liver tissue reconstruction based on micro-nano biotransport

• Project/Area Number: 25249018
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thermal engineering
• Research Institution: Waseda University
• Principal Investigator: TANISHITA Kazuo 早稲田大学, ナノ・ライフ創新研究機構, 客員上級研究員(研究院客員教授) (10101776)
• Co-Investigator(Kenkyū-buntansha): 須藤 亮 慶應義塾大学, 理工学部, 准教授 (20407141) ; 関口 哲志 早稲田大学, 付置研究所, 教授 (70424819)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥44,720,000 (Direct Cost: ¥34,400,000、Indirect Cost: ¥10,320,000); Fiscal Year 2016: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2015: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000); Fiscal Year 2014: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000); Fiscal Year 2013: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
• Keywords: 生物・生体工学 / 熱工学 / 再生医学 / 細胞・組織 / マイクロ・ナノデバイス / 細胞 / マイクロデバイス

Outline of Final Research Achievements

To achieve the reconstructive liver tissue, we focused on the vascularization of 3 dimensional reconstructive hepatocytes tissue and found out the cellular self-organization process in the micro-nano environments. We employed the conventional layer model and microfluidics model to reveal the cellular behavior for the 3D reconstruction of vascular endothelial cell and hepatocytes. The vascularization is derived by the direction of concentration gradient of growth factors secreted with the activated hepatocytes or the mechanical stimulus of interstitial flow. It is great interest to recognize that the vascularization is stabilized by the pericyte and network of hepatocytes

Research Products

• [Journal Article] Development of an <i>ex vivo</i> Brain Lactic Acid Monitoring System (2017)
  • Author(s): S. Negishi, S. Kamada, R. Kajiwara, Y. Kaneko, D.H. Yoon, T. Sekiguchi, S. Shoji, H. Kudo
  • Journal Title: IEEJ Transactions on Sensors and Micromachines Volume: 137 Issue: 1 Pages: 38-43
  • DOI: 10.1541/ieejsmas.137.38
  • NAID: 130005188402
  • ISSN: 1341-8939, 1347-5525
  • Peer Reviewed / Open Access
• [Journal Article] Fabrication of type I collaggen microcarrier using a microfluidic 3D T-junction device and its application for the quantitative analysis of cell-ECM interaction (2016)
  • Author(s): Ryo Sudo
  • Journal Title: Biofabrication Volume: 8 Pages: 035014-035023
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Microfluidic Stamping on Sheath Flow (2016)
  • Author(s): 2.D.H. Yoon, D. Tanaka, T. Sekiguchi, S. Shoji
  • Journal Title: Small Volume: Vol. 12 Issue: 24 Pages: 32243228-32243228
  • DOI: 10.1002/smll.201600552
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Retention and Bandwidth Predictions by Fast Gradient Elution Chromatography Using a Pillar Array Column (2016)
  • Author(s): Y. Song, K. Takatsuki, T. Sekiguchi, T. Funatsu, S. Shoji, M. Tsunoda
  • Journal Title: CHROMATOGRAPHY Volume: 37 Issue: 3 Pages: 111-116
  • DOI: 10.15583/jpchrom.2016.008
  • NAID: 130005277408
  • ISSN: 1342-8284, 1348-3315
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Solute diffusion through fibrotic tissue formed around protective cage system for implantable devices (2015)
  • Author(s): Ryo Sudo
  • Journal Title: Journal of Biomedical Materials Research Part B: Applied Biomaterials Volume: 103 Pages: 1180-1187
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Adhesive Forces between A1 Domain of von Willebrand Factor and N-terminus Domain of Glycoprotein Ibα Measured by Atomic Force Microscopy (2015)
  • Author(s): Kazuo Tanishita, Ryo Sudo
  • Journal Title: Journal of Atherosclerosis and Thrombosis Volume: 22 Pages: 1091-1099
  • NAID: 130005101337
  • Peer Reviewed
• [Journal Article] Formation of Polymeric Hollow Microcapsules and Microlenses Using Gas-in-Organic-in-Water Droplets (2015)
  • Author(s): D.H. Yoon, K. Hasegawa, Y. Kaneko, T. Arakawa, J.S. Go, T. Sekiguchi, S. Shoji
  • Journal Title: Micromachines 2015 Volume: 6 Issue: 5 Pages: 622-633
  • DOI: 10.3390/mi6050622
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Solute diffusion through fibrotic tissue formed around protective cage system for implantable devices (2015)
  • Author(s): Gunawan S. Prihandana, Hikaru Ito, Kohei Tanimura, Hiroshi Yagi, Yuki Hori, Orhan Soykan, Ryo Sudo, Norihisa Miki.
  • Journal Title: Journal of Biomedical Materials Research Part B: Applied Biomaterials Volume: Epub ahead of print Issue: 6 Pages: 1180-1187
  • DOI: 10.1002/jbm.b.33298
  • Peer Reviewed
• [Journal Article] Selective Droplet Sampling Using a Minimum Number of Horizontal Pneumatic Actuators in a High Aspect Ratio and Highly Flexible PDMS Device (2014)
  • Author(s): D. H. Yoon, D. Wakui, A. Nakahara, T. Sekiguchi, S. Shoji
  • Journal Title: RSC Advances 2015 Volume: 5 Issue: 3 Pages: 2070-2074
  • DOI: 10.1039/c4ra11254g
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Multiscale tissue engineering for liver reconstruction (2014)
  • Author(s): Ryo Sudo
  • Journal Title: Organogenesis Volume: １０ Pages: 216-224
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Pial arteries respond earlier than penetrating arterioles to neural activation in the somatosensory cortex in awake mice exposed to chronic hypoxia: an additional mechanism to proximal integration signaling? (2014)
  • Author(s): Sekiguchi Y, Takuwa H, Kawaguchi H, Kikuchi T, Okada E, Kanno I, Ito H, Tomita Y, Itoh Y, Suzuki N, Sudo R, Tanishita K, Masamoto K.
  • Journal Title: J Cereb Blood Flow Metab. Volume: 34 Issue: 11 Pages: 1761-1770
  • DOI: 10.1038/jcbfm.2014.140
  • Peer Reviewed / Open Access
• [Journal Article] Droplet-Based Microfluidics for High-Throughput Screening of a Metagenomic Library for Isolation of Microbial Enzymes (2014)
  • Author(s): M. Hosokawa, Y. Hoshino, Y. Nishikawa, T. Hirose, D.H. Yoon, T. Mori, T. Sekiguchi, S. Shoji, H. Takeyama
  • Journal Title: Biosensors and Bioelectronics Volume: なし Pages: 379-385
  • DOI: 10.1016/j.bios.2014.08.059
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Active Microdroplet Merging by Hydrodynamic Flow Control Using a Pneumatic Actuator-Assisted Pillar Structure (2014)
  • Author(s): D.H. Yoon, A. Jamshaid, J. Ito, A. Nakahara, D. Tanaka, T. Akitsu, T. Sekiguchi, S. Shoji
  • Journal Title: Lab on a Chip Volume: 2014 Issue: 16 Pages: 3050-3055
  • DOI: 10.1039/c4lc00378k
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Real-Time Monitoring of Chemical Reaction in Microdroplet Using Fluorescence Specctroscopy (2014)
  • Author(s): T. Fukuda, N, Funaki, T. Kurabayashi, M. Suzuki, D.H. Yoon, A. Nakahara, T. Sekiguchi, S. Shoji
  • Journal Title: Sensors and Actuators B Volume: 203 Pages: 536-542
  • DOI: 10.1016/j.snb.2014.06.045
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Multiscale tissue engineering for liver reconstruction (2014)
  • Author(s): Sudo R
  • Journal Title: Organogenesis Volume: 10 Issue: 2 Pages: 216-224
  • DOI: 10.4161/org.27968
  • Peer Reviewed / Open Access
• [Journal Article] MEMS技術を応用した再生工学 (2014)
  • Author(s): 須藤亮
  • Journal Title: 日本機械学会誌 Volume: 117 Pages: 24-27
• [Journal Article] Endothelial progenitor cells promote directional three-dimensional endothelial network formation by secreting vascular endothelial growth factor. (2013)
  • Author(s): Y. Abe, Y. Ozaki, J. Kasuya, K. Yamamoto, J. Ando, R. Sudo, M. Ikeda and K. Tanishita
  • Journal Title: PLoS ONE Volume: 8(12) Issue: 12 Pages: 1-12
  • DOI: 10.1371/journal.pone.0082085
  • Peer Reviewed
• [Journal Article] The lutheran/basal cell adhesion molecule promotes tumor cell migration by modulating integrin-mediated cell attachment to laminin-511 protein (2013)
  • Author(s): Kikkawa Y, Ogawa T, Sudo R, Yamada Y, Katagiri F, Hozumi K, Nomizu M, Miner JH
  • Journal Title: J Biol Chem Volume: 288 Issue: 43 Pages: 30990-31001
  • DOI: 10.1074/jbc.m113.486456
  • Peer Reviewed
• [Journal Article] Layer-specific dilation of penetrating arteries induced by stimulation of the nucleus basalis of Meynert in the mouse frontal cortex (2013)
  • Author(s): Ryo Sudo, Kazuo Tanishita et al.
  • Journal Title: Journal of Cerebral Blood Flow & Metabolism Volume: 33 Pages: 1440-1447
  • Peer Reviewed
• [Journal Article] A three-dimensional microfluidic tumor cell migration assay to screen the effect of anti-migratory drugs and interstitial flow (2013)
  • Author(s): Ryo Sudo, Kazuo Tanishita et al.
  • Journal Title: Microfluidics and Nanofluidics Volume: 14 Pages: 969-981
  • Peer Reviewed
• [Journal Article] The stabilization effect of mesenchymal stem cells on the formation of microvascular networks in a microfluidic device (2013)
  • Author(s): Ryo Sudo, Kazuo Tanishita et al.
  • Journal Title: Journal of Biomechanical Science and Engineering Volume: 8 Pages: 114-128
  • NAID: 130003366817
  • Peer Reviewed
• [Journal Article] Steady and pulsatile shear stress induce differenct three-dimensional endothelial networks through pseudopodium formation (2013)
  • Author(s): Ryo Sudo, Kazuo Tanishita et al.
  • Journal Title: Journal of Biorheology Volume: 27 Pages: 38-48
  • Peer Reviewed
• [Journal Article] 流れ刺激による血管ネットワーク形成のイメージング解析 (2013)
  • Author(s): 須藤亮
  • Journal Title: 日本血栓止血学会誌 Volume: 24 Pages: 576-581
  • NAID: 130003393320
• [Presentation] 微小培養環境制御に基づく3次元組織工学と細胞バイオメカニクス (2017)
  • Author(s): 須藤亮
  • Organizer: 次世代バイオ・医療技術研究会
  • Place of Presentation: 東京大学生産技術研究所（東京都目黒区）
  • Year and Date: 2017-03-31
  • Invited
• [Presentation] Multiscale tissue engineering for liver reconstruction (2017)
  • Author(s): Ryo Sudo
  • Organizer: 1st International Symposium on Applied Abstraction and Integrated Design (AAID2017)
  • Place of Presentation: 慶應義塾大学日吉キャンパス来往舎（神奈川県横浜市）
  • Year and Date: 2017-03-09
  • Int'l Joint Research
• [Presentation] グリオーマ幹細胞の浸潤形態に与えるヒアルロン酸の影響 (2017)
  • Author(s): 須藤亮
  • Organizer: シンポジウム：細胞アッセイ技術の現状と将来
  • Place of Presentation: 東京大学生産技術研究所コンベンションホール（東京都目黒区）
  • Year and Date: 2017-01-31
• [Presentation] 肝細胞調製培養液を用いた血管化肝組織構築のための工学的アプローチ (2017)
  • Author(s): 須藤亮
  • Organizer: シンポジウム：細胞アッセイ技術の現状と将来
  • Place of Presentation: 東京大学生産技術研究所コンベンションホール（東京都目黒区）
  • Year and Date: 2017-01-31
• [Presentation] マイクロ流体デバイスにおける3次元NVU培養モデルの構築 (2017)
  • Author(s): 須藤亮
  • Organizer: Neurovascular Unit研究会2017
  • Place of Presentation: 慶應義塾大学医学部信濃町キャンパス（東京都新宿区）
  • Year and Date: 2017-01-28
• [Presentation] 血管内皮細胞-間葉系幹細胞3次元共培養モデルにおける血管リモデリングの調査 (2017)
  • Author(s): 須藤亮
  • Organizer: 日本機械学会 第29回バイオエンジニアリング講演会
  • Place of Presentation: 愛知県産業労働センター ウインクあいち（愛知県名古屋市）
  • Year and Date: 2017-01-19
• [Presentation] 血管内皮細胞と間葉系幹細胞の共培養による脱細胞化肝臓内の血管ネットワークの再構築 (2017)
  • Author(s): 須藤亮
  • Organizer: 日本機械学会 第29回バイオエンジニアリング講演会
  • Place of Presentation: 愛知県産業労働センター ウインクあいち（愛知県名古屋市）
  • Year and Date: 2017-01-19
• [Presentation] 肝細胞-コラーゲンマイクロゲルビーズ混成組織の血管化に向けた検討 (2017)
  • Author(s): 須藤亮
  • Organizer: 日本機械学会 第29回バイオエンジニアリング講演会
  • Place of Presentation: 愛知県産業労働センター ウインクあいち（愛知県名古屋市）
  • Year and Date: 2017-01-19
• [Presentation] 間質流がグリオーマ幹細胞の浸潤能力に与える影響とそのメカニズムの評価 (2017)
  • Author(s): 須藤亮
  • Organizer: 日本機械学会 第29回バイオエンジニアリング講演会
  • Place of Presentation: 愛知県産業労働センター ウインクあいち（愛知県名古屋市）
  • Year and Date: 2017-01-19
• [Presentation] Effect of endothelial cells on invasion of glioma initiating cells in a microfluidic platform (2016)
  • Author(s): Ryo Sudo
  • Organizer: IEEE EMBS Micro and Nanotechnology in Medicine Conference
  • Place of Presentation: ：Hilton Waikoloa Village, Waikoloa, Hawaii, USA
  • Year and Date: 2016-12-12
  • Int'l Joint Research
• [Presentation] Fifty-Second Analysis of Oxidative Stress Markers in Human Plasma Using Amide-Modified Liquid Chromatography Chip (2016)
  • Author(s): Tetsushi Sekiguchi
  • Organizer: The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2016)
  • Place of Presentation: Dublin, Ireland
  • Year and Date: 2016-10-09
  • Int'l Joint Research
• [Presentation] In Vitro Selection of Novel Peptide Agonists for Human Somatostatin Receptor Subtype-2 Using a Water-In-Oil Microdroplet Platform (2016)
  • Author(s): Tetsushi Sekiguchi
  • Organizer: The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2016)
  • Place of Presentation: Dublin, Ireland
  • Year and Date: 2016-10-09
  • Int'l Joint Research
• [Presentation] Complex Check-Like Cross-Sectional Flow Formation for Fiber-Shaped Materials (2016)
  • Author(s): Tetsushi Sekiguchi
  • Organizer: The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2016),
  • Place of Presentation: Dublin, Ireland
  • Year and Date: 2016-10-09
  • Int'l Joint Research
• [Presentation] 血管内皮細胞がグリオーマ幹細胞の浸潤に与える影響 (2016)
  • Author(s): 須藤亮
  • Organizer: 生体医工学シンポジウム2016
  • Place of Presentation: 旭川市大雪クリスタルホール国際会議場（北海道旭川市）
  • Year and Date: 2016-09-17
• [Presentation] 脱細胞化肝臓骨格における血管ネットワーク再構築プロセスの観察 (2016)
  • Author(s): 須藤亮
  • Organizer: 日本機械学会2016年度年次大会
  • Place of Presentation: 九州大学伊都キャンパス（福岡県福岡市）
  • Year and Date: 2016-09-11
• [Presentation] Fabrication Process of Fluidic Devices for Producing Fine Droplets (2016)
  • Author(s): Tetsushi Sekiguchi
  • Organizer: The 16th International Conference on Nanotechnology,
  • Place of Presentation: 仙台国際センター、宮城県仙台市
  • Year and Date: 2016-08-22
  • Int'l Joint Research
• [Presentation] Reconstruction of vascularized hepatocyte tissues by hepatocyte-conditioned medium in a microfluidic device (2016)
  • Author(s): Ryo Sudo
  • Organizer: 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)
  • Place of Presentation: Disney’s Contemporary Resort, Orlando, FL, USA
  • Year and Date: 2016-08-16
  • Int'l Joint Research
• [Presentation] マイクロ流体デバイスにおける肝細胞調製培養液を使用した血管化肝組織構築の試み (2016)
  • Author(s): 須藤亮
  • Organizer: 第23回肝細胞研究会
  • Place of Presentation: 大阪大学中之島センター（大阪府大阪市）
  • Year and Date: 2016-07-07
• [Presentation] Accessibility to neighboring cells modulates cell viability and lineage decision of oligodendrocyte precursor cells in a novel 3-dimensional culture system (2016)
  • Author(s): Ryo Sudo
  • Organizer: The American Society for Neural Therapy and Repair (ASNTR) 2016 conference
  • Place of Presentation: Sheraton Sand Key Resort, Clearwater Beach, FL, USA
  • Year and Date: 2016-04-28
  • Int'l Joint Research
• [Presentation] オンチップがんモデル (2016)
  • Author(s): 須藤亮
  • Organizer: 細胞アッセイ研究会シンポジウム 細胞アッセイ技術の現状と将来
  • Place of Presentation: 東京大学生産技術研究所コンベンションホール（東京都目黒区）
  • Year and Date: 2016-01-19
  • Invited
• [Presentation] グリオーマの浸潤プロセスにおける血管内皮細胞の影響 (2016)
  • Author(s): 須藤亮
  • Organizer: 日本機械学会 第28回バイオエンジニアリング講演会
  • Place of Presentation: 東京工業大学（東京都目黒区）
  • Year and Date: 2016-01-09
• [Presentation] Neurovascular Unit構築に向けた神経幹細胞と脳微小血管内皮細胞の共培養条件の検討 (2016)
  • Author(s): 須藤亮
  • Organizer: 日本機械学会 第28回バイオエンジニアリング講演会
  • Place of Presentation: 東京工業大学（東京都目黒区）
  • Year and Date: 2016-01-09
• [Presentation] Quantitative morphological study of cell-ECM interaction using ECM microbeads (2015)
  • Author(s): Ryo Sudo
  • Organizer: 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)
  • Place of Presentation: Hwabaek International Convention Center, Gyeongju, Korea
  • Year and Date: 2015-10-25
  • Int'l Joint Research
• [Presentation] The effect of interstitial flow on the invasion ability and morphology of glioma stem cells (2015)
  • Author(s): Ryo Sudo
  • Organizer: 8th Asian-Pacific Conference on Biomechanics
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2015-09-16
  • Int'l Joint Research
• [Presentation] マイクロ流体デバイスを利用した毛細血管網吻合条件の検討 (2015)
  • Author(s): 須藤亮
  • Organizer: 日本機械学会2015年度年次大会
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2015-09-13
• [Presentation] Preparation and culture of collagen microspheres as a scaffold for liver tissue engineering (2015)
  • Author(s): Ryo Sudo
  • Organizer: 2015 4th TERMIS (the Tissue Engineering & Regenerative Medicine International Society) World Congress
  • Place of Presentation: Boston Marriott Copley Place, Boston, USA
  • Year and Date: 2015-09-08
  • Int'l Joint Research
• [Presentation] Construction of stable capillary networks using a microfluidic device (2015)
  • Author(s): Ryo Sudo
  • Organizer: 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: Milano Conference Center, Milano, Italy
  • Year and Date: 2015-08-25
  • Int'l Joint Research / Invited
• [Presentation] The effects of cell arrangement and growth factor gradient on vessel diameter in a microfluidic angiogenesis model (2015)
  • Author(s): Ryo Sudo
  • Organizer: 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: Milano Conference Center, Milano, Italy
  • Year and Date: 2015-08-25
  • Int'l Joint Research
• [Presentation] Construction of capillary anastomosis in a microfluidic device (2015)
  • Author(s): Ryo Sudo
  • Organizer: 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: Milano Conference Center, Milano, Italy
  • Year and Date: 2015-08-25
  • Int'l Joint Research
• [Presentation] ：The evaluation of interactions between hepatocytes and vascular networks in microenvironments (2015)
  • Author(s): Ryo Sudo
  • Organizer: 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: Milano Conference Center, Milano, Italy
  • Year and Date: 2015-08-25
  • Int'l Joint Research
• [Presentation] The evaluation of glioma invasion ability under interstitial flow conditions (2015)
  • Author(s): Ryo Sudo
  • Organizer: 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: Milano Conference Center, Milano, Italy
  • Year and Date: 2015-08-25
  • Int'l Joint Research
• [Presentation] Capillary network formation by the co-culture of endothelial cells and mesenchymal stem cells in a microfluidic device (2015)
  • Author(s): Ryo Sudo
  • Organizer: 6th International Conference on Computational Methods (ICCM2015)
  • Place of Presentation: Pullman Hotel, Auckland, New Zealand
  • Year and Date: 2015-07-14
  • Int'l Joint Research / Invited
• [Presentation] Vascular tissue engineering with microfluidics technology (2015)
  • Author(s): Ryo Sudo
  • Organizer: 1st Japan-Korea Biomedical Technology Symposium
  • Place of Presentation: 東京農工大学（東京都小金井市）
  • Year and Date: 2015-06-12
  • Int'l Joint Research / Invited
• [Presentation] 積層型共培養およびマイクロ流体デバイスを用いた血管化三次元肝組織構築の試み (2015)
  • Author(s): 須藤亮
  • Organizer: 第22回 肝細胞研究会
  • Place of Presentation: 米子コンベンションセンター（鳥取県米子市）
  • Year and Date: 2015-06-04
  • Invited
• [Presentation] 血管化肝組織構築プロセスにおける肝細胞と血管伸長の定量的評価 (2015)
  • Author(s): 須藤亮
  • Organizer: 第22回 肝細胞研究会
  • Place of Presentation: 米子コンベンションセンター（鳥取県米子市）
  • Year and Date: 2015-06-04
• [Presentation] Construction of microvascular networks under controlled culture microenvironments (2015)
  • Author(s): Ryo Sudo
  • Organizer: 15th International Congress of Biorheology and 8th International Conference on Clinical Hemorheology (ISB-ISCH 2015)
  • Place of Presentation: Korea University, Seoul, Korea
  • Year and Date: 2015-05-24
  • Int'l Joint Research / Invited
• [Presentation] 血管新生における細胞配置と増殖因子の濃度勾配による血管径への影響 (2015)
  • Author(s): 須藤亮
  • Organizer: 第54回日本生体医工学会大会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-05-07
• [Presentation] マイクロ流体デバイスを利用した毛細血管網吻合条件の検討 (2015)
  • Author(s): 須藤亮
  • Organizer: 第54回日本生体医工学会大会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-05-07
• [Presentation] 微小培養環境における肝細胞供給因子と血管ネットワークの相互作用の定量的評価 (2015)
  • Author(s): 須藤亮
  • Organizer: 第54回日本生体医工学会大会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-05-07
• [Presentation] グリオーマ幹細胞の浸潤プロセスにおける間質流の影響の評価 (2015)
  • Author(s): 須藤亮
  • Organizer: 第54回日本生体医工学会大会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-05-07
• [Presentation] 血管形成開始における血管内皮細胞と間葉系幹細胞の相互作用の影響 (2015)
  • Author(s): 上森寛元、谷村耕平、須藤亮
  • Organizer: 第14回日本再生医療学会総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2015-03-19 – 2015-03-21
• [Presentation] ペリサイト付き毛細血管培養モデルからNVUモデル構築への展開 (2015)
  • Author(s): 須藤亮
  • Organizer: Neurovascular Unit研究会2015
  • Place of Presentation: 慶應義塾大学医学部信濃町キャンパス（東京都新宿区）
  • Year and Date: 2015-01-31
  • Invited
• [Presentation] マイクロ流体デバイスを用いたグリオーマ幹細胞の浸潤モデル (2015)
  • Author(s): 須藤亮、多木壮太郞
  • Organizer: シンポジウム「細胞アッセイ技術の現状と将来」
  • Place of Presentation: 東京大学生産技術研究所コンベンションホール（東京都目黒区）
  • Year and Date: 2015-01-13
• [Presentation] HUVEC-MSC interactions in the initiation of capillary network formation (2014)
  • Author(s): Hiroyuki Uwamori, Kohei Tanimura, and Ryo Sudo
  • Organizer: IEEE Engineering in Medicine & Biology Society (EMBC) Micro and nanotechnology in medicine conference (MNMC) 2014
  • Place of Presentation: Turtle Bay Resort (Oahu, HI, USA)
  • Year and Date: 2014-12-08 – 2014-12-12
• [Presentation] 間葉系幹細胞と血管内皮細胞の相互作用による血管形成の開始 (2014)
  • Author(s): 谷村耕平、上森寛元、須藤亮
  • Organizer: 日本機械学会 第27回バイオエンジニアリング講演会
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2014-12-08 – 2014-12-12
• [Presentation] Liver tissue engineering using microfluidic devices (2014)
  • Author(s): Ryo Sudo
  • Organizer: International Biomedical Engineering Conference (IBEC) 2014
  • Place of Presentation: Kimdaejung Convention Center (Gwangju, Korea)
  • Year and Date: 2014-11-20 – 2014-11-22
  • Invited
• [Presentation] Multiscale liver and vascular tissue engineering (2014)
  • Author(s): 須藤亮
  • Organizer: 17th Biomechanics Seminar
  • Place of Presentation: 京都大学再生医科学研究所（京都府京都市）
  • Year and Date: 2014-11-14
  • Invited
• [Presentation] Capillary formation under interstitial flow in a microfluidic device for liver tissue engineering (2014)
  • Author(s): Ryo Sudo, Yoshinori Abe, Saki Menjo, Kazuo Tanishita
  • Organizer: 2014 BMES (Biomedical Engineering Society) annual meeting
  • Place of Presentation: Henry B. Gonzalez Convention Center (San Antonio, TX, USA)
  • Year and Date: 2014-10-22 – 2014-10-25
• [Presentation] ：Comparing of invasion form between glioma stem cells and the differentiated cells in a microfluidic 3D culture system (2014)
  • Author(s): Sotaro Taki, Shingo Fujioka, Sampetrean Oltea, Hideyuki Saya, Ryo Sudo
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 医工薬連携によるOrgans-on-a-chipの開発 (2014)
  • Author(s): 須藤亮
  • Organizer: ４大学ナノ・マイクロファブリケーション・シンポジウム
  • Place of Presentation: 新川崎・創造のもり地区（神奈川県川崎市）
  • Year and Date: 2014-09-16
  • Invited
• [Presentation] マイクロ流体デバイスを用いた細胞及び増殖因子位置制御による毛細血管網再構築の検討 (2014)
  • Author(s): 上森寛元、谷村耕平、須藤亮
  • Organizer: 日本機械学会年次大会
  • Place of Presentation: 東京電機大学（東京都足立区）
  • Year and Date: 2014-09-07 – 2014-09-10
• [Presentation] Reconstruction of microvascular networks in a microfluidic device (2014)
  • Author(s): Ryo Sudo, Hiroyuki Uwamori, Kohei Tanimura, Yoshinori Abe, Kazuo Tanishita
  • Organizer: 4th Japan-Switzerland Workshop on Biomechanics
  • Place of Presentation: Shima Kanko Hotel The Classic (Shima, Mie, Japan)
  • Year and Date: 2014-09-01 – 2014-09-04
  • Invited
• [Presentation] Effect of hepatocytes on capillary morphogenesis in a three-channel microfluidic device (2014)
  • Author(s): Mohammad Ajoudanian, Kohei Yasuda, Ryo Sudo
  • Organizer: 7th World Congress of Biomechanics
  • Place of Presentation: John B. Hynes Veterans Memorial Convention Center (Boston, MA, USA)
  • Year and Date: 2014-07-06 – 2014-07-11
• [Presentation] Initiation effect of hepatocyte-endothelial cell co-culture on capillary morphogenesis in a microfluidic device (2014)
  • Author(s): Saki Menjo, Ryo Sudo
  • Organizer: 7th World Congress of Biomechanics
  • Place of Presentation: John B. Hynes Veterans Memorial Convention Center (Boston, MA, USA)
  • Year and Date: 2014-07-06 – 2014-07-11
• [Presentation] Evaluation of glioma invasion using glioma-initiating cells in a microfluidic device (2014)
  • Author(s): Sotaro Taki, Ryo Sudo
  • Organizer: 7th World Congress of Biomechanics
  • Place of Presentation: John B. Hynes Veterans Memorial Convention Center (Boston, MA, USA)
  • Year and Date: 2014-07-06 – 2014-07-11
• [Presentation] Effect of unidirectional VEGF supply on the formation of capillary networks with pericytes in a microfluidic device (2014)
  • Author(s): Hiroyuki Uwamori, Ryo Sudo
  • Organizer: 7th World Congress of Biomechanics
  • Place of Presentation: John B. Hynes Veterans Memorial Convention Center (Boston, MA, USA)
  • Year and Date: 2014-07-06 – 2014-07-11
• [Presentation] 微小培養環境制御による肝細胞複合組織の再構築 (2014)
  • Author(s): 須藤亮
  • Organizer: 第21回 肝細胞研究会
  • Place of Presentation: 東京医科歯科大学（東京都文京区）
  • Year and Date: 2014-06-27 – 2014-06-28
• [Presentation] マイクロ流体デバイスを用いたグリオーマ幹細胞の浸潤形態および遊走能力の定量的評価 (2014)
  • Author(s): 多木壮太郞、藤岡真悟、須藤亮
  • Organizer: 第37回日本バイオレオロジー学会年会
  • Place of Presentation: 大宮ソニックシティ（埼玉県さいたま市）
  • Year and Date: 2014-06-05 – 2014-06-06
• [Presentation] The effect of interstitial flow on capillary morphogenesis and its application for hepatocyte-endothelium co-culture in a microfluidic device (2014)
  • Author(s): Ryo Sudo, Saki Menjo, Yoshinori Abe, Kazuo Tanishita
  • Organizer: International Symposium on Mechanobiology 2014 (ISMB 2014)
  • Place of Presentation: Junko Fukutake Hall, Okayama University (Okayama, Japan)
  • Year and Date: 2014-05-20 – 2014-05-23
• [Presentation] The effect of interstitial flow on the formation of microvascular networks in a microfluidic device (2014)
  • Author(s): Ryo Sudo, Yoshinori Abe, Kohei Tanimura, Hiroyuki Uwamori, Kazuo Tanishita
  • Organizer: The 18th International Vascular Biology Meeting (IVBM 2014)
  • Place of Presentation: Miyakomesse (Kyoto, Japan)
  • Year and Date: 2014-04-14 – 2014-04-17
• [Presentation] 増殖因子濃度勾配によるHUVEC-MSC共培養における毛細血管網再構築の検討 (2014)
  • Author(s): 須藤亮
  • Organizer: 第13回 日本再生医療学会総会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
• [Presentation] HUVEC-MSC共培養による毛細血管再構築に与える間質流の影響 (2014)
  • Author(s): 須藤亮
  • Organizer: 第13回 日本再生医療学会総会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
• [Presentation] 間質流制御による三次元組織の再生 (2014)
  • Author(s): 須藤亮
  • Organizer: 第13回 日本再生医療学会総会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
• [Presentation] 多孔性薄膜を用いた幹細胞の三次元組織再生 (2014)
  • Author(s): 須藤亮
  • Organizer: 第13回 日本再生医療学会総会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
• [Presentation] 毛細血管ネットワークの再構築における間質流の影響 (2014)
  • Author(s): 須藤亮
  • Organizer: 日本機械学会 第26回 バイオエンジニアリング講演会
  • Place of Presentation: 東北大学片平キャンパス（宮城県仙台市）
• [Presentation] 間質流制御による3次元血管ネットワークの形成 (2013)
  • Author(s): 谷下一夫、須藤亮
  • Organizer: 第36回 日本バイオレオロジー学会年会
  • Place of Presentation: ：九州大学 西新プラザ（福岡県福岡市）
• [Presentation] Microvessel formation induced with mechanical stimuli (2013)
  • Author(s): Kazuo Tanishita, Ryo Sudo,
  • Organizer: 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: 大阪国際会議場（大阪府大阪市）
  • Invited
• [Presentation] A three-dimensional invasion assay for glioma-initiating cells in a microfluidic device (2013)
  • Author(s): Ryo Sudo
  • Organizer: 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: 大阪国際会議場（大阪府大阪市）
• [Presentation] Effect of interstitial flow on reconstruction of capillary networks in HUVEC-MSC coculture (2013)
  • Author(s): Ryo Sudo
  • Organizer: 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: 大阪国際会議場（大阪府大阪市）
• [Presentation] Reconstruction of capillary networks in HUVEC-MSC coculture cultured in static/flow conditions in a microfluidic platform (2013)
  • Author(s): Ryo Sudo
  • Organizer: The 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)
  • Place of Presentation: Freiburg, Germany
• [Presentation] A microfluidic invasion assay for glioma-initiating cells in three-dimensional culture (2013)
  • Author(s): Ryo Sudo
  • Organizer: The 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)
  • Place of Presentation: Freiburg, Germany
• [Book] 細胞のマルチスケールメカノバイオロジー (2017)
  • Author(s): 須藤亮、谷下一夫
  • Total Pages: 31
  • Publisher: 森北出版
• [Book] Vascular Engineering (2016)
  • Author(s): Ryo Sudo, Kazuo Tanishita
  • Total Pages: 35
  • Publisher: Springer
• [Book] Vascular Engineering (2016)
  • Author(s): Kazuo Tanishita, Ryo Sudo
  • Total Pages: 401
  • Publisher: Springer
• [Remarks] Sudo Lab
  • URL: http://www.sudo.sd.keio.ac.jp/