Comparative genome analysis of parthenogenetic nematode
| Project/Area Number |
25250025
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
KOHARA Yuji 国立遺伝学研究所, 系統生物研究センター, 特任教授 (70135292)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥45,370,000 (Direct Cost: ¥34,900,000、Indirect Cost: ¥10,470,000)
Fiscal Year 2016: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2015: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2014: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2013: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
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| Keywords | ゲノムアセンブリ / 線虫 / 単為生殖 / 進化 / 減数分裂 / ゲノム多様性 / 単為発生 / 染色体 / 遺伝子 / 発生・分化 |
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| Outline of Final Research Achievements |
Although sexual reproduction is prevailing among eukaryotes, the nematode Diploscapter coronatus, a close relative of the model C. elegans, reproduces parthenogenetically. Neither males nor sperm have been observed and some steps of meiosis are apparently skipped in this species. To uncover the genomic changes associated with parthenogenetic reproduction, we carried out a genome analysis and we found that nearly 90% of the 170 Mbp draft genome sequences constitute homologous pairs with a 5.7% heterozygosity on average, meaning that the 170 Mbp sequences correspond to the diploid genome. Fluorescent staining showed that the D. coronatus genome consists of two chromosomes (2n = 2). Although 59 % of the C. elegans genes were found in the genome, many genes including those involved in sex determination and meiosis were missing or very divergent. Among them the defect of meiotic kleisins (rec-8 and coh-3/4) may be related to parthenogenetic reproduction.
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Report
(5 results)
Research Products
(14 results)
