| Budget Amount *help |
¥45,370,000 (Direct Cost: ¥34,900,000、Indirect Cost: ¥10,470,000)
Fiscal Year 2016: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2015: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2014: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2013: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
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| Outline of Final Research Achievements |
Although sexual reproduction is prevailing among eukaryotes, the nematode Diploscapter coronatus, a close relative of the model C. elegans, reproduces parthenogenetically. Neither males nor sperm have been observed and some steps of meiosis are apparently skipped in this species. To uncover the genomic changes associated with parthenogenetic reproduction, we carried out a genome analysis and we found that nearly 90% of the 170 Mbp draft genome sequences constitute homologous pairs with a 5.7% heterozygosity on average, meaning that the 170 Mbp sequences correspond to the diploid genome. Fluorescent staining showed that the D. coronatus genome consists of two chromosomes (2n = 2). Although 59 % of the C. elegans genes were found in the genome, many genes including those involved in sex determination and meiosis were missing or very divergent. Among them the defect of meiotic kleisins (rec-8 and coh-3/4) may be related to parthenogenetic reproduction.
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