| Budget Amount *help |
¥42,380,000 (Direct Cost: ¥32,600,000、Indirect Cost: ¥9,780,000)
Fiscal Year 2015: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2014: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2013: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
|
|---|
| Outline of Final Research Achievements |
We attempted to investigate the postsynaptic receptor dynamics during the formation of glycinergic inhibitory synapses by using electrophysiological and single molecular imaging approach. In spinal cord neurons cultured in the presence of strychinine, a glycine receptor antagonist, miniature inhibitory synaptic currents gradually increased in amplitude after wash-out strychinine. In addition, glycine tagged with Q dot moved much slower at the synaptic sites without strychinine compared with that with the receptor antagonist. In addition, lateral mobility of glycine receptor could be accelerated without glycine receptor. This suggests that glycine receptor at the inhibitory synapses could be limited in the mobility, resulting in the accumulation of glycine receptor at the glycinergic synapses. PKC activation upon the glycine receptor activation might regulate the receptor accumulation.
|
