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Characterization of mechanisms for coordinated proliferation of the eukaryotic host cell and the chloroplast

Research Project

Project/Area Number 25251039
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Evolutionary biology
Research InstitutionNational Institute of Genetics

Principal Investigator

MIYAGISHIMA Shin-ya  国立遺伝学研究所, 細胞遺伝研究系, 教授 (00443036)

Project Period (FY) 2013-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥37,180,000 (Direct Cost: ¥28,600,000、Indirect Cost: ¥8,580,000)
Fiscal Year 2016: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2015: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2014: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2013: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Keywords細胞内共生 / 葉緑体分裂 / 細胞周期 / 細胞分裂 / 葉緑体 / 光合成 / 葉緑体光合成
Outline of Final Research Achievements

We aimed to understand the mechanism for coordinated proliferation of the eukaryotic host cell and the chloroplast which played a pivotal role in endosymbiotic establishment of the chloroplast. By characterizing eukaryotic algae, we found (1) that the division of chloroplast of both primary and secondary endosymbiotic origin is restricted to S phase by S-phase-specific expression of nucleus-encoded chloroplast division genes/proteins, (2) that initiation of chloroplast division site constriction is required for the host cell cycle progression, and (3) that the host cell cycle progression is restricted to night when photosynthesis does not operate to avoid photosynthetic oxidative stress. These results suggest that the division synchronization of the host cell and the chloroplast is enabled by interactive restrictions between the host cell and the chloroplast.

Report

(5 results)
  • 2017 Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (24 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (12 results) (of which Peer Reviewed: 11 results,  Open Access: 9 results,  Acknowledgement Compliant: 6 results) Presentation (11 results) (of which Int'l Joint Research: 2 results,  Invited: 8 results)

  • [Int'l Joint Research] Institute for Plant Biochemistry(Germany)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Hierarchal order in the formation of chloroplast division machinery in the red alga Cyanidioschyzon merolae2017

    • Author(s)
      Sumiya, N. and Miyagishima, S.
    • Journal Title

      Commun. Integr. Biol.

      Volume: 10 Issue: 2 Pages: e1294298-e1294298

    • DOI

      10.1080/19420889.2017.1294298

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Development of a double nuclear gene-targeting method by two-step transformation based on a newly established chloramphenicol-selection system in the red alga Cyanidioschyzon merolae2017

    • Author(s)
      Fujiwara, T., Ohnuma, M., Kuroiwa, T., Ohbayashi, R., Hirooka, S.and Miyagishima, S.
    • Journal Title

      Front. Plant Sci.

      Volume: 8 Pages: 343-343

    • DOI

      10.3389/fpls.2017.00343

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Chloroplast division: A handshake across membranes2017

    • Author(s)
      Miyagishima, S.
    • Journal Title

      Nature Plants

      Volume: 3 Pages: 17025-17025

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Cultivation of acidophilic algae Galdieria sulphuraria and Pseudochlorella sp. YKT1 in media derived from acidic hot springs2016

    • Author(s)
      Hirooka, S., and Miyagishima, S.
    • Journal Title

      Front. Microbiol.

      Volume: 7

    • DOI

      10.3389/fmicb.2016.02022

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Chloroplast division checkpoint in eukaryotic algae2016

    • Author(s)
      Sumiya, N., Fujiwara, T., Era, A. and Miyagishima, S.
    • Journal Title

      Proc. Natl. Acad. Sci. USA.

      Volume: 113 Issue: 47

    • DOI

      10.1073/pnas.1612872113

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Expression of cyanobacterial acyl-ACP reductase elevates the triacylglycerol level in the red alga Cyanidioschyzon merolae.2015

    • Author(s)
      Nobuko Sumiya, Yasuko Kawase, Junpei Hayakawa, Mami Matsuda, Mami Nakamura, Atsuko Era, Kan Tanaka, Akihiko Kondo, Tomohisa Hasunuma, Sousuke Imamura, Shin-ya Miyagishima
    • Journal Title

      Plant Cell Physiol.

      Volume: 56 Issue: 10 Pages: 1962-1980

    • DOI

      10.1093/pcp/pcv120

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A nitrogen source-dependent inducible and repressible gene expression system in the red alga Cyanidioschyzon merolae.2015

    • Author(s)
      Takayuki Fujiwara, Yu Kanesaki, Shunsuke Hirooka, Atsuko Era, Nobuko Sumiya, Hirofumi Yoshikawa, Kan Tanaka, Shin-ya Miyagishima
    • Journal Title

      Front. Plant Sci.

      Volume: 6 Pages: 657-657

    • DOI

      10.3389/fpls.2015.00657

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Translation-independent circadian control of the cell cycle in a unicellul ar photosynthetic eukaryote.2014

    • Author(s)
      Miyagishima SY, Fujiwara T, Sumiya N, Hirooka S, Nakano A, Kabeya Y, Nakamura M.
    • Journal Title

      Nature Communications

      Volume: 5 Issue: 1 Pages: 3807-3807

    • DOI

      10.1038/ncomms4807

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Acidophilic Green Alga Pseudochlorella sp. YKT1 Accumulates High Amount of Lipid Droplets under a Nitrogen-depleted Condition at a Low-pH2014

    • Author(s)
      Hirooka, S., Higuchi, S., Uzuka, A., Nozaki, H., and Miyagishima, S.
    • Journal Title

      PLOS ONE

      Volume: 9 Issue: 9 Pages: e107702-e107702

    • DOI

      10.1371/journal.pone.0107702

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] FtsZ-less prokaryotic cell division as well as FtsZ- and dynamin-less chloroplast and non-photosynthetic plastid division2014

    • Author(s)
      Miyagishima, S., Nakamura, M., Uzuka, A., and Era, A.
    • Journal Title

      Front. Plant Sci.

      Volume: 5 Pages: 459-459

    • DOI

      10.3389/fpls.2014.00459

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Development of a heat-shock inducible gene expression system in the red alga Cyanidioschyzon merolae.2014

    • Author(s)
      Sumiya N, Fujiwara T, Kobayashi Y, Misumi O, Miyagishima SY.
    • Journal Title

      PLoS One.

      Volume: 9 Issue: 10 Pages: e111261-e111261

    • DOI

      10.1371/journal.pone.0111261

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] DipM is required for peptidoglycan hydrolysis during chloroplast division.2014

    • Author(s)
      Miyagishima SY, Kabeya Y, Sugita C, Sugita M, Fujiwara T.
    • Journal Title

      BMC Plant Biol.

      Volume: 14 Issue: 1 Pages: 57-57

    • DOI

      10.1186/1471-2229-14-57

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Uses of acidic hot spring eukaryotic algae: pure and applied sciences2017

    • Author(s)
      宮城島進也
    • Organizer
      光合成科学:エネルギーとバイオマス
    • Place of Presentation
      東京工業大学
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 真核細胞と葉緑体の分裂同調化機構2017

    • Author(s)
      宮城島進也
    • Organizer
      真核細胞のオルガネラ研究最前線
    • Place of Presentation
      大阪大学
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 宿主細胞と共生細胞の分裂同調化によるオルガネラ成立機構2016

    • Author(s)
      宮城島 進也
    • Organizer
      共生・寄生生物学シンポジウム
    • Place of Presentation
      つくば市
    • Year and Date
      2016-03-05
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Creation of heat and acid tolerant algae toward high biomass production2016

    • Author(s)
      Miyagishima, S.
    • Organizer
      IMBC2016
    • Place of Presentation
      Baltimore, USA
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Creation of heat and acid tolerant algae toward high biomass production2015

    • Author(s)
      Shin-ya Miyagishima
    • Organizer
      PACIFICHEM2015
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2015-12-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 真核藻類におけるエネルギー代謝と細胞周期進行の関係2015

    • Author(s)
      宮城島 進也
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      新潟市
    • Year and Date
      2015-09-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] 単細胞紅藻Cyanidioschyzon におけるエネルギー変換と細胞周期進行の関係2014

    • Author(s)
      宮城島進也,藤原崇之,墨谷暢子,恵良厚子
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      神奈川県川崎市(明治大学)
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Coordinating mechanisms of eukaryotic cell and chloroplast proliferation2014

    • Author(s)
      Miyagishima, S.
    • Organizer
      ESF-EMBO symposium, Biology of plastids-towards a blueprint for synthetic organelles
    • Place of Presentation
      Pultusk, Poland
    • Year and Date
      2014-06-22
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 単細胞紅藻Cyanidioschyzon merolaeにおける光合成と細胞成長・分裂の協調機構

    • Author(s)
      宮城島 進也
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道大学(札幌市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 真核細胞と葉緑体の協調増殖機構

    • Author(s)
      宮城島 進也
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学(富山市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Creation of heat and acid tolerant algae toward high biomass production

    • Author(s)
      Shin-ya Miyagishima
    • Organizer
      1st Korea-Japan Microalgae Symposium
    • Place of Presentation
      KAIST (Daejeon, Korea)
    • Related Report
      2013 Annual Research Report
    • Invited

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Published: 2013-05-15   Modified: 2019-07-29  

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