| Budget Amount *help |
¥44,070,000 (Direct Cost: ¥33,900,000、Indirect Cost: ¥10,170,000)
Fiscal Year 2015: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2014: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2013: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
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| Outline of Final Research Achievements |
This project focused on the molecular actions of Wnt5a, a representative ligand for the β-catenin-independent pathway, which has been implicated in various adult diseases including cancer. We obtained following results. 1) In polarized epithelial cells, Wnt5a and its receptor were trafficked to the basolateral region to regulate the formation of inner cavity of cysts by promoting cell-extracellular matrix attachment. 2) Wnt5a-dependent cancer cells invasion was depend on receptor-mediated endocytosis, but not and Wnt5a-dependent cancer cells proliferation was. 3) We developed a high throughput screening system to detect Wnt5a-dependent signal effectively and identified two chemical compounds for the inhibition of Wnt5a signaling. 4) Wnt5a signal enhanced interleukin-12 production in dendritic cells to promote Th1 immune-response in colorectal inflammation. These results suggest that Wnt5a signaling could be a novel therapeutic target for cancer and inflammation disease.
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