| Budget Amount *help |
¥46,670,000 (Direct Cost: ¥35,900,000、Indirect Cost: ¥10,770,000)
Fiscal Year 2015: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2014: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2013: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
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| Outline of Final Research Achievements |
To dissect the molecular mechanism of Salmonella infection, we previously established the accurate prediction system for screening effectors on a genome-wide scale. Here, we characterized two candidates of novel effector, STM2614 and STM1239 and revealed their functions on Salmonella infection. STM2614 was injected into macrophage cytoplasm and activated caspase-8 which has two opposing functions namely as an initiator of an apoptosis and in a non-apoptotic role including induction of the pro-inflammatory response. As a molecule in macrophage targeted by STM2614, LSm8 was identified. STM1239 was injected into macrophage cells. As a molecule in macrophage targeted by STM1239, HO-1 (hem-oxygenase-1) was identified. STM1239 is capable of binding to the C-terminal transmembrane region of HO-1, suggesting the inhibition of its translocation into nucleus. It is suggested that STM1239 contributes the Salmonella infection by inhibiting the protective effect of HO-1 on its infection.
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