Microbial production of artificial and functional RNAs and the use of the RNA producing microorganism
Project/Area Number |
25252017
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied microbiology
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Research Institution | Toyohashi University of Technology |
Principal Investigator |
KIKUCHI Yo 豊橋技術科学大学, 研究推進アドミニストレーションセンター, 特命教授 (40273320)
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Co-Investigator(Kenkyū-buntansha) |
UMEKAGE SO 豊橋技術科学大学, 大学院工学研究科, 講師 (30419436)
OHYAMA TAKASHI 早稲田大学, 教育・総合科学学術院, 教授 (60268513)
AOKI TAKASHI 早稲田大学, ナノ・ライフ創新研究機構, 招聘研究員 (00051805)
|
Project Period (FY) |
2013-10-21 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥44,070,000 (Direct Cost: ¥33,900,000、Indirect Cost: ¥10,170,000)
Fiscal Year 2015: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2014: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2013: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
|
Keywords | 発酵 / 核酸 / 応用微生物学 / RNA生産 / RNAドラッグ / 細胞外核酸 / 光合成細菌 / 機能性RNA |
Outline of Final Research Achievements |
The purpose of this study is the development of an extracellular functional RNA production system using the marine bacterium Rhodovulum sulfidophilum. We had already succeeded a production of an RNA aptamer. In the present study, first, we could show the extracellular production of short hairpin RNAs those are known as strong inhibitors of gene expression. We also proceeded to basic study of this bacterium which is important for the safety assurance of this bacterium in the RNA drugs production in future. We discovered that this bacterium has the quorum sensing system and that the bacterium produces a new type of auto-inducer in the system. We also discovered the gene transfer agent of this bacterium which was thought to be a natural agent for horizontal gene transfer. Also, the total genome sequence of this bacterium has been completed using the next generation sequencer.
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Complete genome sequence of cyanobacterium Geminocystis sp. NIES-3708, which performs type II complementary chromatic acclimation2015
Author(s)
Yuu Hirose, Mitsunori Katayama, Yoshiyuki Ohtsubo, Naomi Misawa, Erica Iioka, Wataru Suda, Kenshiro Oshima, Mitsumasa Hanaoka, Kan Tanaka, Toshihiko Eki, Masahiko Ikeuchi, Yo Kikuchi, Makoto Ishida, and Masahira Hattori
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Journal Title
Genome Announcement
Volume: 3
Issue: 3
Pages: 1-2
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Complete genome sequence of cyanobacterium Geminocystis sp. NIES-3709 that harbors phycoerythrin-rich phycobilisome2015
Author(s)
Yuu Hirose, Mitsunori Katayama, Yoshiyuki Ohtsubo, Naomi Misawa, Erica Iioka, Wataru Suda, Kenshiro Oshima, Mitsumasa Hanaoka, Kan Tanaka, Toshihiko Eki, Masahiko Ikeuchi, Yo Kikuchi, Makoto Ishida, and Masahira Hattori
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Journal Title
Genome Announcement
Volume: 3
Issue: 2
Pages: 1-2
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The gene transfer agent-like particle of the marine phototrophic bacterium Rhodovulum sulfidophilum2015
Author(s)
Nagao, N., Yamamoto, J., Komatsu, H., Suzuki, H., Hirose, Y., Umekage, S., Ohyama, T., and Kikuchi, Y.
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Journal Title
Biochemistry and Biophysics Reports
Volume: 4
Pages: 369-374
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Complete genome sequence of cyanobacterium Geminocystis sp. NIES-3708, which performs type II complementary chromatic acclimation2015
Author(s)
Hirose, Y., Katayama, M., Ohtsubo, Y., Misawa, N., Iioka, E., Suda, W., Oshima, K., Hanaoka, M., Tanaka, K., Eki, T., Ikeuchi, M., Kikuchi, Y., Ishida, M., Hattori, M.
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Journal Title
Genome Announcements
Volume: 未定
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Complete genome sequence of cyanobacterium Geminocystis sp. NIES-3709 that harbors phycoerythrin-rich phycobilisome2015
Author(s)
Hirose, Y., Katayama, M., Ohtsubo, Y., Misawa, N., Iioka, E., Suda, W., Oshima, K., Hanaoka, M., Tanaka, K., Eki, T., Ikeuchi, M., Kikuchi, Y., Ishida, M., Hattori, M.
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Journal Title
Genome Announcements
Volume: 未定
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Complete genome sequence of Rhodovulum sulfidophilum DSM 2351, an extracellular nucleic acids producing bacterium2015
Author(s)
Nagao, N., Hirose, Y., Misawa, N., Ohtsubo, Y., Umekage, S., Kikuchi, Y
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Journal Title
Genome Announcements
Volume: 未定
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Short hairpin RNAs of designed sequences can be extracellularly produced by the marine bacterium <i>Rhodovulum sulfidophilum</i>2014
Author(s)
Nagao, N., Suzuki, H., Numano, R., Umekage, S., Kikuchi, Y.
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Journal Title
The Journal of General and Applied Microbiology
Volume: 60
Issue: 6
Pages: 222-226
DOI
NAID
ISSN
0022-1260, 1349-8037
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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