Studies for homologous recombination in chromatin

• Project/Area Number: 25250023
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genome biology
• Research Institution: Waseda University
• Principal Investigator: Kurumizaka Hitoshi 早稲田大学, 理工学術院, 教授 (80300870)
• Co-Investigator(Kenkyū-buntansha): 清水 光弘 明星大学, 理工学部, 教授 (80231364)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥45,890,000 (Direct Cost: ¥35,300,000、Indirect Cost: ¥10,590,000); Fiscal Year 2016: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000); Fiscal Year 2015: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000); Fiscal Year 2014: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000); Fiscal Year 2013: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
• Keywords: ゲノム維持修復 / ゲノム多様性 / ゲノム進化・再編 / 遺伝情報複製・再編 / ゲノム機能 / 活性発現の分子機構 / 染色体構築・機能・分配 / 生体高分子構造・機能 / 構造活性相関 / ゲノム / 遺伝子 / 癌 / 蛋白質 / 放射線

Outline of Final Research Achievements

The purpose of this study is to elucidate the molecular mechanism of homologous recombination reactions in the higher-ordered chromatin. In this study, we established the homologous pairing assay in nucleosomal template in vitro, and clarified the mechanism of homologous pairing reactions in in the higher-ordered chromatin. In addition, we found that the chromatin structure containing histone variants regulates the homologous pairing activities. Furthermore, we also found the functional differences of RAD51 and DMC1, which are key enzymes in homologous recombination.

Research Products

• [Int'l Joint Research] Carnegie Mellon University(米国)
• [Int'l Joint Research] フリードリッヒ・ミーシャ研究所(スイス)
• [Journal Article] Xeroderma pigmentosum group C protein interacts with histones: regulation by acetylated states of histone H3. (2017)
  • Author(s): Kakumu E
  • Journal Title: Genes Cells. Volume: 3 Issue: 3 Pages: 310-327
  • DOI: 10.1111/gtc.12479
  • Peer Reviewed / Open Access
• [Journal Article] Polymorphism of apyrimidinic DNA structures in the nucleosome. (2017)
  • Author(s): Osakabe, A., Arimura, Y., Matsumoto, S., Horikoshi, N., Sugasawa, K., Kurumizaka, H.
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 41783-41783
  • DOI: 10.1038/srep41783
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of the amino acid residues responsible for stable nucleosome formation by histone H3.Y. (2017)
  • Author(s): Kujirai T, Horikoshi N, Xie Y, Taguchi H, Kurumizaka H.
  • Journal Title: Nucleus. Volume: - Issue: 3 Pages: 1-10
  • DOI: 10.1080/19491034.2016.1277303
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] In vitro reconstitution and biochemical analyses of theSchizosaccharomyces pombe nucleosome. (2017)
  • Author(s): Masako Koyama, Wataru Nagakura, Hiroki Tanaka, Tomoya Kujirai, Yuji Chikashige, Tokuko Haraguchi, Yasushi Hiraoka, Hitoshi Kurumizaka.
  • Journal Title: Biochemical and biophysical research communications. Volume: 482 Issue: 4 Pages: 896-901
  • DOI: 10.1016/j.bbrc.2016.11.130
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] FANCI-FANCD2 stabilizes the RAD51-DNA complex by binding RAD51 and protects the 5′-DNA end. (2016)
  • Author(s): Sato K, Shimomuki M, Katsuki Y, Takahashi D, Kobayashi W, Ishiai M, Miyoshi H, Takata M, Kurumizaka H.
  • Journal Title: Nucleic Acids Res. Volume: 44 Pages: 10758-10711
  • NAID: 120006318854
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structure of the human DNA repair protein RAD52 containing surface mutations. (2016)
  • Author(s): Saotome, M., Saito, K., Onodera, K., Kurumizaka, H., and Kagawa, W.
  • Journal Title: Acta Cryst. F Volume: 72 Issue: 8 Pages: 598-603
  • DOI: 10.1107/s2053230x1601027x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Sequence-directed nucleosome-depletion is sufficient to activate transcription from a yeast core promoter in vivo (2016)
  • Author(s): Yuichi Ichikawa, Nobuyuki Morohashi, Nobuyuki Tomita, Aaron P. Mitchell, Hitoshi Kurumizaka, Mitsuhiro Shimizu
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 印刷中 Issue: 2 Pages: 57-62
  • DOI: 10.1016/j.bbrc.2016.05.063
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Structural and biochemical analyses of monoubiquitinated human histones H2B and H4 (2016)
  • Author(s): Machida S, Sekine S, Nishiyama Y, Horikoshi N, Kurumizaka H.
  • Journal Title: Open Biol. Volume: 6 Issue: 6 Pages: 160090-160090
  • DOI: 10.1098/rsob.160090
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Crystal structures of heterotypic nucleosomes containing histones H2A.Z and H2A (2016)
  • Author(s): Horikoshi N, Arimura Y, Taguchi H, Kurumizaka H.
  • Journal Title: Open Biol. Volume: 6 Issue: 6 Pages: 160127-160127
  • DOI: 10.1098/rsob.160127
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Chromatin architecture may dictate the target site for DMC1, but not for RAD51, during homologous pairing (2016)
  • Author(s): Kobayashi W., Takaku M., Machida S., Tachiwana H., Maehara K., Ohkawa Y. and Kurumizaka H
  • Journal Title: Scientific reports Volume: 6 Issue: 1 Pages: 24428-24428
  • DOI: 10.1038/srep24228
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Relaxed Chromatin Formation and Weak Suppression of Homologous Pairing by the Testis-Specific Linker Histone H1T. (2016)
  • Author(s): Machida S, Hayashida R, Takaku M, Fukuto A, Sun J, Kinomura A, Tashiro S, Kurumizaka H.
  • Journal Title: Biochemistry. Volume: 55 Issue: 4 Pages: 637-646
  • DOI: 10.1021/acs.biochem.5b01126
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Structural basis of pyrimidine-pyrimidone (6-4) photoproduct recognition by UV-DDB in the nucleosome. (2015)
  • Author(s): Osakabe, A., Tachiwana, H., Kagawa, W., Horikoshi, N., Matsumoto, S., Hasegawa, M., Matsumoto, N., Toga, T., Yamamoto, J., Hanaoka, F., Thoma, N.H., Sugasawa, K., Iwai, S., Kurumizaka, H.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 16330-16330
  • DOI: 10.1038/srep16330
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Influence of DNA methylation on positioning and DNA flexibility of nucleosomes with pericentric satellite DNA. (2015)
  • Author(s): Osakabe A, Adachi F, Arimura Y, Maehara K, Ohkawa Y, Kurumizaka H.
  • Journal Title: Open Biol. Volume: 5 Issue: 10 Pages: 150128-150128
  • DOI: 10.1098/rsob.150128
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Human FAN1 promotes strand incision in 5'-flapped DNA complexed with RPA. (2015)
  • Author(s): Takahashi D, Sato K, Hirayama E, Takata M, Kurumizaka H.
  • Journal Title: J Biochem Volume: 158 Issue: 3 Pages: 263-70
  • DOI: 10.1093/jb/mvv043
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Nucleosome organization and chromatin dynamics in telomeres (2015)
  • Author(s): Yuichi Ichikawa, Yoshifumi Nishimura, Hitoshi Kurumizaka, and Mitsuhiro Shimizu
  • Journal Title: Biomolecular Concepts Volume: 6 Issue: 1 Pages: 67-75
  • DOI: 10.1515/bmc-2014-0035
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Human tNASP promotes in vitro nucleosome assembly with histone H3.3. (2015)
  • Author(s): Kato, D., Osakabe, A., Tachiwana, H., Tanaka, H., and Kurumizaka, H.
  • Journal Title: Biochemistry Volume: 54 Issue: 5 Pages: 1171-1179
  • DOI: 10.1021/bi501307g
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Green fluorescent protein fused to the C terminus of RAD51 specifically interferes with secondary DNA binding by the RAD51-ssDNA complex (2014)
  • Author(s): Kobayashi, W., Sekine, S., Machida, S., and Kurumizaka, H.
  • Journal Title: Genes & Genetic Systems Volume: 89 Issue: 4 Pages: 169-179
  • DOI: 10.1266/ggs.89.169
  • NAID: 130004750050
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Defective FANCI Binding by a Fanconi Anemia-Related FANCD2 Mutant. (2014)
  • Author(s): Sato K, Ishiai M, Takata M, Kurumizaka H.
  • Journal Title: PLoS One Volume: 9 Issue: 12 Pages: e114752-e114752
  • DOI: 10.1371/journal.pone.0114752
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A method for evaluating nucleosome stability with a protein-binding fluorescent dye. (2014)
  • Author(s): Taguchi H, Horikoshi N, Arimura Y, Kurumizaka H.
  • Journal Title: Methods. Volume: 70 Issue: 2-3 Pages: 119-126
  • DOI: 10.1016/j.ymeth.2014.08.019
  • Peer Reviewed / Open Access
• [Journal Article] Expression and purification of human FANCI and FANCD2 using Escherichia coli cells. (2014)
  • Author(s): Daisuke Takahashi, Koichi Sato, Mayo Shimomuki, Minoru Takata, Hitoshi Kurumizaka
  • Journal Title: Protein Expression and Purification Volume: 103 Pages: 8-15
  • DOI: 10.1016/j.pep.2014.08.012
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Nap1 stimulates homologous recombination by RAD51 and RAD54 in higher-ordered chromatin containing histone H1. (2014)
  • Author(s): Machida, S., Takaku, M., Ikura, M., Sun, J., Suzuki, H., Kobayashi, W., Kinomura, A., Osakabe, A., Tachiwana, H., Horikoshi, Y., Fukuto, A., Matsuda, R., Ura, K., Tashiro, S., Ikura, T., and Kurumizaka, H.
  • Journal Title: Scientific Rep. Volume: 4 Issue: 1 Pages: 4863-4863
  • DOI: 10.1038/srep04863
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] FANCD2 Binds CtIP and Regulates DNA-End Resection during DNA Interstrand Crosslink Repair. (2014)
  • Author(s): Unno J
  • Journal Title: Cell Rep. Volume: 7 Issue: 4 Pages: 1039-1047
  • DOI: 10.1016/j.celrep.2014.04.005
  • Peer Reviewed / Open Access
• [Journal Article] Compensatory functions and interdependency of the DNA-binding domain of BRCA2 with the BRCA1-PALB2-BRCA2 complex. (2014)
  • Author(s): Al Abo M, Dejsuphong D, Hirota K, Yonetani Y, Yamazoe M, Kurumizaka H, Takeda S
  • Journal Title: Cancer Res. Volume: 74 Issue: 3 Pages: 797-807
  • DOI: 10.1158/0008-5472.can-13-1443
  • Peer Reviewed
• [Journal Article] Functional analyses of the C terminal half of the Saccharomyces cerevisiae Rad52 protein (2014)
  • Author(s): Kagawa, W., Arai, N., Ichikawa, Y., Saito, K., Sugiyama, S., Saotome, M., Shibata, T. and Kurumizaka, H.
  • Journal Title: Nucleic Acids Res Volume: 42 Issue: 2 Pages: 941-951
  • DOI: 10.1093/nar/gkt986
  • Peer Reviewed
• [Journal Article] Telomeric repeats act as nucleosome-disfavouring sequences in vivo. (2013)
  • Author(s): Ichikawa Y., Morohashi N., Nishimura Y., Kurumizaka H., Shimizu M.
  • Journal Title: Nucleic Acids Res. Volume: 29 Issue: 3 Pages: 1-12
  • DOI: 10.1093/nar/gkt1006
  • Peer Reviewed
• [Journal Article] Sufficient amounts of functional HOP2/MND1 complex promote interhomolog DNA repair but are dispensable for intersister DNA repair during meiosis in Arabidopsis. (2013)
  • Author(s): Uanschou C, Ronceret A., Von Harder M, De Muyt A, Vezon D, Pereira L, Chelysheva L, Kobayashi W, Kurumizaka H, Schlögelhofer P, Grelon M.
  • Journal Title: Plant Cell Volume: 12 Issue: 12 Pages: 4924-4940
  • DOI: 10.1105/tpc.113.118521
  • Peer Reviewed
• [Journal Article] Activation of the SUMO modification system is required for the accumulation of RAD51 at sites containing DNA damage (2013)
  • Author(s): Shima H, Suzuki H, Sun J, Kono K, Shi L, Kinomura A, Horikoshi Y, Ikura T, Ikura M, Kanaar R, Igarashi K, Saitoh H, Kurumizaka H, Tashiro S
  • Journal Title: J Cell Sci Volume: 126 Pages: 5284-5292
  • DOI: 10.1242/jcs.133744
  • Peer Reviewed
• [Journal Article] Homologous pairing activities of two rice RAD51 proteins, RAD51A1 and RAD51A2 (2013)
  • Author(s): Morozumi, Y., Ino, R., Ikawa, S., Mimida, N., Shimizu, T., Toki, S., Ichikawa, H., Shibata, T. and Kurumizaka, H.
  • Journal Title: PLoS ONE Volume: 8 Issue: 10 Pages: e75451-e75451
  • DOI: 10.1371/journal.pone.0075451
  • Peer Reviewed
• [Presentation] Structural studies for epigenetic regulation of genomic DNA (2016)
  • Author(s): Hitoshi Kurumizaka
  • Organizer: 10th International 3R (Replication, Recombination and Repair) Symposium
  • Place of Presentation: 松江
  • Year and Date: 2016-11-13
  • Int'l Joint Research / Invited
• [Presentation] がん細胞で見られるヒストン変異のクロマチン構造・動態変動への影響 (2016)
  • Author(s): 胡桃坂 仁志
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台
  • Year and Date: 2016-09-25
  • Invited
• [Presentation] ALTERED STRUCTURES AND PHYSICAL CHARACTERISTICS OF NUCLEOSOMES CONTAINING CANCER-ASSOCIATED HISTONE MUTATIONS (2016)
  • Author(s): Hitoshi Kurumizaka
  • Organizer: DNA metabolism, genome stability and diseases
  • Place of Presentation: 中国
  • Year and Date: 2016-06-13
  • Int'l Joint Research / Invited
• [Presentation] 天然変性ハブとしてのクロマチン構造 (2016)
  • Author(s): 胡桃坂 仁志
  • Organizer: 横浜NMR研究会
  • Place of Presentation: 理化学研究所、横浜
  • Year and Date: 2016-01-08
  • Invited
• [Presentation] クロマチン構造とダイナミクスの分子機構 (2015)
  • Author(s): 胡桃坂 仁志
  • Organizer: 国際高等研究所 クロマチン・デコーディング
  • Place of Presentation: 国際高等研究所
  • Year and Date: 2015-12-19
  • Invited
• [Presentation] Structural basis of chromatin dynamics (2015)
  • Author(s): Hitoshi Kurumizaka
  • Organizer: 4th International Symposium of the Mathematics on Chromatin Live Dynamic
  • Place of Presentation: JMSアステールプラザ，広島
  • Year and Date: 2015-12-07
  • Int'l Joint Research / Invited
• [Presentation] クロマチン構造によるDNA機能のエピジェネティック制御 (2015)
  • Author(s): 胡桃坂 仁志
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] ヒストンバリアントによるクロマチン動構造制御 (2015)
  • Author(s): 胡桃坂 仁志
  • Organizer: 遺伝研研究会 クロマチン・細胞核構造の形成とダイナミクスによるゲノム機能制御
  • Place of Presentation: 国立遺伝学研究所
  • Year and Date: 2015-10-29
  • Invited
• [Presentation] Structural properties of the nucleosomes containing cancer-associated mutations (2015)
  • Author(s): 胡桃坂 仁志
  • Organizer: 大阪大学蛋白研セミナーNuclear organization and actin-dependent mechanisms in genome stability
  • Place of Presentation: 大阪大学蛋白質研究所
  • Year and Date: 2015-05-18
  • Invited
• [Presentation] Nap1 is required for the homologous recombination reaction in higher-order chromatin containing histone H1" (2014)
  • Author(s): Shinichi Machida, Motoki Takaku, Masae Ikura, Jiying Sun, Hidekazu Suzuki, Wataru Kobayashi, Aiko Kinomura, Akihisa Osakabe, Hiroaki Tachiwana, Yasunori Horikoshi, Atsuhiko Fukuto、 Ryo Matsuda, Kiyoe Ura, Satoshi Tashiro, Tsuyoshi Ikura, Hitoshi Kurumizaka
  • Organizer: The 4D Nucleome 2014
  • Place of Presentation: 広島県廿日市市
  • Year and Date: 2014-12-17 – 2014-12-20
• [Presentation] Nap1は高次クロマチンでの相同組換えを促進する (2014)
  • Author(s): 町田晋一、高久誉大、井倉正枝、孫継英、鈴木秀和、小林航、木野村愛子、越阪部晃永、立和名博昭、堀越保則、福戸敦彦、松田涼、浦聖恵、田代聡、井倉毅、胡桃坂仁志
  • Organizer: 32回染色体ワークショップ、13回核ダイナミックス
  • Place of Presentation: 広島県廿日市市
  • Year and Date: 2014-12-15 – 2014-12-17
• [Presentation] ファンコニ貧血経路とそのキータンパク質FANCD2の機能解析 (2014)
  • Author(s): 高田 穣、勝木 陽子、佐藤 浩一、石合 正道、胡桃坂 仁志
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 日本人ファンコニ貧血患者における新規原因遺伝子UBE2Tの同定 (2014)
  • Author(s): 平 明日香、吉田 健一、佐藤 浩一、嶋本 顕、田原 栄俊、胡桃坂 仁志、小川 誠司、高田 穣、矢部 普正、矢部 みはる
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] DNA鎖間架橋修復で働くFAN1ヌクレアーゼの生化学的機能解析 (2014)
  • Author(s): 平山 恵美子、高橋 大介、佐藤 浩一、高田 穣、胡桃坂 仁志
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Fanconi貧血患者にみられるFANCD2変異体の機能解析 (2014)
  • Author(s): 佐藤 浩一、石合 正道、高田 穣、胡桃坂 仁志
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] ファンコニ経路に働くFANCD2の相同組換えにおける生化学的機能解析 (2014)
  • Author(s): 下向 真代、佐藤 浩一、胡桃坂 仁志
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] In vivoでポジショニングしたヌクレオソームの化学切断法による解析 (2014)
  • Author(s): 布施 智博、市川 雄一、勝俣 光司、向 由起夫、胡桃坂 仁志、清水 光弘
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] マイクロサテライト配列におけるヌクレオソーム形成のin vivoでの評価 (2014)
  • Author(s): 勝俣 光司、井手上 翔、蝦名 瑠衣、渡邊 耀吉、布施 智博、市川 雄一、胡桃坂 仁志、清水 光弘
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] テロメアでのヌクレオソーム形成におけるTRF1およびTRF2の役割 (2014)
  • Author(s): 市川 雄一、宇田 有希、文屋 廣隆、西村 善文、胡桃坂 仁志、清水 光弘
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] RNAi因子を導入した出芽酵母におけるクロマチン構造の解析 (2014)
  • Author(s): 諸星 皓哉、藤井 郁弥、市川 雄一、胡桃坂 仁志、清水 光弘
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Biochemical studies for homologous recombination reaction in chromatin (2014)
  • Author(s): Kurumizaka, H.
  • Organizer: The 9th 3R Symposium
  • Place of Presentation: 御殿場高原ホテル時之梄
  • Year and Date: 2014-11-17 – 2014-11-21
• [Presentation] Biochemical analysis of DMC1-medited homologous pairing in chromatin (2014)
  • Author(s): Wataru Kobayashi, Motoki Takaku, Shinichi Machida, Hiroaki Tachiwana, Yasuyuki Ohkawa, Hitoshi Kurumizaka
  • Organizer: The 9th 3R Symposium
  • Place of Presentation: Goten Kogen Hotel Tokinosumika, Japan
  • Year and Date: 2014-11-17 – 2014-11-21
• [Presentation] The function of linker histone variant H1T in homologous recombination (2014)
  • Author(s): Shinichi Machida, Ryota Hayashida, Atsuhiko Fukuto, Yasunori Horikoshi, Satoshi Tashiro and Hitoshi Kurumizaka
  • Organizer: The 9th 3R Symposium
  • Place of Presentation: Goten Kogen Hotel Tokinosumika, Japan
  • Year and Date: 2014-11-17 – 2014-11-21
• [Presentation] リンカーヒストンバリアントH1Tの相同組換えにおける機能 (2014)
  • Author(s): 町田晋一、林田亮太、加藤大貴、越阪部晃永、小林航、胡桃坂仁志
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] クロマチンにおける減数分裂期特異的相同組換え酵素DMC1の機能解析 (2014)
  • Author(s): 小林 航, 高久 誉大, 町田 晋一, 立和名 博昭, 大川 恭行, 胡桃坂 仁志
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] DNA鎖間架橋除去におけるFAN1ヌクレアーゼの役割 (2014)
  • Author(s): 高橋 大介, 佐藤 浩一, 平山 恵美子, 高田 穣, 胡桃坂 仁志
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] FUNCTIONAL ANALYSES OF RAD51 AND DMC1 RECOMBINASES IN RICE (2014)
  • Author(s): Wataru Kobayashi, Shinichi Machida, and Hitoshi Kurumizaka
  • Organizer: Plant Genome Stability and Change 2014
  • Place of Presentation: Pacific Grove, California, USA
  • Year and Date: 2014-07-17 – 2014-07-21
• [Presentation] BIOCHEMICAL ANALYSES OF RICE DNA RECOMBINASES RAD51 AND DMC1 (2014)
  • Author(s): Kurumizaka, H.
  • Organizer: Plant Genome Stability and Change 2014
  • Place of Presentation: アシロマカンファレンスセンター アメリカ
  • Year and Date: 2014-07-17 – 2014-07-19
• [Presentation] ファンコニ貧血タンパク質に相互作用するFAN1ヌクレアーゼの機能解析 (2014)
  • Author(s): 佐藤 浩一
  • Organizer: 第2回若手の会 「クロマチン動構造 若手交流ワークショップ」
  • Place of Presentation: 北海道 十勝
  • Year and Date: 2014-07-02 – 2014-07-03
• [Presentation] 化学切断法によるin vivoでのヌクレオソームポジショニングの解析 (2014)
  • Author(s): 布施智博、三輪大貴、林優汰、諸橋伸行、市川雄一、胡桃坂仁志、渋沢庸一、清水光弘
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
• [Presentation] 転写調節とクロマチン構造制御に関与する天然変性タンパク質 (2014)
  • Author(s): 清水光弘
  • Organizer: 新学術領域研究「天然変性タンパク質の分子認識機構と機能発現」第3回公開シンポジウム
  • Place of Presentation: 福岡
  • Invited
• [Presentation] Nucleosome-disfavoring properties of telomeric repeat sequences in vivo (2013)
  • Author(s): Yuichi Ichikawa, Nobuyuki Morohashi, Yoshifumi Nishimura, Hitoshi Kurumizaka, Mitsuhiro Shimizu
  • Organizer: The 2013 ASCB Annual Meeting
  • Place of Presentation: New Orleans、LA、USA
• [Presentation] ATTCT repeat sequences can promote nucleosome positioning to repress an inducible gene expression in Saccharomayces cerevisiae (2013)
  • Author(s): Nobuyuki Morohashi, Genta Kobayashi, Yoichi Shibusawa, Tohru Matsuura, Mitsuhiro Shimizu
  • Organizer: The 2013 ASCB Annual Meeting
  • Place of Presentation: New Orleans、LA、USA
• [Presentation] 出芽酵母Ume6における転写活性化および抑制に関与する天然変性領域の同定 (2013)
  • Author(s): 諸橋伸行、大島えりか、市川雄一、大島綾子、加藤尚貴、福地佐斗志、西村善文、胡桃坂仁志、Aaron P. Mitchell、清水光弘
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] テロメア反復配列によるヌクレオソーム形成阻害には，連続したグアニン残基が必須である (2013)
  • Author(s): 市川雄一、諸橋伸行、三界博忠、西村善文、胡桃坂仁志、清水光弘
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] CTGとATTCTリピート配列によるヌクレオソームポジショニング (2013)
  • Author(s): 布施智博、諸橋伸行、市川雄一、林優汰、三輪大貴、松本静香、香川亘、岩崎わかな、立和名博昭、胡桃坂仁志、松浦徹、清水光弘
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] リンカーヒストンを含む高次クロマチン構造における相同組換え機構 (2013)
  • Author(s): 町田 晋一, 高久 誉大, 井倉 正枝, 孫 継英, 鈴木 秀和, 小林 航, 木野村 愛子, 越阪部 晃永, 立和名 博昭, 浦 聖恵, 田代 聡, 井倉 毅, 胡桃坂 仁志
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] In vivoにおけるヌクレオソーム形成に及ぼすテロメア反復配列とその結合因子TRF1の影響 (2013)
  • Author(s): 市川雄一、諸橋伸行、三界博忠、加藤尚貴、西村善文、胡桃坂仁志、清水光弘
  • Organizer: 第31回染色体ワークショップ・第12回核ダイナミクス研究会
  • Place of Presentation: 箱根
• [Presentation] クロマチンでの相同組換え反応におけるDMC1の機能 (2013)
  • Author(s): 小林航、高久誉大、町田晋一、松本亮平、立和名博昭、胡桃坂仁志
  • Organizer: 第22回DNA複製・組換え・修復ワークショップ
  • Place of Presentation: 仙台
• [Presentation] 高次クロマチンにおける相同組換え反応機構 (2013)
  • Author(s): 町田晋一、高久誉大、井倉正枝、孫継英、鈴木秀和、小林航、木野村愛子、越阪部晃永、立和名博昭、浦聖恵、田代聡、井倉毅、胡桃坂仁志
  • Organizer: 第22回DNA複製・組換え・修復ワークショップ
  • Place of Presentation: 仙台
• [Presentation] DNAリピート配列におけるヌクレオソームの形成とポジショニング (2013)
  • Author(s): 清水光弘
  • Organizer: 平成25年度国立遺伝学研究所研究会「クロマチンによる遺伝情報のエピジェネティック制御機構」
  • Place of Presentation: 静岡
  • Invited
• [Presentation] Homologous recombination reaction in higher ordered chromatin (2013)
  • Author(s): Kurumizaka, H.
  • Organizer: The 3rd International conference New Frontier of the Research on RecA-family recombinases and their accessory proteins
  • Place of Presentation: Nantes, France
  • Invited
• [Presentation] シナプトネマ複合体構成因子ヒトSYCP3の生化学的機能解析 (2013)
  • Author(s): 寺本 睦美, 両角 佑一, 小林 航, 高久 誉大, 細谷 紀子, 宮川 清, 胡桃坂 仁志
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] テロメアリピート配列はin vivoでのヌクレオソーム形成を阻害する (2013)
  • Author(s): 市川雄一、諸橋伸行、三界博忠、西村善文、胡桃坂仁志、清水光弘
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] 減数分裂期特異的な相同組換え酵素DMC1のクロマチンにおける反応機構 (2013)
  • Author(s): 小林航、高久誉大、町田晋一、両角祐一、松本亮平、立和名博昭、胡桃坂仁志
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] 相同組換えにおける高次クロマチンの動的な制御 (2013)
  • Author(s): 町田 晋一, 高久 誉大, 井倉 正枝, 孫 継英, 鈴木 秀和, 小林 航, 越阪部 晃永, 立和名 博昭, 浦 聖恵, 田代 聡, 井倉 毅, 胡桃坂 仁志
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] Nu G-clusters play crucial roles in the nucleosome-disfavoring properties of telomeric repeat sequences in vivo (2013)
  • Author(s): Yuichi Ichikawa, Nobuyuki Morohashi, Yoshifumi Nishimura, Hitoshi Kurumizaka, Mitsuhiro Shimizu
  • Organizer: Message from yeast to Epigenetics～Yeast clarifies the frontiers of life science～
  • Place of Presentation: 福井
• [Book] がん基盤生物学（担当：相同組換え関連因子を標的にしたがん治療薬研究） (2013)
  • Author(s): 高久誉大,町田晋一,胡桃坂仁志
  • Total Pages: 6
  • Publisher: 南山堂
• [Remarks] 胡桃坂研究室ホームページ
  • URL: http://www.eb.waseda.ac.jp/kurumizaka/
• [Remarks] 清水研究室ホームページ
  • URL: http://www.hino.meisei-u.ac.jp/chem/LBT/Shimizu01.html
• [Remarks] 胡桃坂研究室ホームページ
  • URL: http://www.eb.waseda.ac.jp/kurumizaka/
• [Remarks] 清水研究室ホームページ
  • URL: http://www.hino.meisei-u.ac.jp/chem/LBT/Shimizu01.html