DVD biosensor based on artificial intelligence

• Project/Area Number: 25246033
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General applied physics
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Awazu Koichi 国立研究開発法人産業技術総合研究所, 電子光技術研究部門, 部門付 (60356392)
• Co-Investigator(Kenkyū-buntansha): MURAKAWA Masahiro 国立研究開発法人産業技術総合研究所, 人工知能研究センター, 研究チーム長 (10358153) ; SHIMA Takayuki 国立研究開発法人産業技術総合研究所, 電子光技術研究部門, 主任研究員 (10371048) ; FUJIMAKI Makoto 国立研究開発法人産業技術総合研究所, 電子光技術研究部門, 研究グループ長 (10392656) ; NOSATO Hirokazu 国立研究開発法人産業技術総合研究所, 人工知能研究センター, 主任研究員 (40435764) ; SONODA Yoriko 国立研究開発法人産業技術総合研究所, 電子光技術研究部門, 主任研究員 (70357333) ; SAKANASHI Hidenori 国立研究開発法人産業技術総合研究所, 人工知能研究センター, 主任研究員 (80357102) ; TAKAHASHI Eiichi 国立研究開発法人産業技術総合研究所, 人工知能研究センター, 研究チーム付 (80357368) ; 金里 雅敏 独立行政法人産業技術総合研究所, 電子光技術研究部門, 上級主任研究員 (60356805)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥45,500,000 (Direct Cost: ¥35,000,000、Indirect Cost: ¥10,500,000); Fiscal Year 2015: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000); Fiscal Year 2014: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000); Fiscal Year 2013: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
• Keywords: DVD / センサー / 人工知能 / 錯体化学 / 情報科学 / 無機材料 / 分析化学 / 菌 / 光ディスク / HLAC / 金属錯体 / 光記録 / 情報技術 / 金属錯体発光体

Outline of Final Research Achievements

Ｗe used a DVD based optics. DVD disc was used as the substrate. An ITO layer, a PtOx layer, and another ITO layer were deposited on the substrate. Reflectance of the sample was controlled by adjusting the thickness of the ITO layers. The PtOx layer was used to record marks to identify the position on the disk. E. coli (JM109, Takara Bio Inc.) was mixed in a deionized water to make a test water sample. A droplet of the test water was placed on the disk and dried at room temperature. For the evaluation, we used an optical disk tester.
An image obtained by the disk tester was identical with an image obtained by an optical microscope. The results demonstrated that E. coli was successfully detected and imaged by the optical disk technology. We would like to emphasize that the method is very simple. As a next step, it is important to combine with an appropriate analyzing method to distinguish between the microorganism on target and other substances of a similar size.

Research Products

• [Journal Article] Optical disk based imaging system to be used optical microscopy (2016)
  • Author(s): Takayuki Shima, Makoto Fujimaki and Koichi Awazu
  • Journal Title: Japanese Journal of Applied Physics Volume: 1 Pages: 1-3
  • Peer Reviewed / Open Access
• [Journal Article] Detection and Two-Dimensional Imaging of Escherichia coli Attached to an Optical Disk (2013)
  • Author(s): 島隆之、Subash Gopinath、藤巻真、粟津浩一
  • Journal Title: JAPANESE JOURNAL OF APPLIED PHYSICS Volume: 52 Issue: 10R Pages: 108004-108004
  • DOI: 10.7567/jjap.52.108004
  • NAID: 40022766184
  • Peer Reviewed
• [Journal Article] (2013)
  • Author(s): Takayuki Shima, Makoto Fujimaki, Akihiro Yoshida, Subash C. B. Gopinath, Masashi Kuwahara, Yoshimichi Ohki and Koichi Awazu
  • Journal Title: Journal of Biomaterials and Nanobiotechnology Volume: 4巻 Issue: 02 Pages: 145-150
  • DOI: 10.4236/jbnb.2013.42020
  • Peer Reviewed
• [Presentation] Rapid detection of microorganisms in water using optical disk technology (2015)
  • Author(s): 粟津 浩一、島 隆之、藤巻 真、野里 博和、坂無 英徳、高橋 栄一、 村川 正宏、園田 与理子、金里 雅敏
  • Organizer: Water Crisis Symposium
  • Place of Presentation: 香川大学/香川
  • Year and Date: 2015-02-12