| Budget Amount *help |
¥44,070,000 (Direct Cost: ¥33,900,000、Indirect Cost: ¥10,170,000)
Fiscal Year 2015: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2014: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2013: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
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| Outline of Final Research Achievements |
The purpose of this study is the development of an extracellular functional RNA production system using the marine bacterium Rhodovulum sulfidophilum. We had already succeeded a production of an RNA aptamer. In the present study, first, we could show the extracellular production of short hairpin RNAs those are known as strong inhibitors of gene expression. We also proceeded to basic study of this bacterium which is important for the safety assurance of this bacterium in the RNA drugs production in future. We discovered that this bacterium has the quorum sensing system and that the bacterium produces a new type of auto-inducer in the system. We also discovered the gene transfer agent of this bacterium which was thought to be a natural agent for horizontal gene transfer. Also, the total genome sequence of this bacterium has been completed using the next generation sequencer.
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