナノカプセル / リポカリンタンパク質 / リポカリン蛋白質 |
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| Outline of Final Research Achievements |
We introduced CRGDK peptide, a tumor recognition peptide, at a C-terminal region of lipocalin-type prostaglandin D synthase (L-PGDS). L-PGDS-CRGDK labeled with a fluorescent reagent Cyto 750 was administered intravenously to human prostate cancer cells-bearing nude mice, and the metabolic disposition of L-PGDS-CRGDK was investigated using in vivo imaging system, resulting that a transient accumulation of L-PGDS-CRGDK to the cancer tissues was observed after 30 min to 5 h of the administration. Futhermore, we generated L-PGDS-based redox sensitive protein capsules by introducing a disulfide bond into the upper part of the drug-binding cavity of L-PGDS. The disulfide bond in capsules was opened and closed in an oxidation-reduction environment, suggesting that the capsules could be used as a drug delivery vehicle with drug controlled-release property.
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Report
(4 results)
Research Products
(80 results)
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[Journal Article] Development of pH-independent drug release formulation using lipocalin-type prostaglandin D synthase.2016
Author(s)
Mizoguchi, M., Nakatsuji, M., Takano, J., Ishibashi, O., Wada, K., Inui, T.
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Journal Title
J. Pharm. Sci.
Volume: 掲載確定
Issue: 9
Pages: 1-8
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Novel Characteristics of Guanosine 5’-monophosphate 1 Reductase in Pathogenic2 Protozoa Trypanosoma brucei Distinct from Host Animal.2016
Author(s)
T. Bessho, T. Okada, C. Kimura, T. Shinohara, A. Tomiyama, A. Imamura, M. Kuwamura, K. Nishimura, K. Fujimori, .S. Shuto, O. Ishibashi, B. K. Kubata, T. Inui
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Journal Title
PLoS Negl. Trop. Dis
Volume: 10
Issue: 1
Pages: e0004339-e0004339
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Nuclear-translocated glyceraldehyde-3-phosphate dehydrogenase promotes poly(ADP-ribose) polymerase-1 activation during oxidative/nitrosative stress in stroke.2015
Author(s)
Nakajima H, Kubo T, Ihara H, Hikida T, Danjo T, Nakatsuji M, Shahani N, Itakura M, Ono Y, Azuma Y, Inui T, Kamiya A, Sawa A, Takeuchi T
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Journal Title
J. Biol. Chem.
Volume: VOL. 290
Issue: 23
Pages: 14493-14503
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Human Lipocalin-Type Prostaglandin D Synthase-Based Drug Delivery System for Poorly Water-Soluble Anti-Cancer Drug SN-38.2015
Author(s)
Nakatsuji, M., Inoue, H., Kohno, M., Saito, M., Tsuge, S., Shimizu, S., Ishida, A., Ishibashi, O., Inui, T.
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Journal Title
PLoS ONE
Volume: 10(11)
Issue: 11
Pages: e0142206-e0142206
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Glyceraldehyde-3-phosphate Dehydrogenase Aggregates Accelerate Amyloid-β Amyloidogenesis in Alzheimer Disease.2015
Author(s)
Itakura, M., Nakajima, H., Kubo, T., Semi, Y., Kume, S., Higashida, S., Kaneshige, A., Kuwamura, M., Harada, N., Kita, A., Azuma, Y. T., Yamaji, R., Inui, T., Takeuchi, T.
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Journal Title
J. Biol. Chem.
Volume: 290
Issue: 43
Pages: 26072-26087
DOI
Related Report
Peer Reviewed
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[Journal Article] A Toxin Isolated from Sarcocystis fayeri in Raw Horsemeat May Be Responsible for Food Poisoning2014
Author(s)
Kamata, Y., Saito, M.,Irikura, D.,Yahata,Y.,Ohnishi, T., Bessho,T.,Inui,T.,Watanabe,M.,Konoshi,Y.,
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Journal Title
Journal of Food Protection
Volume: 77
Issue: 5
Pages: 814-819
DOI
Related Report
Peer Reviewed
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[Journal Article] Characterization of the novel Trypanosoma brucei inosine 5'-monophosphate dehydrogenase.2013
Author(s)
Bessho T, Morii S, Kusumoto T, Shinohara T, Noda M, Uchiyama S, Shuto S, Nishimura S, Djikeng A, Duszenko M, Martin SK, Inui T, Kubata KB.
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Journal Title
Parasitology
Volume: 140
Issue: 6
Pages: 735-745
DOI
Related Report
Peer Reviewed
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