Screening for Novel Natural Products from Symbiotic Bacteria of Marine Sponge, Halichondria Okadai
Project/Area Number |
25242069
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biomolecular chemistry
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Research Institution | Kanagawa University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥46,670,000 (Direct Cost: ¥35,900,000、Indirect Cost: ¥10,770,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2014: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2013: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
|
Keywords | 天然物 / クロイソカイメン / 生物オリジン / メタゲノム / フローサイトメトリー / SDS-PAGE / 免疫染色 / Symbiodinolide / 天然物化学 / ハリクロームA / 生合成 / 生合成経路 / インドール誘導体 / カイメン / 生物活性 / 共生細菌 / 難培養性 / 抗腫瘍性 |
Outline of Final Research Achievements |
We improved the metagenomic method by converting the library into BAC, shuttle vector. An analysis method of new natural products using SDS-PAGE analysis was constructed. In addition, we developed the origin search method for natural products combining immunostaining method using natural products as antigen and flow cytometry. We further advanced the structural analysis of Symbiodinolide. It is expected to elucidate the symbiotic system by determining the isolation structure of the novel compound by the improved metagenomic method and identifying the origin of these natural products.
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Report
(5 results)
Research Products
(111 results)
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[Journal Article] 3. Jolkinolide F, a cytotoxic diterpenoid from Euphorbia jolkinii.2013
Author(s)
Maru, N., Chikaraishi, N., Yokota, K., Kawazoe, Y., and Uemura, D.
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Journal Title
Chem. Lett.
Volume: 42
Issue: 7
Pages: 756-757
DOI
NAID
Related Report
Peer Reviewed
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[Presentation] 抗肥満薬リードを求めて2016
Author(s)
上村大輔
Organizer
136回日本薬学会年会
Place of Presentation
横浜(パシフィコ横浜 会議センター 3F)
Year and Date
2016-03-27
Related Report
Invited
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[Presentation] Chemical Biology Fantasia2016
Author(s)
Daisuke Uemura
Organizer
International Symposium on Natural Products for the Future 2016 Tokushima(ISNPF2016)
Place of Presentation
Tokushima Bunri University, Tokushima, Japan
Related Report
Int'l Joint Research / Invited
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[Presentation] Chemical Biology Fantasia2015
Author(s)
上村大輔
Organizer
The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem)
Place of Presentation
アメリカ ハワイ Hilton Hawaiian Village Waikiki Beach Resort Hotel
Year and Date
2015-12-15
Related Report
Int'l Joint Research / Invited
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[Presentation] 藻類由来の生物活性物質の探索2015
Author(s)
鈴木智也、矢部丈登、川添嘉徳、上村大輔
Organizer
新規素材探索研究会第14回セミナー
Place of Presentation
神奈川県 横浜市 富士ビューホテル
Year and Date
2015-06-05
Related Report
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[Presentation] 天然物化学の新展開2015
Author(s)
上村大輔
Organizer
公益財団法人 山田科学振興財団
Place of Presentation
東京(東京コンファレンスセンター有明)
Year and Date
2015-05-31
Related Report
Invited
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[Presentation] 抗肥満薬リードの提案2015
Author(s)
上村大輔
Organizer
第14回国際バイオテクノロジー展
Place of Presentation
東京(東京ビックサイト)
Year and Date
2015-05-13
Related Report
Invited
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[Presentation] Chemical Biology Fantasia2014
Author(s)
上村大輔
Organizer
Asian Biological Medicine Summit Forum in Hangzhou: The International Symposium on Natural Products Chemistry and Chemical Biology 2014 & ACBI 2014 Hangzhou Meeting (国際学会)
Place of Presentation
中国 杭州市 Hangzhou Shujiang Hotel
Year and Date
2014-11-22 – 2014-11-25
Related Report
Invited
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