Molecular mechanism of the initiation in eukaryotic chromosomal DNA replication
Project/Area Number |
25251005
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Molecular biology
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Research Institution | National Institute of Genetics |
Principal Investigator |
ARAKI Hiroyuki 国立遺伝学研究所, 細胞遺伝研究系, 教授 (20151160)
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Co-Investigator(Kenkyū-buntansha) |
HIZUME Kohji 国立遺伝学研究所, 細胞遺伝研究系, 助教 (10378846)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
Fiscal Year 2015: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2014: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2013: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
|
Keywords | DNA複製 / 細胞周期 / クロマチン / 酵母 / 染色体 / 複製開始 |
Outline of Final Research Achievements |
Double-stranded DNA must be unwound to single-stranded DNA to initiate DNA replication. Mcm2-7, the core of the helicase that unwinds DNA, is loaded to replication origins first and then Cdc45 and GINS associate with the loaded Mcm2-7 to form an active helicase. We reveal that the Sld3-Sld7 complex, which is essential for this activation, comprises two Sld3 molecules connected by two Sld7 molecules and suggest that this structure is important for the initiation of DNA replication. Moreover, we confirm that DNA polymerase epsilon also works for this activation step. We further devise the way to analyze the relationship between chromatin, cell cycle checkpoints and DNA replication.
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Report
(4 results)
Research Products
(37 results)
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[Journal Article] Fidelity consequences of the impaired interaction between DNA polymerase epsilon and the GINS complex2015
Author(s)
Garbacz, M., Araki, H., Flis, K., Bebenek, A., Zawada, A., Jonczyk, P., Makiela-Dzbenska, K. and Fijiakowska, I. J.
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Journal Title
DNA repair
Volume: 29
Pages: 23-35
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Bidirectional initiation of chromosomal DNA replication in budding yeast2015
Author(s)
Araki, H., Itou, H., Makino, N., Yagura, M., Endo, S. and Muramatsu, S.
Organizer
2015 Cold Spring Harbor Meeting on "Eukaryotic DNA replication & genome maintenance"
Place of Presentation
Cold Spring Harbor, NY, USA
Year and Date
2015-09-02
Related Report
Int'l Joint Research
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[Presentation] Dpb11/TopBP1-GINS interaction is required for efficient initiation of DNA replication2013
Author(s)
Tanaka, S., Komeda,Y., Umemori,T., Kubota,Y., Takisawa,H., and Araki,H.
Organizer
2013 Cold Spring Harbor Laboratory Meeting on EUKARYOTIC DNA REPLICATION & GENOME MAINTENANCE
Place of Presentation
Cold Spring Harbor, NY(USA)
Related Report
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