Development of the 3rd-generation resistant cultivars against pine wood nematodes based on the exhaustive transcriptome information

Research Project

Project/Area Number	25252028
Research Category	Grant-in-Aid for Scientific Research (A)
Allocation Type	Single-year Grants
Section	一般
Research Field	Forest science
Research Institution	Kyushu University
Principal Investigator	Shiraishi Susumu 九州大学, (連合)農学研究科(研究院), 教授 (70226314)
Co-Investigator(Kenkyū-buntansha)	Mori Yasuhiro 福岡県農林業総合試験場資源活用研究センター, 専門研究員 (20558613) Gotoh Eiji 九州大学, 大学院農学研究院, 助教 (90614256)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥41,080,000 (Direct Cost: ¥31,600,000、Indirect Cost: ¥9,480,000) Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2014: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000) Fiscal Year 2013: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
Keywords	マツ材線虫病 / 樹病 / 抵抗性遺伝子 / 森林保護学 / 林木育種学 / 育種学
Outline of Final Research Achievements	A more economic and efficient SuperSAGE was established for exhaustive transcriptome analyses of a pile of samples, that is, homopolymer extension by Terminal deoxynucleotidyl transferase was introduced for the double strand cDNA synthesis. Also a dual labeling system was used in the sequencing with a NGS. The exhaustive transcriptome analyses using the modified SuperSAGE revealed that (1) pines attached by pine wood nematode expressed associated genes immediately after the infection, and (2) there are many differences in the gene expression among the resistant clones of Japanese black pine.