Functional roles of arginine methylation on vascular networks for nutritional supply
Project/Area Number |
25252062
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied molecular and cellular biology
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Research Institution | University of Tsukuba |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
ISHIDA Junji 筑波大学, 生命領域学際研究センター, 講師 (30323257)
KAKO Koichiro 筑波大学, 生命環境系, 講師 (60311594)
KIM Jun-Dal 筑波大学, 生命領域学際研究センター, 講師 (90570036)
SUGIYAMA Fumihiro 筑波大学, 医学医療系, 教授 (90226481)
KASUYA Yoshitoshi 千葉大学, 医学系研究科, 准教授 (70221877)
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥46,150,000 (Direct Cost: ¥35,500,000、Indirect Cost: ¥10,650,000)
Fiscal Year 2016: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2015: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2014: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2013: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
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Keywords | 栄養 / 血管 / PRMT1 / 内皮細胞特異的ノックアウトマウス / イメージング / 透明化 / 二光子顕微鏡 / 代謝 / 血管内皮細胞 / ノックアウトマウス / 栄養補給路 / アルギニンメチル化酵素 / 内皮細胞 |
Outline of Final Research Achievements |
Angiogenesis is important in embryonic development, and endothelial cells play a critical role in these processes. Protein arginine methyltransferase 1 (PRMT1) is involved in multiple cellular functions including proliferation and differentiation, and is expressed in vascular endothelial cells, which are responsible for angiogenesis during embryonic development. Since homozygous mutation of the Prmt1 gene results in early embryonic lethality in mice, little has been known about its physiological importance. In the present study, we found that the endothelial cell-specific PRMT1 deficient mice (PRMT1-ECKO) died within embryonic day 15. Macroscopic observation showed that the temporal arteries were poorly perfused with blood as compared with controls. Whole mount in vivo 3D imaging revealed the dilated and segmentalized luminal structures in PRMT1-ECKO fetuses. Our findings clearly provide evidence that PRMT1 is essential for normal vascular formation during embryogenesis.
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Report
(5 results)
Research Products
(31 results)
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[Journal Article] Angiodysplasia in embryo lacking protein arginine methyltransferase 1 (PRMT1) in vascular endothelial cells.2017
Author(s)
Ishimaru, T., Ishida, J., Kim, JD., Mizukami, H., Hara, K., Hashimoto, M., Yagami, KI., Sugiyama, F., and Fukamizu, A.
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Journal Title
Journal of Biochemistry
Volume: 161
Pages: 255-258
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Severe hypomyelination and developmental defects are caused in mice lacking protein arginine methyltransferase 1 (PRMT1) in the central nervous system.2016
Author(s)
Hashimoto, M., Murata, M., Ishida, J., Kanou, A., Kasuya, Y., and Fukamizu, A.
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Journal Title
J. Biol. Chem.
Volume: 291
Issue: 5
Pages: 2237-2245
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] PRMT8 as a phospholipase regulates Purkinje cell dendritic arborization and motor coordination.2015
Author(s)
Kim, J.D., Park, K.E., Ishida, J., Kako, K., Hamada, J., Kani, S., Takeuchi, M., Namiki, K., Fukui, H., Fukuhara, S., Hibi, M,. Kobayashi, M., Kanaho, Y., Kasuya, Y., Mochizuki, N., Fukamizu, A.
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Journal Title
Sci. Adv.
Volume: 1
Issue: 11
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] p38 mitogen-activated protein kinase accelerates emphysema in mouse model of chronic obstructive pulmonary disease.2014
Author(s)
Amano H, Murata K, Matsunaga H, Tanaka K, Yoshioka K, Kobayashi T, Ishida J, Fukamizu A, Sugiyama F, Sudo T, Kimura S, Tatsumi K, Kasuya Y.
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Journal Title
J Recept Signal Transduc
Volume: in press
Issue: 4
Pages: 299-306
DOI
Related Report
Peer Reviewed
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[Journal Article] Truncated Cables1 causes agenesis of the corpus callosum in mice2014
Author(s)
Mizuno S, Dinh T, Mizobuchi A, Iseki H, Mizuno-Iijima S, Kim J-D, Ishida J, Matsuda Y, Kunita S, Fukamizu A, Yagami K, Sugiyama F
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Journal Title
Laboratory Investigation
Volume: 94
Issue: 3
Pages: 321-330
DOI
Related Report
Peer Reviewed
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[Journal Article] Apelin is a positive regulator of ACE2 in failing hearts.2013
Author(s)
Sato T, Suzuki T, Watanabe H, K adowaki A, Fukamizu A, Liu PP, Kimura A, Ito H, Penninger JM, I mai Y, Kuba K.
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Journal Title
J Clin Invest
Volume: 123
Issue: 12
Pages: 5203-11
DOI
Related Report
Peer Reviewed
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[Presentation] エネルギー代謝と転写制御2016
Author(s)
深水 昭吉
Organizer
第11回 臨床ストレス応答学会大会
Place of Presentation
山口大学医学部霜仁会館(山口県 宇部市)
Year and Date
2016-11-11
Related Report
Invited
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[Presentation] 寿命を動かす代謝の仕組み2016
Author(s)
深水 昭吉
Organizer
第39回 日本基礎老化学会大会
Place of Presentation
伊勢原市民文化会館(神奈川県 伊勢原市)
Year and Date
2016-05-27
Related Report
Invited
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[Presentation] Dietary methionine restriction in axenic medium decreases both methionine and S-adenosyl-L-methionine levels2014
Author(s)
Yamauchi, R., Hirota, K., Xu, Z., Iizuka, K., Daitoku, H., Kako, K., Fukamizu, A.
Organizer
6th Asia-pacific C.elegans Meeting
Place of Presentation
奈良, 奈良県新公会堂
Year and Date
2014-07-15
Related Report
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[Presentation] Combinatorial treatment with antihypertensive drugs prevents HELLP syndrome exacerbated by endothelial dysfunction in pregnant hypertensive mice.2014
Author(s)
Junji Ishida, Tomohiro Ishimaru, Tanomu Matsukura, Shoko Nakamura, Haojun Xu, Altansarnai Baasanjav, Misuzu Hashimoto, Kazuya Murata, Katsumasa Nagano, Fumihiro Sugiyama, Ken-ichi Yagami, and Akiyoshi Fukamizu
Organizer
第18回 国際血管生物学会
Place of Presentation
京都府, みやこめっせ
Year and Date
2014-04-14
Related Report
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