| Budget Amount *help |
¥47,580,000 (Direct Cost: ¥36,600,000、Indirect Cost: ¥10,980,000)
Fiscal Year 2015: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2014: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2013: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
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| Outline of Final Research Achievements |
We showed the possibility that tyrosine 72 and histidine 78 was a catalyst residue of OAC from analysis and a variation introduction experiment of the complex X-rays crystal structure with the Oribe Thor acid which was Oribe Thor acid closure enzyme (OAC) derived from hemp and the product. Furthermore, We clarified what enzyme activity attenuated when bulky substituted the amino acid residue which formed a pentyl-binding pocket full of the hydrophobicity that we found in an active site of OAC for a higher bulky residue. Based on the X-ray crystallographic analysis about these variation enzymes, the capacity of the pentyl-binding pocket decreased by introduction of the variation, and a substrate became hard to be connected, it became clear to attenuate enzyme activity.
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