Development of highly sensitive linear probe without stem structure
| Project/Area Number |
25248037
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
Asanuma Hiroyuki 名古屋大学, 工学(系)研究科(研究院), 教授 (20282577)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIYA Yukiko 名古屋大学, エコトピア科学研究所, 講師 (00527947)
KASHIDA Hiromu 名古屋大学, 大学院工学研究科, 准教授 (30452189)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥47,450,000 (Direct Cost: ¥36,500,000、Indirect Cost: ¥10,950,000)
Fiscal Year 2015: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Fiscal Year 2014: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Fiscal Year 2013: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000)
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| Keywords | DNA / PNA / 蛍光プローブ / mRNA / ストランドインベージョン / ペリレン / リニアプローブ / アントラキノン / pBR３２２ / インベーダー / DsRed / eGFP |
|---|
| Outline of Final Research Achievements |
We have designed new fluorescent probe termed linear probe that does not involve stem structure. In this project, we have successfully developed extremely sensitive linear probe involving both perylenes and anthraquinones as fluorophores and quenchers, respectively; signal-to-back ground ratio became as high as 1600. Due to the remarkable nuclease resistivity, newly designed linear probe could fluorescently detect mRNA in cell. We have further developed strand-invader by combining linear probe and PNA. For this purpose, we multiply introduced ethynylperylenes and anthraquinones into DNA for stable complexation with DNA and suppressed complexation with PNA. When linear probe was incubated double-stranded DNA involving target site in the presence of complementary PNA, we could observe fluorescent increase due to the strand invasion. Even PCR product could be fluorescently labelled in a strand-invading manner.
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Report
(4 results)
Research Products
(190 results)
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[Journal Article] Faint electric treatment-induced rapid endosomal escape for efficient delivery of functional macromolecules into the cytoplasm2016
Author(s)
Hasan, M., Nishimoto, A., Ohgita, T., Hama, S., Kashida, H., Asanuma, H., Kogure, K.
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Journal Title
J. Control Release
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Peer Reviewed
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[Presentation] 非常識”な非環状人工核酸2015
Author(s)
浅沼浩之
Organizer
第１回秩序化分子システム 仙台ワークショップ
Place of Presentation
ホテルニュー水戸屋、仙台
Year and Date
2015-07-11
Related Report
Invited
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[Presentation] Tracing the “fate” of siRNA2015
Author(s)
Yukiko Kamiya, Anna Ito, and Hiroyuki Asanuma
Organizer
The 3rd China-Japan Symposium on Nanomedicine
Place of Presentation
Beijing
Year and Date
2015-06-19
Related Report
Int'l Joint Research / Invited
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[Presentation] Intracellular Monitoring of siRNA by Conjugation of Fluorescent Probe2014
Author(s)
Yukiko Kamiya, Anna Ito, Hiroshi Ito, Masaaki Urushihara, Junya Takai, Taiga Fujii, Xingguo Liang, Hiromu Kashida, and Hiroyuki Asanuma
Organizer
XXI Round Table on Nucleosides, Nucleotides and Nucleic Acids
Place of Presentation
Poznań, Poland
Year and Date
2014-08-24 – 2014-08-28
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[Presentation] Development of Modified siRNA for Improvement of RNAi Activity and Monitoring in Cells2013
Author(s)
Yukiko Kamiya, Junya Takai, Masaaki Urushihara, Hiroshi Ito, Xingguo Liang, Anna Ito, Kenji Yoshida, Taiga Fujii, Hiromu Kashida, Hiroyuki Asanuma
Organizer
62nd SPSJ Annual Meeting,
Place of Presentation
Kyoto
Related Report
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