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Molecular mechanisms underlying nitrate-responsive gene expression in plants

Research Project

Project/Area Number 25252014
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionThe University of Tokyo

Principal Investigator

Yanagisawa Shuichi  東京大学, 生物生産工学研究センター, 教授 (20222359)

Project Period (FY) 2013-05-31 – 2017-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥45,890,000 (Direct Cost: ¥35,300,000、Indirect Cost: ¥10,590,000)
Fiscal Year 2016: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2015: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2014: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2013: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Keywords植物 / 硝酸シグナル / シグナル伝達 / 転写因子 / 遺伝子発現制御 / 植物栄養 / 硝酸応答 / 転写制御 / 窒素 / 遺伝子発現
Outline of Final Research Achievements

Nitrate-responsive gene expression plays a key role in adaptation to diverse nitrogen conditions in plants. We previously identified NLPs as transcription factors responsible for nitrate-responsive gene expression. In this study, we analyzed the molecular mechanisms underlying NLP-mediated and nitrate-responsive gene expression in Arabidopsis. By DNA microarray analysis, we found that NLP transcription factors are master regulators responsible for expression of most of nitrate-regulated genes in Arabidopsis. We also found that phosphorylation of NLP transcription factors is a key for nitrate signaling and induction of NLP activity.

Report

(5 results)
  • 2017 Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (44 results)

All 2018 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (12 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 12 results,  Acknowledgement Compliant: 6 results,  Open Access: 3 results) Presentation (27 results) (of which Int'l Joint Research: 5 results,  Invited: 9 results) Book (1 results) Remarks (2 results)

  • [Int'l Joint Research] ハーバード大学医学系大学院/カリフォルニア大学サンフランシスコ校(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Dept. of Molecular Biology, MGH/Genetics, Harvard Medical School(米国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Reduced expression of APUM24, encoding a novel rRNA processing factor, induces sugar-dependent nucleolar stress and altered sugar responses in Arabidopsis thaliana2018

    • Author(s)
      Maekawa, S., Ishida, T. and Yanagisawa, S.
    • Journal Title

      Plant Cell

      Volume: 30 Issue: 1 Pages: 209-227

    • DOI

      10.1105/tpc.17.00778

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nucleolar stress and sugar response in plants2018

    • Author(s)
      Maekawa, S. and Yanagisawa, S.
    • Journal Title

      Plant Signal Behav.

      Volume: - Issue: 3 Pages: e1442975-e1442975

    • DOI

      10.1080/15592324.2018.1442975

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Discovery of Nitrate-CPK-NLP signalling in central nutrient-growth networks.2017

    • Author(s)
      Liu, K., Niu, Y., Konishi, M., Wu, Y., Du, H., Sun Chung, H., Li, L., Boudsocq, M., McCormack, M., Maekawa, S., Ishida, T., Zhang, C., Shokat, K., *Yanagisawa, S., *Sheen, J.
    • Journal Title

      Nature

      Volume: 18;545 Issue: 7654 Pages: 311-316

    • DOI

      10.1038/nature22077

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular basis of the nitrogen response in plants.2017

    • Author(s)
      Ueda, Y., Konishi, M. and Yanagisawa, S.
    • Journal Title

      Soil Sci. Plant Nutr.

      Volume: 63 Issue: 4 Pages: 329-341

    • DOI

      10.1080/00380768.2017.1360128

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct transcriptional activation of BT genes by NLP transcription factors is a key component of the nitrate response in Arabidopsis2017

    • Author(s)
      Sato, T., Maekawa, S., Konishi, M., Yoshioka, N., Sasaki, Y., Maeda, H., Ishida, T., Kato, Y., Yamaguchi, J. and Yanagisawa, S.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 483 Issue: 1 Pages: 380-386

    • DOI

      10.1016/j.bbrc.2016.12.135

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The pre-rRNA processing complex in Arabidopsis includes two WD40-domain-containing proteins encoded by glucose-inducible genes and plant-specific proteins2016

    • Author(s)
      Tetsuya Ishida*, Shugo Maekawa*, Shuichi Yanagisawa
    • Journal Title

      Molecular Plant

      Volume: 9 Issue: 2 Pages: 312-315

    • DOI

      10.1016/j.molp.2015.11.003

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 15N-tracing studies on in vitro reactions of ferredoxin-dependent nitrite reductase and glutamate synthase using reconstituted electron donation systems2015

    • Author(s)
      Yoneyama, T., Fujimori, T., Yanagisawa, S., Hase, T. and Suzuki, A.
    • Journal Title

      Plant Cell Physiol.

      Volume: 56 Issue: 6 Pages: 1154-1161

    • DOI

      10.1093/pcp/pcv039

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Nitrite transport activity of a novel HPP family protein conserved in cyanobacteria and chloroplasts2014

    • Author(s)
      Maeda, S., Konishi, M., Yanagisawa, S. and Omata, T.
    • Journal Title

      Plant Cell Physiology

      Volume: 55 Issue: 7 Pages: 1311-1324

    • DOI

      10.1093/pcp/pcu075

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Emergence of a new step towards understanding the molecular mechanisms underlying nitrate-regulated gene expression2014

    • Author(s)
      Konishi, M. and Yanagisawa, S.
    • Journal Title

      Journal of Experimental Botany

      Volume: 65 Issue: 19 Pages: 5589-5600

    • DOI

      10.1093/jxb/eru267

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Transcription factors involved in controlling the expression of nitrate reductase genes in higher plants2014

    • Author(s)
      Yanagisawa, S.
    • Journal Title

      Plant Science

      Volume: 229 Pages: 167-171

    • DOI

      10.1016/j.plantsci.2014.09.006

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Characterization of metabolic states of Arabidopsis thaliana under diverse carbon and nitrogen nutrient conditions via targeted metabolomic analysis2014

    • Author(s)
      Sato, S. and Yanagisawa, S.
    • Journal Title

      Plant Cell Physiology

      Volume: 55 Issue: 2 Pages: 306-319

    • DOI

      10.1093/pcp/pct192

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 植物の硝酸シグナル応答機構 -NIN様タンパク質が硝酸シグナル応答を司る-2014

    • Author(s)
      小西美稲子、柳澤修一
    • Journal Title

      化学と生物

      Volume: 52 Pages: 421-421

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Phenome analysis of natural genetic variations with Arabidopsis ecotypes and rice cultivars: Visualization of different nutrient uptake ability2017

    • Author(s)
      Shuichi Yanagisawa
    • Organizer
      Global Engage’s 4t h Plant Genomics & Gene Editing Asia Congress
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The regulation of nutrient starvation-induced leaf senescence by light signaling2017

    • Author(s)
      Yasuhito Sakuraba
    • Organizer
      East Asian Symposium on Senescence and Chronobiology in Plants
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Ca2+シグナリングを介した硝酸シグナル伝達 のメカニズム2017

    • Author(s)
      小西美稲子
    • Organizer
      第3回 植物の栄養研究会
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Nutrient uptake-based assessment of genetic variation of nitrogen and phosphorus response in rice2017

    • Author(s)
      Yoshiaki Ueda, Shuichi Yanagisawa
    • Organizer
      International Plant Nutrition Colloquium - IPNC 2017
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] P欠乏による硝酸イオン吸収抑制の分子機構2017

    • Author(s)
      小西美稲子、前田佳栄、木羽隆敏、柳澤修一
    • Organizer
      日本土壌肥料学会2017年度仙台大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] フィトクロムを介した赤色光シグナルによるリン酸獲得機構の制御2017

    • Author(s)
      櫻庭康仁、馬渕敦士、射場厚、柳澤修一
    • Organizer
      日本土壌肥料学会2017年度仙台大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 栄養素の取り込みを指標としたイネの窒素・リン飢餓応答性の品種間差の解析2017

    • Author(s)
      植田佳明、宮尾(徳富)光恵、栁澤修一
    • Organizer
      日本土壌肥料学会2017年度仙台大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] シロイヌナズナの硝酸応答を担うNLP転写因子群の機能解析2017

    • Author(s)
      沖津孝幸、小西美稲子、柳澤修一
    • Organizer
      日本土壌肥料学会2017年度仙台大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 根の形態形成への糖誘導性リボソーム生合成関連因子OLI2の関与2017

    • Author(s)
      前川修吾、柳澤修一
    • Organizer
      日本土壌肥料学会2017年度仙台大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] シロイヌナズナ高親和性硝酸イオン輸送体NRT2.1遺伝子のグルタミンによる発現抑制2017

    • Author(s)
      郭鵬程、小西美稲子、柳澤修一
    • Organizer
      日本土壌肥料学会2017年度仙台大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Physiological roles of the nitrate-responsive gene encoded Dof2.1 transcription factor in Arabidopsis2017

    • Author(s)
      Meng-na Zhuo, Yasuhito Sakuraba, Shuichi Yanagisawa
    • Organizer
      日本土壌肥料学会2017年度仙台大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 硝酸シグナルによるシロイヌナズナNAD+生合成制御の可能性の検討2017

    • Author(s)
      斉藤守秋、小西美稲子、柳澤修一
    • Organizer
      日本土壌肥料学会2017年度仙台大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 硝酸シグナルによるNLP転写因子の翻訳後修飾2016

    • Author(s)
      小西美稲子、前川修吾、石田哲也、柳澤修一
    • Organizer
      日本土壌肥料学会2016年度佐賀大会
    • Place of Presentation
      佐賀大学本庄キャンパス(佐賀、佐賀)
    • Year and Date
      2016-09-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 硝酸シグナル応答における転写因子群NIGTファミリーの役割2016

    • Author(s)
      前田佳栄、小西美稲子、木羽 隆敏、榊原 均、柳澤修一
    • Organizer
      日本土壌肥料学会2016年度佐賀大会
    • Place of Presentation
      佐賀大学本庄キャンパス(佐賀、佐賀)
    • Year and Date
      2016-09-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 植物の硝酸応答を担うNLP転写因子群の機能解析2016

    • Author(s)
      沖津孝幸、小西美稲子、柳澤修一
    • Organizer
      日本土壌肥料学会2016年度佐賀大会
    • Place of Presentation
      佐賀大学本庄キャンパス(佐賀、佐賀)
    • Year and Date
      2016-09-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 硝酸シグナル伝達と植物成長2016

    • Author(s)
      柳澤修一
    • Organizer
      日本植物学会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄、那覇)
    • Year and Date
      2016-09-16
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Molecular mechanism underlying regulation of the expression of a nitrate transporter gene NRT2.1 in Arabidopsis2016

    • Author(s)
      Yoshie Maeda, Mineko Konishi, Tomohiro Kurai, Naoya Sawaki, Minami Sugimoto, Shuichi Yanagisawa
    • Organizer
      植物生理学会
    • Place of Presentation
      岩手大学上田キャンパス(岩手、盛岡)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Interacting proteins of nitrate-responsive transcription factors NLP6 and NLP72016

    • Author(s)
      Mineko Konishi, Shuichi Yanagisawa
    • Organizer
      植物生理学会
    • Place of Presentation
      岩手大学上田キャンパス(岩手、盛岡)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] NLP transcription factors governing nitrate-responsive gene expression2016

    • Author(s)
      Yanagisawa, S.
    • Organizer
      Plant and Animal Genome XXIV Conference (PAG)
    • Place of Presentation
      San Diego, CA, USA
    • Year and Date
      2016-01-09
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] NLP transcription factors governing nitrate-responsive gene expression2016

    • Author(s)
      Yanagisawa, S.
    • Organizer
      The Plant and Animal Genome XXIV Conference
    • Place of Presentation
      San Diego, CA, USA
    • Year and Date
      2016-01-09
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 植物の硝酸シグナル応答:わかってきたことと未解明の問題.2015

    • Author(s)
      柳澤修一
    • Organizer
      土壌肥料学会シンポジウム「植物栄養の多面的解析と応用に向けて」
    • Place of Presentation
      京都、京都市、京都大学
    • Year and Date
      2015-09-09
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 窒素応答に関わる転写因子2015

    • Author(s)
      柳澤修一
    • Organizer
      植物栄養研究会
    • Place of Presentation
      東京、文京区、東京大学
    • Year and Date
      2015-09-04
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 気孔機能化に関わるSCAP1遺伝子のプロモーター解析2015

    • Author(s)
      森脇 宏介,祢冝 淳太郎,柳澤 修一, 射場 厚
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2013 Annual Research Report
  • [Presentation] 植物の高温ストレス応答の初期で働く転写因子HsfA1の活性制御機構の解析2015

    • Author(s)
      大濱直彦、溝井順哉、趙彗美、小泉慎也、草壁和也、高橋史憲、石田哲也、柳澤修一、篠崎一雄、篠崎和子
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2013 Annual Research Report
  • [Presentation] 植物の窒素応答2015

    • Author(s)
      柳澤修一
    • Organizer
      第3回ブラキボディウムワークショップ
    • Place of Presentation
      川崎
    • Year and Date
      2015-03-05
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 硝酸シグナル応答を制御するNIN/NLPファミリータンパク質の転写促進活性と進化的考察2014

    • Author(s)
      小西美稲子,鈴木渉、柳澤修一
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      川崎
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2013 Annual Research Report
  • [Presentation] 硝酸シグナル応答性転写因子による地上部と根の生長の制御2014

    • Author(s)
      小西美稲子、柳澤修一
    • Organizer
      日本土壌肥料学会 2014年度東京大会
    • Place of Presentation
      東京
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2013 Annual Research Report
  • [Book] apter 12: Structure and evolution of the plant Dof transcription factor family in Plant Transcription Factors. Evolutionary, Structural and Functional Aspects (Daniel H. Gonzalez, ed.)2015

    • Author(s)
      Yanagisawa, S.
    • Total Pages
      15
    • Publisher
      Elsevier/Academic Press
    • Related Report
      2015 Annual Research Report
  • [Remarks] 植物の栄養による生長制御のためのネットワークにおける硝酸イオン-CPK-NLPシグナル伝達の発見

    • URL

      http://www.a.u-tokyo.ac.jp/topics/2017/20170518-1.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 植物のリボソーム生合成因子APUM24の機能解明と糖によるリボソーム生合成の制御機構の発見

    • URL

      http://www.a.u-tokyo.ac.jp/topics/2017/20171222-1.html

    • Related Report
      2016 Annual Research Report

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Published: 2013-06-20   Modified: 2022-02-21  

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