| Project/Area Number |
25246017
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nano/Microsystems
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| Research Institution | Kanagawa Academy of Science and Technology |
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Principal Investigator |
Takeuchi Shoji 公益財団法人神奈川科学技術アカデミー, 人工細胞膜システムグループ, プロジェクトリーダー (90343110)
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| Co-Investigator(Kenkyū-buntansha) |
大崎 寿久 公益財団法人神奈川科学技術アカデミー, 人工細胞膜システムグループ, 研究員 (50533650)
神谷 厚輝 公益財団法人神奈川科学技術アカデミー, 人工細胞膜システムグループ, 研究員 (70612315)
川野 竜司 公益財団法人神奈川科学技術アカデミー, 人工細胞膜システムグループ, 研究員 (90401702)
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| Co-Investigator(Renkei-kenkyūsha) |
SATO Koji 大学共同利用機関法人自然科学研究機構, 岡崎統合バイオサイエンスセンター, 特任准教授 (20444101)
IDE Toru 岡山大学, 自然科学研究科, 教授 (60231148)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥45,630,000 (Direct Cost: ¥35,100,000、Indirect Cost: ¥10,530,000)
Fiscal Year 2016: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2015: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2014: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Fiscal Year 2013: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
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| Keywords | 膜タンパク質（イオンチャネル） / 薬剤スクリーニング / 脂質膜 / マイクロ・ナノデバイス |
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| Outline of Final Research Achievements |
In this study, we developed a high-throughput platform for analyses of ion channel functions. A cell membrane model was artificially reconstructed on a microchip, in which we optimized the efficiency and reproducibility of the membrane formation and integrated the number of the membrane on a single chip. Accordingly, we examined reconstitution techniques of ion channels into the membrane on the chip, and finally verified the developed platform using functional assays of drug-target ion channels. The results demonstrated the feasibility of our platform, especially for precise ion channel analyses with a single-molecule level. Based on the developed technologies, we continuously improve the performance of the platform, hoping for practical use in drug screening of ion channels.
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