Molecular mechanisms of self-incompatibility in the Brassicaceae and the Solanaceae
Project/Area Number |
25252021
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied biochemistry
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Research Institution | Nara Institute of Science and Technology |
Principal Investigator |
TAKAYAMA Seiji 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (70273836)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥46,540,000 (Direct Cost: ¥35,800,000、Indirect Cost: ¥10,740,000)
Fiscal Year 2015: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2014: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2013: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
|
Keywords | 植物 / シグナル伝達 / 細胞間認識 / 生殖 / 自家不和合性 / アブラナ科 / ナス科 |
Outline of Final Research Achievements |
This study aimed to reveal the molecular mechanisms of self-incompatibility in the Brassicaceae and the Solanaceae. In the Brassicaceae, SI is mediated by a "self (= same S-haplotype)" specific interaction between pollen ligand (SP11) and its stigmatic receptor (SRK). We found that calcium signaling mediated by glutamate receptor is the major downstream event of SI signaling that triggers rejection of self-pollen. In the Solanaceae, SI is mediated by the absence of "self" specific interaction between style ribonuclease (S-RNase) and pollen F-box proteins (SLFs). S-RNase exerts cytotoxicity inside self-pollen tube to inhibit its growth. We found in Petunia that 18 types of SLFs form SCF E3 ubiquitin ligase complexes and collaboratively recognize and detoxify entire suite of "non-self" S-RNases.
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Report
(4 results)
Research Products
(35 results)
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[Journal Article] Identification of reference genes for quantitative expression analysis using large scale RNA-seq data of Arabidopsis thaliana and model crop plants2016
Author(s)
Kudo T, Sasaki Y, Terashima S, Matsuda-Imai N, Takano T, Saito M, Kanno M, Ozaki S, Suwabe K, Suzuki G, Watanabe M, Matsuoka M, Takayama S, Yano K
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Journal Title
Genes Genet. Syst.
Volume: 印刷中
NAID
Related Report
Peer Reviewed
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[Journal Article] Calcium signalling mediates self-incompatibility response in the Brassicaceae2015
Author(s)
Megumi Iwano, Kanae Ito, Sota Fujii, Mitsuru Kakita, Hiroko Asano-Shimosato, Motoko Igarashi, Pulla Kaothien-Nakayama, Tetsuyuki Entani, Asaka Kanatani, Masashi Takehisa, Masaki Tanaka, Kunihiko Komatsu, Hiroshi Shiba, Takeharu Nagai, Atsushi Miyawaki, Akira Isogai & Seiji Takayama
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Journal Title
Nature Plants
Volume: 1
Issue: 9
Pages: 15128-15128
DOI
NAID
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Transcriptional characteristics and differences in Arabidopsis stigmatic papilla cells pre- and post-pollination.2015
Author(s)
Matsuda, T., Matsushima, M., Nabemoto, M., Osaka, M., Sakazono, S., Masuko-Suzuki, H., Takahashi, H., Nakazono, M., Iwano, M., Takayama, S., Shimizu, K. K., Okumura, K., Suzuki, G., Watanabe, M., Suwabe, K.
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Journal Title
Plant and Cell Physiology
Volume: 56
Issue: 4
Pages: 663-673
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Generation of transgenic <i>Linum perenne</i> by <i>Agrobacterium</i>-mediated transformation2015
Author(s)
Murase, K., Sugai, Y., Hayashi, S., Suzuki, Y., Tsujii, K., Takayama, S.
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Journal Title
Plant Biotechnology
Volume: 32
Issue: 4
Pages: 349-352
DOI
NAID
ISSN
1342-4580, 1347-6114
Related Report
Peer Reviewed
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[Journal Article] Ca^<2+>-activated reactive oxygen species production by Arabidopsis RbohH and RbohJ is essential for proper pollen tube tip growth2014
Author(s)
Kaya, H., Nakajima, R., Iwano, M., Kanaoka, M., Kimura, S., Takeda, S., Kawarazaki, T., Senzaki, E., Hamamura, H., Higashiyama, T., Takayama, S., Abe, M., and Kuchitsu, K.
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Journal Title
Plant Cell
Volume: 26 (3)
Issue: 3
Pages: 1069-1080
DOI
Related Report
Peer Reviewed
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[Journal Article] Physiological and Genetic Analysis of CO_2-Induced Breakdown of Self-Incompatibility in Brassica rapa2014
Author(s)
Lao, X., Suwabe, K., Niikura, S., Kakita, M., Iwano, M., and Takayama, S.
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Journal Title
J Exp Bot.
Volume: 65 (4)
Issue: 4
Pages: 939-951
DOI
Related Report
Peer Reviewed
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[Journal Article] A pollen coat-inducible autoinhibited Ca^<2+>-ATPase expressed in stigmatic papilla cells is required for compatible pollination in the Brassicaceae2014
Author(s)
Iwano, M., Igarashi, M., Tarutani, Y., Kaothien-Nakayama, P., Nakayama, H., Moriyama, H., Yakabe, R., Entani, T., Shimosato-Asano, H., Ueki, M., Tamiya, G., and Takayama, S
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Journal Title
Plant Cell
Volume: 26 (2)
Issue: 2
Pages: 636-649
DOI
Related Report
Peer Reviewed
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[Journal Article] Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells from a Combination of Laser Microdissection and RNA Sequencing2013
Author(s)
Osaka, M., Matsuda, T., Sakazono, S., Masuko-Suzuki, H., Maeda, S., Sewaki, M., Sone, M., Takahashi, H., Nakazono, M., Iwano, M., Takayama, S., Shimizu, K.K., Yano, K., Lim, Y.P., Suzuki, G., Suwabe, K., and Watanabe, M.
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Journal Title
Plant Cell Physiol.
Volume: 54 (11)
Issue: 11
Pages: 1894-1906
DOI
Related Report
Peer Reviewed
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