| Budget Amount *help |
¥45,760,000 (Direct Cost: ¥35,200,000、Indirect Cost: ¥10,560,000)
Fiscal Year 2016: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2015: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
Fiscal Year 2014: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2013: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
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| Outline of Final Research Achievements |
The objective of this project is to explore RNA silencing (RNAi) as the antiviral innate defense against fungal viruses in plant pathogenic filamentous fungi. Consequently, a great progress has been made, leading to breakthroughs: 1) Discovery of RNAi mediated antiviral defense that requires Dicer but not AGO in Cryphonectria parasitica, 2) Detection of inhibitory effects on RNA silencing activities against RNA and DNA viruses, and transgenes in Pyricularia oryzae, 3) Development of a screening protocol for host factors required for virus perception through transcription induction of key genes of RNAi upon virus infection in C. parasitica, 4) Identification of the SAGA complex as a transcriptional regulator of fungal RNA silencing. These findings will bring about a paradigm shift in RNAi. Fungi are now established as the third position next to animals and plants in antiviral defense research. Note that most of the obtained data were published in international, high-quality journals.
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