Elucidation of the crosstalk between environmental stress responses and regulation of plant growth

• Project/Area Number: 25251031
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The University of Tokyo
• Principal Investigator: Yamaguchi-Shinozaki Kazuko 東京大学, 農学生命科学研究科, 教授 (30221295)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥47,320,000 (Direct Cost: ¥36,400,000、Indirect Cost: ¥10,920,000); Fiscal Year 2015: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000); Fiscal Year 2014: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000); Fiscal Year 2013: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
• Keywords: 環境応答 / 植物 / 生長制御 / 細胞伸長 / 光合成 / ストレス / 発現制御 / 転写因子 / 翻訳後調節

Outline of Final Research Achievements

To elucidate the transcriptional network to suppress the plant growth under drought stress conditions, we analyzed transcriptional regulation of two genes for transcription factors (PIF4, GLK) that function on the crosstalk between stress responses and regulation of plant growth. Down-regulation of the PIF4 gene was confirmed and its promoter region was analyzed to identify cis-acting elements that regulate its expression under both circadian and drought conditions. The DNA-binding protein interacting with the identified cis-acting element was isolated and designated PBP1. PBP1 functioned as a transcriptional activator for the expression of PIF4. We also analyzed promoter regions of photosynthesis-related genes and identified GLK binding sites as positive cis-acting elements. The expression of GLK was reduced under drought condition indicating that reduction of the GLK expression may be important for the reduced expression of photosynthesis-related genes under drought conditions.

Research Products

• [Journal Article] The Arabidopsis transcriptional regulator DPB3-1 enhances heat stress tolerance without growth retardation in rice. (2016)
  • Author(s): Sato H, Todaka D, Kudo M, Mizoi J, Kidokoro S, Zhao Y, Shinozaki K, and Yamaguchi-Shinozaki K
  • Journal Title: Plant Biotechnol. J. Volume: Feb Issue: 8 Pages: 1-12
  • DOI: 10.1111/pbi.12535
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The transcriptional cascade in the heat stress response of Arabidopsis is strictly regulated at the expression levels of transcription factors. (2016)
  • Author(s): Ohama N, Kusakabe K, Mizoi J, Zhao H, Kidokoro S, Koizumi S, Takahashi F, Ishida T, Yanagisawa S, Shinozaki K, and Yamaguchi-Shinozaki K.
  • Journal Title: Plant Cell Volume: 28(1) Issue: 1 Pages: 181-201
  • DOI: 10.1105/tpc.15.00435
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] SNAC-As, stress-responsive NAC transcription factors, mediate ABA-inducible leaf senescence. (2015)
  • Author(s): Takasaki H, Maruyama K, Takahashi F, Fujita M, Yoshida T, Nakashima K, Myouga F, Toyooka K, Yamaguchi-Shinozaki K, Shinozaki K.
  • Journal Title: Plant Journal Volume: 84 Issue: 6 Pages: 1114-1123
  • DOI: 10.1111/tpj.13067
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 植物の乾燥・高温応答の解明と再生作物の開発に向けて (2015)
  • Author(s): 佐藤輝、篠崎和子
  • Journal Title: Bio Industry Volume: 32(12) Pages: 25-31
• [Journal Article] Omics Approaches Toward Defining the Comprehensive Abscisic Acid Signaling Network in Plants. (2015)
  • Author(s): Yoshida T, Mogami J, and Yamaguchi-Shinozaki K
  • Journal Title: Plant Cell Physiol. Volume: 56(6) Issue: 6 Pages: 1043-1052
  • DOI: 10.1093/pcp/pcv060
  • Peer Reviewed / Open Access
• [Journal Article] 高温環境を生き抜くための植物の転写制御機構 (2015)
  • Author(s): 大濱直彦、篠崎和子
  • Journal Title: 化学と生物 Volume: 53(10) Pages: 696-702
  • NAID: 130005416108
• [Journal Article] Two Distinct Families of Protein Kinases Are Required for Plant Growth under High External Mg2+ Concentrations in Arabidopsis (2015)
  • Author(s): Junro Mogami, Yasunari Fujita, Takuya Yoshida, Yoshifumi Tsukiori, Hirofumi Nakagami, Yuko Nomura, Toru Fujiwara, Sho Nishida, Shuichi Yanagisawa, Tetsuya Ishida, Fuminori Takahashi, Kyoko Morimoto, Satoshi Kidokoro, Junya Mizoi, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki
  • Journal Title: Plant Physiology Volume: 167 Issue: 3 Pages: 1039-1057
  • DOI: 10.1104/pp.114.249870
  • Peer Reviewed / Open Access
• [Journal Article] Soybean DREB1/CBF-type transcription factors function in heat and drought as well as cold stress-responsive gene expression (2015)
  • Author(s): Satoshi Kidokoro, Keitaro Watanabe, Teppei Ohori, Takashi Moriwaki, Kyonoshin Maruyama, Junya Mizoi, Nang Myint Phyu Sin Htwe, Yasunari Fujita, Sachiko Sekita, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki
  • Journal Title: Plant J. Volume: 81 Issue: 3 Pages: 505-518
  • DOI: 10.1111/tpj.12746
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Four Arabidopsis AREB/ABF transcription factors function predominantly in gene expression downstream of SnRK2 kinases in abscisic-acid signaling in response to osmotic stress (2015)
  • Author(s): Takuya Yoshida, Yasunari Fujita, Kyonoshin Maruyama, Junro Mogami, Daisuke Todaka, Kazuo Shinozaki & Kazuko Yamaguchi-Shinozaki
  • Journal Title: Plant Cell and Enviorment Volume: 38 Issue: 1 Pages: 35-49
  • DOI: 10.1111/pce.12351
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Recent advances in the dissection of drought-stress regulatory networks and strategies for development of drought-tolerant transgenic rice plants (2015)
  • Author(s): Daisuke Todaka, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki
  • Journal Title: Front Plant Sci Volume: 6 Pages: 1-20
  • DOI: 10.3389/fpls.2015.00084
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Arabidopsis DPB3-1, a DREB2A Interactor, Specifically Enhances Heat Stress-Induced Gene Expression by Forming a Heat Stress-Specific Transcriptional Complex with NF-Y Subunits (2014)
  • Author(s): Hikaru Sato, Junya Mizoi, Hidenori Tanaka, Kyonosin Maruyama, Feng Qin, Yuriko Osakabe, Kyoko Morimoto, Teppei Ohori, Kazuya Kusakabe, Maika Nagata, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki
  • Journal Title: Plant Cell Volume: 26 Issue: 12 Pages: 4954-4973
  • DOI: 10.1105/tpc.114.132928
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Functional analysis of the Hikeshi-like protein that interacts with HSP70 in Arabidopsis (2014)
  • Author(s): Shinya Koizumi, Naohiko Ohama, Junya Mizoi, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 450 Issue: 1 Pages: 396-400
  • DOI: 10.1016/j.bbrc.2014.05.128
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Integrated analysis of the effects of cold and dehydration on rice metabolites, phytohormones, and transcripts (2014)
  • Author(s): Maruyama K, Urano K, Yoshiwara K, Morishita Y, Sakurai N, Suzuki H, Kojima M, Sakakibara H, Shibata D, Saito K, Shinozaki K, Yamaguchi-Shinozaki K
  • Journal Title: Plant Physiol. Volume: 164(4) Issue: 4 Pages: 1759-1771
  • DOI: 10.1104/pp.113.231720
  • Peer Reviewed / Open Access
• [Journal Article] The transcriptional regulatory network in the drought response and its crosstalk in abiotic stress responses including drought, cold, and heat (2014)
  • Author(s): Nakashima K, Yamaguchi-Shinozaki K, Shinozaki K
  • Journal Title: Front Plant Sci. Volume: 16; 5:170 Pages: 1-7
  • DOI: 10.3389/fpls.2014.00170
  • Peer Reviewed / Open Access
• [Journal Article] ABA-dependent and ABA-independent signaling in response to osmotic stress in plants (2014)
  • Author(s): Takuya Yoshida, Junro Mogami and Kazuko Yamaguchi-Shinozaki
  • Journal Title: Current Opinion in Plant Biology Volume: 21 Pages: 133-139
  • DOI: 10.1016/j.pbi.2014.07.009
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 環境ストレス時の生長制御機構の解析 (2016)
  • Author(s): 戸高 大輔、趙 宇、吉田 拓也、工藤 まどか、Alisdair R. Fernie、篠崎 一雄、篠崎 和子
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡、岩手
  • Year and Date: 2016-03-18
• [Presentation] 高温ストレス下における植物の生長制御機構の解明 (2016)
  • Author(s): 小泉 慎也、城所 聡、大濱 直彦、中嶋 正敏、篠崎 一雄、篠崎 和子
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡、岩手
  • Year and Date: 2016-03-18
• [Presentation] 遺伝子集積法による乾燥ストレス耐性植物の生長促進制御 (2016)
  • Author(s): 工藤 まどか、城所 聡、溝井 順哉、戸高 大輔、篠崎 和子
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡、岩手
  • Year and Date: 2016-03-18
• [Presentation] 環境ストレス応答の分子機構の解明と耐性作物開発 (2015)
  • Author(s): 篠崎和子
  • Organizer: 東京理科大学 アグリ・バイオ工学研究部門公開シンポジウム
  • Place of Presentation: 葛飾、東京
  • Year and Date: 2015-07-17
  • Invited
• [Presentation] Regulatory networks for osmotic and heat stress responses in plant, (2015)
  • Author(s): Yamaguchi-Shinozaki K
  • Organizer: the International Symposium on “Plant Responses to Stress”
  • Place of Presentation: Beijing, China
  • Year and Date: 2015-06-15
  • Int'l Joint Research / Invited
• [Presentation] 環境ストレス時の生長制御機構の解析 (2015)
  • Author(s): 戸高大輔，趙宇，工藤まどか，篠崎一雄，篠崎和子
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16
• [Presentation] バイオマス生産性を向上させた乾燥ストレス耐性植物の創出 (2015)
  • Author(s): 工藤まどか、戸高大輔、篠崎和子
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16
• [Presentation] Analysis of transcriptional regulation of Arabidopsis PIF family genes in response to abiotic stresses (2015)
  • Author(s): Moon J-S, Kidokoro S, Todaka D, Igusa S, Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16
• [Presentation] Water and Plants (2014)
  • Author(s): Yamaguchi-Shinozaki K
  • Organizer: Women in Global Science 2014
  • Place of Presentation: Hokkaido, Japan
  • Year and Date: 2014-10-27
  • Int'l Joint Research / Invited
• [Presentation] 植物の乾燥ストレス応答と成長制御 (2014)
  • Author(s): 篠崎和子
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 川崎、神奈川
  • Year and Date: 2014-09-12
  • Invited
• [Presentation] 植物の環境ストレス耐性機構と分子育種 (2014)
  • Author(s): 篠崎和子
  • Organizer: 第53回ガンマーフィールドシンポジウム
  • Place of Presentation: 水戸、茨城
  • Year and Date: 2014-07-16
  • Invited
• [Presentation] 環境ストレス時の細胞伸長と生長制御 (2014)
  • Author(s): 戸高大輔，工藤まどか，趙宇，有賀遥平，本多剛志，佐藤輝，篠崎一雄，篠崎和子
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
• [Presentation] シロイヌナズナにおける低温誘導性の転写因子遺伝子DREB1の転写制御機構の解析 (2014)
  • Author(s): 戸田 智美，城所 聡，溝井 順哉，篠崎 一雄，篠崎 和子
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
• [Presentation] ストレス条件下における光合成関連遺伝子群の発現抑制機構に関する解析 (2014)
  • Author(s): 本多 剛志，城所 聡，戸高 大輔，篠崎 一雄，篠崎 和子
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
• [Presentation] Plant responses and adaptation to water stress (2013)
  • Author(s): Kazuko Yamaguchi-Shinozaki
  • Organizer: 7th EPSO Conference
  • Place of Presentation: Porto Heri, Greece
  • Invited