| Budget Amount *help |
¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
Fiscal Year 2015: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2014: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2013: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
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| Outline of Final Research Achievements |
Double-stranded DNA must be unwound to single-stranded DNA to initiate DNA replication. Mcm2-7, the core of the helicase that unwinds DNA, is loaded to replication origins first and then Cdc45 and GINS associate with the loaded Mcm2-7 to form an active helicase. We reveal that the Sld3-Sld7 complex, which is essential for this activation, comprises two Sld3 molecules connected by two Sld7 molecules and suggest that this structure is important for the initiation of DNA replication. Moreover, we confirm that DNA polymerase epsilon also works for this activation step. We further devise the way to analyze the relationship between chromatin, cell cycle checkpoints and DNA replication.
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