| Budget Amount *help |
¥47,320,000 (Direct Cost: ¥36,400,000、Indirect Cost: ¥10,920,000)
Fiscal Year 2015: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2014: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2013: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
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| Outline of Final Research Achievements |
To elucidate the transcriptional network to suppress the plant growth under drought stress conditions, we analyzed transcriptional regulation of two genes for transcription factors (PIF4, GLK) that function on the crosstalk between stress responses and regulation of plant growth. Down-regulation of the PIF4 gene was confirmed and its promoter region was analyzed to identify cis-acting elements that regulate its expression under both circadian and drought conditions. The DNA-binding protein interacting with the identified cis-acting element was isolated and designated PBP1. PBP1 functioned as a transcriptional activator for the expression of PIF4. We also analyzed promoter regions of photosynthesis-related genes and identified GLK binding sites as positive cis-acting elements. The expression of GLK was reduced under drought condition indicating that reduction of the GLK expression may be important for the reduced expression of photosynthesis-related genes under drought conditions.
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