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Glial dysfunction causes age-related memory impairment in Drosophila

Research Project

Project/Area Number 25250010
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionTokyo Metropolitan Institute of Medical Science

Principal Investigator

SAITOE Minoru  公益財団法人東京都医学総合研究所, 認知症・高次脳機能研究分野, 基盤技術研究センター長 (50261839)

Co-Investigator(Renkei-kenkyūsha) MIYASHITA Tomoyuki  公益財団法人東京都医学総合研究所, 認知症・高次脳機能研究分野, 主席研究員 (70270668)
UENO Kohei  公益財団法人東京都医学総合研究所, 認知症・高次脳機能研究分野, 副参事研究員 (40332556)
MATSUNO Motomi  公益財団法人東京都医学総合研究所, 認知症・高次脳機能研究分野, 主席研究員 (90392365)
HIRANO Yukinori  京都大学, 医学研究科メディカルイノベーションセンター, 准教授 (40580121)
NAGANOS Shintaro  公益財団法人東京都医学総合研究所, 認知症・高次脳機能研究分野, 主任研究員 (30631965)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥34,710,000 (Direct Cost: ¥26,700,000、Indirect Cost: ¥8,010,000)
Fiscal Year 2015: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2014: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2013: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Keywords老化 / 記憶 / ショウジョウバエ / D-セリン / 脳神経 / 学習記憶 / グリア / 脳・神経科学
Outline of Final Research Achievements

Age-related memory impairment (AMI) is a debilitating consequence of brain aging that can be suppressed in Drosophila by reducing PKA activity. However, the molecular mechanisms underlying AMI remain unclear. In the previous study, we found that age-associated increase in the activity of Drosophila pyruvate carboxylase (dPC), a mitochondrial anaplerotic enzyme, causes AMI. In this study, we clarify that dPC functions downstream of DC0-PK; DC0 mutations decrease expression of dPC and AMI in DC0 mutants is restored by expressing dPC in the glial cells whereas memory defects caused by overexpression of DC0 is suppressed by dPC mutations. Given that dPC activity suppresses serine racemase, enzyme that convert L-serine to D-serine, D-serine level was decreased upon aging in the wild type brain, while it was not altered in dPC/+ brain. Similarly, D-serine level was not changed upon aging in DC0/+ brain. Remarkably, AMI was significantly improved in wild-type by fed them with D-serine.

Report

(4 results)
  • 2016 Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (38 results)

All 2016 2015 2014 2013 Other

All Int'l Joint Research (2 results) Journal Article (13 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (19 results) (of which Int'l Joint Research: 2 results,  Invited: 5 results) Remarks (3 results) Funded Workshop (1 results)

  • [Int'l Joint Research] University of California, Barkeley(米国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Korea University(韓国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Learning defects in Drosophila growth restricted chico mutants are caused by attenuated adenylyl cyclase activity2016

    • Author(s)
      Naganos S, Ueno K, Horiuchi J, Saitoe M
    • Journal Title

      Molecular Brain

      Volume: Apr 6;9:37 Issue: 1 Pages: 1-10

    • DOI

      10.1186/s13041-016-0217-3

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Phosphorylation of TAR DNA-binding Protein of 43 kDa (TDP-43) by Truncated Casein Kinase 1δ Triggers Mislocalization and Accumulation of TDP-43.2016

    • Author(s)
      Nonaka T, Suzuki G, Tanaka Y, Kametani F, Hirai S, Okado H, Miyashita T, Saitoe M, Akiyama H, Masai H, Hasegawa M.
    • Journal Title

      The journal of Biological Chemistry

      Volume: 291 Issue: 11 Pages: 5473-83

    • DOI

      10.1074/jbc.m115.695379

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ショウジョウバエだから分かる学習記憶のメカニズム2016

    • Author(s)
      長野慎太郎、齊藤 実
    • Journal Title

      生体の科学

      Volume: 67 Pages: 13-16

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Long-Term Memory Formation in Drosophila Requires Training-Dependent Glial Transcription2015

    • Author(s)
      Matsuno M, Horiuchi J, Yuasa Y, Ofusa K, Miyashita T, Masuda T, and Saitoe M
    • Journal Title

      The Journal of Neuroscience

      Volume: 35 Issue: 14 Pages: 5557-5565

    • DOI

      10.1523/jneurosci.3865-14.2015

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 加齢による記憶力の低下とグリア細胞2015

    • Author(s)
      齊藤 実・堀内純二郎・山崎大介
    • Journal Title

      Clinical Neuroscience 月刊臨床神経科学

      Volume: 33 Pages: 1084-1085

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Glial dysfunction causes age-related memory impairment in Drosophila.2014

    • Author(s)
      Yamazaki D, Horiuchi J, Ueno K, Ueno T, Saeki S, Matsuno M, Naganos S, Miyashita T, Hirano Y, Nishikawa H, Taoka M, Yamauchi Y, Isobe T, Honda Y, Kodama T, Masuda T, Saitoe M
    • Journal Title

      Neuron

      Volume: 84 Issue: 4 Pages: 753-763

    • DOI

      10.1016/j.neuron.2014.09.039

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Hunger-driven modulation in brain functions2014

    • Author(s)
      Hirano Y, Saitoe M.
    • Journal Title

      Brain Nerve

      Volume: 66 Pages: 41-8

    • NAID

      40019947765

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Hunger-driven modulation in brain functions.2014

    • Author(s)
      Hirano Y, Saitoe M
    • Journal Title

      Brain Nerve

      Volume: 66 Pages: 41-48

    • NAID

      40019947765

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Hunger and memory; CRTC coordinates long-term memory with the physiological state, hunger.2013

    • Author(s)
      Hirano Y, Saitoe M.
    • Journal Title

      Commun Integr Biol.

      Volume: 6 Issue: 5 Pages: e25152-e25152

    • DOI

      10.4161/cib.25152

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fasting launches CRTC to facilitate long-term memory formation in Drosophila.2013

    • Author(s)
      Hirano, Y., Masuda, T., Naganos, S., Matsuno, M., Ueno, K., Miyashita, T., Horiuchi, J., Saitoe, M.
    • Journal Title

      Science

      Volume: 339 Issue: 6118 Pages: 443-446

    • DOI

      10.1126/science.1227170

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Perlecan regulates bidirectional Wnt signaling at the Drosophila neuromuscular junction2013

    • Author(s)
      Kamimura K., Ueno K., Nakagawa J., Hamada R., Saitoe M., and Maeda N
    • Journal Title

      J. Cell Biol

      Volume: 200 Issue: 2 Pages: 219-233

    • DOI

      10.1083/jcb.201207036

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-term enhancement of synaptic transmission between antennal lobe and mushroom body in cultured Drosophila brain.2013

    • Author(s)
      Ueno, K., Naganos, S., Hirano, Y., Horiuchi, J., Saitoe, M.
    • Journal Title

      J Physiol.

      Volume: 591 Issue: 1 Pages: 287-302

    • DOI

      10.1113/jphysiol.2012.242909

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 記憶改善に向けた新たな進展:軽度の空腹状態は長期記憶形成を促進する2013

    • Author(s)
      平野恭敬、齊藤 実
    • Journal Title

      細胞工学

      Volume: 32 Pages: 452-453

    • Related Report
      2013 Annual Research Report
  • [Presentation] 繰り返し学習の休息は長期記憶符号化神経細胞においてc-fos/creb転写サイクル生成のために必要である2015

    • Author(s)
      宮下知之、齊藤 実
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸コンベンションセンター(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Dopamine release is gated by coincidentally stimulated postsynaptic neurons to reinforce plasticity2015

    • Author(s)
      Ueno K, Saitoe M
    • Organizer
      Neuroscience 2015
    • Place of Presentation
      米国シカゴ
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Dopamine receptor activities regulate learning-dependent odor preference changes in Drosophila.2015

    • Author(s)
      Naganos S, Saitoe M
    • Organizer
      Neuroscience 2015
    • Place of Presentation
      米国シカゴ
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 新規On-demand型ドーパミン放出機構について2015

    • Author(s)
      齊藤 実
    • Organizer
      新学術領域「記憶ダイナミズム」数理シンポジウム
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Presynaptic dopamine release is gated by postsynaptic activity in Drosophila brain2015

    • Author(s)
      上野耕平、齊藤 実
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸コンベンションセンター(兵庫県神戸市)
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Neuron-glia interactions involved in memory formation and age-related memory impairment in Drosophila2015

    • Author(s)
      齊藤 実
    • Organizer
      新学術領域「グリアアセンブリ」夏の国際ワークショップ
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県岡崎市)
    • Year and Date
      2015-07-10
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 単離脳解析から見出されたドパミン放出の新機構2014

    • Author(s)
      上野耕平、齊藤 実
    • Organizer
      平成26年度包括脳ネットワーク冬のシンポジウム
    • Place of Presentation
      ホテル東京ガーデンパレス(東京都文京区)
    • Year and Date
      2014-12-12
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Dopamine release is gated by coincident stimulation of mushroom body neurons to establish plasticity2014

    • Author(s)
      上野耕平、齊藤 実
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Aging reduces glial protection from memory-induced cell death2014

    • Author(s)
      松野元美、堀内純二郎、齊藤 実
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Dopamine release is gated by coincident stimulation of postsynaptic mushroom body neurons to establish plasticity2014

    • Author(s)
      Saitoe M, Ueno K.
    • Organizer
      Learning and Memory: A Synthesis of Flies and Honeybees
    • Place of Presentation
      Janelia Farm Research Campus(USA)
    • Year and Date
      2014-09-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Glial regulation of age-related memory impairment in Drosophila2014

    • Author(s)
      Horiuchi J, Matsuno M, Masuda T, Saitoe M.
    • Organizer
      Learning and Memory: A Synthesis of Flies and Honeybees
    • Place of Presentation
      Janelia Farm Research Campus(USA)
    • Year and Date
      2014-09-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] The mechanisms of learning dependent odor preference changes following olfactory conditioning2014

    • Author(s)
      長野慎太郎、上野耕平、川端有紀、齊藤 実
    • Organizer
      日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 長期記憶形成に必要なグリア転写因子Repoによる遺伝子発現2013

    • Author(s)
      松野元美、齊藤 実
    • Organizer
      記憶回路研究会
    • Place of Presentation
      岡崎生理学研究所
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 神経可塑性に必要なドパミン放出はどのように決定されるのか2013

    • Author(s)
      上野耕平、堀内純二郎、齊藤 実
    • Organizer
      日本神経科学大会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] On-demand dopamine release, triggered by coincident stimuli, determines Drosophila mushroom body plasticity2013

    • Author(s)
      Ueno K, Horiuchi J, Saitoe M.
    • Organizer
      Cell Symposia: Networked Brain
    • Place of Presentation
      Sheraton San Diego Hotel and Marina, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Long-term memory formation requires neuron-glial signaling to induce glial protein synthesis.2013

    • Author(s)
      Matsuno M, Horiuchi J, Saitoe M.
    • Organizer
      Cell Symposia: Networked Brain
    • Place of Presentation
      Sheraton San Diego Hotel and Marina, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Age-related memory impairment in Drosophila is caused by an oxidative stress-independent impairment of glial neuromodulator release.2013

    • Author(s)
      Horiuchi J, Yamazaki D, Saitoe M.
    • Organizer
      Neurobiology of Drosophila
    • Place of Presentation
      Cold Spring Harbor Laboratory, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Coincident stimulation induces dopamine release required for long-term enhancement of Ca2+ influx in mushroom body neurons.2013

    • Author(s)
      Ueno K, Saitoe M.
    • Organizer
      Neurobiology of Drosophila
    • Place of Presentation
      Cold Spring Harbor Laboratory, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Olfactory aversive conditioning forms two distinct memory components in Drosophila.2013

    • Author(s)
      Naganos S, Ueno K, Horiuchi J, Saitoe M.
    • Organizer
      Neurobiology of Drosophila
    • Place of Presentation
      Cold Spring Harbor Laboratory, USA
    • Related Report
      2013 Annual Research Report
  • [Remarks] 東京都医学総合研究所学習記憶プロジェクト

    • URL

      http://www.igakuken.or.jp/memory/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 学習記憶プロジェクトHP

    • URL

      http://www.igakuken.or.jp/memory/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 東京都医学総合研究所学習記憶プロジェクト

    • URL

      http://www.igakuken.or.jp/memory/

    • Related Report
      2013 Annual Research Report
  • [Funded Workshop] Memory Dynamism2015

    • Place of Presentation
      京都大学稲盛ホール(京都府京都市)
    • Year and Date
      2015-11-06
    • Related Report
      2015 Annual Research Report

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Published: 2013-05-15   Modified: 2022-02-07  

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