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Prediction of deterioration rate of fruits and vegetables on the basis of visualized localization of enzymatic activity for respiration

Research Project

Project/Area Number 25252045
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Agricultural environmental engineering/Agricultural information engineering
Research InstitutionThe University of Tokyo

Principal Investigator

Makino Yoshio  東京大学, 大学院農学生命科学研究科(農学部), 准教授 (70376565)

Co-Investigator(Kenkyū-buntansha) 野口 航  東京薬科大学, 生命科学部, 教授 (80304004)
Co-Investigator(Renkei-kenkyūsha) OSHITA Seiichi  東京大学, 大学院農学生命科学研究科, 特任教授 (00115693)
Research Collaborator TSUKADA Masato  日本電気株式会社, データサイエンス研究所, 主任研究員
PARK Bosoon  米国農務省, Richard B. Russell Research Center, Lead scientist
Project Period (FY) 2013-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥47,580,000 (Direct Cost: ¥36,600,000、Indirect Cost: ¥10,980,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2013: ¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000)
Keywords分光分析 / 農業工学 / 農林水産物 / 細胞・組織 / 酵素
Outline of Final Research Achievements

We studied the distribution of deterioration rate of cruciferous vegetable flower buds and the relationship between respiratory enzymes. By the light sensing, the nondestructive estimation accuracy of the yellowing rate distribution of broccoli flower buds was influenced by light reflectance around 750 nm, which was confirmed to be light reflection due to chlorophyll fluorescence. Nondestructive estimation of the browning rate distribution of cauliflower flower buds was possible with screening level accuracy.
The yellowing rate of the broccoli flower bud was deeply related to the increase / decrease of one type of respiratory enzyme "Alternative Oxidase (AOX)", and the yellowing was suppressed under the environmental condition where the increase in AOX amount was suppressed. From this, it became clear that respiratory enzymes influence the deterioration rate control of cruciferous vegetables.

Report

(6 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (35 results)

All 2017 2016 2015 2014 2013 Other

All Int'l Joint Research (3 results) Journal Article (5 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 4 results) Presentation (25 results) (of which Int'l Joint Research: 5 results,  Invited: 3 results) Book (2 results)

  • [Int'l Joint Research] アメリカ農務省(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] アメリカ農務省(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] アメリカ農務省(米国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Influence of a Modified Atmosphere on the Induction and Activity of Respiratory Enzymes in Broccoli Florets during the Early Stage of Postharvest Storage2017

    • Author(s)
      Wang Hsiao-Wen、Makino Yoshio、Inoue Jun、Maejima Kensaku、Funayama-Noguchi Sachiko、Yamada Takeshi、Noguchi Ko
    • Journal Title

      Journal of Agricultural and Food Chemistry

      Volume: 65 Issue: 39 Pages: 8538-8543

    • DOI

      10.1021/acs.jafc.7b02318

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A grading method for mangoes on the basis of peel color measurement using a computer vision system2016

    • Author(s)
      Makino Y., Goto K., Oshita S., Sato A., Tsukada M.
    • Journal Title

      Agricultural Sciences

      Volume: 7 Issue: 06 Pages: 327-334

    • DOI

      10.4236/as.2016.76033

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Objective Evaluation of External Quality of Broccoli Heads Using a Computer Vision System2016

    • Author(s)
      Makino Y., Wakatsuki A., Amino G., Oshita S., Sato A., Tsukada M.
    • Journal Title

      Japan Journal of Food Engineering

      Volume: 17 Issue: 4 Pages: 107-113

    • DOI

      10.11301/jsfe.17.107

    • NAID

      130006152059

    • ISSN
      1345-7942, 1884-5924
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 光センシングによる農畜産物の非破壊品質評価2015

    • Author(s)
      牧野義雄
    • Journal Title

      調理食品と技術

      Volume: 21 Pages: 55-65

    • NAID

      40020642033

    • Related Report
      2015 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] Nondestructive Evaluation of Anthocyanin Concentration and Soluble Solid Content at the Vine and Blossom Ends of Green Mature Mangoes during Storage by Hyperspectral Spectroscopy2015

    • Author(s)
      Makino Y, Isami A., Suhara T., Goto K., Oshita S., Kawagoe Y., Kuroki S., Purwanto Y.A., Ahmad U., Sutrisno
    • Journal Title

      Food Science and Technology Research

      Volume: 21 Issue: 1 Pages: 59-65

    • DOI

      10.3136/fstr.21.59

    • NAID

      130005065048

    • ISSN
      1344-6606, 1881-3984
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] デジタルカメラによる青果物外観品質の客観的判定に関する研究2017

    • Author(s)
      牧野義雄,吉村正俊,外13名
    • Organizer
      2017国際食品工業展アカデミックプラザ研究発表会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Dynamic changes in activity and amount of respiratory enzymes in a broccoli floret stored under modified atmosphere2017

    • Author(s)
      Wang H.W., Makino Y., Inoue J., Oshita S., Maejima K., Funayama-Noguchi S., Noguchi K.
    • Organizer
      XII International Controlled and Modified Atmosphere Research Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of gas composition on respiratory enzyme activity in broccoli after harvest2017

    • Author(s)
      Wang H.W., Makino Y., Inoue J., Oshita S., Maejima K., Funayama-Noguchi S., Noguchi K.
    • Organizer
      日本包装学会第26回年次大会研究発表会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 低O2高CO2環境が収穫後ブロッコリーの呼吸酵素活性の経時変化に及ぼす影響2017

    • Author(s)
      井上潤, 王孝ウェン, 牧野義雄, 吉村正俊, 前島健作, 舟山幸子, 野口航
    • Organizer
      第76回農業食料工学会年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 分光分析を用いたカリフラワーの貯蔵中における酵素的褐変の出現予測2016

    • Author(s)
      牧野義雄,西本成潤,大下誠一
    • Organizer
      日本生物環境工学会2016年金沢大会
    • Place of Presentation
      金沢工業大学(石川県野々市市)
    • Year and Date
      2016-09-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] The effect of micro-perforated pouch on respiratory enzyme of broccoli2016

    • Author(s)
      Wang H. W., Makino Y., Oshita S., Maejima K., Funayama S., Noguchi K.
    • Organizer
      農業食料工学会関東支部第52回年次大会
    • Place of Presentation
      筑波大学(茨城県つくば市)
    • Year and Date
      2016-08-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] 低O2高CO2環境がブロッコリー細胞内呼吸酵素に及ぼす影響2016

    • Author(s)
      井上潤, 王孝ウェン, 牧野義雄, 大下誠一, 前島健作, 舟山幸子, 野口航
    • Organizer
      農業食料工学会関東支部第52回年次大会
    • Place of Presentation
      筑波大学(茨城県つくば市)
    • Year and Date
      2016-08-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] 貯蔵温度及びガス組成が収穫後ブロッコリーの呼吸酵素に及ぼす影響2016

    • Author(s)
      王孝ウェン, 牧野義雄, 大下誠一, 前島健作, 舟山(野口)幸子, 野口航
    • Organizer
      日本包装学会第25回年次大会研究発表会
    • Place of Presentation
      神戸大学(兵庫県神戸市)
    • Year and Date
      2016-07-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] USBカメラによる収穫後ブロッコリーの迅速外観品質評価2016

    • Author(s)
      大下誠一,牧野義雄,外13名
    • Organizer
      2016国際食品工業展アカデミックプラザ研究発表会
    • Place of Presentation
      東京ビッグサイト(東京都江東区)
    • Year and Date
      2016-06-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Respiratory enzyme activities and protein amounts of broccoli florets under modified atmospheres at different temperatures2016

    • Author(s)
      Wang H.W., Makino Y., Oshita S., Maejima K., Funayama S., Noguchi K.
    • Organizer
      第75回農業食料工学会年次大会
    • Place of Presentation
      京都大学(京都府京都市)
    • Year and Date
      2016-05-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] The effect of storage environment on the respiratory enzyme activity in broccoli2016

    • Author(s)
      Wang H.W., Makino Y., Oshita S., Maejima K., Funayama S.N., Noguchi K.
    • Organizer
      8th International Symposium on Machinery and Mechatronics for Agricultural and Biosystems Engineering
    • Place of Presentation
      朱鷺メッセ(新潟県新潟市)
    • Year and Date
      2016-05-24
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Nondestructive prediction of degreening rate of broccoli buds by photosensing2015

    • Author(s)
      Amino G., Makino Y., Oshita S.
    • Organizer
      2015 International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      ホノルル市(アメリカ合衆国)
    • Year and Date
      2015-12-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Nondestructive estimation of respiratory enzymatic activity associated with food loss during transportation and storage in brassicaceous vegetables2015

    • Author(s)
      Makino Y., Amino G., Nishimoto S., Oshita S.
    • Organizer
      Second International Conference on Global Food Security
    • Place of Presentation
      イサカ市(アメリカ合衆国)
    • Year and Date
      2015-10-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] USB カメラを利用した収穫後ブロッコリーの外観品質評価2015

    • Author(s)
      牧野義雄, 若月碧衣, 網野元貴, 大下誠一, 佐藤朱里, 塚田正人
    • Organizer
      農業環境工学関連5学会2015年合同大会
    • Place of Presentation
      岩手大学(岩手県、盛岡市)
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] USBカメラを利用した収穫後マンゴーの外観品質評価2015

    • Author(s)
      牧野義雄,後藤健次郎,大下誠一,佐藤朱里,塚田正人
    • Organizer
      日本生物環境工学会2015年宮崎大会
    • Place of Presentation
      シーガイアコンベンションセンター(宮崎県、宮崎市)
    • Year and Date
      2015-09-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] 天然色素濃度に基づくブロッコリー花蕾部外観品質の客観的評価2015

    • Author(s)
      牧野義雄,若月碧衣,網野元貴,大下誠一
    • Organizer
      日本食品工学会第16回年次大会
    • Place of Presentation
      広島市立大学(広島市)
    • Year and Date
      2015-08-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 光センシングによる農畜産物の非破壊品質評価2015

    • Author(s)
      牧野義雄
    • Organizer
      日本調理食品研究会年次大会(平成27年度)セミナー「調理食品分野における今最新の話題を探る!」
    • Place of Presentation
      大阪国際会議場(大阪市)
    • Year and Date
      2015-05-22
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Nondestructive prediction of cytochrome c oxidase activity in broccoli buds by spectroscopy2014

    • Author(s)
      Nishimoto S., Makino Y., Oshita S.
    • Organizer
      The International Conference on Plant Factory 2014
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2014-11-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] ブロッコリー花蕾部から抽出・精製したミトコンドリアの光吸収特性2014

    • Author(s)
      西本成潤,大下誠一,牧野義雄,有村慎一,高梨秀樹
    • Organizer
      農業機械学会関東支部第50回年次大会
    • Place of Presentation
      静岡県小笠山総合運動公園(静岡県、袋井市)
    • Year and Date
      2014-08-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] 青果物の鮮度低下における呼吸酵素の作用機作2014

    • Author(s)
      牧野義雄
    • Organizer
      日本食品保蔵科学会第63回大会(長野大会)シンポジウム「青果物の品質保持とその予測技術の最前線」
    • Place of Presentation
      JA長野県ビル(長野県、長野市)
    • Year and Date
      2014-06-29
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Effects of in-package atmosphere on temporal changes in the fractionation of oxygen isotopes during respiration in tomatoes2014

    • Author(s)
      Makino Y., Honda S., Oshita S., Noguchi K., Nakao S.
    • Organizer
      19th IAPRI World Conference on Packaging
    • Place of Presentation
      メルボルン(オーストラリア)
    • Year and Date
      2014-06-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Detection of cytochrome c oxidase in broccoli buds by spectroscopy and staining2014

    • Author(s)
      Nishimoto S., Makino Y., Oshita S.
    • Organizer
      7th International Symposium on Machinery and Mechatronics for Agricultural and Biosystems Engineering
    • Place of Presentation
      宜蘭(台湾)
    • Year and Date
      2014-05-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] ブロッコリー花蕾部におけるチトクロムcオキシダーゼ活性の分光分析による非破壊推定2014

    • Author(s)
      西本成潤,大下誠一,牧野義雄
    • Organizer
      第73回農業食料工学会年次大会
    • Place of Presentation
      琉球大学(沖縄県、中頭郡西原町)
    • Year and Date
      2014-05-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 青果物包装用高機能フィルムの特徴と使用効果について2014

    • Author(s)
      牧野義雄
    • Organizer
      新技術セミナー in 高松(高機能フィルムセミナー)
    • Place of Presentation
      サンポートホール高松、香川県高松市
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Oxygen consumption by horticultural products as a crucial factor in their deterioration during transportation and storage in the market2013

    • Author(s)
      Makino Y., Kosaka Y., Hosaka A., Nishimoto S., Oshita S.
    • Organizer
      First International Conference on Global Food Security
    • Place of Presentation
      ノールトウイーケルハウト、オランダ
    • Related Report
      2013 Annual Research Report
  • [Book] 機能性植物が秘めるビジネスチャンス~各社事例から学ぶ成分向上手法/事業活用例~2016

    • Author(s)
      牧野義雄(分担執筆)
    • Total Pages
      332
    • Publisher
      ㈱情報機構
    • Related Report
      2015 Annual Research Report
  • [Book] 農産食品プロセス工学2015

    • Author(s)
      牧野義雄(分担執筆)
    • Total Pages
      315
    • Publisher
      文永堂出版
    • Related Report
      2014 Annual Research Report

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Published: 2013-05-15   Modified: 2022-02-28  

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