|Budget Amount *help
¥45,890,000 (Direct Cost: ¥35,300,000、Indirect Cost: ¥10,590,000)
Fiscal Year 2016: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2015: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Fiscal Year 2014: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2013: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
|Outline of Final Research Achievements
We have introduced CRISPR/Cas9 system to generate genome edited mice. First, we prepared pCAG-EGxxFP plasmid to assess gRNA cleavage efficiency. Next, we injected gRNA/Cas9 expressing plasmids into pronucleus to generate genome edited mice. Transiently expressed gRNA/Cas9 complex digests the target locus and indels destroy gene function. Finally, we generated chimeric mice with genome-edited ES cells and analyzed gene function in mice. Our system can accelerate the reverse genetics in mice.