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A novel methodology for generation of molecular-targeting drugs via directed evolution in combinatorial libraries of conformationally constrained peptides

Research Project

Project/Area Number 25242068
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomolecular chemistry
Research InstitutionOsaka Prefecture University

Principal Investigator

Fujii Ikuo  大阪府立大学, 理学(系)研究科(研究院), 教授 (70189984)

Co-Investigator(Kenkyū-buntansha) TSUMURAYA Takeshi  大阪府立大学, 大学院理学系研究科, 准教授 (00372855)
藤原 大佑  大阪府立大学, 理学(系)研究科(研究院), 助教 (30611420)
Co-Investigator(Renkei-kenkyūsha) FUJIWARA Daisuke  大阪府立大学, 大学院理学系研究科, 助教 (30611420)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥47,320,000 (Direct Cost: ¥36,400,000、Indirect Cost: ¥10,920,000)
Fiscal Year 2015: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2014: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2013: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Keywords分子標的医薬品 / ペプチド / 進化分子工学 / ファージ表層提示法 / ヘリックス構造 / 生体分子 / 医療・福祉福祉 / ナノバイオ / 蛋白質 / 有機化学
Outline of Final Research Achievements

Despite intense research into the design of small ligands that target protein-protein interaction, a novel methodology for the rational design of such ligands remains an elusive goal. To design such ligands, our group has examined the directed evolution in a phage-displayed library of helix-loop-helix peptides. Screening of the library against targeted proteins provided bioactive peptides, whose rigid structures showed the spatial orientation of the pharmacophores, thus facilitating structure-based design of peptidomimetics. We constructed a phage-displayed library of the peptides and screened the library against several targeted proteins. The obtained peptide showed a strong binding affinity (Kd of 4 nM) , and a long half-life (>2 weeks) in mouse sera. In addition, the peptides provide structural information of the pharmacophores to facilitate structure-based design of peptidomimetics. Finally, we succeeded to generate biphenyl compounds binding to the targeted proteins.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (74 results)

All 2016 2015 2014 2013 Other

All Int'l Joint Research (1 results) Journal Article (22 results) (of which Peer Reviewed: 22 results,  Open Access: 3 results) Presentation (51 results) (of which Int'l Joint Research: 5 results,  Invited: 35 results)

  • [Int'l Joint Research] Sookmyung Women's Univerisity(韓国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] ‘Turn On/Off’ fluorescence probe for the screening of unactivated Bruton’s tyrosine kinase2015

    • Author(s)
      W. Kawahata, T. Asami, I. Fujii, and M. Sawa,
    • Journal Title

      Bioorganic & Medicinal Chemistry Letters

      Volume: 25 Pages: 2142-2145

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of an ultrasensitive immunoassay using affinity maturated antibodies for the measurement of rodent insulin2015

    • Author(s)
      Sunao Imai, Shoichi Naito, Tatsuya Takahashi, Akira Yamauchi, Etsuo Nakamura, Masaaki Sato, Yuuichi Mitsuda, Hiroyuki Takagi, Yoshito Numata, Ikuo Fujii, Shoji Yamane
    • Journal Title

      Analytical Biochemistry

      Volume: 473 Pages: 72-79

    • DOI

      10.1016/j.ab.2014.12.003

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a novel immunoassay specific for mouse intact proinsulin2015

    • Author(s)
      Sunao Imai, Tatsuya Takahashi, Shoichi Naito, Akira Yamauchi, Chihiro Okada, Yoshihide Notsu, Ikue Sakikawa, Michiyoshi Hatanaka, Takanori Iwasaki, Atsushi Morita, Ikuo Fujii, Shoji Yamane
    • Journal Title

      Analytical Biochemistry

      Volume: 484 Pages: 91-98

    • DOI

      10.1016/j.ab.2015.05.012

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microantibody-drug conjugates: synthesis and cellular uptake in HUVECs for vascular-targeting therapy,2015

    • Author(s)
      Masataka Michigami, Kentaro Takahashi, Zhengmao Ye, Ikuo Fujii,
    • Journal Title

      Peptide Science

      Volume: 2015 Pages: 43-44

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Modifying exosomes with arginine-rich peptides enhances cellular exosome uptake by inducing macropinocytosis2015

    • Author(s)
      Ikuhiko Nasase, Kosuke Noguchi, Nahoko Bailey Kobayashi, Tomoka Takahashi-Nakase, Tetsuhiko Yoshida, Ikuo Fujii, Shiro Futaki
    • Journal Title

      Peptide Science

      Volume: 2015 Pages: 83-84

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The cyclized helix-loop-helix peptide as a molecular scaffold for the design of intracellular protein-protein interaction inhibitors2015

    • Author(s)
      Daisuke Fujiwara, Hidekazu Kitada, Masahiro Oguri, Toshio Nishihara, Masataka Michigami, Kazunori Shiraishi, Eiji Yuba, Ikuhiko Nakase, Seiji Kodama, Kenji Kono, Sihyun Ham, Ikuo Fujii
    • Journal Title

      Peptide Science

      Volume: 2015 Pages: 85-86

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Poly-arginine conjugated helix-loop-helix peptide as an inhibitor for p53-HDM2 Interaction2015

    • Author(s)
      Shunsuke Inaura, Hidekazu Kitada, Masataka Michigami, Daisuke Fujiwara, Ikuhiko Nakase, Ikuo Fujii
    • Journal Title

      Peptide Science

      Volume: 2015 Pages: 259-260

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemical Synthesis of Head-to-Tail Cyclized Anti-VEGF Microantibody2014

    • Author(s)
      K. Takahashi, M. Michigami, and I. Fujii
    • Journal Title

      Peptide Science

      Volume: 2014 Pages: 143-144

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Design of helix-loop-helix peptide inhibitor for p53-HDM2 interaction2014

    • Author(s)
      H. Kitada, M. Oguri, D. Fujiwara, I. Fujii
    • Journal Title

      Peptide Science

      Volume: 2014 Pages: 271-272

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tumor Growth Inhibition by Anti-VEGF Microantibody2014

    • Author(s)
      M. Michigami, Z. Ye, Y. Koezuka, and I. Fujii
    • Journal Title

      Peptide Science

      Volume: 2014 Pages: 273-274

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Design of Protein Kinase Inhibitors: Conjugation of ATP-Competitive Molecules with Kinase Surface-Targeted Peptides2014

    • Author(s)
      R. Takayama, D. Fujiwara, and I. Fujii
    • Journal Title

      Peptide Science

      Volume: 2014 Pages: 313-314

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of Anti-VEGF Neutralizing Microantibodies from Phage-displayed Peptide Library,2014

    • Author(s)
      M. Suzuki, M. Michigami, Z. Ye, and I. Fujii,
    • Journal Title

      Peptide Science

      Volume: 2014 Pages: 315-316

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Peptide-based immunoadsorbents: Molecular grafting of Ig/Fc-binding epitopes of Protein A onto a de novo-designedhelix-loop-helix peptide2014

    • Author(s)
      K. Kawabata , H. Nagai , N. Konishi , D. Fujiwara, R. Sasaki, T. Ichikawa, I. Fujii
    • Journal Title

      Bioorganic & Medicinal Chemistry

      Volume: 22 Issue: 6 Pages: 1845-1849

    • DOI

      10.1016/j.bmc.2014.01.053

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-throughput de novo screening of receptor agonists with an automated single-cell analysis and isolation system.2014

    • Author(s)
      Yoshimoto N, Tatematsu K, Iijima M, Niimi T, Maturana AD, Fujii I, Kondo A, Tanizawa K, Kuroda S
    • Journal Title

      Sci Rep.

      Volume: 4 Issue: 1 Pages: 4242-4242

    • DOI

      10.1038/srep04242

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Preparation of Anti-Ciguatoxin Monoclonal Antibodies Using Synthetic Haptens : Sandwich ELISA Detection of Ciguatoxins2014

    • Author(s)
      円 谷健, 藤井郁雄, 平間正博
    • Journal Title

      Journal of AOAC International

      Volume: 97巻 Issue: 2 Pages: 373-379

    • DOI

      10.5740/jaoacint.sgetsumuraya

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An automated system for high-throughput single cell-based breeding2013

    • Author(s)
      Nobuo Yoshimoto, Akiko Kida, Xu Jie, Masaya Kurokawa, Masumi Iijima, Tomoaki Niimi, Andrés D. Maturana, Itoshi Nikaido, Hiroki R. Ueda, Kenji Tatematsu, Katsuyuki Tanizawa, Akihiko Kondo, Ikuo Fujii, Shun'ichi Kuroda
    • Journal Title

      Scientific Reports

      Volume: 3 Issue: 1 Pages: 1191-1191

    • DOI

      10.1038/srep01191

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The crystal structure of multidrug-resistance regulator RamR with multiple drugs2013

    • Author(s)
      Yamasaki S, Nikaido E, Nakashima R, Sakurai K, Fujiwara D, Fujii I, Nishino K
    • Journal Title

      Nature Comunications

      Volume: 4 Issue: 1 Pages: 1-7

    • DOI

      10.1038/ncomms3078

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antibody-catalyzed decarboxylation and aldol reactions using a primary amine molecules as a functionalized small nonprotein components2013

    • Author(s)
      Fumihiro Ishikawa, Kouki Uno, Masao Nishikawa, Takeshi Tsumuraya, Ikuo Fujii
    • Journal Title

      Bioorg. Med. Chem.

      Volume: 21 Issue: 22 Pages: 7011-7017

    • DOI

      10.1016/j.bmc.2013.09.015

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemical Synthesis and Modification of Cyclic Helix-Loop-Helix Peptides by Click Chemistry and Intramolecular Bis-Thioether Linkage2013

    • Author(s)
      T. Nishihara, H. Kitada, D. Fujiwara, and I. Fuji
    • Journal Title

      Peptide Science

      Volume: 2013 Pages: 135-136

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemically Modified Phage-displayed Peptide Libraries: Tethering Biotin to the Displayed Peptides2013

    • Author(s)
      Y. Nakamura, D. Fujiwara, and I. Fujii
    • Journal Title

      Peptide Science

      Volume: 2013 Pages: 353-354

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phage Selection of Peptide “Microantibodies2013

    • Author(s)
      D. Fujiwara and I. Fujii
    • Journal Title

      Protocols in Chemical Biology

      Volume: 5 Issue: 3 Pages: 171-194

    • DOI

      10.1002/9780470559277.ch130039

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Directed Evolution of Hydrolytic Antibodies in Phage-displayed Combinatrial Libraries2013

    • Author(s)
      Takeshi Tsumuraya and Ikuo Fujii
    • Journal Title

      Chemistry Letters

      Volume: 43 Issue: 3 Pages: 272-280

    • DOI

      10.1246/cl.131220

    • NAID

      130004868025

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 次世代抗体医薬:進化分子工学による分子標的ペプチド「マイクロ抗体」の開発2016

    • Author(s)
      藤井郁雄
    • Organizer
      第5回バイオ・メディカル・フォーラム/第2回産学連携ヘルスケアナレッジセミナー
    • Place of Presentation
      大阪府立大学
    • Year and Date
      2016-02-19
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 次世代抗体医薬:進化分子工学による分子標的ペプチドの創出2016

    • Author(s)
      藤井郁雄
    • Organizer
      メディカルジャパン2017大阪
    • Place of Presentation
      インテックス大阪, 大阪府
    • Year and Date
      2016-02-15
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] “マイクロ抗体”進化分子工学による分子標的ペプチドの創出2015

    • Author(s)
      藤井郁雄
    • Organizer
      京都大学 学際融合教育研究推進センター 生理化学研究ユニット 第5回シンポジウム
    • Place of Presentation
      京都大学
    • Year and Date
      2015-12-25
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] “MicroAntibodies”: Generation of Molecular-targeting Peptides by Directed Evolution in Phage-displayed Libraries of Conformationally Constrained Peptides2015

    • Author(s)
      藤井郁雄
    • Organizer
      Peptide Engineering Meeting 7 (PEM-7)
    • Place of Presentation
      Pune, India,
    • Year and Date
      2015-12-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 中分子創薬:抗体様分子標的ペプチド“マイクロ抗体”の創出2015

    • Author(s)
      藤井郁雄
    • Organizer
      第33回メディシナルケミストリーシンポジウム
    • Place of Presentation
      幕張国際研修センター, 千葉県
    • Year and Date
      2015-11-25
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Structural and Functional Analysis of Antibody 7B9 Catalyzing Hydrolyses of p-Nitrobenzyl Esters2015

    • Author(s)
      Naoki Miyamoto, Miho Yoshimura, Nobutoshi Ito, Takeshi Tsumuraya and Ikuo Fujii
    • Organizer
      The 3rd OPU-TKU International Symposiumon Frontier Chemistry and Materials for the 21st Century
    • Place of Presentation
      Osaka Prefecture University
    • Year and Date
      2015-11-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] EFFECTIVE CELLULAR UPTAKE OF EXOSOMES BY MODIFICATION WITH ARGININE-RICH PEPTIDES2015

    • Author(s)
      Ikuhiko Nakase, Kosuke Noguchi, Ikuo Fujii, Shiroh Futaki
    • Organizer
      The 3rd OPU-TKU International Symposiumon Frontier Chemistry and Materials for the 21st Century
    • Place of Presentation
      Osaka Prefecture University
    • Year and Date
      2015-11-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Selection of Anti-VEGF Microantibodiesfrom Phage-displayed Peptide Library2015

    • Author(s)
      Miho Suzuki, Masataka Michigami, Zhengmao Ye, and Ikuo Fujii
    • Organizer
      The 3rd OPU-TKU International Symposiumon Frontier Chemistry and Materials for the 21st Century
    • Place of Presentation
      Osaka Prefecture University
    • Year and Date
      2015-11-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] CYCLIZED HELIX-LOOP-HELIX PEPTIDE AS A MOLECULAR SCAFFOLD FOR THE DESIGN OF INTRACELLULAR PROTEIN-PROTEIN INTERACTION INHIBITORS2015

    • Author(s)
      Daisuke Fujiwara, Hidekazu Kitada, Masahiro Oguri, Toshio Nishihara, Masataka Michigami, Kazunori Shiraishi, Eiji Yub, Ikuhiko Nakase, Seiji Kodama, Kenji Kono, Sihyun Ham, and Ikuo Fujii
    • Organizer
      第52回ペプチド討論会
    • Place of Presentation
      平塚市中央公民館, 神奈川県
    • Year and Date
      2015-11-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] MicroAntibody-Drug Conjugates: Synthesis and Cellular Uptake in HUVECs for Vascular-Targeting Therapy2015

    • Author(s)
      Masataka Michigami, Kentaro Takahashi, Zhegmao Ye, Ikuo Fujii
    • Organizer
      第52回ペプチド討論会
    • Place of Presentation
      平塚市中央公民館, 神奈川県
    • Year and Date
      2015-11-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] POLY-ARGININE CONJUGATED HELIX-LOOP-HELIX PEPTIDE AS A INHIBITOR FOR P53-HDM2 INTERACTION2015

    • Author(s)
      Shunsuke Inaura1, Hidekazu Kitada, Masataka Michigami, Daisuke Fujiwara, Ikuhiko Nakase, Ikuo Fujii
    • Organizer
      第52回ペプチド討論会
    • Place of Presentation
      平塚市中央公民館, 神奈川県
    • Year and Date
      2015-11-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] MODIFYING EXOSOMES WITH ARGININE-RICH PEPTIDES ENHANCES CELLULAR EXOSOME UPTAKE BY INDUCING MACROPINOCYTOSIS2015

    • Author(s)
      Ikuhiko Nakase, Kosuke Noguchi, Nahoko Bailey Kobayashi, Tomoka Takatani-Nakase, Tetsuhiko Yoshida, Ikuo Fujii, and Shiroh Futaki
    • Organizer
      第52回ペプチド討論会
    • Place of Presentation
      平塚市中央公民館, 神奈川県
    • Year and Date
      2015-11-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] 中分子創薬のすすめ:進化分子工学による分子標的ペプチド(マイクロ抗体)の創出2015

    • Author(s)
      藤井郁雄
    • Organizer
      平成27年度 後期(秋季)有機合成化学講習会
    • Place of Presentation
      日本薬学会長井記念館長井記念ホール, 東京都
    • Year and Date
      2015-11-16
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] マイクロ抗体:進化分子工学による抗体様分子標的ペプチドの創出2015

    • Author(s)
      藤井郁雄
    • Organizer
      第67回日本生物工学会大会シンポジウム
    • Place of Presentation
      城山ホテル, 鹿児島県
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 薬か毒か 麻薬が人類にもたらしたもの2015

    • Author(s)
      藤井郁雄
    • Organizer
      サイエンスカフェ伊丹
    • Place of Presentation
      伊丹市立生涯学習センター, 兵庫県
    • Year and Date
      2015-10-16
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] シガトキシンのサンドイッチELISAにおける検出感度の向上2015

    • Author(s)
      円谷健, 平間正博, 藤井郁雄
    • Organizer
      第9回バイオ関連化学シンポジウム
    • Place of Presentation
      熊本大学
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] 抗体酵素6D9の触媒活性の向上2015

    • Author(s)
      吉村美穂、武田祐輔、宮本尚樹、円谷健、藤井郁雄
    • Organizer
      第9回バイオ関連化学シンポジウム
    • Place of Presentation
      熊本大学
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] 新規アフィニティーマチュレーション法「急がば回れ法」による抗体酵素の機能向上2015

    • Author(s)
      宮本尚樹, 吉村美穂,円谷 健,伊藤 暢聡,藤井 郁雄
    • Organizer
      第9回バイオ関連化学シンポジウム
    • Place of Presentation
      熊本大学
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] Holoabzyme: Catalytic antibodies with antigen-combining sites for artificial catalytic components2015

    • Author(s)
      藤井郁雄
    • Organizer
      Advanced Industrial Biotechnology and Bioengineering for Sustainable Bioindustry
    • Place of Presentation
      Hohehot,China
    • Year and Date
      2015-07-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ポスト抗体医薬:中分子創薬のすすめ進化分子工学による分子標的ペプチド“マイクロ抗体”の創出2015

    • Author(s)
      藤井郁雄
    • Organizer
      シオノギ未来創薬セミナー
    • Place of Presentation
      北海道大学
    • Year and Date
      2015-06-11
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ポスト抗体医薬:抗体様分子標的ペプチドの創出2015

    • Author(s)
      藤井郁雄
    • Organizer
      第9 回 高度医療都市を創出する未来技術シンポジウム, 生命医用工学の新展開(1), “未来の医療、医薬品を支える新規技術創出と人材育成”
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-04-25
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ポスト抗体医薬:抗体様分子標的ペプチドの創薬研究2015

    • Author(s)
      藤井 郁雄
    • Organizer
      日本薬学会第135年会シンポジウム「中分子創薬研究のフロンティア」
    • Place of Presentation
      神戸学院大学,神戸市
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ポスト抗体医薬:進化分子工学による分子標的ペプチドの創出2015

    • Author(s)
      藤井 郁雄
    • Organizer
      第20回ペプチドフォーラム「生命分子・ペプチド機能に学ぶ医薬品」
    • Place of Presentation
      長浜バイオ大学,長浜市
    • Year and Date
      2015-03-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Post-antibody drugs: Generation of molecular-targeting peptides “MicroAntibodies” by phage- displayed libraries2015

    • Author(s)
      Ikuo Fujii
    • Organizer
      Workshop on Innovation and Pioneering Technology (WINTech) 2015:Innovation by Synergy of Computational& Synchrotron Radiation Sciences
    • Place of Presentation
      神戸大学 統合研究拠点コンベンションホール,神戸市
    • Year and Date
      2015-03-12
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] “MicroAntibodes”: Generation of Molecular-targeting Peptides by Directed Evolution in Phage-displayed Libraries of Conformationally Constrained Peptides2015

    • Author(s)
      Ikuo Fujii
    • Organizer
      IBC’s 7th Annual AsiaTIDES
    • Place of Presentation
      Hyatt Regency Osaka,大阪府
    • Year and Date
      2015-03-05 – 2015-03-08
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Post-antibody drugs: Generation of molecular-targeting peptides “MicroAntibodies” by phage-displayed libraries.2015

    • Author(s)
      Ikuo Fujii
    • Organizer
      The 8th International Symposium for Future Technology Creating Better Human Health and Society
    • Place of Presentation
      岡山大学, 岡山市
    • Year and Date
      2015-02-06
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] “MicroAntibodes”: Generation of Molecular-targeting Peptides by Directed Evolution in Conformationally Constrained Peptide libraries2014

    • Author(s)
      Daisuke Fujiwara, Ikuo Fujii
    • Organizer
      The 3rd Asian Chemical Biology Conference
    • Place of Presentation
      Ngee Ann Kongsi Auditorium, National University of Singapore,Singapore
    • Year and Date
      2014-12-15 – 2014-12-17
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] “MicroAntibodes”:Generation of Molecular-targeting Peptides by Directed Evolution in Phage-Displayed Libraries of Structured Peptides2014

    • Author(s)
      Ikuo Fujii
    • Organizer
      15th Tetrahedron Symposium
    • Place of Presentation
      Singapore Expo, Singapore
    • Year and Date
      2014-10-28 – 2014-10-31
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] マイクロ抗体:立体構造規制ペプチドライブラリー法による分子標的ペプチドの創出2014

    • Author(s)
      藤井 郁雄
    • Organizer
      第37回日本分子生物学会年会 セッション :人工デザインによる分子認識の最前線 ―Scaffoldを中心として―
    • Place of Presentation
      パシフィコ横浜,横浜市
    • Year and Date
      2014-10-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ホロ抗体酵素: 人工補酵素-結合部位をもつ次世代抗体酵素の設計と創出2014

    • Author(s)
      藤井 郁雄
    • Organizer
      第87回日本生化学会大会 セッションテーマ:次世代機能抗体
    • Place of Presentation
      京都国際会議場,京都市
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] “MicroAntibodes”:Generation of Molecular-targeting Peptides by Directed Evolution in Phage-Displayed Libraries of Structured Peptides2014

    • Author(s)
      Ikuo Fujii
    • Organizer
      International Forum on Green Integrative Biotechnology
    • Place of Presentation
      Tsinghua University, Beijing, China
    • Year and Date
      2014-10-12
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] “MicroAntibodes”: Generation of Molecular-targeting Peptides by Directed Evolution in Phage-Displayed Libraries of Structured Peptides2014

    • Author(s)
      Ikuo Fujii
    • Organizer
      Peptides in Paris Symposium 2014
    • Place of Presentation
      University of Cergy-Pontoise, Genevilliers, France
    • Year and Date
      2014-10-05 – 2014-10-08
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Generation of Molecular-Targeting Peptides by Phage-Displayed Libraries of Helix-Loop-Helix Peptides ”MicroAntibody”2014

    • Author(s)
      Ikuo Fujii
    • Organizer
      2nd TKU OPU 4rh TKU ECUST OPU KIST Internarional Symposium
    • Place of Presentation
      淡水大学,台湾
    • Year and Date
      2014-09-25 – 2014-09-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] マイクロ抗体:酵母1分子1細胞スクリーニングによる 分子標的ペプチドの創出2014

    • Author(s)
      藤原太佑,藤井郁雄
    • Organizer
      第 66 回日本生物工学会大会
    • Place of Presentation
      札幌コンベンションセンター,札幌
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Generation of Molecular-Targeting Peptides by Phage-Displayed Libraries of Helix-Loop-Helix Peptides ”MicroAntibody”2014

    • Author(s)
      Ikuo Fujii
    • Organizer
      15th Akabori Conference Japanese-German Symposium on Peptide Science
    • Place of Presentation
      Boppard, Germany
    • Year and Date
      2014-09-07 – 2014-09-10
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] MICROANTIBODIES:GENERATION OF MOLECULAR-TARGRTING PEPTIDES FOR VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) FROM CONFORMATIONALLY CONSTRAINED PEPTIDE LIBRARIES2014

    • Author(s)
      Masataka Michigami, Zhegmao Ye, Yasuhiko Koeduka, Ikuo Fujii
    • Organizer
      33rd Europearn Peptide Symposium
    • Place of Presentation
      Sofia, Bulgaria
    • Year and Date
      2014-08-31 – 2014-09-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] 次世代抗体医薬“マイクロ抗体” : 進化分子工学による 分子標的ペプチドの創出2014

    • Author(s)
      藤井 郁雄
    • Organizer
      第18回JBICバイオ関連基盤技術研究会
    • Place of Presentation
      TIME24ビル 10階 JBIC事務局,江東区,東京都
    • Year and Date
      2014-08-08
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ホロ酵素型抗体酵素はどのようにして反応を触媒するのか?2014

    • Author(s)
      円谷 健,武 綾香,多田俊治,山口亜佐子,藤井郁雄
    • Organizer
      第16回生命化学研究会
    • Place of Presentation
      熱海
    • Related Report
      2013 Annual Research Report
  • [Presentation] 次世代抗体医薬 “マイクロ抗体”:進化分子工学による分子標的ペプチドの創出2014

    • Author(s)
      藤井 郁雄
    • Organizer
      新適塾 - 「未来創薬への誘い」第25回会合
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Post-antibody Medicines “MicroAntibodies”: Generation of Molecular-targeting Peptides by Directed Evolution in Phage-displayed Libraries2013

    • Author(s)
      Ikuo Fujii
    • Organizer
      Korean Chemiscal Society 2013
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] PRODUCTION OF ANTI-CIGUATOXIN MONOCLONAL ANTIBODIES USING SYNTHETIC HAPTENS: SANDWICH ELISA DETECTION OF CIGUATOXINS2013

    • Author(s)
      Takeshi Tsumuraya, Takeshi Sato, Masahiro Hirama, Ikuo Fujii
    • Organizer
      MARINE AND FRESHWATER TOXINS ANALYSIS 4TH Joint Symposium & Task Force Meeting
    • Place of Presentation
      Baiona, Spain
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 次世代抗体医薬:分子進化工学に抗体様分子標的ペプチドのデザインと創出2013

    • Author(s)
      藤井 郁雄
    • Organizer
      第13回日本蛋白質化学会年会
    • Place of Presentation
      鳥取
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ホロ酵素型抗体酵素27C1の構造解析と反応機構2013

    • Author(s)
      円谷 健,武 綾香,多田俊治,山口亜佐子,藤井郁雄
    • Organizer
      第13回日本蛋白質化学会年会
    • Place of Presentation
      鳥取
    • Related Report
      2013 Annual Research Report
  • [Presentation] Molecular Basis for Transition-state Stabilization in Catalytic Antibodies2013

    • Author(s)
      Ikuo Fujii
    • Organizer
      有機合成化学協会中国四国支部 パネル討論会 「分子をつくる方法論」
    • Place of Presentation
      鳥取大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Post-antibody Medicines “MicroAntibodies”: Generation of Molecular-targeting Peptides by Directed Evolution2013

    • Author(s)
      Ikuo Fujii
    • Organizer
      10th Australian Peptide Conference
    • Place of Presentation
      Penang, Malaysia
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ホロ酵素型抗体酵素27C1の構造解析と触媒機構2013

    • Author(s)
      円谷 健,武 綾香,多田俊治,山口亜佐子,藤井郁雄
    • Organizer
      第7回バイオ関連化学シンポジウム
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Design of Molecular-targeting Peptides and Peptidomimetics Based on Directed Evolution in Phage-displayed Libraries of Conformationally Constrained Peptides2013

    • Author(s)
      Ikuo Fujii
    • Organizer
      7th International Symposium on Computational Mcthods in Toxicology and Pharmacology Integration Internet Resources
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Chemical Modification of Peptide Libraries: Tethering Small Organic Compounds to Peptides on Phage Surface2013

    • Author(s)
      Yusuke Nakamura, Daisuke Fujiwara, and Ikuo Fujii
    • Organizer
      APIPS 2013 4th Asia-Pacific International Peptide Symposium
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
  • [Presentation] CHEMICAL SYNTHESIS AND MODIFICATION OF CYCLIC HELIX-LOOP-HELIX PEPTIDES BY CLICK CHEMISTRY AND INTRAMOLECULAR BIS-THIOETHER LINKAGE2013

    • Author(s)
      Toshio Nishhara, Hidekazu Kitada, Daisuke Fujiwara, Ikuo Fujii
    • Organizer
      APIPS 2013 4th Asia-Pacific International Peptide Symposium
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
  • [Presentation] Post Antibody Medicines ”MicroAntibody”: Generation of Molecular-targeting Peptides by Directed Evolution2013

    • Author(s)
      Ikuo Fujii
    • Organizer
      7th International Symposium on Nanomedicine
    • Place of Presentation
      九州工業大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Post-antibody Medicines “MicroAntibodies”: Generation of Molecular-targeting Peptides by Directed Evolution in Phage-displayed Libraries2013

    • Author(s)
      Ikuo Fujii
    • Organizer
      The First OPU-TKU International Symposium on Frontier hemistry and Materials for the 21st Century
    • Place of Presentation
      大阪府立大学
    • Related Report
      2013 Annual Research Report
    • Invited

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Published: 2013-05-15   Modified: 2019-07-29  

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