Domain-to-domain coupling in voltage sensor domain proteins
| Project/Area Number |
25253016
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中川 敦史 大阪大学, たんぱく質研究所, 教授 (20188890)
藤原 祐一郎 大阪大学, 医学(系)研究科(研究院), 准教授 (20532980)
坂田 宗平 大阪大学, 医学(系)研究科(研究院), 招へい教員 (40528006)
筒井 秀和 大阪大学, 医学(系)研究科(研究院), 講師 (30392038)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥46,020,000 (Direct Cost: ¥35,400,000、Indirect Cost: ¥10,620,000)
Fiscal Year 2015: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2014: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2013: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
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| Keywords | イオンチャネル / 酵素 / 膜電位 / 電位センサー / イノシトールリン脂質 / ホスファターゼ / 不飽和脂肪酸 / 蛋白質モジュール / リン脂質 / 生体膜 |
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| Outline of Final Research Achievements |
Toward understanding general principle of domain-to-domain coupling in membrane proteins, we have exploited advantages of voltage-sensor domain proteins including voltage-gated proton channel (VSOP/Hv1) and voltage-sensing phosphatase. Approach of electrophysiology, voltage clamp fluorometery of cysteine-based method as well as fluorescent unnatural amino acid incorporation based on the genetic method enabled to reveal intermediate state of enzyme coupled to partially activated voltage sensor in VSP. X-ray crystal structure of voltage-gated proton channel was solved at 3.45 angstrom resolution as the zinc bound closed state, showing two S4 helices running straight next to each other within dimer in membrane which formed continuous helix down to the dimer cytoplasmic coiled coil at the C-terminus.
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Report
(4 results)
Research Products
(128 results)
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[Journal Article] Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging.2017
Author(s)
Inagaki S, Tsutsui H, Suzuki K, Agetsuma M, Arai Y, Jinno Y, Bai G, Daniels M, Okamura Y, Matsuda M, and Nagai T
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Journal Title
Scientific Reports
Volume: 7
Issue: 1
Pages: 42398-42398
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A Kir3.4 mutation causes Andersen–Tawil syndrome by an inhibitory effect on Kir2.1.2014
Author(s)
Kokunai Y, Nakata T, Furuta M, Sakata S, Kimura H, Aiba T, Yoshinaga M, Osaki Y, Nakamori M, Itoh H, Sato T, Kubota T, Kadota K, Shindo K, Mochizuki H, Shimizu W, Horie M, Okamura Y, Ohno K, Takahashi MP
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Journal Title
Neurology
Volume: 82
Issue: 12
Pages: 1058-64
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Structural studies of the novel protein family working with cell membrane potential signal2015
Author(s)
Atsushi Nakagawa, Hirotaka Narita, Yasushi Okamura, Akira Kawanabe, Yuichiro Fujiwara, Hidekazu Tsutsui, Hideki Kandori, Masayo Iwaki, Yu Takano, Hiroko Kondo, Yasushige Yonezawa, Kengo Kinoshita
Organizer
JST CREST-PREST joint international symposium Structural Biological Dynamics: From Molecules to Life with 60 trillion Cells
Place of Presentation
東京都文京区
Year and Date
2015-11-05
Related Report
Int'l Joint Research
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