|Budget Amount *help
¥46,280,000 (Direct Cost: ¥35,600,000、Indirect Cost: ¥10,680,000)
Fiscal Year 2016: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2015: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2014: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Fiscal Year 2013: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
|Outline of Final Research Achievements
The present study was aimed to promote the practical application of artificial nucleases, which has drawn attention as a new method of gene targeting organism production, and studied various technological developments and application to livestock eggs and fish eggs.
In this study, we showed that triple KO can be prodused at a time using CRISPR/Cas system, and that large-scale mutation can be introduced. As technology development, we constructed offset nicking system which is expected to suppress nonspecific effects and increased availability of original CRISPR/Cas system of other species with different PAM sequence. For the application, we showed the availability of this system to porcine eggs, Yamame and rainbow trout eggs.