| Budget Amount *help |
¥43,550,000 (Direct Cost: ¥33,500,000、Indirect Cost: ¥10,050,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2013: ¥26,000,000 (Direct Cost: ¥20,000,000、Indirect Cost: ¥6,000,000)
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| Outline of Final Research Achievements |
We elucidated the initial events of photoreceptor proteins at the molecular level using femtosecond time domain Raman spectroscopy that has been graded up to the ultimate level. The data obtained from photoactive yellow protein showed ultrafast changes in the low-frequency Raman signals, indicating that the hydrogen bond between the chromophore and adjacent amino acid residue is weakened in 100 fs after photoabsorption. It was also shown that the chromophore retains its initial trans conformation during the excited state lifetime, whereas it has a distorted cis conformation in the first ground state intermediate. In addition, we developed a new method, two dimensional femtosecond Raman excitation profile spectroscopy, and succeeded in visualizing the structural evolution of excited state molecules in ultrafast photoisomerization.
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