Molecular mechanisms of self-incompatibility in the Brassicaceae and the Solanaceae

• Project/Area Number: 25252021
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: TAKAYAMA Seiji 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (70273836)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥46,540,000 (Direct Cost: ¥35,800,000、Indirect Cost: ¥10,740,000); Fiscal Year 2015: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000); Fiscal Year 2014: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000); Fiscal Year 2013: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
• Keywords: 植物 / シグナル伝達 / 細胞間認識 / 生殖 / 自家不和合性 / アブラナ科 / ナス科

Outline of Final Research Achievements

This study aimed to reveal the molecular mechanisms of self-incompatibility in the Brassicaceae and the Solanaceae. In the Brassicaceae, SI is mediated by a "self (= same S-haplotype)" specific interaction between pollen ligand (SP11) and its stigmatic receptor (SRK). We found that calcium signaling mediated by glutamate receptor is the major downstream event of SI signaling that triggers rejection of self-pollen. In the Solanaceae, SI is mediated by the absence of "self" specific interaction between style ribonuclease (S-RNase) and pollen F-box proteins (SLFs). S-RNase exerts cytotoxicity inside self-pollen tube to inhibit its growth. We found in Petunia that 18 types of SLFs form SCF E3 ubiquitin ligase complexes and collaboratively recognize and detoxify entire suite of "non-self" S-RNases.

Research Products

• [Journal Article] Efficient expression of SRK intracellular domain by a modeling-based protein engineering. (2016)
  • Author(s): Murase K, Hirano Y, Takayama S, Hakoshima T.
  • Journal Title: Protein Expression and Purification Volume: 印刷中 Pages: 70-75
  • DOI: 10.1016/j.pep.2015.09.020
  • Peer Reviewed
• [Journal Article] Identification of reference genes for quantitative expression analysis using large scale RNA-seq data of Arabidopsis thaliana and model crop plants (2016)
  • Author(s): Kudo T, Sasaki Y, Terashima S, Matsuda-Imai N, Takano T, Saito M, Kanno M, Ozaki S, Suwabe K, Suzuki G, Watanabe M, Matsuoka M, Takayama S, Yano K
  • Journal Title: Genes Genet. Syst. Volume: 印刷中
  • NAID: 130005421205
  • Peer Reviewed
• [Journal Article] Self-incompatibility in plants (2015)
  • Author(s): 円谷徹之、久保健一、高山誠司
  • Journal Title: 生化学 Volume: 87 Issue: 3 Pages: 308-314
  • DOI: 10.14952/SEIKAGAKU.2015.870308
  • ISSN: 0037-1017
  • Year and Date: 2015-06-25
• [Journal Article] Calcium signalling mediates self-incompatibility response in the Brassicaceae (2015)
  • Author(s): Megumi Iwano, Kanae Ito, Sota Fujii, Mitsuru Kakita, Hiroko Asano-Shimosato, Motoko Igarashi, Pulla Kaothien-Nakayama, Tetsuyuki Entani, Asaka Kanatani, Masashi Takehisa, Masaki Tanaka, Kunihiko Komatsu, Hiroshi Shiba, Takeharu Nagai, Atsushi Miyawaki, Akira Isogai & Seiji Takayama
  • Journal Title: Nature Plants Volume: 1 Issue: 9 Pages: 15128-15128
  • DOI: 10.1038/nplants.2015.128
  • NAID: 120005716858
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Transcriptional characteristics and differences in Arabidopsis stigmatic papilla cells pre- and post-pollination. (2015)
  • Author(s): Matsuda, T., Matsushima, M., Nabemoto, M., Osaka, M., Sakazono, S., Masuko-Suzuki, H., Takahashi, H., Nakazono, M., Iwano, M., Takayama, S., Shimizu, K. K., Okumura, K., Suzuki, G., Watanabe, M., Suwabe, K.
  • Journal Title: Plant and Cell Physiology Volume: 56 Issue: 4 Pages: 663-673
  • DOI: 10.1093/pcp/pcu209
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Generation of transgenic <i>Linum perenne</i> by <i>Agrobacterium</i>-mediated transformation (2015)
  • Author(s): Murase, K., Sugai, Y., Hayashi, S., Suzuki, Y., Tsujii, K., Takayama, S.
  • Journal Title: Plant Biotechnology Volume: 32 Issue: 4 Pages: 349-352
  • DOI: 10.5511/plantbiotechnology.15.1109a
  • NAID: 130005115907
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] Self-Incompatibility in the Solanaceae: Ubiquitination-Mediated Self-/Non-Self-Discrimination (2015)
  • Author(s): 円谷徹之、久保健一、高山誠司
  • Journal Title: KAGAKU TO SEIBUTSU Volume: 53 Issue: 12 Pages: 826-833
  • DOI: 10.1271/kagakutoseibutsu.53.826
  • NAID: 130005284297
  • ISSN: 0453-073X, 1883-6852
• [Journal Article] Gene duplication and genetic exchange drive the evolution of S-RNase-based self-incompatibility in Petunia (2015)
  • Author(s): Ken-ichi Kubo, Timothy Paape, Masaomi Hatakeyama, Tetsuyuki Entani, Akie Takara, Kie Kajihara, Mai Tsukahara, Rie Shimizu-Inatsugi, Kentaro K. Shimizu, Seiji Takayama
  • Journal Title: Nature Plants Volume: 1 Issue: 1 Pages: 14005-14005
  • DOI: 10.1038/nplants.2014.5
  • NAID: 120005716849
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ubiquitin-proteasome-mediated degradation of S-RNase in a solanaceous cross-compatibility reaction (2014)
  • Author(s): Tetsuyuki Entani, Ken-ichi Kubo, Shin Isogai, Yoichiro Fukao, Masahiro Shirakawa, Akira Isogai, Seiji Takayama
  • Journal Title: Plant Journal Volume: 印刷中 Issue: 6 Pages: 1014-1021
  • DOI: 10.1111/tpj.12528
  • Peer Reviewed / Open Access
• [Journal Article] Ca^<2+>-activated reactive oxygen species production by Arabidopsis RbohH and RbohJ is essential for proper pollen tube tip growth (2014)
  • Author(s): Kaya, H., Nakajima, R., Iwano, M., Kanaoka, M., Kimura, S., Takeda, S., Kawarazaki, T., Senzaki, E., Hamamura, H., Higashiyama, T., Takayama, S., Abe, M., and Kuchitsu, K.
  • Journal Title: Plant Cell Volume: 26 (3) Issue: 3 Pages: 1069-1080
  • DOI: 10.1105/tpc.113.120642
  • Peer Reviewed
• [Journal Article] Physiological and Genetic Analysis of CO_2-Induced Breakdown of Self-Incompatibility in Brassica rapa (2014)
  • Author(s): Lao, X., Suwabe, K., Niikura, S., Kakita, M., Iwano, M., and Takayama, S.
  • Journal Title: J Exp Bot. Volume: 65 (4) Issue: 4 Pages: 939-951
  • DOI: 10.1093/jxb/ert438
  • Peer Reviewed
• [Journal Article] A pollen coat-inducible autoinhibited Ca^<2+>-ATPase expressed in stigmatic papilla cells is required for compatible pollination in the Brassicaceae (2014)
  • Author(s): Iwano, M., Igarashi, M., Tarutani, Y., Kaothien-Nakayama, P., Nakayama, H., Moriyama, H., Yakabe, R., Entani, T., Shimosato-Asano, H., Ueki, M., Tamiya, G., and Takayama, S
  • Journal Title: Plant Cell Volume: 26 (2) Issue: 2 Pages: 636-649
  • DOI: 10.1105/tpc.113.121350
  • Peer Reviewed
• [Journal Article] Involvement of MLPK pathway in intraspecies unilateral incompatibility regulated by a single locus with stigma and pollen factors (2013)
  • Author(s): Yoshinobu Takada, Takahiro Sato, Go Suzuki, Hiroshi Shiba, Seiji Takayama, Masao Watanabe
  • Journal Title: G3 (Bethesda) Volume: 3 Issue: 4 Pages: 719-726
  • DOI: 10.1534/g3.113.005892
  • Peer Reviewed
• [Journal Article] Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells from a Combination of Laser Microdissection and RNA Sequencing (2013)
  • Author(s): Osaka, M., Matsuda, T., Sakazono, S., Masuko-Suzuki, H., Maeda, S., Sewaki, M., Sone, M., Takahashi, H., Nakazono, M., Iwano, M., Takayama, S., Shimizu, K.K., Yano, K., Lim, Y.P., Suzuki, G., Suwabe, K., and Watanabe, M.
  • Journal Title: Plant Cell Physiol. Volume: 54 (11) Issue: 11 Pages: 1894-1906
  • DOI: 10.1093/pcp/pct133
  • Peer Reviewed
• [Presentation] ナス科植物ペチュニアのCullin1遺伝子群の同定と自家不和合性反応に関する機能解析 (2016)
  • Author(s): 久保健一、塚原麻伊、円谷徹之、村瀬浩司、高山誠司
  • Organizer: 第57回日本植物生理学会
  • Place of Presentation: 岩手大学上田キャンパス（岩手県、盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] アブラナ科植物において低分子RNAとその標的配列の多様性により複雑な階層的優劣性が制御される (2015)
  • Author(s): 和田七夕子、安田晋輔、柿崎智博、三浦栄子、樽谷芳明、高田美信、鈴木剛、渡辺正夫、高山誠司
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会（BMB2015）ワークショップ
  • Place of Presentation: 神戸ポートピアホテル（兵庫県、神戸市）
  • Year and Date: 2015-12-02
  • Invited
• [Presentation] ナス科植物ペチュニアのCullin1遺伝子群の同定と自家不和合性反応に関する機能解析 (2015)
  • Author(s): 久保健一、塚原麻伊、円谷徹之、村瀬浩司、高山誠司
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会（BMB2015）
  • Place of Presentation: 神戸国際展示場（兵庫県、神戸市）
  • Year and Date: 2015-12-01
• [Presentation] 自家不和合性の分子機構 (2015)
  • Author(s): 高山誠司
  • Organizer: 国立遺伝学研究所研究会「植物の生殖成長期の発生を制御する分子機構」
  • Place of Presentation: 国立遺伝学研究所（静岡県、三島市）
  • Year and Date: 2015-11-07
  • Invited
• [Presentation] Allelic variants of small RNA control dominance hierarchy among self-incompatibility alleles in Brassica rapa (2015)
  • Author(s): Takayama S
  • Organizer: International Syposium of Correlative Gene System "Establishing Next-Generation Genes"
  • Place of Presentation: 奈良春日野国際フォーラム甍（奈良県、奈良市）
  • Year and Date: 2015-05-28
  • Int'l Joint Research
• [Presentation] Diversification and evolution of self-incompatibility systems in plants (2015)
  • Author(s): Kubo K, Paape T, Hatakeyama M, Entani T, Shimizu-Inatsugi R, Shimizu KK, Takayama S
  • Organizer: International Syposium of Correlative Gene System "Establishing Next-Generation Genes"
  • Place of Presentation: 奈良春日野国際フォーラム甍（奈良県、奈良市）
  • Year and Date: 2015-05-28
  • Int'l Joint Research
• [Presentation] Trans-acting small RNA controlling the dominance hierarchy among self-incompatibility alleles in Brassica rapa (2015)
  • Author(s): Yasuda S, Wada Y, Kakizaki T, Miura E, Tarutani Y, Shimoda T, Takada Y, Suzuki G, Watanabe M, Takayama S
  • Organizer: International Syposium of Correlative Gene System "Establishing Next-Generation Genes"
  • Place of Presentation: 奈良春日野国際フォーラム甍（奈良県、奈良市）
  • Year and Date: 2015-05-28
  • Int'l Joint Research
• [Presentation] 遺伝子重複と遺伝子交換がS-RNase型自家不和合性の進化をドライブする (2015)
  • Author(s): 久保健一、Timothy Paape、畠山剛臣、円谷徹之、清水（稲継）理恵、清水健太郎、高山誠司
  • Organizer: 第56回日本植物生理学会
  • Place of Presentation: 東京農業大学（東京都世田谷区）
  • Year and Date: 2015-03-18
• [Presentation] Pollen-stiguma interaction in the Brassicaceae (2015)
  • Author(s): Sota Fujii、Meguni Iwano、Kanae Ito、Seiji Takayama
  • Organizer: 第56回日本植物生理学会シンポジウム「Molecular dissection of reproductive processes in plants」
  • Place of Presentation: 東京農業大学（東京都世田谷区）
  • Year and Date: 2015-03-16
  • Invited
• [Presentation] 植物の自家不和合性機構の多様性と進化 (2014)
  • Author(s): 久保健一、円谷徹之、岩野恵、高山誠司
  • Organizer: 第37回日本分子生物学会ワークショップ「動植物における生殖戦略とその分子基盤」
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-26
• [Presentation] アブラナ科植物の和合・不和合受粉過程における情報伝達系の解析 (2014)
  • Author(s): 岩野恵、伊藤花菜江、五十嵐元子、浅野（下里）裕子、高山誠司
  • Organizer: 第55回日本植物生理学会シンポジウム
  • Place of Presentation: 富山大学五福キャンパス（富山県富山市）
• [Presentation] Search for genes involved in cross- and self-pollination in the Brassicaceae (2014)
  • Author(s): Motoko Igarashi, Keiko Ohara, Megumi Iwano, Masahiro Fujita, Nori Kurata, Seiji Takayama
  • Organizer: 第55回日本植物生理学会
  • Place of Presentation: 富山大学五福キャンパス（富山県富山市）
• [Presentation] STEM トモグラフィーを用いたシロイヌナズナの受精過程の解析 (2014)
  • Author(s): 堀井真人、岩野恵、森稔幸、高山誠司
  • Organizer: 第55回日本植物生理学会
  • Place of Presentation: 富山大学五福キャンパス（富山県富山市）
• [Presentation] アブラナ科植物自家不和合性の分子メカニズム (2013)
  • Author(s): 岩野恵、伊藤花菜江、浅野（下里）裕子、五十嵐元子、岩尾康宏，高山誠司
  • Organizer: 第36回日本分子生物学会ワークショップ
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
• [Presentation] 植物の自家不和合性における自己非自己識別機構に関する研究
  • Author(s): 高山誠司
  • Organizer: 第84回日本農学大会
  • Place of Presentation: 東京大学山上会館（東京都文京区）
  • Invited
• [Presentation] Analysis of intracellular signaling pathway in Brassica self-incompatibility
  • Author(s): Kanae Ito, Megumi Iwano, Hiroko Shimosato-Asano, Mitsuru Kakita, Pulla Nakayama, Seiji Takayama
  • Organizer: 奈良先端未来開拓コロキウム "Signaling and Coordination in Plant Development"
  • Place of Presentation: 奈良先端科学技術大学院大学（奈良県生駒市）
• [Presentation] Molecular mechanism for S-RNase-based self incompatibility in Petunia
  • Author(s): Mai Tsukahara, Tetsuyuki Entani, Ken-ichi kubo, Kohji Murase,Megumi Iwano, Seiji Takayama
  • Organizer: 奈良先端未来開拓コロキウム "Signaling and Coordination in Plant Development"
  • Place of Presentation: 奈良先端科学技術大学院大学（奈良県生駒市）
• [Book] "Self-Incompatibility in the Brassicaceae" in "Sexual Reproduction in Animals and Plants" (2014)
  • Author(s): Megumi Iwano, Kanae Ito, Hiroko Shimosato-Asano, Kok Song Lai, Seiji Takayama
  • Total Pages: 480
  • Publisher: Springer, Heidelberg
• [Remarks] 奈良先端科学技術大学院大学バイオサイエンス研究科細胞間情報学研究室
  • URL: http://bsw3.naist.jp/takayama
• [Remarks] 奈良先端科学技術大学院大学バイオサイエンス研究科細胞間情報学研究室
  • URL: http://bsw3.naist.jp/takayama/
• [Remarks] 奈良先端科学技術大学院大学バイオサイエンス研究科 細胞間情報学研究室
  • URL: http://bsw3.naist.jp/takayama/