|Budget Amount *help
¥44,330,000 (Direct Cost: ¥34,100,000、Indirect Cost: ¥10,230,000)
Fiscal Year 2016: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2015: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2014: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
Fiscal Year 2013: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
|Outline of Final Research Achievements
There are the maternal mRNA species which are not translated in immature oocytes but become being translated by elongation of their poly-A tails after the resumption of meiosis and fertilization. I hypothesized that the protein(s) regulating the reprogramming of gene expression is (are) translated from this type of maternal mRNAs. In this project, I planned to identify them and clarify their involvement in the reprogramming of gene expression.
First, we developed the system by which the maternal mRNAs elongating their poly-A tails were efficiently sorted. The selected mRNAs by using this system were subjected to RNA sequencing to determine the candidates for the factors involved in the reprogramming. The involvement of those candidates in the reprogramming has been examined by CRISPER/Cas9 system.