| Budget Amount *help |
¥46,150,000 (Direct Cost: ¥35,500,000、Indirect Cost: ¥10,650,000)
Fiscal Year 2015: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2013: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
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| Outline of Final Research Achievements |
DOCK family proteins are atypical guanine nucleotide exchange factors that mediate GTP-GDP exchange reaction through their DHR-2 domain. Recent evidence indicates that DOCK family proteins are involved in many cellular functions, yet their regulatory mechanisms are poorly understood. In this study, we have analyzed signaling and functions of DOCK family proteins and obtained the following important findings: 1) DOCK5 acts downstream of FCεRI receptor and critically regulates mast cell degranulation. 2) DOCK1 plays a key role in invasion and metastasis of breast cancer cells. 3) The phosphorylation sites of DOCK2 in response to chemokine stimulation were identified. 4) The critical domain required for membrane localization of DOCK8 was identified. 5) The previously unknown function of DOCK8 and its regulatory mechanism were discovered.
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