Studies on Salmonella infection and host response based on the interaction between effector and its host target
| Project/Area Number |
25253029
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Chiba University |
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Principal Investigator |
Yamamoto Tomoko 千葉大学, 真菌医学研究センター, 特任教授 (60110342)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAYA Akiko 千葉大学, 大学院薬学研究院, 准教授 (80334217)
佐藤 慶治 千葉大学, 薬学研究科(研究院), 助教 (00554586)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥46,670,000 (Direct Cost: ¥35,900,000、Indirect Cost: ¥10,770,000)
Fiscal Year 2015: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2014: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2013: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
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| Keywords | エフェクター / サルモネラ / 細菌病原性 / Caspase-8 / HO-1 / 宿主応答 / 感染免疫 / 感染生物学 / マクロファージ |
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| Outline of Final Research Achievements |
To dissect the molecular mechanism of Salmonella infection, we previously established the accurate prediction system for screening effectors on a genome-wide scale. Here, we characterized two candidates of novel effector, STM2614 and STM1239 and revealed their functions on Salmonella infection. STM2614 was injected into macrophage cytoplasm and activated caspase-8 which has two opposing functions namely as an initiator of an apoptosis and in a non-apoptotic role including induction of the pro-inflammatory response. As a molecule in macrophage targeted by STM2614, LSm8 was identified. STM1239 was injected into macrophage cells. As a molecule in macrophage targeted by STM1239, HO-1 (hem-oxygenase-1) was identified. STM1239 is capable of binding to the C-terminal transmembrane region of HO-1, suggesting the inhibition of its translocation into nucleus. It is suggested that STM1239 contributes the Salmonella infection by inhibiting the protective effect of HO-1 on its infection.
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] Putative classification of clades of enterohemorrhagic Escherichia coli O157 using an IS-printing system.2015
Author(s)
Hirai S, Yokoyama E, Etoh Y, Seto J, Ichihara S, Suzuki Y, Maeda E, Sera N,Horikawa K, Sato S, Yamamoto T.
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Journal Title
Lett Appl Microbiol.
Volume: 61
Issue: 3
Pages: 267-273
DOI
Related Report
Peer Reviewed / Open Access
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