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Elucidation of important breeding traits in tomato using a comprehensive mutant population

Research Project

Project/Area Number 25252008
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticultural science
Research InstitutionUniversity of Tsukuba

Principal Investigator

EZURA Hiroshi  筑波大学, 生命環境系, 教授 (00332552)

Co-Investigator(Kenkyū-buntansha) 加藤 一幾  岩手大学, 農学部, 准教授 (30613517)
有泉 亨  筑波大学, 生命環境系, 准教授 (70575381)
王 寧  筑波大学, 生命環境系, 助教 (90730193)
Research Collaborator TANASE Kyoko  
OKABE Yoshihiro  
ICHINO Takuji  
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥44,850,000 (Direct Cost: ¥34,500,000、Indirect Cost: ¥10,350,000)
Fiscal Year 2016: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2015: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2014: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2013: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Keywordsトマト / 重要形質 / 日持ち性 / 糖度 / 花成 / 日持ち
Outline of Final Research Achievements

The objectives of this study are to extending our knowledge on a molecular mechanism underlying important breeding traits including self-life and sugar accumulation of fruits and flowering, using a comprehensive mutant population based on Micro-Tom tomato which is a model cultivar of studying fruit development, and the effective mutant screening technology, TILLING. We have isolated and characterized new mutant alleles in genes reported as related genes which regulate such important breeding traits, while we have identified a novel gene accounting for sugar accumulation in tomato fruits. We have also demonstrated that some of new mutant alleles are useful for improving self-life of tomato fruits. These results will contribute to elucidate a molecular mechanism of important breeding traits.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (14 results)

All 2017 2016 2015 2014 2013

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Acknowledgement Compliant: 3 results) Presentation (11 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Favorable effects of the weak ethylene receptor mutation Sletr1-2 on postharvest fruit quality changes in tomatoes.2016

    • Author(s)
      Mubarok S, Okabe Y, Fukuda N, Ariizumi T, Ezura H
    • Journal Title

      Postharvest Biology and Technology

      Volume: 120 Pages: 1-9

    • DOI

      10.1016/j.postharvbio.2016.04.022

    • NAID

      120007129448

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Involvement of vacuolar processing enzyme SlVPE5 in post-transcriptional process of invertase in sucrose accumulation in tomato.2016

    • Author(s)
      Wang N, Duhita N, Ariizumi T, Ezura H
    • Journal Title

      Plant Physiology and Biochemistry

      Volume: 108 Pages: 71-78

    • DOI

      10.1016/j.plaphy.2016.06.037

    • NAID

      120007129262

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Potential use of a weak ethylene receptor mutant, Sletr1-2, as breeding material to extend fruit shelf life of tomato2015

    • Author(s)
      Syariful Mubarok, Yoshihiro Okabe, Naoya Fukuda, Tohru Ariizumi, Hiroshi Ezura
    • Journal Title

      J Agric Food Chem

      Volume: 63 Issue: 36 Pages: 7995-8007

    • DOI

      10.1021/acs.jafc.5b02742

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] TILLING法によるトマトの花成制御突然変異体の選抜および解析2017

    • Author(s)
      紫葉隆則・菊池太良・立澤文見・棚瀬京子・有泉 亨・王 寧・江面 浩・加藤一幾
    • Organizer
      園芸学会
    • Place of Presentation
      日本大学生物資源科学部
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] Genetic characterization of EMS-mutagenized Micro-Tom for study activities of functional genomics2016

    • Author(s)
      Ning Wang, Katsuki Nada, Di Liu, Miyako Kusano and Hiroshi Ezura
    • Organizer
      2th Asian Horticultural Congress
    • Place of Presentation
      Chengdu, China
    • Year and Date
      2016-09-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] Characterization of NOR mutant alleles isolated from EMS mutagenesis Micro-Tom2016

    • Author(s)
      Di Liu, Ning Wang, Kyouko Tanase, Nattiwong Pankasem, Haoting Chen, Miyako Kusano and Hiroshi Ezura
    • Organizer
      13th Solanaceae Conference
    • Place of Presentation
      University of California,Davis, USA
    • Year and Date
      2016-09-12
    • Related Report
      2016 Annual Research Report
  • [Presentation] Relevance of sucrose accumulation to the impact of vacuolar processing enzyme (SlVPE5) mediated invertase activation2015

    • Author(s)
      Ning Wang, Toru Ariizumi, Hiroshi Ezura
    • Organizer
      12 th Solanaceae Conference.
    • Place of Presentation
      Bordeaux, France
    • Year and Date
      2015-10-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] トマトの成長相転換における花成制御遺伝子の機能解析2015

    • Author(s)
      下山さとみ、及川純希、石上由佳、立澤文見、棚瀬京子、有泉亨、王寧、江面浩、加藤一幾
    • Organizer
      第33回日本植物細胞分子生物学会
    • Place of Presentation
      東京大学、東京都、文京区
    • Year and Date
      2015-08-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] Analysis of floral regulatory genes in a day-neutral tomato2014

    • Author(s)
      Junki Oikawa, Yuka Ishigami, Fumi Tatsuzawa, Tohru Ariizumi, Ning Wang, Hiroshi Ezura, Kazuhisa Kato
    • Organizer
      11th JSOL International Symposium on Solanaceae Genomics
    • Place of Presentation
      名古屋大学(愛知県名古屋市)
    • Year and Date
      2014-10-25 – 2014-10-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Characterization and Evaluation of F1 hybrid lines with SlETR1 mutant alleles2014

    • Author(s)
      Mubarok S, Y. Okabe, T. Ariizumi, N. Fukuda and H. Ezura
    • Organizer
      Tsukuba Global Science Week 2014
    • Place of Presentation
      Tsukuba, Japan、筑波大学
    • Year and Date
      2014-09-28 – 2014-09-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] 中性植物トマトの花成制御関連遺伝子の解析2014

    • Author(s)
      及川純希、石上由佳、立澤文見、有泉亨、王寧、江面浩、加藤一幾
    • Organizer
      第32回日本植物細胞分子生物学会(盛岡)大会
    • Place of Presentation
      いわて県民情報交流センター(岩手県盛岡市)
    • Year and Date
      2014-08-21 – 2014-08-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] A potential of Sletr1-2, a weak allele of tomato ethylene receptor mutant as a breeding material for improving shelf life of fruits2014

    • Author(s)
      Mubarok S, Y. Okabe, T. Ariizumi, N. Fukuda and H. Ezura
    • Organizer
      41st Annual Meeting of The Plant Growth Regulation Society of America & Japanese Society for the Chemical Regulation of Plants
    • Place of Presentation
      San Francisco, America
    • Year and Date
      2014-07-13 – 2014-07-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] A vacuolar processing enzyme (SlVPE5) dependent post-translational modification of acid invertase is required for sugar accumulation in tomato fruits2013

    • Author(s)
      Ning Wang, Duhita Narendra, Tohru Ariizumi, Hiroshi Ezura
    • Organizer
      10th International Symposium on Solanaceae Genomics
    • Place of Presentation
      Osaka Prefecture University, Japan
    • Related Report
      2013 Annual Research Report
  • [Presentation] 中性植物トマトの花成制御機構におけるSP5G遺伝子の機能解析2013

    • Author(s)
      加藤 一幾,石上 由佳,山田 瑛理,尾上 美咲,立澤 文見
    • Organizer
      第31回日本植物細胞分子生物学会(札幌)大会・シンポジウム
    • Place of Presentation
      北海道大学高等教育推進機構
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-15   Modified: 2019-07-29  

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