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Molecular biological study on next-generation utilization of carbon monoxide

Research Project

Project/Area Number 25252038
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Aquatic life science
Research InstitutionKyoto University

Principal Investigator

Sako Yoshihiko  京都大学, (連合)農学研究科(研究院), 教授 (60153970)

Co-Investigator(Kenkyū-buntansha) YOSHIDA TAKASHI  京都大学, 農学研究科, 准教授 (80305490)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥47,840,000 (Direct Cost: ¥36,800,000、Indirect Cost: ¥11,040,000)
Fiscal Year 2015: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2014: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2013: ¥30,810,000 (Direct Cost: ¥23,700,000、Indirect Cost: ¥7,110,000)
Keywords一酸化炭素資化性菌 / 水素生産 / COデヒドロゲナーゼ / CO代謝 / 次世代エネルギー / CO2固定 / ゲノム解析 / メタゲノム解析 / 一酸化炭素 / 海洋微生物 / CO資化性菌 / メタゲノム
Outline of Final Research Achievements

The aim of this study is to understand the genetic, physiological and ecological basis of thermophilic carboxydotrophs that can grow on carbon monoxide for construction of a modern sustainable low carbon technology. Main results of this study are as follows. We successfully isolated novel thermophilic carboxydotrophs from hydrothermal environments and revealed diverse CO metabolism through de novo genomic and transcriptional analysis. In addition, we demonstrated that various carboxydotrophs widely distributed domestic hydrothermal environments and unveiled the microbial community structures of those environments in detail.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (20 results)

All 2016 2015 2014 2013 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 1 results) Presentation (14 results) Remarks (1 results)

  • [Journal Article] Detection of anaerobic carbon monoxide-oxidizing thermophiles in hydrothermal environments2015

    • Author(s)
      Yoneda Y, Kano S, Yoshida T, Ikeda E, Fukuyama Y, Omae K, Kimura-Sakai S, Daifuku T, Watanabe T and Sako Y
    • Journal Title

      FEMS Microbiol. Ecol.

      Volume: 91 Issue: 9 Pages: 1-9

    • DOI

      10.1093/femsec/fiv093

    • NAID

      120005715337

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Over-expression of carbon monoxide dehydrogenase-I with an accessory protein co-exnression : a key enzyme for carbon dioxide reduction.2014

    • Author(s)
      Inoue T., Takao K., Fukuyama Y., Yoshida T. and Sako Y
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: (In press) Issue: 4 Pages: 582-587

    • DOI

      10.1080/09168451.2014.890027

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cysteine 295 indirectly affects Ni coordination of carbon monoxide dehydrogenase-II C-cluster.2013

    • Author(s)
      Takahiro Inoue, Kyosuke Takao, Takashi Yoshida, Kei Wada, Takashi Daifuku, Yasuko Yoneda, Keiichi Fukuyama, & Yoshihiko Sako.
    • Journal Title

      Biochemical and Biophysical Researcr Communications

      Volume: 441 Issue: 1 Pages: 13-17

    • DOI

      10.1016/j.bbrc.2013.09.143

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome variation in the hyperthermophilic archaeon Aeropyrum.2013

    • Author(s)
      Daifuku, T., Yoshida, T., and Sako, Y.
    • Journal Title

      Mobile Genetic Elements

      Volume: 3巻 Issue: 5 Pages: e26833-e26833

    • DOI

      10.4161/mge.26833

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bacterial and Archaeal Diversity in an Iron-Rich Coastal Hydrothermal Field in Yamagawa, Kagoshima, Japan2013

    • Author(s)
      Kawaichi S., Ito N., Yoshida T. and Sako Y.
    • Journal Title

      Microbes and Environments

      Volume: 28 Issue: 4 Pages: 405-413

    • DOI

      10.1264/jsme2.ME13048

    • NAID

      130003382016

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] オーミックス解析を用いた一酸化炭素 (CO) 資化性菌のCOによる転写変動解析2016

    • Author(s)
      福山宥斗、大前公保、米田恭子、吉田天士、左子芳彦
    • Organizer
      平成28年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Genomic adaptation of the thermophilic hydrogenogenic carboxydotrophic bacterium from marine sediment, Calderihabitans maritimus KKC1, driven by carbon monoxide dehydrogenases2015

    • Author(s)
      Omae, K., Yoneda, Y., Fukuyama, Y., Daifuku, T., Yoshida, T. and Sako, Y.
    • Organizer
      2015日本微生物生態学会第30回
    • Place of Presentation
      土浦亀城プラザ 茨城
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] RNA-Seq provides insights into the novel carbon monoxide metabolism in Carboxydothermus pertinax2015

    • Author(s)
      Fukuyama, Y., Ohmae, K., Yoneda, Y., Takao, K., Honda, T., Yoshida, T., and Sako, Y.
    • Organizer
      2015日本微生物生態学会第30回
    • Place of Presentation
      土浦亀城プラザ 茨城
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 次世代シーケンサーを用いた高温強酸温泉における微生物群集構造解析2015

    • Author(s)
      見世健太、池田栄太朗、大前公保、福山宥斗、吉田天士、左子芳彦
    • Organizer
      平成27年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学 品川キャンパス
    • Year and Date
      2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 水素生成型一酸化炭素資化性菌Calderihabitans maritimus KKC1と酢酸生成菌Moorella spp.の比較ゲノム解析2015

    • Author(s)
      大前公保、米田恭子、福山宥斗、吉田天士、左子芳彦
    • Organizer
      平成27年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学 品川キャンパス
    • Year and Date
      2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 網羅的転写解析による一酸化炭素 (CO) 資化性菌のCO代謝機構の解明2015

    • Author(s)
      福山宥斗、大前公保、本田貴史、池田栄太朗、吉田天士、左子芳彦
    • Organizer
      平成27年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学 品川キャンパス
    • Year and Date
      2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 水素生成型一酸化炭素資化性好熱細菌Calderihabitans maritimus KKC1のゲノム解析2015

    • Author(s)
      大前公保、米田恭子、福山宥斗、吉田天士、左子芳彦
    • Organizer
      第9回日本ゲノム微生物学会年会
    • Place of Presentation
      神戸大学 六甲第2キャンパス
    • Year and Date
      2015-03-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] 好熱菌Carboxydothermus pertinaxの一酸化炭素と共役する新規水素生成機構の探索2014

    • Author(s)
      福山宥斗、大前公保、池田栄太朗、吉田天士、左子芳彦
    • Organizer
      平成26年度日本水産学会近畿支部例会
    • Place of Presentation
      京都大学 農学部
    • Year and Date
      2014-11-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 熱水環境中の一酸化炭素資化性好熱菌の分子生態学的研究2014

    • Author(s)
      池田栄太朗・米田恭子・吉田天士・福山宥斗・左子芳彦
    • Organizer
      環境微生物系学会合同大会2014
    • Place of Presentation
      アクトシティ浜松
    • Year and Date
      2014-10-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] 海洋性水素生成型一酸化炭素資化性好熱細菌Calderihabitans maritimus KKC1のゲノム解析2014

    • Author(s)
      大前公保、吉田天士、米田恭子、福山宥斗、左子芳彦
    • Organizer
      平成26年度日本水産学会秋季大会
    • Place of Presentation
      九州大学
    • Year and Date
      2014-09-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] CO資化性菌のCODH活性中心におけるNi配位様式に関する研究2013

    • Author(s)
      高尾郷介、井上喬裕、吉田天士、左子芳彦
    • Organizer
      平成25年度日本水産学会秋季大会
    • Place of Presentation
      三重大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 熱水環境中の一酸化炭素資化性好熱Carboxydothermusの分子生態学的研究2013

    • Author(s)
      鹿野早苗、米田恭子、吉田天士、北村崇行、大福高史、井上喬裕、福山宥斗、高尾郷介
    • Organizer
      第29回微生物生態学会大会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 一酸化炭素資化性好熱菌Carboxydothermus pertinax のCO代謝2013

    • Author(s)
      福山宥斗、米田恭子、高尾郷介、大福高史、井上喬裕、吉田天士、左子芳彦
    • Organizer
      第29回微生物生態学会大会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 一酸化炭素資化性好熱菌Carboxydothermus属のゲノム解析

    • Author(s)
      高尾郷介、吉田天士、米田恭子、大福高史、福山宥斗、左子芳彦
    • Organizer
      微生物科学研究の多様性と新展開シンポジウム
    • Place of Presentation
      京都大学
    • Related Report
      2013 Annual Research Report
  • [Remarks] 海洋分子微生物学研究室ホームページ

    • URL

      http://www.microbiology.marine.kais.kyoto-u.ac.jp/

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2013-05-15   Modified: 2019-07-29  

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