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Regulation of genome reprogramming by the mobilization of maternal mRNA

Research Project

Project/Area Number 25252054
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Integrative animal science
Research InstitutionThe University of Tokyo

Principal Investigator

Aoki Fugaku  東京大学, 大学院新領域創成科学研究科, 教授 (20175160)

Project Period (FY) 2013-10-21 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥44,330,000 (Direct Cost: ¥34,100,000、Indirect Cost: ¥10,230,000)
Fiscal Year 2016: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2015: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2014: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
Fiscal Year 2013: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
Keywordsリプログラミング / 母性mRNA / 1細胞期胚 / 卵 / 初期胚 / 未受精卵 / 全能性 / ゲノムリプログラミング / 母性RNA / 受精卵 / 卵母細胞
Outline of Final Research Achievements

There are the maternal mRNA species which are not translated in immature oocytes but become being translated by elongation of their poly-A tails after the resumption of meiosis and fertilization. I hypothesized that the protein(s) regulating the reprogramming of gene expression is (are) translated from this type of maternal mRNAs. In this project, I planned to identify them and clarify their involvement in the reprogramming of gene expression.
First, we developed the system by which the maternal mRNAs elongating their poly-A tails were efficiently sorted. The selected mRNAs by using this system were subjected to RNA sequencing to determine the candidates for the factors involved in the reprogramming. The involvement of those candidates in the reprogramming has been examined by CRISPER/Cas9 system.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (25 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (6 results) Journal Article (10 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 10 results,  Open Access: 9 results,  Acknowledgement Compliant: 7 results) Presentation (9 results) (of which Int'l Joint Research: 1 results,  Invited: 5 results)

  • [Int'l Joint Research] Institute of Molecular Genetics(チェコ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Zagreb(Croatia)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Pennsylvania(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Pennsylvania(米国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Acad. of Sci. of the Czech Republic(チェコ)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Zagreb University(Croatia)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] A unique mechanism regulating gene expression in 1-cell embryos2017

    • Author(s)
      Yamamoto R, Aoki F
    • Journal Title

      Journal of Reproduction and Development

      Volume: 63 Issue: 1 Pages: 9-11

    • DOI

      10.1262/jrd.2016-133

    • NAID

      130005332263

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Regulation of zygotic gene activation by chromatin structure and epigenetic factors.2017

    • Author(s)
      Funaya S, Aoki F
    • Journal Title

      J Reprod Dev

      Volume: 印刷中

    • NAID

      130005982815

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Long non-coding RNA exchange during the oocyte-to-embryo transition in mice.2017

    • Author(s)
      Karlic R, Ganesh S, Franke V, Svobodova E, Urbanova J, Suzuki Y, Aoki F, Vlahovicek K, Svoboda P.
    • Journal Title

      DNA Res

      Volume: 24 Pages: 129-141

    • DOI

      10.1093/dnares/dsw058

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Analysis of chromatin structure in mouse preimplantation embryos by fluorescent recovery after photobleaching2016

    • Author(s)
      Ooga M, Fulka H, Hashimoto S, Suzuki MG, Aoki F
    • Journal Title

      Epigenetics

      Volume: 11 Issue: 1 Pages: 85-94

    • DOI

      10.1080/15592294.2015.1136774

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Nucleolus Precursor Bodies and Ribosome Biogenesis in Early Mammalian Embryos: Old Theories and New Discoveries2016

    • Author(s)
      Fulka H, Aoki F
    • Journal Title

      Biol Reprod

      Volume: 96 Issue: 6 Pages: 1-8

    • DOI

      10.1095/biolreprod.115.136093

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Characterization of gene expression in mouse embryos at the 1-cell stage2016

    • Author(s)
      Yamamoto R, Abe K, Suzuki Y, Suzuki MG, Aoki F.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 62 Issue: 1 Pages: 87-92

    • DOI

      10.1262/jrd.2015-131

    • NAID

      130005126315

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Analysis of chromatin structure in mouse preimplantation embryos by fluorescent recovery after photobleaching.2016

    • Author(s)
      Ooga, M., Fulka1, H., Hashimoto, S., Suzuki, M.G. and Aoki, F.
    • Journal Title

      Epigenetics

      Volume: 11 Pages: 85-94

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The first murine zygotic transcription is promiscuous and uncoupled from splicing and 3' processing.2015

    • Author(s)
      Abe, K., Yamamoto, R., Franke, V., Cao, M., Suzuki, Y., Suzuki, M.G., Vlahovicek, K., Svoboda, P., Schultz, R.M. and Aoki, F.
    • Journal Title

      EMBO J.

      Volume: 34 Issue: 11 Pages: 1523-1537

    • DOI

      10.15252/embj.201490648

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genome-wide analysis of the chromatin composition of histone H2A and H3 variants in mouse embryonic stem cells.2014

    • Author(s)
      Yukawa, M. et al.
    • Journal Title

      PLOS One

      Volume: 9 Issue: 3 Pages: e92689-e92689

    • DOI

      10.1371/journal.pone.0092689

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regulation of Transketolase Like 1 Gene Expression in the Murine One-Cell Stage Embryos.2014

    • Author(s)
      Hamamoto G, Suzuki T, Suzuki MG & Aoki F
    • Journal Title

      PLoS ONE

      Volume: 9 Issue: 1 Pages: e82087-e82087

    • DOI

      10.1371/journal.pone.0082087

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] マウス初期胚発生におけるminor ZGAの生物学的な役割2016

    • Author(s)
      阿部健一郎、鈴木穣、青木不学
    • Organizer
      第109回日本繁殖生物学会大会
    • Place of Presentation
      麻布大学(神奈川県相模原市)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] Involvement of histone variants replacement in the genome remodeling after fertilization.2016

    • Author(s)
      Aoki, F.
    • Organizer
      Joint Symposium on Integrated Biosciences between Zhejiang University and The University of Tokyo
    • Place of Presentation
      Hangzhou (China)
    • Year and Date
      2016-03-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 受精前後におけるゲノム再プログラム化の分子機構2016

    • Author(s)
      青木不学
    • Organizer
      第57回組織細胞化学会
    • Place of Presentation
      杏林大学(東京都三鷹市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 全能性とは?2015

    • Author(s)
      青木不学
    • Organizer
      第38回分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Characterization of gene expression in one-cell stage embryos2014

    • Author(s)
      Aoki F
    • Organizer
      World Congress of Reproductive Biology
    • Place of Presentation
      Edinburgh, England
    • Year and Date
      2014-09-02 – 2014-09-05
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] The role of histone H3 variants in the alteration of chromatin structure after fertilization.2014

    • Author(s)
      Kawamura M & Aoki F
    • Organizer
      World Congress of Reproductive Biology
    • Place of Presentation
      Edinburgh, England
    • Year and Date
      2014-09-02 – 2014-09-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] Roles of the untranslated regions in the translational regulation of cyclin A2 in murine oocytes and embryos.2014

    • Author(s)
      Mori M & Aoki F
    • Organizer
      World Congress of Reproductive Biology
    • Place of Presentation
      Edinburgh, England
    • Year and Date
      2014-09-02 – 2014-09-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] マウス初期胚発生においてCcnA2の非翻訳領域が翻訳量に及ぼす影響2014

    • Author(s)
      森優賀、青木不学
    • Organizer
      107回日本繁殖生物学会
    • Place of Presentation
      帯広畜産大学(帯広市)
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] 受精前後におけるエピジェネティック・リプログラミングについて2014

    • Author(s)
      青木不学
    • Organizer
      第20回ART FORUM
    • Place of Presentation
      ハイアットリージェンシー東京(東京都新宿区)
    • Year and Date
      2014-07-31
    • Related Report
      2014 Annual Research Report
    • Invited

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Published: 2013-10-24   Modified: 2022-02-21  

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