Regulation of chloroplast function by the NDH complex

• Project/Area Number: 25251032
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: SHIKANAI TOSHIHARU 京都大学, 理学(系)研究科(研究院), 教授 (70273852)
• Project Period (FY): 2013-10-21 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥44,460,000 (Direct Cost: ¥34,200,000、Indirect Cost: ¥10,260,000); Fiscal Year 2015: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000); Fiscal Year 2014: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000); Fiscal Year 2013: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
• Keywords: 葉緑体 / 光合成 / チラコイド膜 / NDH / 光化学系Ⅰ / フェレドキシン / アセンブリ / 超複合体 / LhcI / 電子伝達 / 光化学系 I / 分子進化 / シロイヌナズナ / ゼニゴケ

Outline of Final Research Achievements

Chloroplast NDH accepts electrons from ferredoxin rather than NAD(P)H. The SH3 domain of the NdhS protein has positive charge, contributing to the electrostatic interaction with ferredoxin with negative charge. In Arabidopsis, chloroplast NDH mediates PSI cyclic electron transport, contributing to the formation of proton motive force across the thylakoid membrane. Chloroplast NDH forms the supercomplex with PSI, in the process which Lhca5 and Lhca6 function as linkers. Lhca6 originated from Lhca2 during the evolution of land plants. Analysis of chimeric proteins between two LhcI proteins clarified the Lhca6 region required for the linker function. We also obtained the molecular information on CRR3 and CRR9, which were required for the assembly of chloroplast NDH.

Research Products

• [Journal Article] Regulatory network of proton motive force: Contribution of cyclic electron transport around photosystem I (2016)
  • Author(s): Shikanai T.
  • Journal Title: Photosynthesis Res. Volume: 未定 Issue: 3 Pages: 253-260
  • DOI: 10.1007/s11120-016-0227-0
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Artificial remodeling of alternative electron flow by flavodiiron proteins in Arabidopsis (2016)
  • Author(s): Yamamoto H, Takahashi S, Badger RM, Shikanai T
  • Journal Title: Nature Plants Volume: 2 Issue: 3 Pages: 16012-16012
  • DOI: 10.1038/nplants.2016.12
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] NDH-1 and NDH-2 plastoquinone reductases in oxygenic photosynthesis (2016)
  • Author(s): Peltier G., Aro E.-M. and Shikanai T.
  • Journal Title: Annu. Rev. Plant Biol. Volume: 67 Issue: 1 Pages: 55-80
  • DOI: 10.1146/annurev-arplant-043014-114752
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Role of cyclic electron transport around photosystem I in regulating proton motive force (2015)
  • Author(s): Wang C., Yamamoto H. and Shikanai T.
  • Journal Title: Biochim. Biophys. Acta Volume: 1847 Issue: 9 Pages: 931-938
  • DOI: 10.1016/j.bbabio.2014.11.013
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Photosystem I cyclic electron flow via chloroplast NADH dehydrogenase-like complex performs a physiological role for photosynthesis at low light (2015)
  • Author(s): Yamori W., Shikanai T. and Makino A.
  • Journal Title: Sci Rep Volume: 5 Issue: 1 Pages: 13908-13908
  • DOI: 10.1038/srep13908
  • NAID: 120005822772
  • Peer Reviewed
• [Journal Article] A single amino acid alteration in PGR5 confers resistance to antimycin A in cyclic electron transport around PSI (2013)
  • Author(s): Sugimoto K., Okegawa Y., Tohri A., Long T.A., Sarah F.S., Hisabori T. and Shikanai T
  • Journal Title: Plant Cell Physiol. Volume: 54 Issue: 9 Pages: 1525-1534
  • DOI: 10.1093/pcp/pct098
  • Peer Reviewed
• [Journal Article] Mutagenesis of Individual Pentatricopeptide Repeat Motifs Affects RNA Binding Activity and Reveals Functional Partitioning of Arabidopsis PROTONGRADIENT REGULATION3 (2013)
  • Author(s): Fujii S, Sato N, Shikanai T
  • Journal Title: Plant Cell Volume: 25 Issue: 8 Pages: 1-11
  • DOI: 10.1105/tpc.113.112193
  • Peer Reviewed
• [Journal Article] Subfunctionalization of sigma factors during the evolution of land plants based on mutant analysis of liverwort (Marchantia polymorpha L.) MpSIG1. (2013)
  • Author(s): Ueda, M., Takami, T., Peng, L., Ishizaki, K., Kohchi, T., Shikanai, T. and Nishimura, Y.
  • Journal Title: Genome Biology and Evolution Volume: 5 Issue: 10 Pages: 1836-1848
  • DOI: 10.1093/gbe/evt137
  • NAID: 120005373074
  • Peer Reviewed
• [Journal Article] Antimycin A-like molecules inhibit cyclic electron transport around photosystem I in ruptured chloroplasts (2013)
  • Author(s): Taira Y., Okegawa Y., Sugimoto K., Abe M., Miyoshi H. and Shikanai T
  • Journal Title: FEBS Open Bio. Volume: 3 Issue: 1 Pages: 406-410
  • DOI: 10.1016/j.fob.2013.09.007
  • Peer Reviewed / Open Access
• [Journal Article] In planta mutagenesis of Src homology 3 domain-like fold of NdhS, a ferredoxin-binding subunit of the chloroplast NADH dehydrogenase-like complex in Arabidopsis. A conserved Arg-193 plays a critical role in ferredoxin binding (2013)
  • Author(s): Yamamoto H. and Shikanai T
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 51 Pages: 36328-36337
  • DOI: 10.1074/jbc.m113.511584
  • Peer Reviewed
• [Presentation] Regulation of photosynthesis by alternative electron flow (2015)
  • Author(s): Shikanai T.
  • Organizer: China-Germany Binational Conference "Dynamics in Photosynthesis"
  • Place of Presentation: Tutzing, Germany
  • Year and Date: 2015-11-09
  • Int'l Joint Research / Invited
• [Presentation] Regulation of photosynthesis by alternative electron flow (2015)
  • Author(s): Shikanai T.
  • Organizer: International Symposium on Dynamics and Regulation of Photosynthesis
  • Place of Presentation: 奈良
  • Year and Date: 2015-10-29
  • Int'l Joint Research / Invited
• [Presentation] Regulation of proton motive force in chloroplasts (2015)
  • Author(s): Shikanai T.
  • Organizer: International Copnference Photosynthesis Research for Sustainability
  • Place of Presentation: Crete, Greece
  • Year and Date: 2015-09-21
  • Int'l Joint Research / Invited
• [Presentation] Regulation of proton motive force in chloroplasts (2015)
  • Author(s): Shikanai T.
  • Organizer: Gordon Research Conference Photosynthesis
  • Place of Presentation: Waltman, USA
  • Year and Date: 2015-06-27
  • Int'l Joint Research / Invited
• [Presentation] Cell-type specific expression of chloroplast genes in maize (2015)
  • Author(s): Shikanai T.
  • Organizer: Gordon Research Conference
  • Place of Presentation: Ventura, USA
  • Year and Date: 2015-01-18
  • Int'l Joint Research / Invited
• [Presentation] Regulation of photosynthetic electron transport by cyclic electron transport around PSI (2014)
  • Author(s): Shikanai T.
  • Organizer: Japanese-Finish Seminar 2014
  • Place of Presentation: 札幌
  • Year and Date: 2014-10-09
  • Int'l Joint Research / Invited
• [Presentation] Regulation of photosynthesis by PSI cyclic electron transport. (2014)
  • Author(s): Shikanai T.
  • Organizer: 16th International Congress on Photobiology
  • Place of Presentation: Cordoba, Argentina
  • Year and Date: 2014-09-06
  • Int'l Joint Research / Invited
• [Presentation] Machinery and physiological function of PSI cyclic electron transport (2014)
  • Author(s): Shikanai T.
  • Organizer: International Symposium on the Regulation of Photosynthetic Function
  • Place of Presentation: Guilin, China
  • Year and Date: 2014-08-16
  • Int'l Joint Research / Invited
• [Presentation] NDH-光化学系Ⅰ超複合体に介在するLhca6の分子進化 (2014)
  • Author(s): 大谷拓人，山本宏，鹿内利治
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山
  • Year and Date: 2014-03-18 – 2014-03-20
• [Presentation] Machinery and function of PSI cyclic electron transport. (2014)
  • Author(s): Shikanai T
  • Organizer: 2nd Kyoto-Bristol Symposium
  • Place of Presentation: 京都
  • Year and Date: 2014-01-10
  • Invited
• [Presentation] Regulation of photosynthetic electron transport. (2013)
  • Author(s): Shikanai T
  • Organizer: NC-CARP•CREST/さきがけ国際シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2013-11-08
  • Invited
• [Book] Evolution of photosynthetic NDH-1: Structure and physiological function. W.A. Cramer and T. Kallas (eds.), Cytochrome complexes: Evolution, Structures, Energy Transduction, and Signaling, Advances in Photosynthesis and Respiration (2016)
  • Author(s): Shikanai T. and Aro E.-M.
  • Publisher: Springer Science+Business Media Dordrecht
• [Book] CRC Press (2015)
  • Author(s): Ifuku K. and Shikanai T.
  • Total Pages: 19
  • Publisher: Regulation of photosynthetic electron transport via supercomplex formation in thylakoid membrane. R.R. Louro and I. Diaz-Moreno (eds.), Redox proteins in supercomplezes and signalosomes.