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Next generation sociogenomics: Genomic basis underlying the sociality in termites

Research Project

Project/Area Number 25251041
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Ecology/Environment
Research InstitutionThe University of Tokyo (2017-2018)
Hokkaido University (2013-2016)

Principal Investigator

Miura Toru  東京大学, 大学院理学系研究科(理学部), 教授 (00332594)

Co-Investigator(Kenkyū-buntansha) 前川 清人  富山大学, 大学院理工学研究部(理学), 准教授 (20345557)
重信 秀治  基礎生物学研究所, 生物機能解析センター, 特任准教授 (30399555)
Project Period (FY) 2013-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥46,020,000 (Direct Cost: ¥35,400,000、Indirect Cost: ¥10,620,000)
Fiscal Year 2017: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2016: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2015: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2014: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2013: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Keywordsシロアリ / 社会性 / ゲノミクス / DNAメチル化 / カースト / 遺伝子発現 / 形態形成因子 / トランスクリプトーム / ゲノム / ソシオゲノミクス / カースト分化 / コミュニケーション / RNA-seq / 兵隊 / 分化経路 / 個体間相互作用 / フェロモン / 形態形成
Outline of Final Research Achievements

In this research project, using recent techniques of next-generations sequencing techniques, we carried out the researches on a social insect, termites, focusing on the genomic basis underlying the highly sofisticated sociality. Based on the results obtained by transcriptomic and gene expression analyses, we successfully identified the genes responsible for the soldier caste-specific genes that are responsible for the mandibular morphogenesis. Furthremore, the comparisons among termite species revealed that, in the ancectral lineage of termites, the ratio of non-synonimous substitutions in many genes increased, suggesting that the positive natural selection was suggested to have occurred. In addition, it was also revealed that there is a negative correlation between methylation levels of genes and caste-biased gene expressions, and that the gene duplication might have facilitate the evolution of highly-sophisticated sociality seen in termites.

Academic Significance and Societal Importance of the Research Achievements

アリやシロアリなどの社会性昆虫では、発生過程において個体の形態や行動が明確に分化する「カースト分化」という現象を伴う高度な社会性(真社会性)が見られる。これまで、近代的な分子生物学的手法を用いた研究がアリ・ハチを中心に行われてきたが、シロアリの社会性については研究が後れており、社会性の原理の理解には至っていなかった。本研究では、シロアリのいくつかの種を用いて、トランスクリプトームおよびゲノム解析から、社会性に関わるゲノム基盤を解明しようとするもので、研究開始当初には想像だにしなかった多くの成果を得るに至っている。これらの研究成果から、動物の社会とは何かにつちえ答えが得られることが期待される。

Report

(6 results)
  • 2018 Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (43 results)

All 2019 2018 2017 2016 2015 2014 2013 Other

All Journal Article (24 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 24 results,  Open Access: 8 results,  Acknowledgement Compliant: 9 results) Presentation (13 results) (of which Int'l Joint Research: 2 results,  Invited: 7 results) Book (1 results) Remarks (5 results)

  • [Journal Article] Unique morphogenesis in the damp-wood termite: abscission of the stylus during female reproductive caste differentiation.2019

    • Author(s)
      Oguchi K, Miura T
    • Journal Title

      Zoological Science

      Volume: 36

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reduction of a nymphal instar in a dampwood termite: heterochronic shift in the caste differentiation pathways.2019

    • Author(s)
      Nii R, Oguchi K, Shinji J, Koshikawa S, Miura T
    • Journal Title

      EvoDevo

      Volume: 10

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Termite soldier mandibles are elongated by dachshund under hormonal and Hox gene controls2019

    • Author(s)
      Sugime Y, Oguchi K, Gotoh H, Hayashi Y, Matsunami M, Shigenobu S, Koshikawa S, Miura T
    • Journal Title

      Development

      Volume: 146 Issue: 5 Pages: 1-9

    • DOI

      10.1242/dev.171942

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Juvenile hormone as a physiological regulator mediating phenotypic plasticity in pancrustaceans2018

    • Author(s)
      Miura T
    • Journal Title

      Development, Growth & Differentiation

      Volume: 61 Issue: 1 Pages: 85-96

    • DOI

      10.1111/dgd.12572

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Caste-specific microRNA expression in termites: insights into soldier differentiation.2018

    • Author(s)
      Matsunami M, Nozawa M, Suzuki R, Toga K, Masuoka Y, Yamaguchi K, Maekawa K, Shigenobu S, Miura T
    • Journal Title

      Insect Molecular Biology

      Volume: 28 Issue: 1 Pages: 86-98

    • DOI

      10.1111/imb.12530

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The upregulation of juvenile hormone titers in female Drosophila melanogaster through mating experiences and host food occupied by egges and larvae.2017

    • Author(s)
      Sugime Y, Watanabe D, Yasuno Y, Shinada T, Miura T, Tanaka NK
    • Journal Title

      Zoological Science

      Volume: 34 Issue: 1 Pages: 52-57

    • DOI

      10.2108/zs160150

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The function of appendage patterning genes in mandible development of the sexually dimorphic stag beetle.2017

    • Author(s)
      Gotoh H, Zinna RA, Ishikawa Y, Miyakawa H, Ishikawa A, Sugime Y, Emlen DJ, Lavine LC, Miura T
    • Journal Title

      Developmental Biology

      Volume: 422 Issue: 1 Pages: 24-32

    • DOI

      10.1016/j.ydbio.2016.12.011

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Queen contact and among-worker interactions dually suppress worker brain dopamine as a potential regulator of reproduction in an ant.2017

    • Author(s)
      Shimoji, H., Aonuma,H., Miura, T., Tsuji, K., Sasaki, K., Okada, Y.
    • Journal Title

      Behavioral Ecology and Sociobiology

      Volume: 71 Issue: 2 Pages: 35-35

    • DOI

      10.1007/s00265-016-2263-3

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Male-specific molecular genetic markers in the Japanese subterranean termite Reticulitermes speratus (Isoptera: Rhinotermitidae).2017

    • Author(s)
      Hayashi Y, Oguchi K, Yamaguchi K, Kitade O, Maekawa K, Miura T, Shigenobu S
    • Journal Title

      Insectes Sciaux

      Volume: 64 Issue: 3 Pages: 357-364

    • DOI

      10.1007/s00040-017-0553-z

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Regulation of neotenic differentiation through direct physical contact in the damp-wood termite Hodotermopsis sjostedti.2017

    • Author(s)
      Shimoji H, Oguchi K, Hayashi Y, Hojo MK, Miura T
    • Journal Title

      Insectes Sociaux

      Volume: 64 Issue: 3 Pages: 393-401

    • DOI

      10.1007/s00040-017-0562-y

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Reproductive organ development along the caste differentiation pathways in the dampwood termite Hodotermopsis sjostedti.2016

    • Author(s)
      Oguchi K, Shimoji H, Hayashi Y, Miura T
    • Journal Title

      Insectes Sociaux

      Volume: 63 Issue: 4 Pages: 519-529

    • DOI

      10.1007/s00040-016-0495-x

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Functional transformation series and the evolutionary origin of novel forms: Evidence from a remarkable termite defensive organ.2016

    • Author(s)
      Kaji T, Keiler J, Bourguignon T, Miura T
    • Journal Title

      Evol Dev

      Volume: 18 Issue: 2 Pages: 78-88

    • DOI

      10.1111/ede.12179

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Sexual difference in juvenile-hormone titer in workers leads to sex-biased soldier differentiation in termites.2016

    • Author(s)
      Toga K, Hanmoto S, Suzuki R, Watanabe D, Miura T, Maekawa K
    • Journal Title

      J Insect Physiol

      Volume: 87 Pages: 63-70

    • DOI

      10.1016/j.jinsphys.2016.02.005

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Tyraminergic and octopaminergic modulation of defensive behavior in termite soldier.2016

    • Author(s)
      Ishikawa Y, Aonuma H, Sasaki K, Miura T
    • Journal Title

      PLoS One

      Volume: 11

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] High juvenile hormone titer and abdominal activation of the JH signaling may induce reproduction of termite neotenics.2015

    • Author(s)
      Saiki R, Gotoh H, Toga K, Miura T, Maekawa K
    • Journal Title

      Insect Molecular Biology

      Volume: 印刷中 Issue: 4 Pages: 432-441

    • DOI

      10.1111/imb.12169

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Social dominance and reproductive differentiation mediated by dopaminergic signaling in a queenless ant.2015

    • Author(s)
      Yasukazu Okada, Ken Sasaki, Satoshi Miyazaki, Hiroyuki Shimoji, Kazuki Tsuji and Toru Miura
    • Journal Title

      The Journal of Experimental Biology

      Volume: 218 Issue: 7 Pages: 1091-1098

    • DOI

      10.1242/jeb.118414

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Expansion of presoldier cuticle contributes to head elongation during soldier differentiation in termites.2015

    • Author(s)
      Sugime Y, Ogawa K, Watanabe D, Shimoji H, Koshikawa S, Miura T
    • Journal Title

      Sci Nat

      Volume: 102 Issue: 11-12 Pages: 71-71

    • DOI

      10.1007/s00114-015-1322-3

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The evolutionary history of termites as inferred from 66 mitochondrial genomes.2015

    • Author(s)
      Bourguignon T, Lo N, Cameron SL, Sobotnik J, Hayashi Y, Shigenobu S, Watanabe D, Roisin Y, Miura T, Evans TA
    • Journal Title

      Mol Biol Evol

      Volume: 32 Issue: 2 Pages: 406-421

    • DOI

      10.1093/molbev/msu308

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cockroach GABA-B receptor subtypes: Molecular characterization, pharmacological properties and tissue distribution.2015

    • Author(s)
      Blankenburg S, Balfanz S, Hayashi Y, Shigenobu S, Miura T, Baumann O, Baumann A, Blenau W
    • Journal Title

      Neuropharmacology

      Volume: 88 Pages: 134-144

    • DOI

      10.1016/j.neuropharm.2014.08.022

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sexually dimorphic body color is regulated by sex-specific expression of yellow gene in ponerine ant, Diacamma sp.2014

    • Author(s)
      Miyazaki, S., Okada, Y., Miyakawa, H., Tokuda, G., Cornette, R., Koshikawa, S., Maekawa, K. and Miura, T.
    • Journal Title

      PLOS ONE

      Volume: (In press) Issue: 3 Pages: e92875-e92875

    • DOI

      10.1371/journal.pone.0092875

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Social interactions affecting caste development through physiological actions in termites2014

    • Author(s)
      Watanabe D, Gotoh H, Miura T, Maekawa K
    • Journal Title

      Frontiers in Physiology

      Volume: 5 Pages: 127-127

    • DOI

      10.3389/fphys.2014.00127

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Aphid polyphenisms : trans-generational developmental regulation through viviparity2014

    • Author(s)
      Ogawa K, Miura T
    • Journal Title

      Frontiers in Physiology

      Volume: 5 Pages: 1-1

    • DOI

      10.3389/fphys.2014.00001

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Developmental Link between Sex and Nutrition ; doublesex Regulates Sex-Specific Mandible Growth via Juvenile Hormone Signaling in Stag Beetles.2014

    • Author(s)
      Gotoh H, Miyakawa H, Ishikawa A, Ishikawa Y, Sugime Y, Emlen DJ, Lavine LC, and Miura T
    • Journal Title

      PLOS Genetics

      Volume: 10 Issue: 1 Pages: e1004098-e1004098

    • DOI

      10.1371/journal.pgen.1004098

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction and characterization of normalized cDNA libraries by 454 pyrosequencing and estimation of DNA methylation levels in three distantly related termite specie2013

    • Author(s)
      Hayashi Y, Shigenobu S, Watanabe D, Toga K, Saiki R, Shimada K, Bourguignon T, Lo N, Hojo M, Maekawa K, Miura T
    • Journal Title

      PLOS ONE

      Volume: 8 Issue: 9 Pages: e76678-e76678

    • DOI

      10.1371/journal.pone.0076678

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] The making of the strongest: Developmental underpinnings of soldier differentiation in termites2018

    • Author(s)
      Miura T
    • Organizer
      International Union for the Study of Social Insects
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 発生プログラムの可塑的改変による新規表現型の獲得:シロアリの兵隊カースト分化を例に2018

    • Author(s)
      三浦徹
    • Organizer
      日本進化学会第20回大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 昆虫類における表現型可塑性の生物学2016

    • Author(s)
      三浦徹
    • Organizer
      種生物学会シンポジウム
    • Place of Presentation
      キロロトリビュートポートフォリオホテル北海道(北海道赤井川村)
    • Year and Date
      2016-12-02
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Evolutionary and developmental underpinnings of insect polyphenisms2016

    • Author(s)
      Miura T
    • Organizer
      International Congress of Zoology
    • Place of Presentation
      沖縄科学技術大学院大学(沖縄県恩納村)
    • Year and Date
      2016-11-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 昆虫における表現型多型:多型分化の生理発生基盤2015

    • Author(s)
      三浦 徹
    • Organizer
      日本動物学会 第86回新潟大会
    • Place of Presentation
      朱鷺メッセ
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] シロアリにおけるカースト分化の発生生理機構とその進化:社会生理学からソシオゲノミクスへ2014

    • Author(s)
      三浦徹
    • Organizer
      日本動物学会第85回大会
    • Place of Presentation
      東北大学
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Role of presoldier cuticle in the soldier-specific morphogenesis in termite2014

    • Author(s)
      Yasuhiro Sugime, Kota Ogawa, Dai Watanabe, Hiroyuki Shimoji, Shigeyuki Koshikawa, Toru Miura
    • Organizer
      International Union for the Study of Social Insects
    • Place of Presentation
      Cairns Convention Centre
    • Year and Date
      2014-07-14 – 2014-07-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Transcriptomics on social interactions in termites: Effects of caste presence2014

    • Author(s)
      Dai Watanabe, Masatoshi Matsunami, Yoshinobu Hayashi, Shuji Shigenobu, Toru Miura, Kiyoto Maekawa
    • Organizer
      International Union for the Study of Social Insects
    • Place of Presentation
      Cairns Convention Centre
    • Year and Date
      2014-07-14 – 2014-07-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Dachshundによるシロアリ兵隊の大顎伸長制御2014

    • Author(s)
      杉目康広,越川滋行,三浦徹
    • Organizer
      日本応用動物昆虫学会
    • Place of Presentation
      高知大学、高知県高知市
    • Related Report
      2013 Annual Research Report
  • [Presentation] The applications of RNAi for genes used for the phenotypic plasticity in the insect polyphenisms2014

    • Author(s)
      Toru Miura
    • Organizer
      International Symposium on RNAi and Genome Editing Methods
    • Place of Presentation
      徳島大学、徳島県徳島市
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] シロアリのカースト分化における社会生理機構に関するトランスクリプトミクス2014

    • Author(s)
      渡邊大,松波雅俊,林良信,重信秀治,三浦徹,前川清人
    • Organizer
      日本生態学会
    • Place of Presentation
      広島国際会議場、広島県広島市
    • Related Report
      2013 Annual Research Report
  • [Presentation] オオシロアリにおける兵隊特異的な唾液腺分化と発現タンパクの同定2013

    • Author(s)
      箕浦るん,渡邊大,三浦徹
    • Organizer
      日本昆虫学会
    • Place of Presentation
      北海道大学、北海道札幌市
    • Related Report
      2013 Annual Research Report
  • [Presentation] オオシロアリの前兵隊特異的なクチクラ形成と頭部伸長現象2013

    • Author(s)
      杉目康広,小川浩太,渡邊大,三浦徹
    • Organizer
      日本昆虫学会
    • Place of Presentation
      北海道大学、北海道札幌市
    • Related Report
      2013 Annual Research Report
  • [Book] 表現型可塑性の生物学:生態発生学入門2016

    • Author(s)
      三浦徹
    • Total Pages
      228
    • Publisher
      日本評論社
    • Related Report
      2016 Annual Research Report
  • [Remarks] 三浦研究室ホームページ

    • URL

      http://www.mmbs.s.u-tokyo.ac.jp/research/MiuraLab/index.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 北海道大学・大学院地球環境科学研究院・三浦研ホームページ

    • URL

      http://noah.ees.hokudai.ac.jp/~miu/miura_lab_japanese.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 東京大学・三崎臨海実験所・三浦研ホームページ

    • URL

      http://www.mmbs.s.u-tokyo.ac.jp/research/MiuraLab/index.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] Miura Lab

    • URL

      http://noah.ees.hokudai.ac.jp/%7Emiu/miura_lab.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] Miura Lab

    • URL

      http://noah.ees.hokudai.ac.jp/~miu/

    • Related Report
      2014 Annual Research Report

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Published: 2013-05-15   Modified: 2020-03-30  

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