| Budget Amount *help |
¥47,450,000 (Direct Cost: ¥36,500,000、Indirect Cost: ¥10,950,000)
Fiscal Year 2015: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Fiscal Year 2014: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Fiscal Year 2013: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000)
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| Outline of Final Research Achievements |
We have designed new fluorescent probe termed linear probe that does not involve stem structure. In this project, we have successfully developed extremely sensitive linear probe involving both perylenes and anthraquinones as fluorophores and quenchers, respectively; signal-to-back ground ratio became as high as 1600. Due to the remarkable nuclease resistivity, newly designed linear probe could fluorescently detect mRNA in cell. We have further developed strand-invader by combining linear probe and PNA. For this purpose, we multiply introduced ethynylperylenes and anthraquinones into DNA for stable complexation with DNA and suppressed complexation with PNA. When linear probe was incubated double-stranded DNA involving target site in the presence of complementary PNA, we could observe fluorescent increase due to the strand invasion. Even PCR product could be fluorescently labelled in a strand-invading manner.
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