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Basic construction for treatment effect prediction of molecular targeted drugs by quantitative estimation of HER family receptor based on highly sensitive nanoparticle imaging

Research Project

Project/Area Number 25253039
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionTohoku University

Principal Investigator

Ohuchi Noriaki  東北大学, 医学系研究科, 教授 (90203710)

Co-Investigator(Kenkyū-buntansha) 石田 孝宣  東北大学, 医学(系)研究科(研究院), 准教授 (00292318)
梅田 みか (渡辺みか / 梅田 みか(渡辺みか))  東北大学, 大学病院, 准教授 (20292344)
多田 寛  東北大学, 大学病院, 講師 (50436127)
宮下 穣  東北大学, 大学病院, 助教 (60710788)
権田 幸祐  東北大学, 医学(系)研究科(研究院), 教授 (80375435)
樋口 秀男  東京大学, 理学(系)研究科(研究院), 教授 (90165093)
甘利 正和  東北大学, 医学(系)研究科(研究院), 准教授 (50400312)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥46,150,000 (Direct Cost: ¥35,500,000、Indirect Cost: ¥10,650,000)
Fiscal Year 2016: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2015: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2014: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
Fiscal Year 2013: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Keywordsナノメディスン / 乳癌 / HERファミリー / 蛍光ナノ粒子 / FRET
Outline of Final Research Achievements

We developed new method for quantitative imaging of HER2/HER3 dimer by using fluorescent nanoparticle probes conjugated with antibodies. We performed detecting HER2/HER3 hetero-dimers by using the combination of antibody-based fluorescent probes which induce fluorescent resonance energy transfer (FRET). Because the efficiency of FRET is extremely sensitive to the distance between the pair of fluorophores, this method can be suitable for our purpose. We have applied this method for detection of HER2/HER3 hetero-dimers expressed on the membranes of human breast cancer cell lines. The amount of HER2/HER3 hetero-dimers and localization on cultured-cell lines have been evaluated with quantitative sensitivity. The imaging data showed HER2 and/or HER3-derived unique localization, and hot-spot on cancer cells.We also developed the evaluation method of the effect of antibody-drugs, especially anti-HER2 dimerization inhibitor, by FRET technique.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (11 results)

All 2017 2016 2015 2014

All Presentation (11 results) (of which Int'l Joint Research: 6 results)

  • [Presentation] maging of HER2/HER3 heterodimer on cancer cell membrane using FRET measurements2017

    • Author(s)
      Narufumi Kitamura, Kohsuke Gonda, Hiroshi Tada, Noriaki Ohuchi
    • Organizer
      A3 Conference on Nanoscale Imaging of Cancers
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2017-01-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Quantitative evaluation of HER2/HER3 dimerization and localization on cancer tissue sections2016

    • Author(s)
      Narufumi Kitamura, Kohsuke Gonda, Hiroshi Tada, Noriaki Ohuchi
    • Organizer
      A3 Symposium: Nanoscale Imaging and Tracing of Key Molecular Events in Cancer Biology Using Nanomaterials
    • Place of Presentation
      Beijing, China
    • Year and Date
      2016-07-31
    • Related Report
      2016 Annual Research Report
  • [Presentation] Quantitative imaging of HER2/HER3 heterodimers for pathological diagnosis of breast cancer2016

    • Author(s)
      北村成史、権田幸祐、多田寛、山本大輔、大内憲明
    • Organizer
      A3 Foresight 6th Meeting on Nanoscale Imaging of Cancers
    • Place of Presentation
      宮城、仙台
    • Year and Date
      2016-01-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] がん悪性度のマーカーであるHERファリミーダイマーの定量を指向した蛍光ナノ粒子プローブの開発2015

    • Author(s)
      北村成史、権田幸祐、多田寛、中野寧、大内憲明
    • Organizer
      第64回高分子討論会
    • Place of Presentation
      宮城、仙台
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] High accuracy imaging of cancer tissues with fluorescence nanoparticles and image-processing method2015

    • Author(s)
      権田幸祐、多田寛、北村成史、濱田庸、大内憲明
    • Organizer
      A3 Foresight 5th Meeting on Nanoscale Imaging of Cancers
    • Place of Presentation
      Jeju, Korea
    • Year and Date
      2015-08-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fluorescence nanoparticles for pathological diagnosis in breast cancer2015

    • Author(s)
      多田寛、権田幸祐、北村成史、宮下穣、石田孝宜、渡辺みか、大内憲明
    • Organizer
      A3 Foresight 4th Meeting on Nanoscale Imaging of Cancers
    • Place of Presentation
      Beijing, China
    • Year and Date
      2015-01-18
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of imaging of HER2/HER3 heterodimers with quantitative sensitivity for pathological diagnosis of breast cancer2014

    • Author(s)
      北村成史、権田幸祐、多田寛、大内憲明
    • Organizer
      8th International Symposium on Nanomedicine
    • Place of Presentation
      愛媛、松山
    • Year and Date
      2014-12-04
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fluorescence-based quantification of dimers of HER family for pathological diagnosis of breast cancer2014

    • Author(s)
      北村成史、権田幸祐、多田寛、渡辺みか、大内憲明
    • Organizer
      A3 Foresight 3rd Meeting on Nanoscale Imaging of Cancers
    • Place of Presentation
      宮城、仙台
    • Year and Date
      2014-09-02
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Potential clinical applications of fluorescence nanoparticles in breast cancer - imaging and molecular pathological diagnosis2014

    • Author(s)
      多田寛、権田幸祐、宮下穣、石田孝宣、渡辺みか、大内憲明
    • Organizer
      A3 Foresight 3rd Meeting on Nanoscale Imaging of Cancers
    • Place of Presentation
      宮城、仙台
    • Year and Date
      2014-09-02
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Nanotechnology based approaches in breast cancer-imaging and pathological diagnosis2014

    • Author(s)
      多田寛、権田幸祐、宮下穣、石田孝宣、渡辺みか、大内憲明
    • Organizer
      a3 Foresight Program Steering Meeting & A3 Conference on Nanoscale Imaging of Cancers
    • Place of Presentation
      Daejeon, Korea
    • Year and Date
      2014-02-20 – 2014-02-21
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of nano-size probes for quantification of biological event2014

    • Author(s)
      北村成史、岩田博夫、権田幸祐、多田寛、大内憲明
    • Organizer
      a3 Foresight Program Steering Meeting & A3 Conference on Nanoscale Imaging of Cancers
    • Place of Presentation
      Daejeon, Korea
    • Year and Date
      2014-02-20 – 2014-02-21
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-15   Modified: 2018-03-22  

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