| Budget Amount *help |
¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
Fiscal Year 2016: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2015: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2014: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2013: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
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| Outline of Final Research Achievements |
Our aim of this study is to reveal the gene network underlying the extreme desiccation tolerance in the desiccation tolerant cultured cells, Pv11, derived from the sleeping chironomid using the genome editing, such as CRISPR/Cas9.At first, we deciphered genome sequence of the sleeping chironomid to seek potential targets for CRISPR/Cas9, and consequently found about 2 million sites as the targets in the genome. Simultaneously, we developed a CRISPR/Cas9 applicable to Pv11 cells based on the originally isolated promoter from the cells. The optimized CRISPR/Cas9 allows us to conduct the genome-wide screening to identify the genes responsible to anhydrobiosis in Pv11 cells.
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